FastQCFastQC Report
Sat 3 Sep 2022
EGAF00002490254

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002490254
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences584143
Sequences flagged as poor quality0
Sequence length35-151
%GC46

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN97201.663976115437487No Hit
GTATGTAAATAATGCCTAGTTTTGAAGTAATGATTAACTGCATGTTCAGG17790.30454871495507096No Hit
CTTATATAGAATGCCAGCCTTCCACAGAGTATGTAAATAATGCCTAGTTT14850.25421857319183827No Hit
ATCCTAACCTTACCAAATATTTGCCCTTGGATAAAGGCATTAAACCTTAT14120.2417216332302193No Hit
GTGTAAAAGGGGCAGCAAAGCCCAAAAGACCCACAATTCTTTGACATACT13170.2254584921842768No Hit
GTATACATGCATATAAAGGCATCAAGGCAGGATAACCACATTGTGTAAAA12190.20868177826319925No Hit
ATATGTTCCTGTGGTAAAGTACCCCAACTTCCAATTACATATCCCATAAA11240.19241863721725674No Hit
CCTCCCGCTTATAGACCACCAAATGCCCCTATCTTATCAACACTTCCGGA11200.19173387338374337No Hit
AGCGAGAGGAGTGCGAATCCACACTCCAAAAGACACCAAATACTCAAGAA11120.19036434571671662No Hit
CTCTTACAGTTAATGAAAAAAGGAGATTAAAATTAATTATGCCTGCTAGG10540.1804352701307728No Hit
ACTTTATGGGATATGTAATTGGAAGTTGGGGTACTTTACCACAGGAACAT10390.17786740575509763No Hit
GTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATTGCCGAGAGTCCAA9520.16297379237618187No Hit
CCTAGTACAAAGACCTTTAGCCTAATCTCCTCCCCCAACTCCTCCCAGTC9260.15852282745834498No Hit
CTCTTATATAGAATGCCAGCCTTCCACAGAGTATGTAAATAATGCCTAGT9230.15800925458320994No Hit
CTCCAAAAGACACCAAATACTCAAGAACAGTTTCTCTTCCAAAAGTAAGG9190.15732449074969657No Hit
CTATTAATAATGTCCTCCTGTAAATGAATATTAGGAAAGGAGGGAGTTTG9030.1545854354156431No Hit
GGAACATATTGTACTAAAAATCAAGCAATGTTTTCGGAAACTGCCTGTAA9000.15407186254050806No Hit
CATATAAAGGCATCAAGGCAGGATAACCACATTGTGTAAAAGGGGCAGCA8960.15338709870699468No Hit
CCTAAATCTCCTCCCCCAACTCCTCCCAGTCTTTAAACAAACAGTCTTTG8890.1521887619983463No Hit
ATTTACAGGCAGTTTCCGAAAACATTGCTTGATTTTTAGTACAATATGTT8790.15047685241456288No Hit
GGGTAAAGGTGCAGATATTGTTTACACAGAAAGGCCTTGTAAGTTGGCGA8680.14859375187240112No Hit
CTTTTACACAATGTGGTTATCCTGCCTTGATGCCTTTATATGCATGTATA8600.14722422420537437No Hit
TTATTAATAGATGTCAACAATATGTGGGCCCTCTTACAGTTAATGAAAAA8370.1432868321626725No Hit
CATGTATACAAGCTAAGCAGGCTTTCACTTTCTCGCCAACTTACAAGGCC8280.1417461135372674No Hit
ATCTCAATCTCGGGAATCTCAATGTTAGTATCCCTTGGACTCATAAGGTG7990.13678157574429547No Hit
CTATTACCAATTTTCTTTTGTCTTTGGGTATACATTTGAACCCAAATAAA7820.13387132945186367No Hit
TAACCACATTGTGTAAAAGGGGCAGCAAAGCCCAAAAGACCCACAATTCT7590.1299339374091618No Hit
AAATAAAACCAAGCGTTGGGGCTACTCCCTCAACTTTATGGGATATGTAA7530.12890679165889174No Hit
CTGTAAGAGGGCCCACATATTGTTGACATCTATTAATAATGTCCTCCTGT7350.12582535440808157No Hit
