FastQCFastQC Report
Sat 3 Sep 2022
EGAF00002490263

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002490263
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences406063
Sequences flagged as poor quality0
Sequence length35-151
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN22300.5491758667989942No Hit
CCTCCCGCTTACAGACCACCAAATGCCCCTATCTTATCAACACTTCCGGA10080.2482373424813391No Hit
GTGTAAAAGGGGCAGCAAAGCCCAAAAGACCCACAATTCGTTGACATACT8830.2174539418760143No Hit
GTATGTAAATAATGCCTAGTTTTAAAGTAATGATTAACTGCATGTTCAGG8660.21326739939369013No Hit
CATATAAAGGCATCAAGGCAGGATAGCCACATTGTGTAAAAGGGGCAGCA8210.2021853751757732No Hit
CCTATTAATAATGTCCTCCTGTAAATGAATGTTAGGAAAGGAGGGAGTTT7600.1871630756803747No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGGAGCACCCACG7490.18445413642710615No Hit
GTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATTGCTGAGAGTCCAA7410.18248399878836535No Hit
CCCTTAACTTCATGGGATATGTAATCGGAAGTTGGGGTACTTTACCACAG7280.17928252512541157No Hit
ATCCTAACCTTACCAAATATTTGCCCTTGGATAAAGGCATTAAACCTTAT6970.17164824177529103No Hit
TCTGTGGAGTTACTCTCTTTTTTGCCTTCTGACTTCTTTCCTTCTATTCG6810.16770796649780945No Hit
CTTATATAGAATGCCAGCCTTCCACAGAGTATGTAAATAATGCCTAGTTT6580.16204382078642968No Hit
AGTCTAGACTCTGTGGTATTGTGAGGATTCTTGTCAACAAGAAAAACCCC6530.16081248476221668No Hit
ATGTAAGACCTTGGGCAAGACCTGGTGGGCGTTCACGGTGGTCTCCATGC6340.15613340787020732No Hit
ATTAATAGGTGTCAACAATATGTGGGCCCTCTTACAATTAATGAAAAAAG6300.15514833905083694No Hit
GTCTTACATAAGAGGACTCTTGGACTCTCAGCAATGTCAACGACCGACCT6220.15317820141209615No Hit
CTATATAAGAGAGAAACTACACGCAGTGCTTCATTTTGTGGGTCACCATA6140.15120806377335538No Hit
TCCCATGGCTGCTCGGGTGTGCTGCCAACTGGATCCTGCGCGGGACGTCC6030.1484991245200868No Hit
GTATCGGGAGGCCTTAGAGTCTCCGGAACATTGTTCACCTCACCATACAG5910.1455439180619756No Hit
CTCCCATAGGAATCTTGCGAAAGCCCAGGATGATGGGATGGGAATACAAG5800.14283497880870702No Hit
CTTTATATGCGTGTATACAAGCTAAGCAGGCTTTCACTTTCTCGCCAATT5770.14209617719417922No Hit
GTATAAAGAATTTGGAGCTTCTGTGGAGTTACTCTCTTTTTTGCCTTCTG5720.14086484116996623No Hit
ATACCACATCATCCATATAACTGAAAGCCAAACAGTGGGGGAAAGCCCTA5710.14061857396512364No Hit
GGGTAAAGGTGCAGATATTGTTGACACAGAAAGGCCTTGTAAATTGGCGA5710.14061857396512364No Hit
CTCCAAAAGACACCAAATACTCAAGAACAGTTTCTCTTCCAAAAGTAAGA5650.13914097073606807No Hit
GACCTTTAACCTAATCTCCTCCCCCAACTCCTCCCAGTCTTTAAACAAAC5620.13840216912154024No Hit
