FastQCFastQC Report
Sat 3 Sep 2022
EGAF00002490252

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002490252
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences516989
Sequences flagged as poor quality0
Sequence length35-151
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATGTAAATAATGCCTAGTTTTGAAGTAATGATTAACTGCATTTTCAGG31280.6050418867712853No Hit
ATCCTAACCTTACCAAATATTTGCCCTTGGATAAAGGCATTAAACCGTAT30200.5841516937497704No Hit
CTTATATAGAATGCCAGCCTTCCACAGAGTATGTAAATAATGCCTAGTTT24140.46693449957349187No Hit
CTCTTACAGTTAATGAAAAAAGGAGATTAAAATTAATTATGCCTGCTAGG19820.38337372748743204No Hit
GTGTAAAGGGGGCAGCAAAGCCCAAAAGACCCACAATTCTTTGACATACT13960.27002508757439714No Hit
GTTCTATCCTAACCTTACCAAATATTTGCCCTTGGATAAAGGCATTAAAC13340.25803256935834223No Hit
CTCTTATATAGAATGCCAGCCTTCCACAGAGTATGTAAATAATGCCTAGT12870.24894146683972002No Hit
CCCTTAACTTCATGGGATATGTAATTGGAAGTTGGGGTACTTTACCGCAA12820.2479743282739091No Hit
GTGCTGGTGAACAGACCAATTTATGCCTACAGCCTCCTAGTACAAAGATC12130.234627816065719No Hit
ATCTTAATGTTAGTATCCCTTGGACTCATAAGGTGGGAAACTTTACTGGG11480.2220550147101776No Hit
AGTCTGTACAACATCTTGAGTCCCTTTTTACCTCTATTACCAATTTTCTT11350.21954045443906928No Hit
ATCTAAATGTTAGTATCCCTTGGACTCATAAGGTGGGAAACTTTACTGGG11300.21857331587325843No Hit
GGTTAGGATAGAACCTAGCAGGCATAATTAATTTTAATCTCCTTTTTTCA11120.21509161703633928No Hit
GAGTATGTAAATAATGCCTAGTTTTGAAGTAATGATTAACTGCATTTTCA11080.21431790618369057No Hit
TCCACAGAGTATGTAAATAATGCCTAGTTTTGAAGTAATGATTAACTGCA11080.21431790618369057No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN10860.2100624964941227No Hit
AGTCTAGACTCTGTGGTATTGTGAGGATTCTTGTCAACAAGAAAAACCCC10150.1963291288596082No Hit
TTATTAATAGATGTCAACAATATGTGGGCCCTCTTACAGTTAATGAAAAA10070.19478170715431084No Hit
CTCCAAAAGACACCAAATACTCAAGAACAGTTTCTCTTCCAAAAGTAAGA9590.1854971769225264No Hit
CCCTAGCAGCCATGGAAAGGAGGTGTATTTCCGAGAGAGGACAACAGAGT9160.17717978525655284No Hit
ATGTGGTATTGGGGGCCAAGTCTGTACAACATCTTGAGTCCCTTTTTACC9030.17466522498544457No Hit
CATTAAGATTCCCGAGATTGAGATCTTCTGCGACGCGGCGATTGAGACCT8850.17118352614852542No Hit
TTGACATACTTTCCAATCAATAGGTCTATTTACAGGCAGTTTTCGAAAAC8370.16189899591674098No Hit
TATTAATAGATGTCAACAATATGTGGGCCCTCTTACAGTTAATGAAAAAA8260.15977129107195703No Hit
GGATATTATTAATAGATGTCAACAATATGTGGGCCCTCTTACAGTTAATG8150.15764358622717312No Hit
GTATACATGCATATAAAGGCATCAAGGCAGGATAGCCACATTGTGTAAAG8080.15628959223503788No Hit
GTTTTGAAGTAATGATTAACTGCATTTTCAGGATAATACGGTTTAATGCC8000.15474217052974046No Hit
CCTCCTGCTTACAGACCACCAAATGCCCCTATCCTATCAACACTTCCGGA7960.15396845967709177No Hit
CATGTATACACTCTAAGCAGGCTTTCACTTTCTCGCCAACTTACAAGGCC7720.14932619456119955No Hit
AGTTAAGGGAGTAGCCCCAACGTTTGGTTTTATTGGGGTTCAAATGTATA7560.14623135115060476No Hit
CCTTTACACAATGTGGCTATCCTGCCTTGATGCCTTTATATGCATGTATA7500.1450707848716317No Hit
CATTTGAACCCCAATAAAACCAAACGTTGGGGCTACTCCCTTAACTTCAT7390.14294308002684777No Hit
CATTTAGATTCCCGAGATTGAGATCTTCTGCGACGCGGCGATTGAGACCT7300.14120223060838819No Hit
TGTATACACTCTAAGCAGGCTTTCACTTTCTCGCCAACTTACAAGGCCTT7280.14081537518206383No Hit
GTCTATTTACAGGCAGTTTTCGAAAACATTGCTTGATTTTTTGTACAATA7120.13772053177146903No Hit
CTATATAAGAGAGAAACTACACGCAGCGCCTCATTTTGTGGGTCACCATA7090.1371402486319825No Hit
CTGTAAGAGGGCCCACATATTGTTGACATCTATTAATAATATCCTCTTGT7050.13636653777933377No Hit
