FastQCFastQC Report
Sat 3 Sep 2022
EGAF00002490245

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002490245
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences490444
Sequences flagged as poor quality0
Sequence length35-151
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATGTAAATAATGCCTAGTTTTGAAGTAATGATTAACTGCATGTTCAGG39670.8088589115169112No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN24320.495877205144726No Hit
TTATTAATAGATGTCAACAATATGTGGGCCCTCTTACAGTTAATGAAAAA22030.4491848202852925No Hit
CTCTTACAGTTAATGAAAAAAGGAGATTAAAATTAATTATGCCTGCTAGG16790.34234285667680714No Hit
GTAATGTTAGGATAAAACCTAGCAGGCATAATTAATTTTAATCTCCTTTT15030.3064570063044914No Hit
CTTATATAAAATGCCAGCCTTCCACAGAGTATGTAAATAATGCCTAGTTT14370.29299981241487305No Hit
TAACCTTACCAAATATTTACCCTTGGATAAAGGCATTAAACCTTATTATC14230.29014525613525705No Hit
CTATTAATAATGTCCTCATGTAAATGAATGTTAGGAAAGGAGGGAGTTTG13340.27199843407198376No Hit
ATCTCAATGTTAGTATCCCTTGGACTCATAAGGTGGGAAACTTTACTGGG13090.266901012144098No Hit
GAGTATGTAAATAATGCCTAGTTTTGAAGTAATGATTAACTGCATGTTCA10630.21674238037370222No Hit
GTGTAAAAGGGGCAGCAAAGCCCAAAAGACCCACAATTCTTTGACATACT10050.20491636150100725No Hit
ATATTTACCCTTGGATAAAGGCATTAAACCTTATTATCCTGAACATGCAG10040.20471246462389184No Hit
ATCTTAATGTCAGTATCCCTTGGACTCATAAGGTGGGAAACTTTACTGGG9670.1971682801706209No Hit
GTTTTGAAGTAATGATTAACTGCATGTTCAGGATAATAAGGTTTAATGCC9620.19614879578504374No Hit
CTCTTATATAAAATGCCAGCCTTCCACAGAGTATGTAAATAATGCCTAGT9480.19329423950542773No Hit
GTACAATATGTTCCTGTGGTAAAGTACCCCAACTTCCAATTACATATCCC9470.19309034262831232No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGGAGCACCCACG9300.18962409571735No Hit
CTGTAAGAGGGCCCACATATTGTTGACATCTATTAATAATGTCCTCATGT9290.18942019884023456No Hit
ACTCCGGATTAAAGATAGGTACAGTAGAAGAATAAAGCCCAGTAAAGTTT8600.1753513143192699No Hit
GTGCTGGTGAACAGACCAATTTATGCCTACAGCCTCCTAGTACAAAGATC8500.1733123455481156No Hit
TATTAATAGATGTCAACAATATGTGGGCCCTCTTACAGTTAATGAAAAAA8370.170661686145615No Hit
CCCTTAACTTCATGGGATATGTAATTGGAAGTTGGGGTACTTTACCACAG8360.17045778926849955No Hit
TCCACAGAGTATGTAAATAATGCCTAGTTTTGAAGTAATGATTAACTGCA8090.16495257358638296No Hit
CTCCAAAAGACACCAAATACTCAAGAACAGTTTCTCTTCCAAAAGTAAGG7870.16046684228984348No Hit
ATATGTTCCTGTGGTAAAGTACCCCAACTTCCAATTACATATCCCATGAA7410.1510875859425337No Hit
GGATAATAAGGTTTAATGCCTTTATCCAAGGGTAAATATTTGGTAATGTT7380.1504758953111874No Hit
AGTCTAGACTCTGCGGTATTGTGAGGATTCTTGTCAACAAGAAAAACCCC7060.14395119524349365No Hit
GTCTTATATAAGAGGACTCTTGGACTCTCAGCAATGTCAACGACCGACCT7020.14313560773503192No Hit
GGTCTATTTACAGGCAGTTTCCGAAAACATTGCTTGAGTTTCTGTACAAT6910.1408927420867622No Hit
CCTCCCGCTTACAGACCACCAAATGCCCCTATCTTATCAACACTTCCGGA6900.14068884520964678No Hit
