FastQCFastQC Report
Sun 4 Sep 2022
EGAF00002490247

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002490247
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences581090
Sequences flagged as poor quality0
Sequence length35-151
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGTAAAAGGGGCAGCAAAGCCCAAAAGACCCACAATTCTTTGACATACT22730.3911614379872309No Hit
GTATACATGCATATAAAGGCATTAAGGCAGGATAACCACATTGTGTAAAA19970.34366449259150905No Hit
GTATGTAAATAATGCCTAGTTTTGAAGTAATGATTAACTGCATGTTCAGG18950.3261112736409162No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN16360.28153986473695986No Hit
CCTCCCGCTTACAGACCACCAAATGCCCCTATCTTATCAACACTTCCGGA16260.2798189609182743No Hit
AGTCTGTACAACATCTTGAGTCCCTTTTTACCTCTATTACCAATTTTCTT15960.27465624946221756No Hit
CCCTTAATTTCATGGGATATGTAATTGGAAGCTGGGGTACTTTACCACAG15680.269837718769898No Hit
CTCTTACAGTTAATGAAAAAAGGAGATTAAAATTAATTATGCCTGCTAGG14990.2579634824209675No Hit
GCTTAGCTTGTATACATGCATATAAAGGCATTAAGGCAGGATAACCACAT14880.25607048822041334No Hit
ATCCTAACCTTACCAAATATTTGCCCTTGGATAAAGGCATCAAACCTTAT14700.2529728613467793No Hit
GTACAACATCTTGAGTCCCTTTTTACCTCTATTACCAATTTTCTTTTGTC14310.24626133645390558No Hit
TTATTAATAGATGTCAACAATATGTGGGCCCTCTTACAGTTAATGAAAAA14070.2421311672890602No Hit
CATTTGAACCCTAATAAAACCAAACGCTGGGGCTACTCCCTTAATTTCAT13870.23868935965168908No Hit
GTATTAAAACTCAAGCAATGTTTTCGGAAACTGCCTGTAAATAGGCCTAT13230.2276755752121014No Hit
GGTTAGGATAGAACCTAGCAGGCATAATTAATTTTAATCTCCTTTTTTCA12950.22285704451978178No Hit
ATGTGGTATTGGGGGCCAAGTCTGTACAACATCTTGAGTCCCTTTTTACC12340.21235953122579979No Hit
GTTTTAATACAATATGTTCCTGTGGTAAAGTACCCCAGCTTCCAATTACA12290.21149907931645703No Hit
ATATGTTCCTGTGGTAAAGTACCCCAGCTTCCAATTACATATCCCATGAA11930.2053038255691889No Hit
ATGTAATTGGAAGCTGGGGTACTTTACCACAGGAACATATTGTATTAAAA11710.20151783716808067No Hit
GGATAATAAGGTTTGATGCCTTTATCCAAGGGCAAATATTTGGTAAGGTT11530.19842021029444662No Hit
CTCCAAAAGACACCAAATACTCAAGAACAGTTTCTCTTCCAAAAGTAAGA10810.18602970279991052No Hit
CTTTTACACAATGTGGTTATCCTGCCTTAATGCCTTTATATGCATGTATA10790.1856855220361734No Hit
GGGTATGTAAATAATGCCTAGTTTTGAAGTAATGATTAACTGCATGTTCA10540.18138326248945946No Hit
CTATTAATAATGTCCTCCTGTAAATGAATGTTAGGAAAGGAGGGAGTTTG10430.17949026828890532No Hit
GTTATATGGATGATGTGGTATTGGGGGCCAAGTCTGTACAACATCTTGAG10310.17742518370648264No Hit
GGATAACCACATTGTGTAAAAGGGGCAGCAAAGCCCAAAAGACCCACAAT10230.1760484606515342No Hit
GGAACATATTGTATTAAAACTCAAGCAATGTTTTCGGAAACTGCCTGTAA10080.1734671049235058No Hit
