FastQCFastQC Report
Sat 3 Sep 2022
EGAF00002490264

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002490264
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences411423
Sequences flagged as poor quality0
Sequence length35-151
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TAGCCACACTGTGTAAAAGGGGCAGCAAAGCCCAAAAGACCCACAATTCT25300.6149388828529275No Hit
GTGTAAAAGGGGCAGCAAAGCCCAAAAGACCCACAATTCTTTGACATACT21570.5242779329303418No Hit
GTGTGGCTATCCTGCCTTGATGCCTTTATATGCATGTATACAAGCTAAGC18640.4530616907659514No Hit
CCCTCATAAAACCAAACGTTGGGGCTACTCCCTTAACTTCATGGGATATG17370.42219321719981623No Hit
TTATTAGGGTTCAAATGTATACCCAAAGACAAAAGAAAATTGGTAATAGA16660.4049360390644179No Hit
GTGCTGGTGAACAGACCAATTTATGCCTACAGCCTCCTAGTACAAAGATC16600.40347768598255324No Hit
ATGTGGTATTGGGGGCCAAGTCTGTACAACATCTTGAGTCCCTTTTTACC13730.33371979690002745No Hit
ATGTAGTATTGGGGGCCAAGTCTGTACAACATCTTGAGTCCCTTTTTACC13630.33128920843025306No Hit
CCTCCCGCTTACAGACCACCAAATGCCCCTATCTTATCAACACTTCCGGA13610.33080309073629816No Hit
GTATGTAAATAATGCCTAGTTTTGAAGTAATGATTAACTGCATGTTCAGG12510.3040666175687796No Hit
CCCTTAACTTCATGGGATATGTAATTGGAAGTTGGGGTACTTTACCACAG12370.30066379371109536No Hit
AGTCTGTACAACATCTTGAGTCCCTTTTTACCTCTATTACCAATTTTCTT12360.3004207348641179No Hit
ATACCACATCATCCATATAACTGAAAGCCAAACAGTGGGGGAAAGCCCTG11660.283406615575697No Hit
ATATGTTCCTGTGGTAAAGTACCCCAACTTCCAATTACATATCCCATGAA10750.2612882605007498No Hit
CTTTTACACAGTGTGGCTATCCTGCCTTGATGCCTTTATATGCATGTATA10710.2603160251128401No Hit
GTATACATGCATATAAAGGCATCAAGGCAGGATAGCCACACTGTGTAAAA9960.24208661158953193No Hit
GTACAACATCTTGAGTCCCTTTTTACCTCTATTACCAATTTTCTTTTGTC9860.23965602311975753No Hit
GTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATTGCTGAGAGTCCAA9710.2360101404150959No Hit
CTTATATAGAATGCCAGCCTTCCACAGAGTATGTAAATAATGCCTAGTTT9670.23503790502718613No Hit
GTACAATATGTTCCTGTGGTAAAGTACCCCAACTTCCAATTACATATCCC8820.2143779030341036No Hit
GGGTAAAGGTGCAGATATTGTTTACACAGAAAGGCCTTGTAAGTTGGCGA8400.20416943146105104No Hit
CTCTTACAGTTAATGAAAAAAGGAGATTAAAATTAATTATGCCTGCTAGG8210.19955131336847962No Hit
ATGTAAGACCTTGGGCAAGACCTGGTGGGCGTTCACGGTGGTCTCCATGC8180.19882213682754732No Hit
GCACGGGACCATGCAAGACCTGCACGATTCCTGCTCAAGGAACCTCTATG7670.1864261356316978No Hit
GTTATATGGATGATGTGGTATTGGGGGCCAAGTCTGTACAACATCTTGAG7610.18496778254983315No Hit
CAGTTATATGGATGATGTGGTATTGGGGGCCAAGTCTGTACAACATCTTG7350.17864825252841965No Hit
GTCTATTTACAGGCAGTTTCCGAAAACATTGCTTGAGTTTTAGTACAATA7270.17670378175260013No Hit
CATGTATACAAGCTAAGCAGGCTTTCACTTTCTCGCCAACTTACAAGGCC7260.17646072290562267No Hit
GTGGTAAAGTACCCCAACTTCCAATTACATATCCCATGAAGTTAAGGGAG7190.1747593109767806No Hit
TCTGTGGAGTTACTCTCTTTTTTGCCTTCTGACTTCTTTCCTTCTATTCG6990.16989813403723175No Hit
ATTTACAGGCAGTTTCCGAAAACATTGCTTGAGTTTTAGTACAATATGTT6970.16941201634327688No Hit
GCCATGGGAAGGAGGTGTATTTCCGAGAGAGGACAACAGAGTTGTCAGTC6860.166738369026525No Hit