ATTAACAACAGTGCCAGCAGCGCCTCCTCCTGCCTCCACCAATCGGCAGT7190.12308629907402811No Hit
CTATATAAGAGAGAAACTACACGCAGCGCTTCATTTTGTGGGTCACCATA7130.12205915332375804No Hit
GTCCAAGAGTCCTCTTATGTAAGACCTTGGGCAAGACCTGGTGGGCGTTC7070.12103200757348799No Hit
GTACAATATGTTCCTGTGGTAAAGTACCCCAACTTCCAATTACATATCCC7070.12103200757348799No Hit
ACGATGCAGGTCCCCTAGAAGAAGAACTCCCTCGCCTCGCAGACGAAGGT7030.12034724373997463No Hit
GTGGTAAAGTACCCCAACTTCCAATTACATATCCCATAAAGTTGAGGGAG6970.11932009798970458No Hit
ATGTGGTATTGGGGGCCAAGTCTGTACAACATCTTGAGTCCCTTTTTACC6970.11932009798970458No Hit
CTCTATTACCAATTTTCTTTTGTCTTTGGGTATACATTTGAACCCAAATA6960.11914890703132623No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGGAGCACCCACG6930.11863533415619121No Hit
GTGGTCTCCATGCGACGTGCAGAGGTGAAGCGAAGTGCACACGGACCGGC6930.11863533415619121No Hit
CAGTTATATGGATGATGTGGTATTGGGGGCCAAGTCTGTACAACATCTTG6790.11623866073889441No Hit
AGTCTGTACAACATCTTGAGTCCCTTTTTACCTCTATTACCAATTTTCTT6750.11555389690538105No Hit
CCATAAAGTTGAGGGAGTAGCCCCAACGCTTGGTTTTATTTGGGTTCAAA6710.11486913307186766No Hit
GGATAATAAGGTTTAATGCCTTTATCCAAGGGCAAATATTTGGTAAGGTT6670.11418436923835432No Hit
GGATAACCACATTGTGTAAAAGGGGCAGCAAAGCCCAAAAGACCCACAAT6630.11349960540484093No Hit
GTCTTACATAAGAGGACTCTTGGACTCTCGGCAATGTCAACGACCGACCT6540.11195888677943587No Hit
TCCCATGGCTGCTCGGGTGTGCTGCCAACTGGATCCTGCGCGGGACGTCC6490.11110293198754413No Hit
GCTTGTATACATGCATATAAAGGCATCAAGGCAGGATAACCACATTGTGT6430.11007578623727408No Hit
GGTTAGGATAAAACCTAGCAGGCATAATTAATTTTAATCTCCTTTTTTCA6330.10836387665349068No Hit
CATTTGAACCCAAATAAAACCAAGCGTTGGGGCTACTCCCTCAACTTTAT6310.10802149473673399No Hit
GTTATATGGATGATGTGGTATTGGGGGCCAAGTCTGTACAACATCTTGAG6300.10785030377835564No Hit
TCCACAGAGTATGTAAATAATGCCTAGTTTTGAAGTAATGATTAACTGCA6250.10699434898646394No Hit
CCTCTCGCTTATAGACCACCAAATGCCCCTATCTTATCAACACTTCCGGA6240.10682315802808559No Hit
ATGTAATTGGAAGTTGGGGTACTTTACCACAGGAACATATTGTACTAAAA6210.10630958515295055No Hit
GTTGTTAATATGCCCTGAGCCTGAGGGCTCCACCCCAAGAGACCGCCATG6120.1047688665275455No Hit
GCACGGGACCATGCAAGACCTGCACGATTCCTGCTCAAGGAACCTCTATG6080.10408410269403211No Hit
GAGTATGTAAATAATGCCTAGTTTTGAAGTAATGATTAACTGCATGTTCA6040.10339933886051875No Hit
AGTCTAGACTCTGTGGTATTGTGAGGATTCTTGTCAACAAGAAAAACCCC6040.10339933886051875No Hit
GTCTTTGGGTATACATTTGAACCCAAATAAAACCAAGCGTTGGGGCTACT5930.10151623831835697No Hit
GTTTTATCCTAACCTTACCAAATATTTGCCCTTGGATAAAGGCATTAAAC5930.10151623831835697No Hit
ATGTTAATATGGGCCTAAAAATCAGACAACTATTGTGGTTTCACATTTCC5880.10066028352646526No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)