FastQCFastQC Report
Sat 3 Sep 2022
EGAF00002490270

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002490270
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences401888
Sequences flagged as poor quality0
Sequence length35-151
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATGTAAATAATGCCTAGTTTTGAAGTAATGATTAACTGCATGCTCAGG29090.7238335058523768No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN21640.5384584759933116No Hit
CTTATATAGAATGCCAGCCTTCCACAGAGTATGTAAATAATGCCTAGTTT20480.509594712954853No Hit
GTATAGGGAGGCCTTAGAGTCTCCGGAACATTGTTCACCTCACCATACAG17390.4327076200334422No Hit
TCCCTATACAGAGCAGAGGCGGTGTCGAGAAGATCTCGAATAGAAGGAAA17380.4324587944900072No Hit
GAAAAAAGGAGATTAAAATTAATTATGCCTGCTAGGTTTTATCCTAACCT17340.43146349231626724No Hit
ATCCTAACCTTACCAAATATTTACCCTTGGATAAAGGCATTAAACCTTAT17100.4254916792738275No Hit
GGATAATAAGGTTTAATGCCTTTATCCAAGGGTAAATATTTGGTAAGGTT16040.39911617166971897No Hit
CTCTTATATAGAATGCCAGCCTTCCACAGAGTATGTAAATAATGCCTAGT10530.26201329723704114No Hit
TTATTATCCTGAGCATGCAGTTAATCATTACTTCAAAACTAGGCATTATT10040.24982084560872683No Hit
GTGCTGGTGAACAGACCAATTTATGCCTACAGCCTCCTAGTACAAAGATC9800.24384903256628715No Hit
AGTCTAGACTCTGTGGTATTGTGAGGATTCTTGTCAACAAGAAAAACCCC9630.23961899832789235No Hit
GAGTATGTAAATAATGCCTAGTTTTGAAGTAATGATTAACTGCATGCTCA8850.2202106059399634No Hit
GAAAAGAGGAGATTAAAATTAATTATGCCTGCTAGGTTTTATCCTAACCT8830.21971295485309342No Hit
CTCTTACAGTTAATGAAAAGAGGAGATTAAAATTAATTATGCCTGCTAGG8470.21075523528943388No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGGAGCACCCACG8370.20826697985508402No Hit
CTGTAAGAGGGCCCACATATTGTTGACATCTATTGATAATGTCCTCCTGT8240.2050322477904292No Hit
CTCCAAAAGACACCAAATACTCAAGAACAGTTTCTCTTCCAAAAGTAAGA8160.2030416434429493No Hit
TCCACAGAGTATGTAAATAATGCCTAGTTTTGAAGTAATGATTAACTGCA7940.19756748148737957No Hit
ATATTTACCCTTGGATAAAGGCATTAAACCTTATTATCCTGAGCATGCAG7690.1913468429015049No Hit
GTGTAAAAGGGGCAGCAAAGCCCAAAAGACCCACAATTCGTTGACATACT7520.18711680866311012No Hit
CTCTTACAGTTAATGAAAAAAGGAGATTAAAATTAATTATGCCTGCTAGG7300.1816426467075404No Hit
GTTTTATCCTAACCTTACCAAATATTTACCCTTGGATAAAGGCATTAAAC7290.18139382116410543No Hit
CATTTAAACCCTAATAAAACCAAACGTTGGGGCTACTCCCTTAACTTCAT7290.18139382116410543No Hit
AGTCTGTACAACATCTTGAGTCCCTTTTTACCTCTATTACCAATTTTCTT7280.18114499562067043No Hit
ATTGACAACAGTGCCAGCAGCACCTCCTCCTGCCTCCACCAATCGGCAGT7270.18089617007723544No Hit
