FastQCFastQC Report
Sat 3 Sep 2022
EGAF00002490261

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002490261
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences450830
Sequences flagged as poor quality0
Sequence length35-151
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN18720.41523412372734736No Hit
CCTCCCGCTTACAGACCACCAAATGCCCCTATCTTATCAACACTTCCGGA10340.22935474569128053No Hit
ATTCAAGATCTCCTCGACACCGCCTCTGCTCTGTATCGGGAGGCCTTAGA9860.2187077168777588No Hit
GTGCTGGTGAACAGACCAATTTATGCCTACAGCCTCCTAGTACAAAGATC9720.21560233347381494No Hit
ATCCTAACCTTACCAAATATTTGCCCTTGGATAAAGGCATTAAACCTTAT9670.21449326797240645No Hit
CTACCGTCCCCTTCTGCGTCTGCCGTTCCGACCGACCACGGGGCGCACCT9620.21338420247099793No Hit
CCTATTCTCCTCCCCCAACTCCTCCCAGTCTTTAAACAAACAGTCTTTGA9340.20717343566311028No Hit
GTGTAAAAGGGGCAGCAAAGCCCAAAAGACCCACAATTCTTTGACATACT9040.2005190426546592No Hit
GTATACATGCATATAAAGGCATTAAGGCAGGATAGCCACATTGTGTAAAA8700.19297739724508128No Hit
GTATCGGGAGGCCTTAGAGTCTCCGGAACATTGTTCACCTCACCATACAG8580.19031564004170087No Hit
CCTCTAGAAGATTGACGATATGGGTGAGGCAGTAGTCGGAACAGGGTTTA8450.18743206973803872No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGGAGCACCCACG8140.18055586362930595No Hit
TCCCATGGCTGCTCGGGTGTGCTGCCAACTGGATCCTGCGCGGGACGTCC7740.17168333961803786No Hit
GTATGTAAATAATGCCTAGTTTTGAAGTAATGATTAACTGCATGTTCAGG7710.17101790031719274No Hit
GTTGTCAATATGCCCTGAGCCTGAGGGCTCCACCCCAAAAGACCGCCGTG7700.17079608721691103No Hit
ATATAAAAGAGAAACTACACGCAGCGCTTCATTTTGTGGGTCACCATATT7660.16990883481578423No Hit
CATGTATACAATCTAGGCAGGCTTTCACTTTCTCGCCAACTTACAAGGCC7520.1668034514118404No Hit
ATTGACAACAGTGCCAGCGGCGCCTCCTCCTGCCTCCACCAATCGGCAGT7420.16458532040902335No Hit
AGTCTAGACTCTGTGGTATTGTGAGGATTCTTGTCAACAAGAAAAACCCC7090.15726548809972715No Hit
GGTTAGGATAGAACCTAGCAGGCATAATTAATTTTAATCTCCTTTTTTCA6860.15216378679324802No Hit
TCTGTGGAGTTACTCTCTTTTTTGCCTTCTGACTTCTTTCCTTCTATTCG6840.1517201605926846No Hit
CTGTAAGAGGGCCCACATATTGTTGACATCTATTAATAATGTCCTCCTGT6810.1510547212918395No Hit
CCTACCGCTTACAGACCACCAAATGCCCCTATCTTATCAACACTTCCGGA6730.1492802164895859No Hit
CTATTAATAATGTCCTCCTGTAAATGAATGGTAGGAAAGGAGGGAGTTTG6730.1492802164895859No Hit
CTCCTCCCGCTTACAGACCACCAAATGCCCCTATCTTATCAACACTTCCG6710.1488365902890225No Hit
CCCTTAACTTCATGGGATATGTAATTGGAAGTTGGGGTACTTTACCACAG6680.14817115098817737No Hit
CTCCTCGACACCGCCTCTGCTCTGTATCGGGAGGCCTTAGAGTCTCCGGA6650.14750571168733226No Hit
ATCTAGGCAGGCTTTCACTTTCTCGCCAACTTACAAGGCCTTTCTGTGTC6620.14684027238648714No Hit
ATGTAAGACCTTGGGCAAGACCTGGTGGGCGTTCACGGTGGTTTCCATGC6610.14661845928620545No Hit
CCCTTGGACTCATAAGGTGGGAAACTTTACTGGGCTTTATTCTTCTACTG6610.14661845928620545No Hit
GTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATTGCTGAGAGTCCAA6330.14040769247831777No Hit
CTCCAAAAGACACCAAATACTCAAGAACAGTTTCTCTTCCAAAAGTAAGA6320.14018587937803606No Hit
TCCACTTTACTTCCCACCCAGGTGGCCAGATTCATCAACTCACCCCAACA6190.13730230907437393No Hit
CTCTTACAGTTAATGAAAAAAGGAGATTAAAATTAATTATGCCTGCTAGG6010.1333096732693033No Hit
GGTAATCTCCTCCCCCAACTCCTCCCAGTCTTTAAACAAACAGTCTTTGA5910.13109154226648626No Hit
ATCTAAGCAGGCTTTCACTTTCTCGCCAACTTACAAGGCCTTTCTGTGTC5810.12887341126366922No Hit
CTCCTACCGCTTACAGACCACCAAATGCCCCTATCTTATCAACACTTCCG5760.1277643457622607No Hit
