FastQCFastQC Report
Sun 4 Sep 2022
EGAF00002490253

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002490253
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences516989
Sequences flagged as poor quality0
Sequence length35-151
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATGTAAATAATGCCTAGTTTTGAAGTAATGATTAACTGCATTTTCAGG37040.7164562495526984No Hit
ATCCTAACCTTACCAAATATTTGCCCTTGGATAAAGGCATTAAACCGTAT25860.5002040662373861No Hit
CTTATATAGAATGCCAGCCTTCCACAGAGTATGTAAATAATGCCTAGTTT24900.48163500577381724No Hit
CTCTTACAGTTAATGAAAAAAGGAGATTAAAATTAATTATGCCTGCTAGG19720.3814394503558103No Hit
GTGTAAAGGGGGCAGCAAAGCCCAAAAGACCCACAATTCTTTGACATACT15970.3089040579199944No Hit
CTCTTATATAGAATGCCAGCCTTCCACAGAGTATGTAAATAATGCCTAGT14620.2827913166431007No Hit
GTTCTATCCTAACCTTACCAAATATTTGCCCTTGGATAAAGGCATTAAAC13600.2630616899005588No Hit
CCCTTAACTTCATGGGATATGTAATTGGAAGTTGGGGTACTTTACCGCAA13570.2624814067610723No Hit
GTGCTGGTGAACAGACCAATTTATGCCTACAGCCTCCTAGTACAAAGATC13380.25880628021099095No Hit
GGTTAGGATAGAACCTAGCAGGCATAATTAATTTTAATCTCCTTTTTTCA11780.22785784610504287No Hit
GAGTATGTAAATAATGCCTAGTTTTGAAGTAATGATTAACTGCATTTTCA11270.2179930327337719No Hit
ATCTTAATGTTAGTATCCCTTGGACTCATAAGGTGGGAAACTTTACTGGG11110.2148981893231771No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN10780.2085150747888253No Hit
AGTCTGTACAACATCTTGAGTCCCTTTTTACCTCTATTACCAATTTTCTT10690.20677422537036572No Hit
ATCTAAATGTTAGTATCCCTTGGACTCATAAGGTGGGAAACTTTACTGGG10640.2058070868045548No Hit
TTATTAATAGATGTCAACAATATGTGGGCCCTCTTACAGTTAATGAAAAA10640.2058070868045548No Hit
TCCACAGAGTATGTAAATAATGCCTAGTTTTGAAGTAATGATTAACTGCA10060.19458827944114865No Hit
CTCCAAAAGACACCAAATACTCAAGAACAGTTTCTCTTCCAAAAGTAAGA9920.19188029145687818No Hit
CCTCCTGCTTACAGACCACCAAATGCCCCTATCCTATCAACACTTCCGGA9860.19071972517790514No Hit
AGTCTAGACTCTGTGGTATTGTGAGGATTCTTGTCAACAAGAAAAACCCC9540.18453003835671553No Hit
GTATACATGCATATAAAGGCATCAAGGCAGGATAGCCACATTGTGTAAAG8870.17157038157484975No Hit
ATGTGGTATTGGGGGCCAAGTCTGTACAACATCTTGAGTCCCTTTTTACC8590.16615440560630884No Hit
GTCTATTTACAGGCAGTTTTCGAAAACATTGCTTGATTTTTTGTACAATA8490.1642201284746871No Hit
CCCTAGCAGCCATGGAAAGGAGGTGTATTTCCGAGAGAGGACAACAGAGT8370.16189899591674098No Hit
CATTAAGATTCCCGAGATTGAGATCTTCTGCGACGCGGCGATTGAGACCT8320.1609318573509301No Hit
GTTTTGAAGTAATGATTAACTGCATTTTCAGGATAATACGGTTTAATGCC8100.15667644766136224No Hit
GGATATTATTAATAGATGTCAACAATATGTGGGCCCTCTTACAGTTAATG7890.15261446568495654No Hit
GCTTTAGGGCATGGACATTGACCCATATAAAGAATTTGGAGCTTCTGTGG7610.14719848971641564No Hit
CATGTATACACTCTAAGCAGGCTTTCACTTTCTCGCCAACTTACAAGGCC7320.14158908603471254No Hit
CTATATAAGAGAGAAACTACACGCAGCGCCTCATTTTGTGGGTCACCATA7160.13849424262411772No Hit
CTGTAAGAGGGCCCACATATTGTTGACATCTATTAATAATATCCTCTTGT7160.13849424262411772No Hit
GTGTATACATGCATATAAAGGCATCAAGGCAGGATAGCCACATTGTGTAA7150.13830081491095556No Hit
GTACAACATCTTGAGTCCCTTTTTACCTCTATTACCAATTTTCTTTTGTC7130.1379139594846312No Hit
TATTAATAGATGTCAACAATATGTGGGCCCTCTTACAGTTAATGAAAAAA7070.13675339320565813No Hit
TTGACATACTTTCCAATCAATAGGTCTATTTACAGGCAGTTTTCGAAAAC7050.13636653777933377No Hit
CAATAGGTCTATTTACAGGCAGTTTTCGAAAACATTGCTTGATTTTTTGT7030.13597968235300945No Hit
CATTTAGATTCCCGAGATTGAGATCTTCTGCGACGCGGCGATTGAGACCT7020.13578625463984728No Hit
TGTATACACTCTAAGCAGGCTTTCACTTTCTCGCCAACTTACAAGGCCTT6890.13327169436873898No Hit
CCTTTACACAATGTGGCTATCCTGCCTTGATGCCTTTATATGCATGTATA6880.13307826665557682No Hit
