FastQCFastQC Report
Sat 3 Sep 2022
EGAF00002490260

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002490260
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences450830
Sequences flagged as poor quality0
Sequence length35-151
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN18840.4178958809307277No Hit
CCTATTCTCCTCCCCCAACTCCTCCCAGTCTTTAAACAAACAGTCTTTGA12860.2852516469622696No Hit
ATTCAAGATCTCCTCGACACCGCCTCTGCTCTGTATCGGGAGGCCTTAGA10790.23933633520395714No Hit
GTGCTGGTGAACAGACCAATTTATGCCTACAGCCTCCTAGTACAAAGATC10480.23246012909522434No Hit
CCTCCCGCTTACAGACCACCAAATGCCCCTATCTTATCAACACTTCCGGA9970.2211476609808575No Hit
GGTAATCTCCTCCCCCAACTCCTCCCAGTCTTTAAACAAACAGTCTTTGA9850.21848590377747712No Hit
ATGTAAGACCTTGGGCAAGACCTGGTGGGCGTTCACGGTGGTTTCCATGC9610.21316238937071624No Hit
ATCCTAACCTTACCAAATATTTGCCCTTGGATAAAGGCATTAAACCTTAT9370.20783887496395537No Hit
GTGTAAAAGGGGCAGCAAAGCCCAAAAGACCCACAATTCTTTGACATACT8990.19940997715325068No Hit
GTATACATGCATATAAAGGCATTAAGGCAGGATAGCCACATTGTGTAAAA8840.19608278064902512No Hit
CCTCTAGAAGATTGACGATATGGGTGAGGCAGTAGTCGGAACAGGGTTTA8610.19098107934254596No Hit
GTATCGGGAGGCCTTAGAGTCTCCGGAACATTGTTCACCTCACCATACAG8260.1832176208326864No Hit
CATGTATACAATCTAGGCAGGCTTTCACTTTCTCGCCAACTTACAAGGCC8020.1778941064259255No Hit
ATATAAAAGAGAAACTACACGCAGCGCTTCATTTTGTGGGTCACCATATT7720.17123971341747443No Hit
AGTCTAGACTCTGTGGTATTGTGAGGATTCTTGTCAACAAGAAAAACCCC7690.17057427411662934No Hit
ATTGACAACAGTGCCAGCGGCGCCTCCTCCTGCCTCCACCAATCGGCAGT7680.17035246101634763No Hit
GTTGTCAATATGCCCTGAGCCTGAGGGCTCCACCCCAAAAGACCGCCGTG7680.17035246101634763No Hit
CCTAATCTCCTCCCCCAACTCCTCCCAGTCTTTAAACAAACAGTCTTTGA7590.1683561431138123No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGGAGCACCCACG7440.16502894660958678No Hit
TCTGTGGAGTTACTCTCTTTTTTGCCTTCTGACTTCTTTCCTTCTATTCG7410.16436350730874166No Hit
GTATGTAAATAATGCCTAGTTTTGAAGTAATGATTAACTGCATGTTCAGG7360.16325444180733314No Hit
CCTACCGCTTACAGACCACCAAATGCCCCTATCTTATCAACACTTCCGGA7260.1610363108045161No Hit
GTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATTGCTGAGAGTCCAA7170.1590399929019808No Hit
CTATTAATAATGTCCTCCTGTAAATGAATGGTAGGAAAGGAGGGAGTTTG6940.15393829159550163No Hit
CTGTAAGAGGGCCCACATATTGTTGACATCTATTAATAATGTCCTCCTGT6900.15305103919437482No Hit
