FastQCFastQC Report
Sat 3 Sep 2022
EGAF00002490244

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002490244
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences490444
Sequences flagged as poor quality0
Sequence length35-151
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATGTAAATAATGCCTAGTTTTGAAGTAATGATTAACTGCATGTTCAGG30920.6304491440409099No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN24430.49812007079299575No Hit
TTATTAATAGATGTCAACAATATGTGGGCCCTCTTACAGTTAATGAAAAA23300.4750797236789521No Hit
CTCTTACAGTTAATGAAAAAAGGAGATTAAAATTAATTATGCCTGCTAGG18020.36742217256200504No Hit
TAACCTTACCAAATATTTACCCTTGGATAAAGGCATTAAACCTTATTATC17750.3619169568798884No Hit
CTTATATAAAATGCCAGCCTTCCACAGAGTATGTAAATAATGCCTAGTTT14850.3027868625164137No Hit
GTAATGTTAGGATAAAACCTAGCAGGCATAATTAATTTTAATCTCCTTTT14480.29524267806314275No Hit
CTATTAATAATGTCCTCATGTAAATGAATGTTAGGAAAGGAGGGAGTTTG14030.2860673185929484No Hit
ATCTCAATGTTAGTATCCCTTGGACTCATAAGGTGGGAAACTTTACTGGG12860.26221138397044313No Hit
ATATTTACCCTTGGATAAAGGCATTAAACCTTATTATCCTGAACATGCAG11480.23407361492851375No Hit
ATCTTAATGTCAGTATCCCTTGGACTCATAAGGTGGGAAACTTTACTGGG10460.21327613346273985No Hit
GTACAATATGTTCCTGTGGTAAAGTACCCCAACTTCCAATTACATATCCC9630.19635269266215916No Hit
GAGTATGTAAATAATGCCTAGTTTTGAAGTAATGATTAACTGCATGTTCA9110.18575005505215683No Hit
TATTAATAGATGTCAACAATATGTGGGCCCTCTTACAGTTAATGAAAAAA9070.1849344675436951No Hit
ACTCCGGATTAAAGATAGGTACAGTAGAAGAATAAAGCCCAGTAAAGTTT9030.18411888003523338No Hit
TCCACAGAGTATGTAAATAATGCCTAGTTTTGAAGTAATGATTAACTGCA8940.18228380814119452No Hit
ATATAAGACCTTGGGCAAGACCTGGTGGGCGTTCACGGTGGTCTCCATGC8480.17290455179388473No Hit
CCCTTAACTTCATGGGATATGTAATTGGAAGTTGGGGTACTTTACCACAG8420.17168117053119214No Hit
CTGTAAGAGGGCCCACATATTGTTGACATCTATTAATAATGTCCTCATGT8090.16495257358638296No Hit
GTGTAAAAGGGGCAGCAAAGCCCAAAAGACCCACAATTCTTTGACATACT7970.1625058110609978No Hit
CATTTGAACCCTAATAAAACCAAACGTTGGGGCTACTCCCTTAACTTCAT7880.1606707391669589No Hit
CTCTTATATAAAATGCCAGCCTTCCACAGAGTATGTAAATAATGCCTAGT7840.1598551516584972No Hit
ACAATGCAGGTCCCCTAGAAGAAGAACTCCCTCGCCTCGCAGACGAAGAT7700.15700059537888117No Hit
GTTTTGAAGTAATGATTAACTGCATGTTCAGGATAATAAGGTTTAATGCC7680.1565928016246503No Hit
GTGCTGGTGAACAGACCAATTTATGCCTACAGCCTCCTAGTACAAAGATC7640.1557772141161886No Hit
AGTCTAGACTCTGCGGTATTGTGAGGATTCTTGTCAACAAGAAAAACCCC7560.15414603909926516No Hit
TAGCCACACTGTGTAAAAGGGGCAGCAAAGCCCAAAAGACCCACAATTCT7360.15006810155695657No Hit
ACCAAATATTTACCCTTGGATAAAGGCATTAAACCTTATTATCCTGAACA7300.14884472029426396No Hit
ATATGTTCCTGTGGTAAAGTACCCCAACTTCCAATTACATATCCCATGAA7270.14823302966291768No Hit
ATGTCAGTATCCCTTGGACTCATAAGGTGGGAAACTTTACTGGGCTTTAT7230.14741744215445596No Hit
