FastQCFastQC Report
Sat 3 Sep 2022
EGAF00002490267

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002490267
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences426464
Sequences flagged as poor quality0
Sequence length35-151
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATAATATGATCCTGTGGTAAAGTACCCCAACTTCCAATTACATATCCC20700.48538680873414874No Hit
ATATGATCCTGTGGTAAAGTACCCCAACTTCCAATTACATATCCCATGAA15050.3529020034516395No Hit
CTTTTTACCTCTATTACCAATTTTCTTTTGTCTTTGGGTATACATTTGAA12320.28888722143017936No Hit
GGTCTATTTACAGGCAGTTTTCGAAAACATTGCTTGAGTTTTTGTATAAT11970.28068019809409467No Hit
GTATACATGCATATAAAGGCATCAGAGCAGGATAGCCACATTGTGTAAAA11470.2689558790425452No Hit
ATTCTTTGACATACTTTCCAATCAATAGGTCTATTTACAGGCAGTTTTCG10550.24738313198769415No Hit
CCTTTATACCGCTATTACCAATTTTCTTTTGTCTTTGGGTATACATTTGA9890.23190703083964884No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN9870.23143805807758683No Hit
CCCTTAACTTCATGGGATATGTAATTGGAAGTTGGGGTACTTTACCACAG9320.21854130712088243No Hit
ATGTCAACAATATGTGGGCCCTCTTACAGTTAATGAAAAAAGGAGATTAA9270.21736887521572748No Hit
GTGTAAAAGGGGCAGCAAAGCCCAAAAGACCCACAATTCTTTGACATACT9160.21478952502438659No Hit
GTGCTGGTGAACAGACCAATTTATGCCTACAGCCTCCTAGTACAAAGATC8720.20447212425902303No Hit
GGATAGCCACATTGTGTAAAAGGGGCAGCAAAGCCCAAAAGACCCACAAT8240.1932167779695355No Hit
GTGGTAAAGTACCCCAACTTCCAATTACATATCCCATGAAGTTAAGGGAG7940.18618218653860585No Hit
CTTTATACCTCTATTACCAATTTTCTTTTGTCTTTGGGTATACATTTGAA7920.18571321377654387No Hit
GATGTGAACAATATGTGGGCCCTCTTACAGTTAATGAAAAAAGGAGATTA7870.18454078187138892No Hit
CTTATATAGAATGCCAGCCTTCCACAGAGTATGTAAATAATGCCTAGTTT7680.1800855406318001No Hit
ATATTATACAAAAACTCAAGCAATGTTTTCGAAAACTGCCTGTAAATAGA7280.1707060853905605No Hit
GTCTATTTACAGGCAGTTTTCGAAAACATTGCTTGAGTTTTTGTATAATA7210.1690646807233436No Hit
GTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATTGCTGAGAGTCCAA7110.1667198169130337No Hit
CTTTAGGGCATGGACATTGACCCATATAAAGAATTTGGAGCTTCTGTGGA7080.16601635776994073No Hit
GGTTAGGATAGAACCTAGCAGGCATAATTAATTTTAATCTCCTTTTTTCA6930.16249906205447587No Hit
CTCTTACAGTTAATGAAAAAAGGAGATTAAAATTAATTATGCCTGCTAGG6820.159919711863135No Hit
GGATCATATTATACAAAAACTCAAGCAATGTTTTCGAAAACTGCCTGTAA6790.15921625272004203No Hit
GGGTAAAGGTGCAGATATTGTTTACACAGAAAGGCCTTGTAAGTTGGCGA6120.1435056651909657No Hit
TCCCATGGCTGCTCGGATGTGCTGCCAACTGGATCCTGCGCGGGACGTCC5860.13740901928415997No Hit
CCCCTGGATGCTGGGTCTTCCAAATTACTTCCCACCCAGGTGGCCAGATT5810.13623658737900501No Hit