GTATACACGCATATAAAGGCATCAAGGCAGGATAGCCACATTGTGTAAAA5600.13790963471185505No Hit
CTTTTACACAATGTGGCTATCCTGCCTTGATGCCTTTATATGCGTGTATA5480.13495442825374387No Hit
CCTTTACCCCGTTGCCCGGCAACGGTCAGGTCTTTGCCAAGTGTTTGCTG5330.1312604201811049No Hit
GTGCTGGTGAACAGACCAATTTATGCCTACAGCCTCCTAGTACAAAGACC5280.1300290841568919No Hit
CATTTGAACCCTAATAAAACCAAACGATGGGGCTACTCCCTTAACTTCAT5200.12805894651815114No Hit
ATGTGGTATTGGGGGCCAAGTCTGTACAACATCTTGAGTCCCTTTTTACC5180.12756641210846595No Hit
CTCTAAGGCCTCCCGATACAGAGCAGAGGCGGTGTCGAGGAGATCTCGAA5150.12682761049393812No Hit
CTTTTGGAGTGTGGATTCGCACTCCTCCCGCTTACAGACCACCAAATGCC5030.12387240403582696No Hit
ATTGACAACAGTGCCAGCAGCGCCTCCTCCTGCCTCCACCAATCGGCAGT5000.12313360242129916No Hit
GTTGTCAATATGCCCTGAGCCTGAGGGCTCCACCCCAAAAGACCGCCGTG4970.12239480080677137No Hit
CTCTTTACGCGGTCTCCCCGTCTGTGCCTTCTCATCTGCCGGACCGTGTG4890.12042466316803056No Hit
GTGGTAAAGTACCCCAACTTCCGATTACATATCCCATGAAGTTAAGGGAG4870.11993212875834537No Hit
AGTCTGTACAACATCTTGAGTCCCTTTTTACCTCTATTACCAATTTTCTT4770.1174694567099194No Hit
CCTCTATGTTTCCCTCTTGTTGCTGTACAAAACCTTCGGACGGAAACTGC4640.11426798304696562No Hit
ATATAAGAGAGAAACTACACGCAGTGCTTCATTTTGTGGGTCACCATATT4600.11328291422759522No Hit
TCCCGATACAGAGCAGAGGCGGTGTCGAGGAGATCTCGAATAGAAGGAAA4570.11254411261306743No Hit
ATATGCTCCTGTGGTAAAGTACCCCAACTTCCGATTACATATCCCATGAA4500.11082024217916925No Hit
ACATTGACCCGTATAAAGAATTTGGAGCTTCTGTGGAGTTACTCTCTTTT4500.11082024217916925No Hit
CTACTGTACCTGTCTTTAACCCTGAGTGGCAAACTCCCTCCTTTCCTAAC4490.11057397497432665No Hit
GTCCAAGAGTCCTCTTATGTAAGACCTTGGGCAAGACCTGGTGGGCGTTC4490.11057397497432665No Hit
GTTTTAAAGTAATGATTAACTGCATGTTCAGGATAATAAGGTTTAATGCC4420.10885010454042844No Hit
AGTTAAGGGAGTAGCCCCATCGTTTGGTTTTATTAGGGTTCAAATGTATA4400.10835757013074325No Hit
GATCATTAACCTAATCTCCTCCCCCAACTCCTCCCAGTCTTTAAACAAAC4340.10687996690168766No Hit
GCCATGGGAAGGAGGTGTATTTCCGAGAGAGGACAACAGAGTTGTCAGTC4240.10441729485326169No Hit
CTAGTAAACTGAGCCAGGAGAAACGGACTGAGGCCCACTCCCATAGGAAT4230.1041710276484191No Hit
GTTCAAATGTATACCCAAAGACAAAAGAAAATTGGTAATAGAGGTAAAAA4180.10293969162420609No Hit
CTTGTATTCCCATCCCATCATCCTGGGCTTTCGCAAGATTCCTATGGGAG4180.10293969162420609No Hit
ATTTACATACTCTGTGGAAGGCTGGCATTCTATATAAGAGAGAAACTACA4110.1012158211903079No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)