FastQCFastQC Report
Sat 3 Sep 2022
EGAF00002490271

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002490271
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences401888
Sequences flagged as poor quality0
Sequence length35-151
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATGTAAATAATGCCTAGTTTTGAAGTAATGATTAACTGCATGCTCAGG33380.8305796639859861No Hit
CTTATATAGAATGCCAGCCTTCCACAGAGTATGTAAATAATGCCTAGTTT22100.5499044509913209No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN21510.5352237439286567No Hit
GTATAGGGAGGCCTTAGAGTCTCCGGAACATTGTTCACCTCACCATACAG17590.43768413090214187No Hit
GGATAATAAGGTTTAATGCCTTTATCCAAGGGTAAATATTTGGTAAGGTT16910.42076399394856284No Hit
GAAAAAAGGAGATTAAAATTAATTATGCCTGCTAGGTTTTATCCTAACCT15040.37423361732622024No Hit
ATCCTAACCTTACCAAATATTTACCCTTGGATAAAGGCATTAAACCTTAT14080.3503463651564615No Hit
CTCTTATATAGAATGCCAGCCTTCCACAGAGTATGTAAATAATGCCTAGT13550.33715861135440717No Hit
TCCCTATACAGAGCAGAGGCGGTGTCGAGAAGATCTCGAATAGAAGGAAA11290.28092403853810016No Hit
GTGCTGGTGAACAGACCAATTTATGCCTACAGCCTCCTAGTACAAAGATC10360.2577832629986464No Hit
GAGTATGTAAATAATGCCTAGTTTTGAAGTAATGATTAACTGCATGCTCA10130.25206027549964166No Hit
TCTGTGGAGTTACTCTCGTTTTTGCCTTCTGACTTTTTTCCTTCTATTCG9480.23588661517636753No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGGAGCACCCACG9420.23439366191575764No Hit
AGTCTAGACTCTGTGGTATTGTGAGGATTCTTGTCAACAAGAAAAACCCC9360.2329007086551477No Hit
CTGTAAGAGGGCCCACATATTGTTGACATCTATTGATAATGTCCTCCTGT9060.2254359423520981No Hit
TTATTATCCTGAGCATGCAGTTAATCATTACTTCAAAACTAGGCATTATT8970.2231965124611832No Hit
CTCCAAAAGACACCAAATACTCAAGAACAGTTTCTCTTCCAAAAGTAAGA8940.22245003583087827No Hit
CTCTTACAGTTAATGAAAAGAGGAGATTAAAATTAATTATGCCTGCTAGG8830.21971295485309342No Hit
GTGTAAAAGGGGCAGCAAAGCCCAAAAGACCCACAATTCGTTGACATACT8010.19930926029142448No Hit
CCTCTTTTCATTAACTGTAAGAGGGCCCACATATTGTTGACATCTATTGA7840.19507922605302971No Hit
TCCACAGAGTATGTAAATAATGCCTAGTTTTGAAGTAATGATTAACTGCA7830.19483040050959471No Hit
GAAAAGAGGAGATTAAAATTAATTATGCCTGCTAGGTTTTATCCTAACCT7760.1930886217055498No Hit
CTCTTACAGTTAATGAAAAAAGGAGATTAAAATTAATTATGCCTGCTAGG7500.18661915757624015No Hit
CTTTAATCCTGAGTGGCAAACTCCCTCCTTTCCTAACATTCATTTACAGG7390.1838820765984553No Hit
GTTTTATCCTAACCTTACCAAATATTTACCCTTGGATAAAGGCATTAAAC7350.18288677442471535No Hit
