FastQCFastQC Report
Sat 3 Sep 2022
EGAF00002490251

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002490251
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences366633
Sequences flagged as poor quality0
Sequence length35-151
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATGTAAATAATGCCTAGTTTTGAAGTAATGATTAACTGCATGGTCAGG15700.4282211366680031No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN13780.3758526919289862No Hit
ATCCTAACCTTACCAAATATTTGCCCTTGGATAAAGGTATTAAACCTTAT12830.34994122187582677No Hit
CTCTTACAGTTAATGAAAAAAGGAGATTAAAACTAATTATGCCTGCTAGG11520.31421066843410167No Hit
CCTATATAGAATGCCAGCCTTCCACAGAGTATGTAAATAATGCCTAGTTT11220.30602809894363026No Hit
GTGTAAAAGGGGCAGCAAAGCCCAAAAGACCCACAATTCTTTGACATACT9230.25175038799017No Hit
AGTCTAGACTCTGTGGTATTGTGAGGATTCTTGTCAACAAGAAAAACCCC8790.23974928607081195No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGGAGCACCCACG8530.23265772584573674No Hit
GGATAATAAGGTTTAATACCTTTATCCAAGGGCAAATATTTGGTAAGGTT8390.2288391934168501No Hit
CCCTTAACTTCATGGGATATGTAATTGGAAGTTGGGGAACTTTACCACAG7880.2149288252830487No Hit
TTATTATCCTGACCATGCAGTTAATCATTACTTCAAAACTAGGCATTATT7290.19883643861845496No Hit
GTTCTATCCTAACCTTACCAAATATTTGCCCTTGGATAAAGGTATTAAAC7230.1971999247203607No Hit
CTCCTATATAGAATGCCAGCCTTCCACAGAGTATGTAAATAATGCCTAGT7170.19556341082226641No Hit
GTATACATGCATATAAAGGCATCAAGGCAGGATAGCCACATTGTGTAAAA6990.19065386912798357No Hit
ATATGTTCCTGTGGTAAAGTTCCCCAACTTCCAATTACATATCCCATGAA6840.18656258438274786No Hit
GGTTAGGATAGAACCTAGCAGGCATAATTAGTTTTAATCTCCTTTTTTCA6370.17374322551434268No Hit
CTATATAGGAGAGAAACTACACGTAGCGCCTCATTTTGTGGGTCACCATA6250.17047019771815414No Hit
AGTCTGTACAACATCTTGAGTCCCTTTTTACCTCTATTACCAATTTTCTT6080.16583340834022034No Hit
ATTGACAACAGTGCCAGCAGCACCTCCTCCTGCCTCCACCAATCGGCAGT5580.152195792522768No Hit
TCTGTGGAGTTACTCTCTTTTTTGCCTTCTGACTTCTTTCCTTCTATTCG5560.1516502878900699No Hit
GGGTAAAGGTGCAGATATTGTTTACACAGAAAGGCCTTGTAAGTTGGCGA5490.14974102167562658No Hit
CCCCAAAAGATACCAAATACTCAAGAACAGTTTCTCTTCCAAAAGTAAGA5470.1491955170429285No Hit
ATGTGGTATTGGGGGCCAAGTCTGTACAACATCTTGAGTCCCTTTTTACC5440.14837726009388133No Hit
CATTTGAACCCTAATAAAACCAAACGTTGGGGCTACTCCCTTAACTTCAT5340.14564973693039088No Hit
CTTTTACACAATGTGGCTATCCTGCCTTGATGCCTTTATATGCATGTATA5140.14019469060340994No Hit
ATATGTGGGTCCTCTTACAGTTAATGAAAAAAGGAGATTAAAACTAATTA5120.13964918597071185No Hit
GAGTATGTAAATAATGCCTAGTTTTGAAGTAATGATTAACTGCATGGTCA5100.13910368133801376No Hit
