FastQCFastQC Report
Sat 3 Sep 2022
EGAF00002490258

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002490258
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences432475
Sequences flagged as poor quality0
Sequence length35-151
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTTTAAATGAATGTTAGGAAAGGAGGGAGTTTGCCAATCAGGATTAAAG15080.34869067576160473No Hit
GTGGCTATCCTGCCTTGATGCCTTTATATGCATGTATACAATCTAAGCAG14750.3410601768888375No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN12330.28510318515521127No Hit
TCTGCGGAGTTACTCTCTTTTTTGCCTTCTGACTTCTTTCCTTCTATTCG11530.266605006069715No Hit
GTACCGGGAGGCCTTAGAGTCTCCGGAACATTGTTCACCTCACCATACAG11140.2575871437655356No Hit
CTACTGTACCTGTCTTTAATCCTGATTGGCAAACTCCCTCCTTTCCTAAC11030.25504364414127983No Hit
AAGATGCAGGTCCCCTAGAAGAAGAACTCCCTCGCCTCGCAGACGAAGGT10370.2397826463957454No Hit
GTGCTGGTGAACAGACCAATTTATGCCTACAGCCTCCTAGTACAATTAAC10370.2397826463957454No Hit
GGATAACCACATTGTGTAAAAGGGGCAGCAAAGCCCAAAAGACCCACAAT10030.23192092028440947No Hit
CCCTCATAAAACCAAACGTTGGGGCTACTCCCTTAACTTCATGGGATATG9820.22706514827446675No Hit
CCTACCGCTTACAGACCACCAAATGCCCCTATCTTATCAACACTTCCGGA9730.2249841031273484No Hit
GCTTAGTGGAATGTTGTGGAGTTCCACTGCATGGCCTGAGGATGACTGTC9020.20856696918897044No Hit
CATTTACAGGAGGACATTATTGATAGATGTCAACAATATGTGGGCCCTCT8870.20509856061043993No Hit
CATGTATACAATCTAAGCAGGCTTTCACTTTCTCGCCAACTTACAAGGCC8010.18521301809353144No Hit
GTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATCGCTGAGAGTCCAA7640.17665761026648938No Hit
CTTTAGGGCATGGACATTGACCCGTATAAAGAATTTGGAGCTTCTGCGGA7500.17342042892652754No Hit
CCTGTAAATGAATGTTAGGAAAGGAGGGAGTTTGCCAATCAGGATTAAAG7250.16763974796230996No Hit
CCAACTCCCCCAGTCTTTAAACAAACAGTCTTTGAAGTATGCCTCAAGGT6980.16139661252095497No Hit
CCTAATACAATTAACCATTAACCTAATCTCCTCCCCCAAAACCCCCAGTC6770.1565408405110122No Hit
GTACCGTAGAAGAATAAAGCCCAGTAAAGTTTCCCACCTTATGAGTCCAA6730.15561593155673736No Hit
ATCTTTCTGTTCCCAATCCTCTGGGATTCTTTCCCGATCACCAGTTGGAC6670.15422856812532518No Hit
CATTAGGATTCCCGAGATTGAGATCTTCTGCGACGCGGCGATTGAGACCT6610.15284120469391294No Hit
GACCAGACCGTTGCCGGGCAACGGGGTAAAGGTGCAGGTATTGTTGACAC6330.14636684201398925No Hit
ATCCTAACCTTACCAAATATTTGCCCTTGGATAAAGGCATTAAACCTTAT6290.14544193305971442No Hit
TCTCATGTTCATGTCCTACTGTTCAAGCCTCCAAGCTGTGCCTTGGGTGG6230.1440545696283022No Hit
CAGTTCATCATTACTTCAAAACTAGGCATTATTTACATACTCTGTGGAAG6170.14266720619689No Hit
ATACTACCATTGAGAGTCCCGAGATTGAGATCTTCTGCGACGCGGCGATT6120.14151107000404647No Hit
TTATTAGGGTTCAAATGTATACCCAAAGACAAAAGAAAATTGGTAATAGA5790.13388057113127927No Hit
GTGTAAAAGGGGCAGCAAAGCCCAAAAGACCCACAATTCTTTGACATACT5740.13272443493843575No Hit
GGTAAAAAGGGACTCAAGATGTTGTACAGACTTGGCCCCCAATACCACAT5730.13249320769986705No Hit
GTGTACACTTCGCTTCACCTCTGCACGTCGCATGGAAACCACCGTGAACG5700.13179952598416095No Hit
GTGGTTATCCTGCCTTGATGCCTTTATATGCATGTATACAATCTAAGCAG5580.1290247991213365No Hit
CCAACTTGTCCTGGCTATCGCTGGATGTGTCTGCGGCGTTTTATCATATT5460.12625007225851206No Hit
TGATTAACTGCATGTTCAGGATAATAAGGTTTAATGCCTTTATCCAAGGG5400.12486270882709984No Hit
AGTCTAGACTCTGTGGTATTGTGAGGATTCTTGTCAACAAGAAAAACCCC5380.12440025434996244No Hit
CCTCCCGCTTACAGACCACCAAATGCCCCTATCTTATCAACACTTCCGGA5330.1232441181571189No Hit
CCTGCACCCCTGGCCCGAATGCTCCCGCTCCTACCTGATTTGCCTCTGGC5280.12208798196427538No Hit
GTATTAAAACTTAAGCAATGTTTTCGGAAACTGCCTGTAAATAGACCTAT5040.11653852823862651No Hit
CCAACTCCTCCCAGTCTTTAAACAAACAGTCTTTGAAGTATGCCTCAAGG4990.115382392045783No Hit
ACATTGTTCACCTCACCATACAGCACTCAGGCAAGCTATTCTGTGTTGGG4950.11445748309150817No Hit
GATAACCACATTGTGTAAAAGGGGCAGCAAAGCCCAAAAGACCCACAATT4900.11330134689866465No Hit
TTCCCCCACTGTCTGGCTTTCAGTTATATGGATGATGTGGTATTGGGGGC4870.11260766518295855No Hit
CCCTTAACTTCATGGGATATGTAATTGGAAGTTGGGGTACTTTACCACAG4870.11260766518295855No Hit
CTGTAAGAGGGCCCACATATTGTTGACATCTATCAATAATGTCCTCCTGT4760.11006416555870281No Hit
TCCCATGGCTGCTCGGGTGTGCTGCCAACTGGATCCTGCGCGGGACGTCC4760.11006416555870281No Hit
GGATAGCCACATTGTGTAAAAGGGGCAGCAAAGCCCAAAAGACCCACAAT4600.10636452974160356No Hit
AGTGCACACGGTCCGGCAGATGAGAAGGCACAGACGGGGAGACCGCGTAA4600.10636452974160356No Hit
CATAGGAATCTTGCGAAAGCCCAGGATGATGGGATGGGAATACAAGTGCA4530.10474593907162265No Hit
TCTCTTGTTCATGTCCTACTGTTCAAGCCTCCAAGCTGTGCCTTGGGTGG4510.10428348459448522No Hit
GACCGGCTGCGAGCAAAACAAGCTGCTAGGAGTTCCGCAGTATGGATCGG4460.10312734840164171No Hit
CTGTACCGGGAGGCCTTAGAGTCTCCGGAACATTGTTCACCTCACCATAC4430.10243366668593559No Hit
GTACAGTAGAAGAATAAAGCCCAGTAAAGTTTCCCACCTTATGAGTCCAA4340.10035262153881729No Hit
GGTCTCTGCCAAGTGTTTGCTGACGCAACCCCCACTGGATGGGGCTTGGC4330.10012139430024856No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)