FastQCFastQC Report
Sat 3 Sep 2022
EGAF00002490248

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002490248
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences343199
Sequences flagged as poor quality0
Sequence length35-151
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN14080.41025760564570407No Hit
GTATGTAAATAATGCCTAGTTTTGAAGTAATGATTAACTGCATGTTCAGG14050.40938347722458396No Hit
ATATAAGACCTTGGGCAAGACCTGGTGGGCGTTCACGGTGGTCTCCATGC11870.34586347862318945No Hit
ATCCTAACCTTACCAAATATTTGCCCTTGGATAAAGGCATTAAACCCTAT11060.32226201125294657No Hit
CCTCCCGGTTATAGACCACCAAATGCCCCTATCTTATCAACACTTCCGGA9450.2753504526528341No Hit
GTCTTATATAAGAGGACTCTTGGACTCTCAGCAATGTCAACGACCGACCT9250.2695229298453667No Hit
GGCATATAAAGGCATTAAGGCAGGATAGCCACATTGTGTAAAAGGGGCAG8870.25845063651117867No Hit
GTGCTGGTGAACAGACCAATTTATGCCTACAGCCTCCTAGTACAAAGATC8750.2549541228266982No Hit
CTTATATAGAATACCAGCCTTCCACAGAGTATGTAAATAATGCCTAGTTT8720.2540799944055781No Hit
CTCCAAAAGACACCAAATACTCAAGAACAGTTTCTCTTCCAAAAGTAAGA8200.23892843510616288No Hit
CTCTTACAGTTAATGAAAAAAGGAGATTAAAATTAATTATGCCTGCTAGG8040.23426641686018898No Hit
GGTTAGGATAGAACCTAGCAGGCATAATTAATTTTAATCTCCTTTTTTCA7450.2170752245781602No Hit
TTATTAATAGATGTCAACAATATGTGGGCCCTCTTACAGTTAATGAAAAA7030.20483742668247867No Hit
GTGTAAAAGGGGCAGCAAAGCCCAAAAGACCCACAATTCTTTGACACACT6900.20104953685762486No Hit
ATTAACAACAGTGCCAGCAGCACCTCCTCCTGCCTCCACCAATCGGCAGT6620.1928910049271705No Hit
CCAAAAGACACCAAATACTCAAGAACAGTTTCTCTTCCAAAAGTAAGACA6470.18852036282157No Hit
CTATTAATAATGTCCTCCTGTAAATGAATGTTAGGAAAGGAGGGAGTTTG6440.1876462344004499No Hit
CTCTTATATAGAATACCAGCCTTCCACAGAGTATGTAAATAATGCCTAGT6410.18677210597932978No Hit
AGTCTGTACAACATCTTGAGTCCCTTTTTACCTCTATTACCAATTTTCTT6330.1844410968563428No Hit
GTTGTTAATATGCCCTGAGCCTGAGGGCTCCACCCCAAAAGACCGCCGTG6320.18414972071596944No Hit
GGGTAAAGGTGCAGATATTGTTTACACAGAAAGGCCTTATAAGTTGGCGA6100.17773944562775534No Hit
GTATACAGGCATATAAAGGCATTAAGGCAGGATAGCCACATTGTGTAAAA6040.1759911887855151No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGGAGCACCCAAG5950.17336880352215478No Hit
GGATAATAGGGTTTAATGCCTTTATCCAAGGGCAAATATTTGGTAAGGTT5950.17336880352215478No Hit
CTTTCCAATCAATAGGTCTATTTACAGGCAGTTTTCGAAAACATTGCTTG5950.17336880352215478No Hit
GTTCTATCCTAACCTTACCAAATATTTGCCCTTGGATAAAGGCATTAAAC5780.1684154091358075No Hit
TTATATGCCTGTATACAATCAAAACAGGCTTTCACTTTCTCGCCAACTTA5690.16579302387244718No Hit
CCCTTAACTTCATGGGATATGTAATTGGAAGTTGGGGTACTTTACCACAA5650.1646275193109537No Hit
ATGTTAATATGGGTCTAAAAATCAGACAACTATTGTGGTTTCACATTTCC5600.16317063860908687No Hit
GTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATTGCTGAGAGTCCAA5600.16317063860908687No Hit
GTCTATTTACAGGCAGTTTTCGAAAACATTGCTTGAGTTTTTGTACAATA5580.16258788632834012No Hit
AGTCTAGACTCTGTGGTATTGTGAGGATTCTTGTCAACAAGAAAAACCCC5580.16258788632834012No Hit
CTTATAAGGCCTTTCTGTGTAAACAATATCTGCACCTTTACCCCGTTGCC5150.15005871229228523No Hit
GTATAAAGAATTTGGAGCTTCTGTGGAATTACTCTCATTTTTGCCTTCTG5050.14714495088855153No Hit
CCTGTATACAATCAAAACAGGCTTTCACTTTCTCGCCAACTTATAAGGCC5050.14714495088855153No Hit
CTGTAAGAGGGCCCACATATTGTTGACATCTATTAATAATGTCCTCCTGT4990.14539669404631134No Hit
GTACAACATCTTGAGTCCCTTTTTACCTCTATTACCAATTTTCTTTTGTC4970.14481394176556459No Hit
TCCACAGAGTATGTAAATAATGCCTAGTTTTGAAGTAATGATTAACTGCA4760.13869504281772382No Hit
TCCCATGGCTGCTCGGGTGTGCTGCCAACTGGATCCTGCGCGGGACGTCC4730.13782091439660374No Hit
CTTTTACACAATGTGGCTATCCTGCCTTAATGCCTTTATATGCCTGTATA4710.137238162115857No Hit
GTTAATATGGGTCTAAAAATCAGACAACTATTGTGGTTTCACATTTCCTG4700.13694678597548363No Hit
GTTTTATTAGGGTTCAAATGTATACCCAAAGACAAAAGAAAATTGGTAAT4630.13490715299287No Hit
CCTTTACCCCGTTGCCAGGCAACGGGCCGGTCTCTGCCAAGTGTTTGCTG4630.13490715299287No Hit
ATATGTTCTTGTGGTAAAGTACCCCAACTTCCAATTACATATCCCATGAA4580.13345027229100317No Hit
GCCATGGGAAGGAGGTGTATTTCCGAGAAAGGACAACAGAGTTGTCAGTC4560.13286752001025642No Hit
GTATCGGGAGGCCTTAGAGTCTCCGGAACATTGTTCACCTCACCATACAG4480.13053651088726947No Hit
CTTTAATCCTGAGTGGCAAACTCCCTCCTTTCCTAACATTCATTTACAGG4440.12937100632577603No Hit
CATTTGAACCCTAATAAAACCAAACGATGGGGTTACTCCCTTAACTTCAT4410.12849687790465592No Hit
CTCTTATATAAGACCTTGGGCAAGACCTGGTGGGCGTTCACGGTGGTCTC4380.12762274948353577No Hit
GTCTATAACCGGGAGGAGTGCGAATCCACACTCCAAAAGACACCAAATAC4350.12674862106241566No Hit
CTCAAGAACAGTTTCTCTTCCAAAAGTAAGACAGGAAATGTGAAACCACA4320.12587449264129558No Hit
CAGTTATATGGATGATGTGGTATTGGGGGCCAAGTCTGTACAACATCTTG4270.12441761193942873No Hit
ATATAAGAGAGAAACTACACGCAGCGCCTCATTTTGGGGGTCACCATATT4270.12441761193942873No Hit
GGATAGAACCTAGCAGGCATAATTAATTTTAATCTCCTTTTTTCATTAAC4250.12383485965868199No Hit
GGGTTACTCCCTTAACTTCATGGGATATGTAATTGGAAGTTGGGGTACTT4220.12296073123756188No Hit
CTCCCATAGGAATCTTGCGAAAGCCCAGGATGATGGGATGGGAATACAAG4220.12296073123756188No Hit
ACATTGACCCGTATAAAGAATTTGGAGCTTCTGTGGAATTACTCTCATTT4170.12150385053569504No Hit
CAATAGGTCTATTTACAGGCAGTTTTCGAAAACATTGCTTGAGTTTTTGT4150.1209210982549483No Hit
CTTATATAAGAGGACTCTTGGACTCTCAGCAATGTCAACGACCGACCTTG4080.1188814652723347No Hit
GTTTTGAAGTAATGATTAACTGCATGTTCAGGATAATAGGGTTTAATGCC4060.11829871299158796No Hit
ATGTGGTATTGGGGGCCAAGTCTGTACAACATCTTGAGTCCCTTTTTACC4040.11771596071084123No Hit