CTTTAATCCTGAGTGGCAAACTCCCTCCTTTCCTAACATTCATTTACAGG7180.17865674018632058No Hit
TCTGTGGAGTTACTCTCGTTTTTGCCTTCTGACTTTTTTCCTTCTATTCG7170.17840791464288558No Hit
CCTCTTTTCATTAACTGTAAGAGGGCCCACATATTGTTGACATCTATTGA7110.17691496138227567No Hit
ATATGTTCCTGTGGTAAAGTACCCCAACTTCCAATTACATATCCCATGAA6950.17293375268731587No Hit
TTCTTCTACTGTACCTGTCTTTAATCCTGAGTGGCAAACTCCCTCCTTTC6800.16920136953579107No Hit
ATGTTAATATGGGCCTAAAAATCAGACAACTATTGTGGTTTCACATTTCC6670.16596663747113624No Hit
CTATATAAGAGAGAAACTACACGAAGCGCTTCATTTTGTGGGTCACCATA6670.16596663747113624No Hit
CCCTTAACTTCATGGGATATGTAATTGGAAGTTGGGGTACTTTACCACAG6380.15875069671152162No Hit
GATTAACTGCATGCTCAGGATAATAAGGTTTAATGCCTTTATCCAAGGGT6260.15576479019030176No Hit
CTACTGTACCTGTCTTTAATCCTGAGTGGCAAACTCCCTCCTTTCCTAAC6210.15452066247312685No Hit
GTTTTGAAGTAATGATTAACTGCATGCTCAGGATAATAAGGTTTAATGCC6150.15302770921251693No Hit
ATGTAAGACCTTGGGCAAGACCTGGTGGGCGTTCACGGTGGTCTCCATGC5940.1478023728003822No Hit
GTATACATGCATATAAAGGCATCAAGGCAGGATAGCCACATTGTGTAAAA5900.14680707062664225No Hit
GTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATTGCTGAGAGTCCAA5640.1403376064973326No Hit
ACCCAGCATCCAGGGAATTAGTAGTCAGCTATGTCAATGTTAATATGGGC5560.1383470021498527No Hit
CCTACCGCTTACAGACCACCAAATGCCCCTATCTTATCAACACTTCCGGA5400.13436579345489288No Hit
GGTAAATATTTGGTAAGGTTAGGATAAAACCTAGCAGGCATAATTAATTT5380.13386814236802294No Hit
GTGTAGTTTCTCTCTTATATAGAATGCCAGCCTTCCACAGAGTATGTAAA5290.13162871247710806No Hit
CATGTATACAAGCTAAGCAGGCTTTCACCTTCTCGCCAACTTACAAGGCC5260.13088223584680309No Hit
ATGTGGTATTGGGGGCCAAGTCTGTACAACATCTTGAGTCCCTTTTTACC5240.13038458475993311No Hit
GGAACATATTGTACAAAAAATCAAGCAATGTTTTCGGAAACTGCCTGTAA5170.12864280595588823No Hit
CTCATAAGGTGGGAAACTTTACTGGGCTTTATTCTTCTACTGTACCTGTC5140.12789632932558326No Hit
GGGTAAAGGTGCAGATATTGTTTACACAGAAAGGCCTTGTAAGTTGGCGA5110.1271498526952783No Hit
ATTTACATACTCTGTGGAAGGCTGGCATTCTATATAAGAGAGAAACTACA5080.12640337606497332No Hit
GTTGTCAATATGCCCTGAGCCTGAGGGCTCCACCCCAAAAGACCGCCGTG5020.12491042280436342No Hit
ACATTGACCCGTATAAAGAATTTGGAGCTTCTGTGGAGTTACTCTCGTTT4890.12167569073970858No Hit
CCCTATACAGAGCAGAGGCGGTGTCGAGAAGATCTCGAATAGAAGGAAAA4860.12092921410940362No Hit
GTTAGTATCCCTTGGACTCATAAGGTGGGAAACTTTACTGGGCTTTATTC4840.12043156302253363No Hit
ATATAAGAGAGAAACTACACGAAGCGCTTCATTTTGTGGGTCACCATATT4810.11968508639222868No Hit
GCCATGGGAAGGAGGTGTATTTCCGAGAAAGGACAACAGAGTTGTCAGTC4800.11943626084879369No Hit