TTATTAATAGATGTCAACAATATGTGGGCCCTCTTACAGTTAATGAAAAA6760.16430778055675058No Hit
GGAACATATTGTACTAAAACTCAAGCAATGTTTTCGGAAACTGCCTGTAA6760.16430778055675058No Hit
GTCCAAGAGTCCTCTTATGTAAGACCTTGGGCAAGACCTGGTGGGCGTTC6740.1638216628627957No Hit
GGTCTATTTACAGGCAGTTTCCGAAAACATTGCTTGAGTTTTAGTACAAT6670.16212025093395363No Hit
CTTTTTACCTCTATTACCAATTTTCTTTTGTCTTTGGGTATACATTTGAA6670.16212025093395363No Hit
ACATTGACCCGTATAAAGAATTTGGAGCTTCTGTGGAGTTACTCTCTTTT6470.15725907399440478No Hit
CAATAGGTCTATTTACAGGCAGTTTCCGAAAACATTGCTTGAGTTTTAGT6410.15580072091254013No Hit
TTGACATACTTTCCAATCAATAGGTCTATTTACAGGCAGTTTCCGAAAAC6360.15458542667765293No Hit
GTTTTAGTACAATATGTTCCTGTGGTAAAGTACCCCAACTTCCAATTACA6350.15434236783067548No Hit
GTGCAGAGGTGAAGCGAAGTGCACACGGACCGGCAGATGAGAAGGCACAG6300.15312707359578825No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN6270.15239789705485596No Hit
TCCCATGGCTGCTCGGGTGTGCTGCCAACTGGATCCTGCGCGGGACGTCC6220.15118260281996873No Hit
CCCTAATAAAACCAAACGTTGGGGCTACTCCCTTAACTTCATGGGATATG6100.14826589665623943No Hit
ATCCTAACCTTACCAAATATTTGCCCTTGGATAAAGGCATTAAACCTTAT5970.1451061316455327No Hit
CCTTTACCCCGTTGCCCGGCAACGGTCCGGTCTCTGCCAAGTGTTTGCTG5890.14316166086971316No Hit
CTGTACAACATCTTGAGTCCCTTTTTACCTCTATTACCAATTTTCTTTTG5800.14097413124691618No Hit
GTATAAAGAATTTGGAGCTTCTGTGGAGTTACTCTCTTTTTTGCCTTCTG5800.14097413124691618No Hit
GCCCAAAAGACCCACAATTCTTTGACATACTTTCCAATCAATAGGTCTAT5790.14073107239993876No Hit
GCCTTAGAGTCTCCGGAACATTGTTCACCTCACCATACAGCACTCAGGCA5780.1404880135529613No Hit
CTAGTAAACTGAGCCAGGAGAAACGGACTGAGGCCCACTCCCATAGGAAT5770.14024495470598386No Hit
TGCAAGACCTGCACGATTCCTGCTCAAGGAACCTCTATGTTTCCCTCTTG5740.13951577816505153No Hit
GCTTTGGGGCATGGACATTGACCCGTATAAAGAATTTGGAGCTTCTGTGG5630.1368421308482997No Hit
GCGAACCACTGAACAAATGGCACTAGTAAACTGAGCCAGGAGAAACGGAC5620.13659907200132224No Hit
CATTTGAACCCTCATAAAACCAAACGTTGGGGCTACTCCCTTAACTTCAT5610.1363560131543448No Hit
ATTTATGCCTACAGCCTCCTAGTACAAAGATCATTAACCTAATCTCCTCC5580.13562683661341246No Hit
CTCTTATATAGAATGCCAGCCTTCCACAGAGTATGTAAATAATGCCTAGT5540.13465460122550268No Hit
GTATTGGGGGCCAAGTCTGTACAACATCTTGAGTCCCTTTTTACCTCTAT5540.13465460122550268No Hit
GTTCAAATGTATACCCAAAGACAAAAGAAAATTGGTAATAGAGGTAAAAA5480.13319624814363804No Hit
CCTCTATGTTTCCCTCTTGTTGCTGTACAAAACCTTCGGACGGAAACTGC5380.13076565967386364No Hit
CTGGTGAACAGACCAATTTATGCCTACAGCCTCCTAGTACAAAGATCATT5280.1283350712040892No Hit
GTATGGATCGGCAGAGGAGCCACAAAGGTTCCACGCATGCGCCGATGGCC5240.12736283581617944No Hit
CATATAAAGGCATCAAGGCAGGATAGCCACACTGTGTAAAAGGGGCAGCA5230.12711977696920201No Hit
GTCGTTGACATTGCTGAGAGTCCAAGAGTCCTCTTATGTAAGACCTTGGG5180.1259044827343148No Hit
CTCTAAGGCCTCCCGATAAAGAGCAGAGGCGGTGTCGAGGAGATCTCGAA5150.1251753061933825No Hit
GCCCCATCCAGTGGGGGTTGCGTCAGCAAACACTTGGCAGAGACCGGACC5130.1246891884994276No Hit