TAGCCACACTGTGTAAAAGGGGCAGCAAAGCCCAAAAGACCCACAATTCT6840.1394654639469542No Hit
GTCTATTTACAGGCAGTTTCCGAAAACATTGCTTGAGTTTCTGTACAATA6800.13864987643849247No Hit
ATATAAGACCTTGGGCAAGACCTGGTGGGCGTTCACGGTGGTCTCCATGC6700.13661090766733816No Hit
CATTAAGATTCCCGAGATTGAGATCTTCTGCGACGCGGCGATTGAGATCT6620.13497973265041474No Hit
ATCCTGAGTGGCAAACTCCCTCCTTTCCTAACATTCATTTACATGAGGAC6570.13396024826483757No Hit
ATGTCAGTATCCCTTGGACTCATAAGGTGGGAAACTTTACTGGGCTTTAT6490.13232907324791413No Hit
CATTTGAACCCTAATAAAACCAAACGTTGGGGCTACTCCCTTAACTTCAT6370.12988231072252898No Hit
AGTCTGTACAACATCTTGAGTCCCTTTTTACCTCTATTACCAATTTTCTT6350.12947451696829812No Hit
ACAATGCAGGTCCCCTAGAAGAAGAACTCCCTCGCCTCGCAGACGAAGAT6340.1292706200911827No Hit
GTTCAGGATAATAAGGTTTAATGCCTTTATCCAAGGGTAAATATTTGGTA6230.12702775444291295No Hit
CTATTACCAATTTTCTTTTGTCTTTGGGTATACATTTGAACCCTAATAAA6170.12580437318022036No Hit
GGTAAATATTTGGTAATGTTAGGATAAAACCTAGCAGGCATAATTAATTT6160.12560047630310495No Hit
ATTAATAGATGTCAACAATATGTGGGCCCTCTTACAGTTAATGAAAAAAG6110.12458099191752778No Hit
CATTATTAATAGATGTCAACAATATGTGGGCCCTCTTACAGTTAATGAAA6020.12274592002348891No Hit
ATGTAAATAATGCCTAGTTTTGAAGTAATGATTAACTGCATGTTCAGGAT5990.12213422939214263No Hit
TTATTATCCTGAACATGCAGTTAATCATTACTTCAAAACTAGGCATTATT5950.12131864188368092No Hit
GTGTAGTTTCTCTCTTATATAAAATGCCAGCCTTCCACAGAGTATGTAAA5640.11499783869310257No Hit
ATCCTAACATTACCAAATATTTACCCTTGGATAAAGGCATTAAACCTTAT5580.11377445743041No Hit
AGTTAAGGGAGTAGCCCCAACGTTTGGTTTTATTAGGGTTCAAATGTATA5530.11275497304483284No Hit
ATGTGGTATTGGGGGCCAAGTCTGTACAACATCTTGAGTCCCTTTTTACC5480.1117354886592557No Hit
ATATTGTACAGAAACTCAAGCAATGTTTTCGGAAACTGCCTGTAAATAGA5410.11030821051944768No Hit
GGATAAAACCTAGCAGGCATAATTAATTTTAATCTCCTTTTTTCATTAAC5410.11030821051944768No Hit
CTTTTTACCTCTATTACCAATTTTCTTTTGTCTTTGGGTATACATTTGAA5290.10786144799406253No Hit
ATTTACATACTCTGTGGAAGGCTGGCATTTTATATAAGAGAGAAACTACA5210.10623027297713909No Hit
CTCTATTACCAATTTTCTTTTGTCTTTGGGTATACATTTGAACCCTAATA5140.10480299483733106No Hit
CTTACAGTTAATGAAAAAAGGAGATTAAAATTAATTATGCCTGCTAGGTT5110.10419130420598478No Hit
CAGTTATATGGATGATGTGGTATTGGGGGCCAAGTCTGTACAACATCTTG5080.10357961357463849No Hit
ACCAAATATTTACCCTTGGATAAAGGCATTAAACCTTATTATCCTGAACA5070.10337571669752307No Hit
CTCATAAGGTGGGAAACTTTACTGGGCTTTATTCTTCTACTGTACCTATC5060.10317181982040763No Hit
GATTAACTGCATGTTCAGGATAATAAGGTTTAATGCCTTTATCCAAGGGT4990.10174454168059963No Hit
GTACAACATCTTGAGTCCCTTTTTACCTCTATTACCAATTTTCTTTTGTC4980.10154064480348418No Hit
TTCTTCTACTGTACCTATCTTTAATCCGGAGTGGCAAACTCCCTCCTTTC4980.10154064480348418No Hit
GTGTGGCTATCCTGCCTTGATGCCTTTATATGCATGTATACAAGCTAAGC4980.10154064480348418No Hit
CTCCCATAGGAATCTTGCGAAAGCCCAGGATGATGGGATGGGAATACAAG4910.10011336666367618No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)