GGTCTATTTACAGGCAGTTTTCGAAAACATTGCTTGAGTTTTTGTACAAT4040.11771596071084123No Hit
TCCCGATACAGAGCAGAGGCGGTGTCGAGGAGATCTCGAATAGAAGGAAA4010.11684183228972111No Hit
GTCCGGCAGATGAGAAGGCACAGACGGGGAGACCGCGTAAAGAGAGGTGC3890.11334531860524069No Hit
GCCTTAGAGTCTCCGGAACATTGTTCACCTCACCATACAGCACTCAGGCA3880.11305394246486733No Hit
CTTTTTACCTCTATTACCAATTTTCTTTTGTCTTTGGGTATACATTTGAA3880.11305394246486733No Hit
CCTCTATGTTTCCCTCTTGTTGCTGTACAAAACCTTCGGACGGAAACTGC3870.11276256632449395No Hit
CTATATAAGAGAGAAACTACACGCAGCGCCTCATTTTGGGGGTCACCATA3850.11217981404374722No Hit
CTCATAAGGTGGGAAACTTTACTGGGCTTTATTCTTCTACCGTACCTATC3810.11101430948225373No Hit
GTTATATGGATGATGTGGTATTGGGGGCCAAGTCTGTACAACATCTTGAG3800.11072293334188035No Hit
CTATTGTGGTTTCACATTTCCTGTCTTACTTTTGGAAGAGAAACTGTTCT3790.11043155720150699No Hit
CTCTAAGGCCTCCCGATACAGAGCAGAGGCGGTGTCGAGGAGATCTCGAA3790.11043155720150699No Hit
GTTCAAATGTATACCCAAAGACAAAAGAAAATTGGTAATAGAGGTAAAAA3760.10955742878038688No Hit
CTCTTTACGCGGTCTCCCCGTCTGTGCCTTCTCATCTGCCGGACCGTGTG3750.10926605264001352No Hit
GTTATAGACCACCAAATGCCCCTATCTTATCAACACTTCCGGAAACTACT3740.10897467649964014No Hit
GCACGGGACCATGCAAGACCTGCACGAATCCTGCTCAAAACACCTCTATG3740.10897467649964014No Hit
GTCCCGTGCTGGTAGTTGATGTTCCTGGAAGTAGAGGACAAACGGGCAAC3740.10897467649964014No Hit
GTATACATTTGAACCCTAATAAAACCAAACGATGGGGTTACTCCCTTAAC3730.10868330035926678No Hit
GAGTATGTAAATAATGCCTAGTTTTGAAGTAATGATTAACTGCATGTTCA3720.1083919242188934No Hit
CTTTTGGAGTGTGGATTCGCACTCCTCCCGGTTATAGACCACCAAATGCC3700.10780917193814667No Hit
GGATTAAAGATAGGTACGGTAGAAGAATAAAGCCCAGTAAAGTTTCCCAC3660.10664366737665319No Hit
GCACTCAGGCAAGCTATTCTGTGTTGGGGTGAGTTGATGAATCTGGCCAC3660.10664366737665319No Hit
GTCCAAGAGTCCTCTTATATAAGACCTTGGGCAAGACCTGGTGGGCGTTC3640.10606091509590646No Hit
GTTCAGGATAATAGGGTTTAATGCCTTTATCCAAGGGCAAATATTTGGTA3630.1057695389555331No Hit
CTATTATCCTGAACATGCAGTTAATCATTACTTCAAAACTAGGCATTATT3620.10547816281515972No Hit
ATGTAATTGGAAGTTGGGGTACTTTACCACAAGAACATATTGTACAAAAA3600.10489541053441298No Hit
ATTTATGCCTACAGCCTCCTAGTACAAAGATCATTAACCTAATCTCCTCC3570.10402128211329288No Hit
GTACAATATGTTCTTGTGGTAAAGTACCCCAACTTCCAATTACATATCCC3560.1037299059729195No Hit
ATATAAGAGGACTCTTGGACTCTCAGCAATGTCAACGACCGACCTTGAGG3520.10256440141142602No Hit
ATTTACAGGCAGTTTTCGAAAACATTGCTTGAGTTTTTGTACAATATGTT3510.10227302527105264No Hit
TGTTAATATGGGTCTAAAAATCAGACAACTATTGTGGTTTCACATTTCCT3470.10110752070955917No Hit
GCTATGTCAATGTTAATATGGGTCTAAAAATCAGACAACTATTGTGGTTT3440.10023339228843907No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)