ATATTGTACTAAAACTCAAGCAATGTTTTCGGAAACTGCCTGTAAATAGA5120.12444612965245014No Hit
GGATAATAAGGTTTAATGCCTTTATCCAAGGGCAAATATTTGGTAAGGTT5020.12201554118267573No Hit
GGTTAGGATAAAACCTAGCAGGCATAATTAATTTTAATCTCCTTTTTTCA5000.12152942348872084No Hit
GCTTGTATACATGCATATAAAGGCATCAAGGCAGGATAGCCACACTGTGT4980.12104330579476597No Hit
GTACTAAAACTCAAGCAATGTTTTCGGAAACTGCCTGTAAATAGACCTAT4950.12031412925383364No Hit
CCTCAAGGTCGGTCGTTGACATTGCTGAGAGTCCAAGAGTCCTCTTATGT4920.11958495271290133No Hit
ACATAGAGGTTCCTTGAGCAGGAATCGTGCAGGTCTTGCATGGTCCCGTG4890.11885577617196899No Hit
GTTCAAGCCTCCAAGCTGTGCCTTGGGTGGCTTTGGGGCATGGACATTGA4870.11836965847801412No Hit
GTCTTTAAACAAACAGTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACA4870.11836965847801412No Hit
TTCCCCCACTGTTTGGCTTTCAGTTATATGGATGATGTGGTATTGGGGGC4830.11739742309010434No Hit
AGTCTAGACTCTGCGGTATTGTGAGGATTCTTGTCAACAAGAAAAACCCC4780.11618212885521714No Hit
GTACAAAGATCATTAACCTAATCTCCTCCCCCAACTCCTCCCAGTCTTTA4720.1147237757733525No Hit
TTATGAGGGTTCAAATGTATACCCAAAGACAAAAGAAAATTGGTAATAGA4700.11423765807939762No Hit
CCTCCAAGCTGTGCCTTGGGTGGCTTTGGGGCATGGACATTGACCCGTAT4670.11350848153846528No Hit
GTGTAAACAATATCTGCACCTTTACCCCGTTGCCCGGCAACGGTCCGGTC4620.11229318730357808No Hit
ATACTACATCATCCATATAACTGAAAGCCAAACAGTGGGGGAAAGCCCTG4620.11229318730357808No Hit
GTAGAGGCCCAAACGGCCCCGAGACGGGTCGTCCGCGGGATTCAGCGCCG4610.11205012845660063No Hit
CTCCAAAAGACACCAAATACTCAAGAACAGTTTCTCTTCCAAAAGTAAGA4590.11156401076264574No Hit
TCCTAGTACAAAGATCATTAACCTAATCTCCTCCCCCAACTCCTCCCAGT4560.11083483422171343No Hit
CTCCCATAGGAATCTTGCGAAAGCCCAGGATGATGGGATGGGAATACAAG4560.11083483422171343No Hit
CTTTACCACAGGAACATATTGTACTAAAACTCAAGCAATGTTTTCGGAAA4560.11083483422171343No Hit
ATGTAATTGGAAGTTGGGGTACTTTACCACAGGAACATATTGTACTAAAA4550.11059177537473598No Hit
GTACAGACTTGGCCCCCAATACCACATCATCCATATAACTGAAAGCCAAA4550.11059177537473598No Hit
CTTGTATTCCCATCCCATCATCCTGGGCTTTCGCAAGATTCCTATGGGAG4410.1071889515170518No Hit
GTGATTCGCAGGGCTTTCCCCCACTGTTTGGCTTTCAGTTATATGGATGA4410.1071889515170518No Hit
CTATATAAGAGAGAAACTACACGCAGCGCTTCATTTTGTGGGTCACCATA4390.10670283382309691No Hit
GTCTTACATAAGAGGACTCTTGGACTCTCAGCAATGTCAACGACCGACCT4380.10645977497611948No Hit
GGATAGCCACACTGTGTAAAAGGGGCAGCAAAGCCCAAAAGACCCACAAT4350.10573059843518715No Hit
GCCTATGGCCAAGCCCCATCCAGTGGGGGTTGCGTCAGCAAACACTTGGC4320.10500142189425482No Hit
GACCAATTTATGCCTACAGCCTCCTAGTACAAAGATCATTAACCTAATCT4260.10354306881239017No Hit
GCCTAATCATCTCATGTTCATGTCCTACTGTTCAAGCCTCCAAGCTGTGC4200.10208471573052552No Hit
CTGTAAGAGGGCCCACATATTGTTGACATCTATTAATAATGTCCTCTTGT4170.1013555391895932No Hit
CATTTGAACCCTAATAAAACCAAACGTTGGGGCTACTCCCTTAACTTCAT4170.1013555391895932No Hit
CTTTAATCCTGAGTGGCAAACTCCCTCCTTTCCTAACATTCATTTACAAG4140.10062636264866087No Hit
CTCTTTACGCGGTCTCCCCGTCTGTGCCTTCTCATCTGCCGGTCCGTGTG4140.10062636264866087No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)