FastQCFastQC Report
Sat 3 Sep 2022
EGAF00002490262

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002490262
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences406063
Sequences flagged as poor quality0
Sequence length35-151
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN22310.5494221340038369No Hit
CCTCCCGCTTACAGACCACCAAATGCCCCTATCTTATCAACACTTCCGGA10320.25414775539756146No Hit
GTGTAAAAGGGGCAGCAAAGCCCAAAAGACCCACAATTCGTTGACATACT8990.2213942171534959No Hit
CATATAAAGGCATCAAGGCAGGATAGCCACATTGTGTAAAAGGGGCAGCA8780.2162226058518013No Hit
CCTATTAATAATGTCCTCCTGTAAATGAATGTTAGGAAAGGAGGGAGTTT8350.20563311604356957No Hit
GTATGTAAATAATGCCTAGTTTTAAAGTAATGATTAACTGCATGTTCAGG7420.18273026599320796No Hit
GACCTTTAACCTAATCTCCTCCCCCAACTCCTCCCAGTCTTTAAACAAAC7100.17484971543824482No Hit
TCTGTGGAGTTACTCTCTTTTTTGCCTTCTGACTTCTTTCCTTCTATTCG7040.17337211220918922No Hit
ATGTAAGACCTTGGGCAAGACCTGGTGGGCGTTCACGGTGGTCTCCATGC7020.17287957779950403No Hit
CCCTTAACTTCATGGGATATGTAATCGGAAGTTGGGGTACTTTACCACAG6900.16992437134139285No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGGAGCACCCACG6830.16820050090749464No Hit
ATCCTAACCTTACCAAATATTTGCCCTTGGATAAAGGCATTAAACCTTAT6830.16820050090749464No Hit
AGTCTAGACTCTGTGGTATTGTGAGGATTCTTGTCAACAAGAAAAACCCC6630.1632751568106427No Hit
CTTATATAGAATGCCAGCCTTCCACAGAGTATGTAAATAATGCCTAGTTT6470.1593348815331611No Hit
GTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATTGCTGAGAGTCCAA6390.15736474389442034No Hit
ATTAATAGGTGTCAACAATATGTGGGCCCTCTTACAATTAATGAAAAAAG6350.1563796750750499No Hit
ATACCACATCATCCATATAACTGAAAGCCAAACAGTGGGGGAAAGCCCTA6190.15243939979756838No Hit
GATCATTAACCTAATCTCCTCCCCCAACTCCTCCCAGTCTTTAAACAAAC5980.1472677884958738No Hit
GTCTTACATAAGAGGACTCTTGGACTCTCAGCAATGTCAACGACCGACCT5920.14579018526681822No Hit
CTCCCATAGGAATCTTGCGAAAGCCCAGGATGATGGGATGGGAATACAAG5820.1433275132183922No Hit
ATTGACAACAGTGCCAGCAGCGCCTCCTCCTGCCTCCACCAATCGGCAGT5710.14061857396512364No Hit
CTCTAAGGCCTCCCGATACAGAGCAGAGGCGGTGTCGAGGAGATCTCGAA5600.13790963471185505No Hit
CTATATAAGAGAGAAACTACACGCAGTGCTTCATTTTGTGGGTCACCATA5560.13692456589248467No Hit
TCCCGATACAGAGCAGAGGCGGTGTCGAGGAGATCTCGAATAGAAGGAAA5500.13544696266342907No Hit
GTGCTGGTGAACAGACCAATTTATGCCTACAGCCTCCTAGTACAAAGACC5400.1329842906150031No Hit
GGGTAAAGGTGCAGATATTGTTGACACAGAAAGGCCTTGTAAATTGGCGA5380.1324917562053179No Hit
CTTTATATGCGTGTATACAAGCTAAGCAGGCTTTCACTTTCTCGCCAATT5380.1324917562053179No Hit
GTATACACGCATATAAAGGCATCAAGGCAGGATAGCCACATTGTGTAAAA5300.1305216185665771No Hit