CAATAGGTCTATTTACAGGCAGTTTTCGAAAACATTGCTTGATTTTTTGT7020.13578625463984728No Hit
GTACAACATCTTGAGTCCCTTTTTACCTCTATTACCAATTTTCTTTTGTC6940.13423883293454986No Hit
GCTCTATATCGGGAGGCCTTAGAGTCTCCGGAACATTGTTCACCTCACCA6820.13191770037660375No Hit
CTATTAATAATATCCTCTTGTAAATGAATGTGAGGAAAGGAGGGAGTTTG6740.13037027867130635No Hit
CAGTTATATGGATGATGTGGTATTGGGGGCCAAGTCTGTACAACATCTTG6580.12727543526071156No Hit
GGTCTATTTACAGGCAGTTTTCGAAAACATTGCTTGATTTTTTGTACAAT6540.12650172440806284No Hit
GCTTTAGGGCATGGACATTGACCCATATAAAGAATTTGGAGCTTCTGTGG6540.12650172440806284No Hit
ATACCACATCATCCATATAACTGAAAGCCAAACAGTGGGGGAAAGCCCTA6540.12650172440806284No Hit
GTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATTGCTGAGAGTCCAA6510.12592144126857632No Hit
GTGTATACATGCATATAAAGGCATCAAGGCAGGATAGCCACATTGTGTAA6470.1251477304159276No Hit
ATTTACAGGCAGTTTTCGAAAACATTGCTTGATTTTTTGTACAATATGTT6250.12089232072635975No Hit
ATTTACATACTCTGTGGAAGGCTGGCATTCTATATAAGAGAGAAACTACA6250.12089232072635975No Hit
ATGTAAATAATGCCTAGTTTTGAAGTAATGATTAACTGCATTTTCAGGAT6150.118958043594738No Hit
CTTACAGTTAATGAAAAAAGGAGATTAAAATTAATTATGCCTGCTAGGTT6150.118958043594738No Hit
GGGTAAAGGTTCAGATATTGTTTACACAGAAAGGCCTTGTAAGTTGGCGA6120.11837776045525146No Hit
GCCTCTGGCCAGTGATCCTTGTTGGGGTTGAAGTCCCAATCTGGATTGTT6080.11760404960260278No Hit
GCACGGGACCATGCAAGACCTGCACAGTTCCTGCTCAAGGAACCTCTATG6060.11721719417627842No Hit
GGATAATACGGTTTAATGCCTTTATCCAAGGGCAAATATTTGGTAAGGTT6040.11683033874995406No Hit
CTATTACCAATTTTCTTTTGTCTTTGGGTATACATTTGAACCCCAATAAA6010.11625005561046753No Hit
CTTATATAAGACCTTGGGCAAGACCTGGCGGGCGTTCACGGTGGTCTCCA5920.11450920619200797No Hit
ATATGTTCTTGCGGTAAAGTACCCCAACTTCCAATTACATATCCCATGAA5920.11450920619200797No Hit
GTTATATGGATGATGTGGTATTGGGGGCCAAGTCTGTACAACATCTTGAG5890.11392892305252143No Hit
GGATAGAACCTAGCAGGCATAATTAATTTTAATCTCCTTTTTTCATTAAC5760.11141436278141313No Hit
CTCTAAGCAGGCTTTCACTTTCTCGCCAACTTACAAGGCCTTTCTGTGTA5750.11122093506825095No Hit
GTTTAATGCCTTTATCCAAGGGCAAATATTTGGTAAGGTTAGGATAGAAC5720.11064065192876445No Hit
CTAGTAAACTGAGCCAGGAGAAACGGACTGAGGCCCACTCCCATAGGAAT5680.10986694107611573No Hit
CCTAATCTCCTCCCCCAACTCCTCCCAGTCTTTAAACACACAGTCTTTGA5670.10967351336295356No Hit
CTCCCATAGGAATCTTGCGAAAGCCCAGGATGATGGGATGGGAATACAAG5670.10967351336295356No Hit
CAATAAAACCAAACGTTGGGGCTACTCCCTTAACTTCATGGGATATGTAA5550.10735238080500746No Hit
CTCTCAGAGGTGGAGAGATGGGAGTAGGCTGTCTCCCTGACTGCCGATTG5500.10638524223919657No Hit
CTTCCACAGAGTATGTAAATAATGCCTAGTTTTGAAGTAATGATTAACTG5450.10541810367338571No Hit
ATGTAATTGGAAGTTGGGGTACTTTACCGCAAGAACATATTGTACAAAAA5360.10367725425492612No Hit
GCCTTAGAGTCTCCGGAACATTGTTCACCTCACCATACAGCACTCAGGCA5360.10367725425492612No Hit
CTCTAAGGCCTCCCGATATAGAGCTGAGGCGGTGTCGAGGAGATCTCGAA5330.10309697111543958No Hit
ATGTTAATATGGGCCTGAAAATCAGACAACTATTGTGGTTTCACATTTCC5310.10271011568911526No Hit
GCCCAAAAGACCCACAATTCTTTGACATACTTTCCAATCAATAGGTCTAT5290.1023232602627909No Hit
TTCCCCCACTGTTTGGCTTTCAGTTATATGGATGATGTGGTATTGGGGGC5260.10174297712330437No Hit
ATTGACAACAGTGCCAGCAGCACCTCCTCCTGCCTCCACCAATCGGCAGT5200.10058241084433131No Hit
CTTTTTACCTCTATTACCAATTTTCTTTTGTCTTTGGGTATACATTTGAA5190.10038898313116913No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)