CAATAGGCCTATTTACAGGCAGTTTCCGAAAACATTGCTTGAGTTTTAAT10050.17295083377790013No Hit
CAGTTATATGGATGATGTGGTATTGGGGGCCAAGTCTGTACAACATCTTG10040.1727787433960316No Hit
CTGTAAGAGGGCCCACATATTGTTGACATCTATTAATAATGTCCTCCTGT9820.16899275499492333No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGGAGCACCCACG9610.16537885697568364No Hit
TAACCACATTGTGTAAAAGGGGCAGCAAAGCCCAAAAGACCCACAATTCT9420.16210913972018104No Hit
CTTTTTACCTCTATTACCAATTTTCTTTTGTCTTTGGGCATACATTTGAA9380.1614207781927068No Hit
GTCTATAAGCGGGAGGAGTGCGAATCCACACTCCAAAAGACACCAAATAC9160.15763478979159856No Hit
AATTAAGGGAGTAGCCCCAGCGTTTGGTTTTATTAGGGTTCAAATGTATG8980.15453716291796452No Hit
CTTTAATCCTGAGTGGCAAACTCCCTCCTTTCCTAACATTCATTTACAGG8960.1541929821542274No Hit
GTATCGGGAGGCCTTAGAGTCTCCGGAACATTGTTCACCTCACCATACAG8900.15316043986301606No Hit
TTGACATACTTTCCAATCAATAGGCCTATTTACAGGCAGTTTCCGAAAAC8850.15229998795367328No Hit
AGTCTAGACTCTGTGGTATTGTGAGGATTCTTGTCAACAAGAAAAACCCC8670.14920236108003923No Hit
GTTCAAATGTATGCCCAAAGACAAAAGAAAATTGGTAATAGAGGTAAAAA8640.14868608993443355No Hit
CTACTGTACCTGTCTTTAATCCTGAGTGGCAAACTCCCTCCTTTCCTAAC8490.1461047342064052No Hit
GGATAGAACCTAGCAGGCATAATTAATTTTAATCTCCTTTTTTCATTAAC8390.14438383038771962No Hit
GTTCTATCCTAACCTTACCAAATATTTGCCCTTGGATAAAGGCATCAAAC8280.1424908361871655No Hit
GCCTTAGAGTCTCCGGAACATTGTTCACCTCACCATACAGCACTCAGGCA8100.13939320931353147No Hit
CTATTACCAATTTTCTTTTGTCTTTGGGCATACATTTGAACCCTAATAAA7990.13750021511297733No Hit
GTATTGGGGGCCAAGTCTGTACAACATCTTGAGTCCCTTTTTACCTCTAT7940.13663976320363455No Hit
TCCCATGGCTGCTCGGGTGTGCTGCCAACTGGATCCTGCGCGGGACGTCC7880.13560722091242322No Hit
ATTTACAGGCAGTTTCCGAAAACATTGCTTGAGTTTTAATACAATATGTT7800.13423049785747473No Hit
GCTTTGGGGCATGGACATTGACCCGTATAAAGAATTTGGAGCTTCTGTGG7790.13405840747560618No Hit
CAATCTAAGCAGGCTTTCACTTTCTCGCCAACTTACAAGGCCTTTCTCTG7700.13250959403878917No Hit
CATATAAAGGCATTAAGGCAGGATAACCACATTGTGTAAAAGGGGCAGCA7620.13113287098384072No Hit
TCTCATGTTCATGTCCTACTGTTCAAGCCTCCAAGCTGTGCCTTGGGTGG7470.12855151525581235No Hit
CTCTTTACGCGGTCTCCCCGTCTGTGCCTTCTCATCTGCCGGACCGTGTG7410.127518972964601No Hit
GTACAGGTGCATGTATTGTTGACAGAGAAAGGCCTTGTAAGTTGGCGAGA7320.12597015952778398No Hit
GCCCAAAAGACCCACAATTCTTTGACATACTTTCCAATCAATAGGCCTAT7310.12579806914591543No Hit
GGGTACAGGTGCATGTATTGTTGACAGAGAAAGGCCTTGTAAGTTGGCGA7270.1251097076184412No Hit
GCCATGGGAAGGAGGTGTATTTCCGAGAGAGGACAACAGAGTTGTCAGTC7230.12442134609096697No Hit
CTCCCATAGGAATCTTGCGAAAGCCCAGGATGATGGGATGGGAATACAAG7180.12356089418162419No Hit
ATACCACATCATCCATATAACTGAAAGCCAAACAGTGGGGGAAAGCCCTG7060.1214958095992015No Hit
