FastQCFastQC Report
Sat 3 Sep 2022
EGAF00002490259

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002490259
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences432475
Sequences flagged as poor quality0
Sequence length35-151
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTTTAAATGAATGTTAGGAAAGGAGGGAGTTTGCCAATCAGGATTAAAG15770.36464535522284525No Hit
GTGGCTATCCTGCCTTGATGCCTTTATATGCATGTATACAATCTAAGCAG14360.33204231458465805No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN12340.28533441239378No Hit
TCTGCGGAGTTACTCTCTTTTTTGCCTTCTGACTTCTTTCCTTCTATTCG11550.26706746054685243No Hit
CTACTGTACCTGTCTTTAATCCTGATTGGCAAACTCCCTCCTTTCCTAAC11550.26706746054685243No Hit
GTACCGGGAGGCCTTAGAGTCTCCGGAACATTGTTCACCTCACCATACAG10630.2457945545985317No Hit
CCCTCATAAAACCAAACGTTGGGGCTACTCCCTTAACTTCATGGGATATG10240.23677669229435228No Hit
GCTTAGTGGAATGTTGTGGAGTTCCACTGCATGGCCTGAGGATGACTGTC9680.22382796693450488No Hit
GTGCTGGTGAACAGACCAATTTATGCCTACAGCCTCCTAGTACAATTAAC9560.22105324007168048No Hit
AAGATGCAGGTCCCCTAGAAGAAGAACTCCCTCGCCTCGCAGACGAAGGT9210.2129602867217758No Hit
GGATAACCACATTGTGTAAAAGGGGCAGCAAAGCCCAAAAGACCCACAAT9180.2122666050060697No Hit
CATTTACAGGAGGACATTATTGATAGATGTCAACAATATGTGGGCCCTCT8890.2055610150875773No Hit
CCTACCGCTTACAGACCACCAAATGCCCCTATCTTATCAACACTTCCGGA8520.19700560726053526No Hit
CTTTAGGGCATGGACATTGACCCGTATAAAGAATTTGGAGCTTCTGCGGA8160.18868142667206197No Hit
CATGTATACAATCTAAGCAGGCTTTCACTTTCTCGCCAACTTACAAGGCC8040.18590669980923755No Hit
GTACCGTAGAAGAATAAAGCCCAGTAAAGTTTCCCACCTTATGAGTCCAA7550.17457656511937106No Hit
CCTGTAAATGAATGTTAGGAAAGGAGGGAGTTTGCCAATCAGGATTAAAG7240.16740852072374127No Hit
TCCCATGGCTGCTCGGGTGTGCTGCCAACTGGATCCTGCGCGGGACGTCC7110.1644025666223481No Hit
GTGTACACTTCGCTTCACCTCTGCACGTCGCATGGAAACCACCGTGAACG6820.1576969767038557No Hit
ATCTTTCTGTTCCCAATCCTCTGGGATTCTTTCCCGATCACCAGTTGGAC6650.15376611364818776No Hit
GTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATCGCTGAGAGTCCAA6510.1505289323082259No Hit
TCTCATGTTCATGTCCTACTGTTCAAGCCTCCAAGCTGTGCCTTGGGTGG6480.1498352505925198No Hit
GTGTAAAAGGGGCAGCAAAGCCCAAAAGACCCACAATTCTTTGACATACT6470.1496040233539511No Hit
CATTAGGATTCCCGAGATTGAGATCTTCTGCGACGCGGCGATTGAGACCT6310.14590438753685184No Hit
ATACTACCATTGAGAGTCCCGAGATTGAGATCTTCTGCGACGCGGCGATT6150.14220475171975258No Hit
CCTAATACAATTAACCATTAACCTAATCTCCTCCCCCAAAACCCCCAGTC6080.14058616104977167No Hit
GACCAGACCGTTGCCGGGCAACGGGGTAAAGGTGCAGGTATTGTTGACAC5940.1373489797098098No Hit
ATCCTAACCTTACCAAATATTTGCCCTTGGATAAAGGCATTAAACCTTAT5930.1371177524712411No Hit
CCTCCCGCTTACAGACCACCAAATGCCCCTATCTTATCAACACTTCCGGA5890.1361928435169663No Hit
TTATTAGGGTTCAAATGTATACCCAAAGACAAAAGAAAATTGGTAATAGA5830.13480548008555407No Hit
CAGTTCATCATTACTTCAAAACTAGGCATTATTTACATACTCTGTGGAAG5720.13226198046129833No Hit
GTGGTTATCCTGCCTTGATGCCTTTATATGCATGTATACAATCTAAGCAG5580.1290247991213365No Hit
CCAACTTGTCCTGGCTATCGCTGGATGTGTCTGCGGCGTTTTATCATATT5570.1287935718827678No Hit
GGTAAAAAGGGACTCAAGATGTTGTACAGACTTGGCCCCCAATACCACAT5400.12486270882709984No Hit
CCCTTAACTTCATGGGATATGTAATTGGAAGTTGGGGTACTTTACCACAG5250.12139430024856927No Hit
CCAACTCCCCCAGTCTTTAAACAAACAGTCTTTGAAGTATGCCTCAAGGT5160.11931325510145097No Hit
GTATTAAAACTTAAGCAATGTTTTCGGAAACTGCCTGTAAATAGACCTAT5140.11885080062431354No Hit
GGTCTCTGCCAAGTGTTTGCTGACGCAACCCCCACTGGATGGGGCTTGGC5130.11861957338574484No Hit
CTGTAAGAGGGCCCACATATTGTTGACATCTATCAATAATGTCCTCCTGT5050.11676975547719522No Hit
AGTCTAGACTCTGTGGTATTGTGAGGATTCTTGTCAACAAGAAAAACCCC5030.1163073010000578No Hit
TGATTAACTGCATGTTCAGGATAATAAGGTTTAATGCCTTTATCCAAGGG4790.11075784727440892No Hit
GGTTAGGATAGAACCTAGCAGGCATAATTAATTTTAATCTCCTTTTTTCA4770.11029539279727152No Hit
TCTGTGGAGTTACTCTCTTTTTTGCCTTCTGACTTCTTTCCTTCTATTCG4760.11006416555870281No Hit
ACATTGTTCACCTCACCATACAGCACTCAGGCAAGCTATTCTGTGTTGGG4690.10844557488872189No Hit
CCTGCACCCCTGGCCCGAATGCTCCCGCTCCTACCTGATTTGCCTCTGGC4640.10728943869587838No Hit
TTCCCCCACTGTCTGGCTTTCAGTTATATGGATGATGTGGTATTGGGGGC4610.10659575698017228No Hit
ATATGTTCCTGTGGTAAAGTACCCCAACTTCCAATTACATATCCCATGAA4510.10428348459448522No Hit
GCTTTAGGGCATGGACATTGACCCGTATAAAGAATTTGGAGCTTCTGCGG4390.10150875773166079No Hit
CCAACTCCTCCCAGTCTTTAAACAAACAGTCTTTGAAGTATGCCTCAAGG4340.10035262153881729No Hit
TCTCTTGTTCATGTCCTACTGTTCAAGCCTCCAAGCTGTGCCTTGGGTGG4330.10012139430024856No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)