CTACCGTCCCCTTCTGCGTCTGCCGTTCCGACCGACCACGGGGCGCACCT6820.1512765343921212No Hit
TCCACTTTACTTCCCACCCAGGTGGCCAGATTCATCAACTCACCCCAACA6680.14817115098817737No Hit
GTTAATATGGGCCTAAAAATCAGACAACTATTGTGGTTTCACATTTCCTG6550.14528758068451522No Hit
ATCTAGGCAGGCTTTCACTTTCTCGCCAACTTACAAGGCCTTTCTGTGTC6510.14440032828338842No Hit
CCCTTGGACTCATAAGGTGGGAAACTTTACTGGGCTTTATTCTTCTACTG6480.1437348889825433No Hit
GGTTAGGATAGAACCTAGCAGGCATAATTAATTTTAATCTCCTTTTTTCA6480.1437348889825433No Hit
CTCCTCCCGCTTACAGACCACCAAATGCCCCTATCTTATCAACACTTCCG6470.14351307588226161No Hit
GCTGAGAGTCCAAGAGTCCTCTTATGTAAGACCTTGGGCAAGACCTGGTG6460.1432912627819799No Hit
GCGGTAGGAGTGCGAATCCACACTCCAAAAGACACCAAATACTCAAGAAC6360.1410731317791629No Hit
CCCTTAACTTCATGGGATATGTAATTGGAAGTTGGGGTACTTTACCACAG6330.14040769247831777No Hit
ATACCACATCATCCATATAACTGAAAGCCAAACAGTGGGGGAAAGCCCTG6190.13730230907437393No Hit
GTCCAAGGGATACTAACATTGAGATTCCCGAGATTGAGATCTTCTGCGAC6110.13552780427212033No Hit
CTCCTCGACACCGCCTCTGCTCTGTATCGGGAGGCCTTAGAGTCTCCGGA6030.1337532994698667No Hit
CTACAAGTTCCCTGGATGCTGGGTCTTCCAAATTACTTCCCACCCAGGTG5970.13242242086817646No Hit
GCCTTAGAGTCTCCGGAACATTGTTCACCTCACCATACAGCACTCAGGCA5950.13197879466761306No Hit
GTCCGGCAGATGAGAAGGCACAGACGGGGAGACCGCGTAAAGAGAGGTGC5910.13109154226648626No Hit
CTCTTACAGTTAATGAAAAAAGGAGATTAAAATTAATTATGCCTGCTAGG5870.13020428986535945No Hit
CATTTGAACCCTAATAAAACCAAACGCTGGGGCTACTCCCTTAACTTCAT5870.13020428986535945No Hit
ATCTAAGCAGGCTTTCACTTTCTCGCCAACTTACAAGGCCTTTCTGTGTC5660.1255462147594437No Hit
ATTTACATACCCTGTGGAAGGCTGGCATTCTATATAAAAGAGAAACTACA5620.12465896235831689No Hit
GCACGGGACCATGCAAGACCTGCACGATTCCTGCTCAAGGAACCTCTATG5540.12288445755606325No Hit
TCCCATGGCTGCTCGGGTGTGCTGCCAACTGGATCCTGCGCGGGACGTCC5540.12288445755606325No Hit
CTCCCATAGGAATCTTGCGAAAGCCCAGGATGATGGGATGGGAATACAAG5500.12199720515493645No Hit
ATACAGCACTCAGGCAAGCTATTCTGTGTTGGGGTGAGTTGATGAATCTG5450.12088813965352795No Hit
GTATTAAAACTCAAGCAATGTTTTCGGAAACTGCCTGTAAATAGACCTAT5430.12044451345296454No Hit
ATATGTTCCTGTGGTAAAGTACCCCAACTTCCAATTACATATCCCATGAA5410.12000088725240111No Hit
CCTATGGCCTATGGCCAAGCCCCATCCAGTGGGGGTTGCGTCAGCAAACA5380.11933544795155603No Hit
ACCACAGAGTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGGAG5330.11822638245014752No Hit
CTACTAGTTCCCTGGATGCTGGGTCTTCCAAATTACTTCCCACCCAGGTG5320.1180045693498658No Hit