GGAACATATTGTACAAAAACTCAAGCAATGTTTTCGAAAACTGCCTGTAA5080.13855817670531567No Hit
CTTCTGGACTACCAAGGTATGTTGCCCGTTTGTCCTCTACTTCCAGGAAC4960.1352851489091271No Hit
CAATATGTGGGTCCTCTTACAGTTAATGAAAAAAGGAGATTAAAACTAAT4860.13255762574563665No Hit
GTTGTCAATATGCCCTGAGCCTGAGGGCTCCACCCCAAAAGACCGCCGTG4850.1322848734292876No Hit
CATGTATACAAGCTAAGCAGGCTTTCACTTTCTCGCCAACTTACAAGGCC4840.13201212111293856No Hit
GTACAACATCTTGAGTCCCTTTTTACCTCTATTACCAATTTTCTTTTGTC4740.12928459794944808No Hit
GTCTATTTACAGGCAGTTTTCGAAAACATTGCTTGAGTTTTTGTACAATA4740.12928459794944808No Hit
GTGTAGTTTCTCTCCTATATAGAATGCCAGCCTTCCACAGAGTATGTAAA4660.12710257941865571No Hit
GTCCACAAGAACCAACAAGAAGATGAGGCATAGCAGCAGGATGAAGAGGA4540.12382955162246714No Hit
TTATTAATAGATGTCAACAATATGTGGGTCCTCTTACAGTTAATGAAAAA4530.12355679930611811No Hit
GTATTAAACCTTATTATCCTGACCATGCAGTTAATCATTACTTCAAAACT4480.12219303772437286No Hit
GTTTAATACCTTTATCCAAGGGCAAATATTTGGTAAGGTTAGGATAGAAC4460.12164753309167478No Hit
GTATCGGGAGGCCTTAGAGTCTCCGGAACATTGTTCACCTCACCATACAG4440.12110202845897669No Hit
ATTTACATACTCTGTGGAAGGCTGGCATTCTATATAGGAGAGAAACTACA4420.1205565238262786No Hit
CAGTTATATGGATGATGTGGTATTGGGGGCCAAGTCTGTACAACATCTTG4390.11973826687723145No Hit
GTGCTGGTGAACAGACCAATTTATGCCTACAGCCTCCTAGTACAAAGATC4290.117010743713741No Hit
CTCCCATAGGAATCTTGCGAAAGCCCAGGATGATGGGATGGGAATACAAG4180.11401046823390149No Hit
GCCTTAGAGTCTCCGGAACATTGTTCACCTCACCATACAGCACTCAGGCA4170.11373771591755243No Hit
TTGACATACTTTCCAATCAATAGGTCTATTTACAGGCAGTTTTCGAAAAC4160.11346496360120338No Hit
GCTTTGGGGCATGGACATTGACCCGTATAAAGAATTTGGAGCTTCTGTGG4120.1123739543358072No Hit
GTACAATATGTTCCTGTGGTAAAGTTCCCCAACTTCCAATTACATATCCC4120.1123739543358072No Hit
TCCACAGAGTATGTAAATAATGCCTAGTTTTGAAGTAATGATTAACTGCA4060.11073744043771291No Hit
ATATAGGAGAGAAACTACACGTAGCGCCTCATTTTGTGGGTCACCATATT4030.10991918348866579No Hit
ATACCACATCATCCATATAACTGAAAGCCAAACAGTGGGGGAAAGCCCTA3940.10746441264152436No Hit
CATATAAAGGCATCAAGGCAGGATAGCCACATTGTGTAAAAGGGGCAGCA3930.1071916603251753No Hit
AGTTAAGGGAGTAGCCCCAACGTTTGGTTTTATTAGGGTTCAAATGTATA3910.10664615569247722No Hit
CCTTTATCCAAGGGCAAATATTTGGTAAGGTTAGGATAGAACCTAGCAGG3880.10582789874343007No Hit
GGATATTATTAATAGATGTCAACAATATGTGGGTCCTCTTACAGTTAATG3870.10555514642708103No Hit
CCTTTACCCCGTTGCCCGGCAACGGTCCGGTCTCTGCCAAGTGTTTGCTG3770.10282762326359056No Hit
GGTCTATTTACAGGCAGTTTTCGAAAACATTGCTTGAGTTTTTGTACAAT3710.10119110936549627No Hit
CAATAGGTCTATTTACAGGCAGTTTTCGAAAACATTGCTTGAGTTTTTGT3690.1006456047327982No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)