FastQCFastQC Report
Sat 3 Sep 2022
EGAF00002490257

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002490257
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences478091
Sequences flagged as poor quality0
Sequence length35-151
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN25550.5344170879602419No Hit
GTGCTGGTGAACAGACCAATTTATGCCTACAGCCTCCTAGTACAAAGATC20060.4195853927390392No Hit
GTATTAAAACTCAAGCAATGTTTTCGGAAACTGCCTGTAAATAGACCTAT17610.36833991855107084No Hit
ATTCAAGATCTCCTCGACACCGCCTCTGCTCTGTATCGGGAGGCCTTAGA13960.29199462027103623No Hit
GTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATTGCTGAGAGTCCAA10840.22673507763166426No Hit
CCTCCCGCTTACAGACCACCAAATGCCCCTATCTTATCAACACTTCCGGA10610.22192427801401826No Hit
CATTAAGATTCCCGAGATTGAGATCTTCTGCGACGCGGCGATTGAGACCT10140.21209351357795903No Hit
GTGTAAAAGGGGCAGCAAAGCCCAAAAGACCCACAATTCTTTGACATACT9960.20832853996414907No Hit
CTGTTTGGCTTTCAGTTATATGGATGATGTGGTATTGGGGGCCAAGTCTG9160.19159532390277165No Hit
TCCCATGGCTGCTCGGATGTGCTGCCAACTGGATCCTGCGCGGGACGTCC8490.17758125545136805No Hit
GTCTTACATAAGAGGACTCTTGGACTCTCAGCAATGTCAACGACCGACCT8330.17423461223909256No Hit
GCCTAATCATCTCATGTTCATGTCCTACTGTTCAAGCCTCCAAGCTGTGC8250.1725612906329548No Hit
GTGGTCTCCATGCGACGTGCAGAGGTGAAGCGAAGTGCACACGGTCCGGC7500.15687390057541345No Hit
GTCTTTAAACAAACAGTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACA7470.15624640497311182No Hit
CCATAGGCGCATGCGTGGAACCTTTGTGGCTCCTCTGCCGATCCATACTG7450.15582807457157738No Hit
GTATACATGCATATAAAGGCATTAAGGCAGGATAGCCACATTGTGTAAAA7350.15373642256390518No Hit
ATAGAGTCCCAAACGGCCCCGAGACGGGTCGTCCGCGGGATTCAGCGCCG7280.15227226615853467No Hit
ATGTAAGACCTTGGGCAAGACCTGGTGGGCGTTCACGGTGGTCTCCATGC7220.15101727495393136No Hit
CATGTATACAAGCTAAGCAGGCTTTCACTTTCTCGCCAACTTACAAGGCC7190.1503897793516297No Hit
GCCACACAGTGGGGGAAAGCCCTGCGAACCACTGAACAAATGGCACTAGT7040.14725230134012146No Hit
GTATTGAAACTCAAGCAATGTTTTCGGAAACTGCCTGTAAATAGACCTAT7030.1470431361393542No Hit
GCACGATTCCTGCTCAAGGAACCTCTATGTTTCCCTCTTGTTGCTGTACA6810.1424415017224754No Hit
CATTTGAACCCTAATAAAACCAAACGCTGGGGCTACTCCCTTAACTTCAT6750.14118651051787212No Hit
GGTCTCTGCCAAGTGTTTGCTGACGCAACCCCCACTGGATGGGGCTTGGC6650.13909485851019993No Hit
CGCCTATGGCCTATGGCCAAGCCCCATCCAGTGGGGGTTGCGTCAGCAAA6650.13909485851019993No Hit
GGGTAAAGGTGCAGGTATTGTTGACACAGAAAGGCCTTGTAAGTTGGCGA6610.13825819770713108No Hit
GCCAAACAGTGGGGGAAAGCCCTGCGAACCACTGAACAAATGGCACTAGT6520.13637571090022613No Hit
GTCCTACTGTTCAAGCCTCCAAGCTGTGCCTTGGGTGGCTTTGGGGCATG6450.1349115544948556No Hit
CTGTGTGGCTTTCAGTTATATGGATGATGTGGTATTGGGGGCCAAGTCTG6340.13261073728641617No Hit
GCTATTCTGTGTTGGGGTGAGTTGATGAATCTGGCCACCTGGGTGGGAAG6230.1303099200779768No Hit
CTCCCATAGGAATCTTGCGAAAGCCCAGGATGATGGGATGGGAATACAAG6170.12905492887337347No Hit