GTATAAAGAATTTGGAGCTTCTGTGGAGTTACTCTCGTTTTTGCCTTCTG4780.11893860976192373No Hit
CCTTTATCCAAGGGTAAATATTTGGTAAGGTTAGGATAAAACCTAGCAGG4710.11719683095787882No Hit
ATGTAAATAATGCCTAGTTTTGAAGTAATGATTAACTGCATGCTCAGGAT4680.11645035432757385No Hit
AGTTAAGGGAGTAGCCCCAACGTTTGGTTTTATTAGGGTTTAAATGTATA4640.1154550521538339No Hit
CTTTTACACAATGTGGCTATCCTGCCTTGATGCCTTTATATGCATGTATA4590.11421092443665898No Hit
GTCTATTTACAGGCAGTTTCCGAAAACATTGCTTGATTTTTTGTACAATA4530.11271797117604905No Hit
ATGCTGGGTCATCCAAATTACTTCCCACCCAGGTGGCCAGATTCATCAAC4510.11222032008917908No Hit
GTGGTAAAGTACCCCAACTTCCAATTACATATCCCATGAAGTTAAGGGAG4440.11047854128513417No Hit
CTCAAGAACAGTTTCTCTTCCAAAAGTAAGACAGGAAATGTGAAACCACA4430.1102297157416992No Hit
TCCCATGGCTGCTCGGGTGTGCTGCCAACTGGATCCTGCGCGGGACGTCC4430.1102297157416992No Hit
CATATAAAGGCATCAAGGCAGGATAGCCACATTGTGTAAAAGGGGCAGCA4420.10998089019826418No Hit
CCCTTGGACTCATAAGGTGGGAAACTTTACTGGGCTTTATTCTTCTACTG4420.10998089019826418No Hit
ATACAGAGCAGAGGCGGTGTCGAGAAGATCTCGAATAGAAGGAAAAAAGT4390.10923441356795924No Hit
ATGATTAACTGCATGCTCAGGATAATAAGGTTTAATGCCTTTATCCAAGG4350.10823911139421927No Hit
CTTCCACAGAGTATGTAAATAATGCCTAGTTTTGAAGTAATGATTAACTG4340.1079902858507843No Hit
GGTTAGGATAAAACCTAGCAGGCATAATTAATTTTAATCTCCTCTTTTCA4280.10649733259017438No Hit
GTACAACATCTTGAGTCCCTTTTTACCTCTATTACCAATTTTCTTTTGTC4250.10575085595986941No Hit
GGATAAAGGCATTAAACCTTATTATCCTGAGCATGCAGTTAATCATTACT4250.10575085595986941No Hit
CAGTTATATGGATGATGTGGTATTGGGGGCCAAGTCTGTACAACATCTTG4230.10525320487299944No Hit
ATACCACATCATCCATATAACTGAAAGCCAAACAGTGGGGGAAAGCCCTG4200.1045067282426945No Hit
GTCTTACATAAGAGGACTCTTGGACTCTCAGCAATGTCAACGACCGACCT4190.10425790269925948No Hit
CTCTAAGGCCTCCCTATACAGAGCAGAGGCGGTGTCGAGAAGATCTCGAA4150.10326260052551954No Hit
GTTATATGGATGATGTGGTATTGGGGGCCAAGTCTGTACAACATCTTGAG4120.1025161238952146No Hit
AACCTACTCTCCTCCCCCAACTCCTCCCAGTCTTTAAACAAACAGTCTTT4110.1022672983517796No Hit
TATTAACATTGACATAGCTGACTACTAATTCCCTGGATGCTGGGTCATCC4080.10152082172147465No Hit
GGTTAGGATAAAACCTAGCAGGCATAATTAATTTTAATCTCCTTTTTTCA4040.1005255195477347No Hit
GGATTAAAGACAGGTACAGTAGAAGAATAAAGCCCAGTAAAGTTTCCCAC4030.1002766940042997No Hit
GAATATGGTGACCCACAAAATGAAGCGCTTCGTGTAGTTTCTCTCTTATA4030.1002766940042997No Hit
GGTCTATTTACAGGCAGTTTCCGAAAACATTGCTTGATTTTTTGTACAAT4020.10002786846086471No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)