CTCTTTACGCGGTCTCCCCGTCTGTGCCTTCTCATCTGCCGGACCGTGTG5710.1266552802608522No Hit
GCCTTAGAGTCTCCGGAACATTGTTCACCTCACCATACAGCACTCAGGCA5670.1257680278597254No Hit
CCCCTGGACTCATAAGGTGGGAAACTTTACTGGGCTTTATTCTTCTACTG5670.1257680278597254No Hit
GCGGTAGGAGTGCGAATCCACACTCCAAAAGACACCAAATACTCAAGAAC5640.1251025885588803No Hit
ATTTACATACCCTGTGGAAGGCTGGCATTCTATATAAAAGAGAAACTACA5600.12421533615775349No Hit
GTTAATATGGGCCTAAAAATCAGACAACTATTGTGGTTTCACATTTCCTG5570.12354989685690837No Hit
GTATTAAAACTCAAGCAATGTTTTCGGAAACTGCCTGTAAATAGACCTAT5560.12332808375662666No Hit
ATATGTTCCTGTGGTAAAGTACCCCAACTTCCAATTACATATCCCATGAA5480.12155357895437305No Hit
CTCCCATAGGAATCTTGCGAAAGCCCAGGATGATGGGATGGGAATACAAG5400.11977907415211943No Hit
GTCCAAGGGATACTAACATTGAGATTCCCGAGATTGAGATCTTCTGCGAC5390.11955726105183771No Hit
CCTCTATGTTTCCCTCTTGTTGCTGTACAAAACCTTCGGACGGAAACTGC5360.11889182175099261No Hit
CTTTTATATAGAATGCCAGCCTTCCACAGGGTATGTAAATAATGCCTAGT5340.11844819555042921No Hit
CCTTTACCCCGTTGCCCGGCAACGGTCAGGTCTCTGCCAAGTGTTTGCTG5280.117117316948739No Hit
GGGTAAAGGTGCAGGTATTGTTGACACAGAAAGGCCTTGTAAGTTGGCGA5230.11600825144733048No Hit
ACCACAGAGTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGGAG5220.11578643834704877No Hit
CCTATGGCCTATGGCCAAGCCCCATCCAGTGGGGGTTGCGTCAGCAAACA5190.11512099904620367No Hit
CATTTGAACCCTAATAAAACCAAACGCTGGGGCTACTCCCTTAACTTCAT5160.11445555974535855No Hit
GTATAAAGAATTTGGAGCTTCTGTGGAGTTACTCTCTTTTTTGCCTTCTG5160.11445555974535855No Hit
GGATAGCCACATTGTGTAAAAGGGGCAGCAAAGCCCAAAAGACCCACAAT5130.11379012044451346No Hit
CTACTGTACCTGTCTTTAATCCTGAGTGGCAAACTCCCTCCTTTCCTACC5120.11356830734423175No Hit
ATACCACATCATCCATATAACTGAAAGCCAAACAGTGGGGGAAAGCCCTG5120.11356830734423175No Hit
AACTAGTAGTCAGCTATGTCAATGTTAATATGGGCCTAAAAATCAGACAA5050.11201561564225984No Hit
CTACTAGTTCCCTGGATGCTGGGTCTTCCAAATTACTTCCCACCCAGGTG4990.11068473704056961No Hit
GCACGGGACCATGCAAGACCTGCACGATTCCTGCTCAAGGAACCTCTATG4910.10891023223831599No Hit
TTCTCGAGGACTGGGGACCCTGCACCGAACATGGAGAGCACAACATCAGG4900.1086884191380343No Hit
GTCTACGTCCCGTCGGCGCTGAATCCCGCGGACGACCCGTCTCGGGGCCG4850.10757935363662578No Hit
GTCTTACATAAGAGGACTCTTGGACTCTCAGCAATGTCAACGACCGACCT4840.10735754053634407No Hit
TCCTTTGTCTACGTCCCGTCGGCGCTGAATCCCGCGGACGACCCGTCTCG4820.10691391433578068No Hit
ATACAGCACTCAGGCAAGCTATTCTGTGTTGGGGTGAGTTGATGAATCTG4800.10647028813521728No Hit
GTTCTATCCTAACCTTACCAAATATTTGCCCTTGGATAAAGGCATTAAAC4800.10647028813521728No Hit
CTTTTACACAATGTGGCTATCCTGCCTTAATGCCTTTATATGCATGTATA4770.10580484883437216No Hit
GGATTAAAGACAGGTACAGTAGAAGAATAAAGCCCAGTAAAGTTTCCCAC4720.10469578333296364No Hit
CTACAAGTTCCCTGGATGCTGGGTCTTCCAAATTACTTCCCACCCAGGTG4690.10403034403211854No Hit
TCACCATACAGCACTCAGGCAAGCTATTCTGTGTTGGGGTGAGTTGATGA4660.10336490473127342No Hit
GTTTACTAGTGCCATTTGTTCAGTGGTTCGCAGGGCTTTCCCCCACTGTT4650.10314309163099174No Hit
ATGTGGTATTGGGGGCCAAGTCTGTACAACATCTTGAGTCCCTTTTTACC4600.10203402612958322No Hit
TTGGAAGACCAACCTCCCATGCTGTAGCTCTTGTTCCCAAGAATATGGTG4600.10203402612958322No Hit
AGTTAAGGGAGTAGCCCCAGCGTTTGGTTTTATTAGGGTTCAAATGTATA4580.1015903999290198No Hit
TTATTAATAGATGTCAACAATATGTGGGCCCTCTTACAGTTAATGAAAAA4560.1011467737284564No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)