GTATAAAGAATTTGGAGCTTCTGTGGAGTTACTCTCGTTTTTGCCTTCTG7060.17567083366510072No Hit
CCCTTAACTTCATGGGATATGTAATTGGAAGTTGGGGTACTTTACCACAG7040.17517318257823075No Hit
ATATTTACCCTTGGATAAAGGCATTAAACCTTATTATCCTGAGCATGCAG6970.17343140377418584No Hit
ATATGTTCCTGTGGTAAAGTACCCCAACTTCCAATTACATATCCCATGAA6930.1724361016004459No Hit
CATTTAAACCCTAATAAAACCAAACGTTGGGGCTACTCCCTTAACTTCAT6920.1721872760570109No Hit
TTCTTCTACTGTACCTGTCTTTAATCCTGAGTGGCAAACTCCCTCCTTTC6790.16895254399235607No Hit
ATGTTAATATGGGCCTAAAAATCAGACAACTATTGTGGTTTCACATTTCC6720.16721076518831116No Hit
GTTTTGAAGTAATGATTAACTGCATGCTCAGGATAATAAGGTTTAATGCC6560.16322955649335139No Hit
ACATTGACCCGTATAAAGAATTTGGAGCTTCTGTGGAGTTACTCTCGTTT6510.16198542877617644No Hit
CTATATAAGAGAGAAACTACACGAAGCGCTTCATTTTGTGGGTCACCATA6460.16074130105900153No Hit
AGTCTGTACAACATCTTGAGTCCCTTTTTACCTCTATTACCAATTTTCTT6410.15949717334182656No Hit
GTATACATGCATATAAAGGCATCAAGGCAGGATAGCCACATTGTGTAAAA6120.15228123258221196No Hit
CTACTGTACCTGTCTTTAATCCTGAGTGGCAAACTCCCTCCTTTCCTAAC5980.14879767497412213No Hit
GTGTAGTTTCTCTCTTATATAGAATGCCAGCCTTCCACAGAGTATGTAAA5860.1458117684529023No Hit
GATTAACTGCATGCTCAGGATAATAAGGTTTAATGCCTTTATCCAAGGGT5780.1438211641054224No Hit
ATTGACAACAGTGCCAGCAGCACCTCCTCCTGCCTCCACCAATCGGCAGT5770.14357233856198742No Hit
ATGTGGTATTGGGGGCCAAGTCTGTACAACATCTTGAGTCCCTTTTTACC5710.14207938530137748No Hit
GGTAAATATTTGGTAAGGTTAGGATAAAACCTAGCAGGCATAATTAATTT5540.1378493510629827No Hit
CCCTTGGACTCATAAGGTGGGAAACTTTACTGGGCTTTATTCTTCTACTG5240.13038458475993311No Hit
TCCCATGGCTGCTCGGGTGTGCTGCCAACTGGATCCTGCGCGGGACGTCC5240.13038458475993311No Hit
GTCTATTTACAGGCAGTTTCCGAAAACATTGCTTGATTTTTTGTACAATA5170.12864280595588823No Hit
GGGTAAAGGTGCAGATATTGTTTACACAGAAAGGCCTTGTAAGTTGGCGA5110.1271498526952783No Hit
CTCATAAGGTGGGAAACTTTACTGGGCTTTATTCTTCTACTGTACCTGTC5100.12690102715184332No Hit
GTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATTGCTGAGAGTCCAA5070.12615455052153834No Hit
CATGTATACAAGCTAAGCAGGCTTTCACCTTCTCGCCAACTTACAAGGCC5070.12615455052153834No Hit
ATTTACATACTCTGTGGAAGGCTGGCATTCTATATAAGAGAGAAACTACA5050.12565689943466835No Hit
ATGTAAGACCTTGGGCAAGACCTGGTGGGCGTTCACGGTGGTCTCCATGC5030.1251592483477984No Hit
GCTTTGGGGCATGGACATTGACCCGTATAAAGAATTTGGAGCTTCTGTGG4980.12391512063062346No Hit
ATGTAAATAATGCCTAGTTTTGAAGTAATGATTAACTGCATGCTCAGGAT4910.12217334182657855No Hit