CTCCAAAAGACACCAAATACTCAAGAACAGTTTCTCTTCCAAAAGTAAGG7200.14680575152310968No Hit
ATTAATAGATGTCAACAATATGTGGGCCCTCTTACAGTTAATGAAAAAAG6980.14232002022657023No Hit
GGTCTATTTACAGGCAGTTTCCGAAAACATTGCTTGAGTTTCTGTACAAT6840.1394654639469542No Hit
CATTAAGATTCCCGAGATTGAGATCTTCTGCGACGCGGCGATTGAGATCT6770.13803818580714616No Hit
GGATAATAAGGTTTAATGCCTTTATCCAAGGGTAAATATTTGGTAATGTT6770.13803818580714616No Hit
GTCTTATATAAGAGGACTCTTGGACTCTCAGCAATGTCAACGACCGACCT6690.13640701079022272No Hit
AGTCTGTACAACATCTTGAGTCCCTTTTTACCTCTATTACCAATTTTCTT6570.13396024826483757No Hit
CTTACAGTTAATGAAAAAAGGAGATTAAAATTAATTATGCCTGCTAGGTT6560.13375635138772216No Hit
CATTATTAATAGATGTCAACAATATGTGGGCCCTCTTACAGTTAATGAAA6520.13294076387926043No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGGAGCACCCACG6460.13171738261656785No Hit
ATCCTAACATTACCAAATATTTACCCTTGGATAAAGGCATTAAACCTTAT6330.12906672321406726No Hit
CTATTACCAATTTTCTTTTGTCTTTGGGTATACATTTGAACCCTAATAAA6260.12763944507425926No Hit
CCTCCCGCTTACAGACCACCAAATGCCCCTATCTTATCAACACTTCCGGA6220.1268238575657975No Hit
AGTTAAGGGAGTAGCCCCAACGTTTGGTTTTATTAGGGTTCAAATGTATA6110.12458099191752778No Hit
ATCCTGAGTGGCAAACTCCCTCCTTTCCTAACATTCATTTACATGAGGAC6050.1233576106548352No Hit
GGTAAATATTTGGTAATGTTAGGATAAAACCTAGCAGGCATAATTAATTT5930.12091084812945005No Hit
GGATAAAGGCATTAAACCTTATTATCCTGAACATGCAGTTAATCATTACT5620.11459004493887173No Hit
ATATTGTACAGAAACTCAAGCAATGTTTTCGGAAACTGCCTGTAAATAGA5560.11336666367617913No Hit
AACCTTACCAAATATTTACCCTTGGATAAAGGCATTAAACCTTATTATCC5450.11112379802790939No Hit
TTATTATCCTGAACATGCAGTTAATCATTACTTCAAAACTAGGCATTATT5410.11030821051944768No Hit
ATTTACATACTCTGTGGAAGGCTGGCATTTTATATAAGAGAGAAACTACA5370.10949262301098597No Hit
ATGTAAATAATGCCTAGTTTTGAAGTAATGATTAACTGCATGTTCAGGAT5310.10826924174829337No Hit
CATGTATACAAGCTAAGCAGGCTTTCACTTTCTCGCCAACTTACAAGGCC5270.10745365423983166No Hit
ATTGACAACAGTGCCAGCAGCTCCTCCTCCTGCCTCCACCAATCGGCAGT5240.10684196360848537No Hit
GTCTATTTACAGGCAGTTTCCGAAAACATTGCTTGAGTTTCTGTACAATA5220.10643416985425451No Hit
TTGACATACTTTCCAATCAATAGGTCTATTTACAGGCAGTTTCCGAAAAC5210.10623027297713909No Hit
GGATAAAACCTAGCAGGCATAATTAATTTTAATCTCCTTTTTTCATTAAC5140.10480299483733106No Hit
GTTCAGGATAATAAGGTTTAATGCCTTTATCCAAGGGTAAATATTTGGTA5090.10378351045175391No Hit
CAGTTATATGGATGATGTGGTATTGGGGGCCAAGTCTGTACAACATCTTG5000.10194843855771504No Hit
GTGTAGTTTCTCTCTTATATAAAATGCCAGCCTTCCACAGAGTATGTAAA4970.10133674792636876No Hit
TCTGTGGAGTTACTCTCTTTTTTGCCTTCTGACTTCTTTCCTTCTATTCG4970.10133674792636876No Hit
CCCTTGGATAAAGGCATTAAACCTTATTATCCTGAACATGCAGTTAATCA4920.10031726354079161No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)