GTATAAAGAATTTGGAGCTTCTGTGGAGTTACTCTCTTTTTTGCCTTCTG6090.12738160726723574No Hit
CATATAAAGGCATTAAGGCAGGATAGCCACATTGTGTAAAAGGGGCAGCA6080.12717244206646852No Hit
CTCTTTACGCGGTCTCCCCGTCTGTGCCTTCTCATCTGCCGGACCGTGTG6020.1259174508618652No Hit
GTCTACGTCCCGTCGGCGCTGAATCCCGCGGACGACCCGTCTCGGGGCCG5950.1244532944564947No Hit
GTTCATGTCCTACTGTTCAAGCCTCCAAGCTGTGCCTTGGGTGGCTTTGG5950.1244532944564947No Hit
GCTTAGCTTGTATACATGCATATAAAGGCATTAAGGCAGGATAGCCACAT5930.12403496405496024No Hit
TCCTTTGTCTACGTCCCGTCGGCGCTGAATCCCGCGGACGACCCGTCTCG5770.12068832084268476No Hit
CTGGTGAACAGACCAATTTATGCCTACAGCCTCCTAGTACAAAGATCATT5770.12068832084268476No Hit
CGGTGGTCTCCATGCGACGTGCAGAGGTGAAGCGAAGTGCACACGGTCCG5600.11713251242964205No Hit
GTTTTAATACAATATGTTCCTGTGGTAAAGTACCCCAACTTCCAATTACA5580.11671418202810761No Hit
CCCTTAACTTCATGGGATATGTAATTGGAAGTTGGGGTACTTTACCACAG5550.11608668642580597No Hit
ATTTATGCCTACAGCCTCCTAGTACAAAGATCATTAACCTAATCTCCTCC5450.11399503441813377No Hit
GAGTACAACAGAGTTGTCAGTCCCGATAAGTTTTGCTCCAGACCGGCTGC5410.11315837361506491No Hit
GTATCGGGAGGCCTTAGAGTCTCCGGAACATTGTTCACCTCACCATACAG5350.1119033824104616No Hit
CTGCCTAATCATCTCATGTTCATGTCCTACTGTTCAAGCCTCCAAGCTGT5330.11148505200892717No Hit
CCTCTATGTTTCCCTCTTGTTGCTGTACAAAACCTTCGGACGGAAACTGC5230.10939340000125498No Hit
GCTATGCCCCTATCTTATCAACACTTCCGGAAACTACTGTTGTTAGACGA5200.10876590439895333No Hit
AGTTAAGGGAGTAGCCCCAGCGTTTGGTTTTATTAGGGTTCAAATGTATA5200.10876590439895333No Hit
GTTTCAATACAATATGTTCCTGGGGTAAAGTACCCCAACTTCCAATTACA5180.10834757399741891No Hit
GTTCAAGCCTCCAAGCTGTGCCTTGGGTGGCTTTGGGGCATGGACATTGA5180.10834757399741891No Hit
CTTTGGGGCATGGACATTGACCCGTATAAAGAATTTGGAGCTTCTGTGGA5170.10813840879665168No Hit
CTTTATATGCATGTATACAAGCTAAGCAGGCTTTCACTTTCTCGCCAACT5160.10792924359588447No Hit
CTTGTATTCCCATCCCATCATCCTGGGCTTTCGCAAGATTCCTATGGGAG5150.10772007839511724No Hit
CCTATGGCCTATGGCCAAGCCCCATCCAGTGGGGGTTGCGTCAGCAAACA5080.10625592198974672No Hit
CTAGTAAACTGAGCCAGGAGAAACGGACTGAGGCCCACTCCCATAGGAAT5050.10562842638744507No Hit
CCTTTACCCCGTTGCCCGGCAACGGTCAGGTCTCTGCCAAGTGTTTGCTG5040.10541926118667784No Hit
CTGCATCTGCCGTTCCGGCCGACCACGGGGCGCACCTCTCTTTACGCGGT5010.1047917655843762No Hit
GTCCGGCAGATGAGAAGGCACAGACGGGGAGACCGCGTAAAGAGAGGTGC4960.10374593958054011No Hit
GGACTCGTGCAGGTCTTGCATGGTCCCGTGCTGGTAGTTGATGTTCCTGG4950.10353677437977288No Hit
ATATAAAAGAGAAACTACACGCAGCGCTTCATTTTGTGGGTCACCATATT4920.10290927877747123No Hit
CTTTTACACAATGTGGCTATCCTGCCTTAATGCCTTTATATGCATGTATA4910.10270011357670403No Hit
ATCCTAACCTTACCAAATATTTGCCCTTGGATAAAGGCATTAAACCTTAT4900.1024909483759368No Hit
GAACAAATGGCACTAGTAAACTGAGCCAGGAGAAACGGACTGAGGCCCAC4890.10228178317516959No Hit
CCTAATCTCCTCCCCCAACTCCTCCCAGTCTTTAAACAAACAGTCTTTGA4830.10102679197056627No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)