CTCCAAAAGACACCAAATACTCAAGAACAGTTTCTCTTCCAAAAGTAAGA5290.13027535136173451No Hit
GTATCGGGAGGCCTTAGAGTCTCCGGAACATTGTTCACCTCACCATACAG5210.12830521372299372No Hit
CATTTGAACCCTAATAAAACCAAACGATGGGGCTACTCCCTTAACTTCAT5200.12805894651815114No Hit
GTGGTAAAGTACCCCAACTTCCGATTACATATCCCATGAAGTTAAGGGAG5160.12707387769878073No Hit
CCTTTACCCCGTTGCCCGGCAACGGTCAGGTCTTTGCCAAGTGTTTGCTG5100.12559627446972513No Hit
ATGTGGTATTGGGGGCCAAGTCTGTACAACATCTTGAGTCCCTTTTTACC5070.12485747285519734No Hit
GTCCGGCAGATGAGAAGGCACAGACGGGGAGACCGCGTAAAGAGAGGTGC4970.12239480080677137No Hit
AGTCTGTACAACATCTTGAGTCCCTTTTTACCTCTATTACCAATTTTCTT4920.12116346478255838No Hit
GTATAAAGAATTTGGAGCTTCTGTGGAGTTACTCTCTTTTTTGCCTTCTG4920.12116346478255838No Hit
CTTTTGGAGTGTGGATTCGCACTCCTCCCGCTTACAGACCACCAAATGCC4900.12067093037287316No Hit
CTTTTACACAATGTGGCTATCCTGCCTTGATGCCTTTATATGCGTGTATA4890.12042466316803056No Hit
CTAGTAAACTGAGCCAGGAGAAACGGACTGAGGCCCACTCCCATAGGAAT4820.1187007927341324No Hit
CGTGTATACAAGCTAAGCAGGCTTTCACTTTCTCGCCAATTTACAAGGCC4710.11599185348086381No Hit
ATATGCTCCTGTGGTAAAGTACCCCAACTTCCGATTACATATCCCATGAA4710.11599185348086381No Hit
TCCCATGGCTGCTCGGGTGTGCTGCCAACTGGATCCTGCGCGGGACGTCC4670.1150067846614934No Hit
GTCCAAGAGTCCTCTTATGTAAGACCTTGGGCAAGACCTGGTGGGCGTTC4640.11426798304696562No Hit
GTTGTCAATATGCCCTGAGCCTGAGGGCTCCACCCCAAAAGACCGCCGTG4610.11352918143243781No Hit
CTACTGTACCTGTCTTTAACCCTGAGTGGCAAACTCCCTCCTTTCCTAAC4570.11254411261306743No Hit
ACATTGACCCGTATAAAGAATTTGGAGCTTCTGTGGAGTTACTCTCTTTT4500.11082024217916925No Hit
CAATATGCTCCTGTGGTAAAGTACCCCAACTTCCGATTACATATCCCATG4460.10983517335979885No Hit
GCCATGGGAAGGAGGTGTATTTCCGAGAGAGGACAACAGAGTTGTCAGTC4460.10983517335979885No Hit
ATTTACATACTCTGTGGAAGGCTGGCATTCTATATAAGAGAGAAACTACA4440.10934263895011366No Hit
AGTTAAGGGAGTAGCCCCATCGTTTGGTTTTATTAGGGTTCAAATGTATA4420.10885010454042844No Hit
CCTCTATGTTTCCCTCTTGTTGCTGTACAAAACCTTCGGACGGAAACTGC4360.10737250131137287No Hit
GCCTTAGAGTCTCCGGAACATTGTTCACCTCACCATACAGCACTCAGGCA4350.10712623410653027No Hit
ATATAAGAGAGAAACTACACGCAGTGCTTCATTTTGTGGGTCACCATATT4280.10540236367263209No Hit
CTCTTTACGCGGTCTCCCCGTCTGTGCCTTCTCATCTGCCGGACCGTGTG4260.10490982926294688No Hit
GTTTTAAAGTAATGATTAACTGCATGTTCAGGATAATAAGGTTTAATGCC4250.10466356205810429No Hit
TTGTAAGAGGGCCCACATATTGTTGACACCTATTAATAATGTCCTCCTGT4200.10343222603389131No Hit
GGTTAGGATAAAACCTAGCAGGCATAATTAATTTTAATCTCCTTTTTTCA4180.10293969162420609No Hit
GCACGGGACCATGCAAGACCTGCACGATTCCTGCTCAAGGAACCTCTATG4160.1024471572145209No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)