GGAACATATTGTACAAAAAATCAAGCAATGTTTTCGGAAACTGCCTGTAA4890.12167569073970858No Hit
GTTGTCAATATGCCCTGAGCCTGAGGGCTCCACCCCAAAAGACCGCCGTG4860.12092921410940362No Hit
CTTTTACACAATGTGGCTATCCTGCCTTGATGCCTTTATATGCATGTATA4830.12018273747909866No Hit
CCTTTACCCCGTTGCCCGGCAACGGTCAGGTCTCTGCCAAGTGTTTGCTG4810.11968508639222868No Hit
ACCCAGCATCCAGGGAATTAGTAGTCAGCTATGTCAATGTTAATATGGGC4790.11918743530535872No Hit
ATATAAGAGAGAAACTACACGAAGCGCTTCATTTTGTGGGTCACCATATT4740.11794330758818376No Hit
CTTCCACAGAGTATGTAAATAATGCCTAGTTTTGAAGTAATGATTAACTG4660.11595270324070386No Hit
GTTAGTATCCCTTGGACTCATAAGGTGGGAAACTTTACTGGGCTTTATTC4650.11570387769726889No Hit
CTCAAGAACAGTTTCTCTTCCAAAAGTAAGACAGGAAATGTGAAACCACA4620.11495740106696392No Hit
GAATATGGTGACCCACAAAATGAAGCGCTTCGTGTAGTTTCTCTCTTATA4500.11197149454574408No Hit
ATTTACAGGCAGTTTCCGAAAACATTGCTTGATTTTTTGTACAATATGTT4430.1102297157416992No Hit
ATGATTAACTGCATGCTCAGGATAATAAGGTTTAATGCCTTTATCCAAGG4330.10774146030734931No Hit
GTACAACATCTTGAGTCCCTTTTTACCTCTATTACCAATTTTCTTTTGTC4320.10749263476391432No Hit
CCTTTATCCAAGGGTAAATATTTGGTAAGGTTAGGATAAAACCTAGCAGG4310.10724380922047932No Hit
GTCTTACATAAGAGGACTCTTGGACTCTCAGCAATGTCAACGACCGACCT4260.1059996815033044No Hit
GGTTAGGATAAAACCTAGCAGGCATAATTAATTTTAATCTCCTCTTTTCA4240.10550203041643444No Hit
CTCTTTACGCGGTCTCCCCGTCTGTGCCTTCTCATCTGCCGGACCGTGTG4230.10525320487299944No Hit
CCTACCGCTTACAGACCACCAAATGCCCCTATCTTATCAACACTTCCGGA4160.10351142606895454No Hit
CAATAGGTCTATTTACAGGCAGTTTCCGAAAACATTGCTTGATTTTTTGT4140.10301377498208455No Hit
CAGTTATATGGATGATGTGGTATTGGGGGCCAAGTCTGTACAACATCTTG4140.10301377498208455No Hit
AGTTAAGGGAGTAGCCCCAACGTTTGGTTTTATTAGGGTTTAAATGTATA4140.10301377498208455No Hit
GCTCAGGATAATAAGGTTTAATGCCTTTATCCAAGGGTAAATATTTGGTA4110.1022672983517796No Hit
GGATAAAGGCATTAAACCTTATTATCCTGAGCATGCAGTTAATCATTACT4100.10201847280834461No Hit
GCCATGGGAAGGAGGTGTATTTCCGAGAAAGGACAACAGAGTTGTCAGTC4100.10201847280834461No Hit
GGATTAAAGACAGGTACAGTAGAAGAATAAAGCCCAGTAAAGTTTCCCAC4100.10201847280834461No Hit
GGTCTATTTACAGGCAGTTTCCGAAAACATTGCTTGATTTTTTGTACAAT4080.10152082172147465No Hit
CCAAAAGACACCAAATACTCAAGAACAGTTTCTCTTCCAAAAGTAAGACA4080.10152082172147465No Hit
CATATAAAGGCATCAAGGCAGGATAGCCACATTGTGTAAAAGGGGCAGCA4070.10127199617803964No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)