ATGTAAGACCTTGGGCAAGACCTGGTGGGCGTTCACGGTGGTCTCCATGC5800.13600210099797405No Hit
CCTTTATACCTCTATTACCAATTTTCTTTTGTCTTTGGGTATACATTTGA5630.1320158325204472No Hit
TTGTTCACATCTATTAATAATGTCCTCCTGTAAATGAATATTAGGAAAGG5610.13154685975838523No Hit
CTTTATACCGCTATTACCAATTTTCTTTTGTCTTTGGGTATACATTTGAA5540.1299054550911683No Hit
CTATATAAGAGAGAAACTACACGCAGCGCCTCATTTTGTGGGTCACCATA5430.1273261048998274No Hit
CTCTTATATAGAATGCCAGCCTTCCACAGAGTATGTAAATAATGCCTAGT5320.12474675470848653No Hit
GCTTTGGGGCATGGACATTGACCCATATAAAGAATTTGGAGCTTCTGTGG5270.12357432280333158No Hit
CTACTGTACCTGTCTTTAATCCTGAGTGGCAAACTCCCTCCTTTCCTAAT5150.12076048623095971No Hit
AAATCGACCTATTGATTGGAAAGTATGTCAAAGAATTGTGGGTCTTTTGG5140.12052599984992872No Hit
GCCATGGGAAGGAGGTGTATTTGCGAGAAAGGACAACAGAGTTGTCAGTC4960.1163052449913709No Hit
GACTTTGCCCCCAATACCACATCATCCATATAACTGAAAGCCAAACAGTG4910.11513281308621595No Hit
CCTTTACCCCGTTGCCCGGCAACGGTCTGGTCTCTGCCAAGTGTTTGCTG4900.11489832670518497No Hit
GCTTAGATTGTATACATGCATATAAAGGCATCAGAGCAGGATAGCCACAT4860.113960381181061No Hit
CTCCCATAGGAATCTTGCGAAAGCCCAGGATGATGGGATGGGAATACAAG4780.11208449013281307No Hit
GTTGACATCTATTAATAATGTCCTCCTGTAAATGAATATTAGGAAAGGAG4720.11067757184662715No Hit
CATATAAAGGCATCAGAGCAGGATAGCCACATTGTGTAAAAGGGGCAGCA4720.11067757184662715No Hit
AGTTAAGGGAGTAGCCCCAACGTTTGGTTTTATTAGGATTCAAATGTATA4700.11020859908456516No Hit
AGTCTAGACTCTGTGGTATTGTGAGGATTCTTGTCAACAAGAAAAACCCC4650.10903616717941021No Hit
GTCCCGTACTGGTGGTTGATGTTCCTGGAAGTAGAGGACAAACGGGCAAC4610.10809822165528625No Hit
GTCTTACATAAGAGGACTCTTGGACTCTCAGCAATGTCAACGACCGACCT4570.1071602761311623No Hit
GTCTTTGGGTATACATTTGAATCCTAATAAAACCAAACGTTGGGGCTACT4540.10645681698806934No Hit
CCACCAAATGCCCCTATCTTATCAACACTTCCGGAAACTACTGTTGTTAG4510.10575335784497636No Hit
GTACAATATGATCCTGTGGTAAAGTACCCCAACTTCCAATTACATATCCC4470.1048154123208524No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGGAGCACCCACG4460.10458092593982142No Hit
CATGTATACAATCTAAGCAGGCTTTCACTTTCTCGCCAACTTACAAGGCC4460.10458092593982142No Hit
CTTCATCTGCCGTTCCGGCCGACCACGGGGCGCACCTCTCTTTACGCGGT4390.10293952127260449No Hit
CATTTGAATCCTAATAAAACCAAACGTTGGGGCTACTCCCTTAACTTCAT4390.10293952127260449No Hit
GTATGTAAATAATGCCTAGTTTTAAAGTAATGATTAACTGTATGTTCAGG4330.10153260298641856No Hit
CTAGTAAACTGAGCCAGGAGAAACGGACTGAGGCCCACTCCCATAGGAAT4320.10129811660538755No Hit
GCTTTAGGGCATGGACATTGACCCATATAAAGAATTTGGAGCTTCTGTGG4300.10082914384332557No Hit
CTTTTACACAATGTGGCTATCCTGCTCTGATGCCTTTATATGCATGTATA4270.10012568470023261No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)