CTTACAGACCACCAAATGCCCCTATCTTATCAACACTTCCGGAAACTACT7040.12115162883546439No Hit
GGCCTATTTACAGGCAGTTTCCGAAAACATTGCTTGAGTTTTAATACAAT6940.11943072501677882No Hit
GTGGTAAAGTACCCCAGCTTCCAATTACATATCCCATGAAATTAAGGGAG6920.1190865442530417No Hit
TTCTTCTACTGTACCTGTCTTTAATCCTGAGTGGCAAACTCCCTCCTTTC6870.11822609234369891No Hit
CTCTAAGGCCTCCCGATACAGAGCAGAGGCGGTGTCGAGGAGATCTCGAA6790.11684936928875046No Hit
GTTTACTAGTGCCATTTGTTCAGTGGTTCGCAGGGCTTTCCCCCACTGTT6730.1158168269975391No Hit
CAATTACATATCCCATGAAATTAAGGGAGTAGCCCCAGCGTTTGGTTTTA6720.11564473661567055No Hit
CTGTACAACATCTTGAGTCCCTTTTTACCTCTATTACCAATTTTCTTTTG6700.11530055585193344No Hit
GTTTTGAAGTAATGATTAACTGCATGTTCAGGATAATAAGGTTTGATGCC6680.11495637508819632No Hit
GTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATTGATGAGAGTCCAA6680.11495637508819632No Hit
CTCATAAGGTGGGAAACTTTACTGGGCTTTATTCTTCTACTGTACCTGTC6610.11375174241511643No Hit
CATGTATACAATCTAAGCAGGCTTTCACTTTCTCGCCAACTTACAAGGCC6580.11323547126951075No Hit
CTAGTAAACTGAGCCAGGAGAAACGGACTGAGGCCCACTCCCATAGGAAT6540.11254710974203652No Hit
TTCCCCCACTGTTTGGCTTTCAGTTATATGGATGATGTGGTATTGGGGGC6480.11151456745082516No Hit
ATTCTTTGACATACTTTCCAATCAATAGGCCTATTTACAGGCAGTTTCCG6460.11117038668708805No Hit
GTGCTGGTGAACAGACCAATTTATGCCTACAGCCTCCTAGTACAAAGACC6430.1106541155414824No Hit
CATCAATGTCAACGACCGACCTTGAGGCATACTTCAAAGACTGTTTGTTT6400.11013784439587672No Hit
ATACAATATGTTCCTGTGGTAAAGTACCCCAGCTTCCAATTACATATCCC6380.1097936636321396No Hit
GATTAACTGCATGTTCAGGATAATAAGGTTTGATGCCTTTATCCAAGGGC6360.10944948286840249No Hit
CTTTACCACAGGAACATATTGTATTAAAACTCAAGCAATGTTTTCGGAAA6330.10893321172279681No Hit
CTTTTATATAGAATGCCAGCCTTCCACAGGGTATGTAAATAATGCCTAGT6320.10876112134092825No Hit
GCATCAAACCTCGACAAGGCATGGGGACGAATCTTTCTGTTCCCAATCCT6280.10807275981345402No Hit
GTCTACGTCCCGTCGGCGCTGAATCCCGCGGACGACCCGTCTCGGGGCCG6270.10790066943158548No Hit
CCTCTATGTTTCCCTCTTGTTGCTGTACAAAACCTTCGGACGGAAACTGC6270.10790066943158548No Hit
GGCCTCTACCGTCCCCTTCTGCGTCTGCCGTTCCGGCCGACCACGGGGCG6100.10497513293982No Hit
CCCTTGGACTCATAAGGTGGGAAACTTTACTGGGCTTTATTCTTCTACTG6030.10377050026674009No Hit
GTTTGATGCTGTAGCTCTTGTTCCCAAGAATATGGTGACCCACAAAATGA5930.1020495964480545No Hit
GTGGTATTGGGGGCCAAGTCTGTACAACATCTTGAGTCCCTTTTTACCTC5920.10187750606618597No Hit
GTTCAGGATAATAAGGTTTGATGCCTTTATCCAAGGGCAAATATTTGGTA5910.1017054156843174No Hit
TCCTTTGTCTACGTCCCGTCGGCGCTGAATCCCGCGGACGACCCGTCTCG5850.10067287339310606No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)