AACTAGTAGTCAGCTATGTCAATGTTAATATGGGCCTAAAAATCAGACAA5310.11778275624958409No Hit
CCTTTACCCCGTTGCCCGGCAACGGTCAGGTCTCTGCCAAGTGTTTGCTG5290.11733913004902069No Hit
GTATAAAGAATTTGGAGCTTCTGTGGAGTTACTCTCTTTTTTGCCTTCTG5290.11733913004902069No Hit
GGATAGCCACATTGTGTAAAAGGGGCAGCAAAGCCCAAAAGACCCACAAT5280.117117316948739No Hit
TTCTCGAGGACTGGGGACCCTGCACCGAACATGGAGAGCACAACATCAGG5270.11689550384845729No Hit
CTCCAAAAGACACCAAATACTCAAGAACAGTTTCTCTTCCAAAAGTAAGA5240.11623006454761217No Hit
TTGGAAGACCAACCTCCCATGCTGTAGCTCTTGTTCCCAAGAATATGGTG5040.11179380254197813No Hit
CCCCTGGACTCATAAGGTGGGAAACTTTACTGGGCTTTATTCTTCTACTG5010.11112836324113301No Hit
AGTTAAGGGAGTAGCCCCAGCGTTTGGTTTTATTAGGGTTCAAATGTATA5000.11090655014085132No Hit
GGGTAAAGGTGCAGGTATTGTTGACACAGAAAGGCCTTGTAAGTTGGCGA4970.1102411108400062No Hit
CACATATTGTTGACATCTATTAATAATGTCCTCCTGTAAATGAATGGTAG4930.1093538584388794No Hit
CTTTTACACAATGTGGCTATCCTGCCTTAATGCCTTTATATGCATGTATA4860.10780116673690747No Hit
CTCCTACCGCTTACAGACCACCAAATGCCCCTATCTTATCAACACTTCCG4860.10780116673690747No Hit
GGATTAAAGACAGGTACAGTAGAAGAATAAAGCCCAGTAAAGTTTCCCAC4840.10735754053634407No Hit
CTAGTAAACTGAGCCAGGAGAAACGGACTGAGGCCCACTCCCATAGGAAT4840.10735754053634407No Hit
GCCTAATCATCTCATGTTCATGTCCTACTGTTCAAGCCTCCAAGCTGTGC4800.10647028813521728No Hit
CTTTTATATAGAATGCCAGCCTTCCACAGGGTATGTAAATAATGCCTAGT4760.10558303573409045No Hit
CCTCTATGTTTCCCTCTTGTTGCTGTACAAAACCTTCGGACGGAAACTGC4720.10469578333296364No Hit
ACATAGAGGTTCCTTGAGCAGGAATCGTGCAGGTCTTGCATGGTCCCGTG4710.10447397023268193No Hit
CTCTTTACGCGGTCTCCCCGTCTGTGCCTTCTCATCTGCCGGACCGTGTG4710.10447397023268193No Hit
GGCTTTCACTTTCTCGCCAACTTACAAGGCCTTTCTGTGTCAACAATACC4700.10425215713240024No Hit
GTCTTACATAAGAGGACTCTTGGACTCTCAGCAATGTCAACGACCGACCT4650.10314309163099174No Hit
GTCTTTAAACAAACAGTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACA4650.10314309163099174No Hit
GTACAGACTTGGCCCCCAATACCACATCATCCATATAACTGAAAGCCAAA4620.10247765233014662No Hit
TTTACATACCCTGTGGAAGGCTGGCATTCTATATAAAAGAGAAACTACAC4600.10203402612958322No Hit
GCCATGGGAAGGAGGTGTATTTCCGAGAGAGGACAACAGAGTTGTCAGTC4590.1018122130293015No Hit
ATTAAAACTCAAGCAATGTTTTCGGAAACTGCCTGTAAATAGACCTATTG4570.1013685868287381No Hit
GTTCAAGCCTCCAAGCTGTGCCTTGGGTGGCTTTGGGGCATGGACATTGA4530.10048133442761129No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)