GCTCTATATCGGGAGGCCTTAGAGTCTCCGGAACATTGTTCACCTCACCA6860.13269141122925246No Hit
GTTTAATGCCTTTATCCAAGGGCAAATATTTGGTAAGGTTAGGATAGAAC6840.1323045558029281No Hit
GGATAGAACCTAGCAGGCATAATTAATTTTAATCTCCTTTTTTCATTAAC6750.13056370638446854No Hit
CTATTAATAATATCCTCTTGTAAATGAATGTGAGGAAAGGAGGGAGTTTG6720.129983423244982No Hit
GTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATTGCTGAGAGTCCAA6660.12882285696600895No Hit
CATTTGAACCCCAATAAAACCAAACGTTGGGGCTACTCCCTTAACTTCAT6620.12804914611336024No Hit
CAGTTATATGGATGATGTGGTATTGGGGGCCAAGTCTGTACAACATCTTG6590.12746886297387372No Hit
AGTTAAGGGAGTAGCCCCAACGTTTGGTTTTATTGGGGTTCAAATGTATA6500.12572801355541413No Hit
GGATAATACGGTTTAATGCCTTTATCCAAGGGCAAATATTTGGTAAGGTT6480.12534115812908977No Hit
GGTCTATTTACAGGCAGTTTTCGAAAACATTGCTTGATTTTTTGTACAAT6440.12456744727644109No Hit
GTTATATGGATGATGTGGTATTGGGGGCCAAGTCTGTACAACATCTTGAG6170.11934489902106235No Hit
CTCTAAGCAGGCTTTCACTTTCTCGCCAACTTACAAGGCCTTTCTGTGTA6160.11915147130790017No Hit
CTATTACCAATTTTCTTTTGTCTTTGGGTATACATTTGAACCCCAATAAA6140.11876461588157582No Hit
GGGTAAAGGTTCAGATATTGTTTACACAGAAAGGCCTTGTAAGTTGGCGA6130.11857118816841364No Hit
ATTTACAGGCAGTTTTCGAAAACATTGCTTGATTTTTTGTACAATATGTT6110.11818433274208928No Hit
CTGCTAGGGTGTGCTGCCAACTGGATCCTGCGCGGGACGTCCTTTGTCTA6080.11760404960260278No Hit
ATATGTTCTTGCGGTAAAGTACCCCAACTTCCAATTACATATCCCATGAA6000.11605662789730536No Hit
CTTCCACAGAGTATGTAAATAATGCCTAGTTTTGAAGTAATGATTAACTG5980.11566977247098101No Hit
GCCTCTGGCCAGTGATCCTTGTTGGGGTTGAAGTCCCAATCTGGATTGTT5960.11528291704465665No Hit
CTCTCAGAGGTGGAGAGATGGGAGTAGGCTGTCTCCCTGACTGCCGATTG5930.11470263390517012No Hit
GTGTAGTTTCTCTCTTATATAGAATGCCAGCCTTCCACAGAGTATGTAAA5680.10986694107611573No Hit
CTCCCATAGGAATCTTGCGAAAGCCCAGGATGATGGGATGGGAATACAAG5630.10889980251030486No Hit
ATTTACATACTCTGTGGAAGGCTGGCATTCTATATAAGAGAGAAACTACA5630.10889980251030486No Hit
ATGTAAATAATGCCTAGTTTTGAAGTAATGATTAACTGCATTTTCAGGAT5610.1085129470839805No Hit
GCTTAGAGTGTATACATGCATATAAAGGCATCAAGGCAGGATAGCCACAT5580.10793266394449397No Hit
GCCTTAGAGTCTCCGGAACATTGTTCACCTCACCATACAGCACTCAGGCA5570.10773923623133182No Hit
ATACCACATCATCCATATAACTGAAAGCCAAACAGTGGGGGAAAGCCCTA5560.10754580851816964No Hit
CTTCATCTGCCGTTCCGGCCGACCACGGGGCGCACCTCTCTTTACGCGGT5540.10715895309184528No Hit
CAATAAAACCAAACGTTGGGGCTACTCCCTTAACTTCATGGGATATGTAA5460.10561153138654789No Hit
ATGTTAATATGGGCCTGAAAATCAGACAACTATTGTGGTTTCACATTTCC5410.104644392820737No Hit
CCTCTATGTTTCCCTCTTGTTGCTGTACAAAACCTTCGGACGGAAACTGC5410.104644392820737No Hit
GCACGGGACCATGCAAGACCTGCACAGTTCCTGCTCAAGGAACCTCTATG5380.10406410968125047No Hit
CTTACAGTTAATGAAAAAAGGAGATTAAAATTAATTATGCCTGCTAGGTT5360.10367725425492612No Hit
CTTTTTACCTCTATTACCAATTTTCTTTTGTCTTTGGGTATACATTTGAA5330.10309697111543958No Hit
TTCCCCCACTGTTTGGCTTTCAGTTATATGGATGATGTGGTATTGGGGGC5300.10251668797595308No Hit
CTCTAAGGCCTCCCGATATAGAGCTGAGGCGGTGTCGAGGAGATCTCGAA5290.1023232602627909No Hit
CTCTTTACGCGGTCTCCCCGTCTGTGCCTTCTCATCTGCCGGACCGTGTG5280.10212983254962872No Hit
ATGTAATTGGAAGTTGGGGTACTTTACCGCAAGAACATATTGTACAAAAA5260.10174297712330437No Hit
GCCCAAAAGACCCACAATTCTTTGACATACTTTCCAATCAATAGGTCTAT5200.10058241084433131No Hit
GTTTACTAGTGCCATTTGTTCAGTGGTTCGTAGGGCTTTCCCCCACTGTT5180.10019555541800695No Hit
GTTCCTGCTCAAGGAACCTCTATGTTTCCCTCTTGTTGCTGTACAAAACC5170.10000212770484478No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)