FastQCFastQC Report
Sat 3 Sep 2022
EGAF00002490272

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002490272
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences441731
Sequences flagged as poor quality0
Sequence length35-151
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATATAGAATGCCAGCCTTCCACAGAGTATGTAAATAATGTCTAGTTT12160.27528065723256917No Hit
GTGCTGGTGAACAGACCAATTTATGCCTACAGCCTCCTAGTACAAAGATC11390.2578492340360989No Hit
ATTAATAGGTGTCAACAATATGTGGGCCCTCTTACTGTTAATGAAAAAAG11240.2544535022445787No Hit
GTATGTAAATAATGTCTAGTTTTGAAGTAATGATTAACTGCATGTTCAGG10830.2451718353477569No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN10280.23272081877884956No Hit
CCTCCCGCTTACAGACCACCAAATGCCCCTATCTTATCAACACTTCCGGA10170.23023061546506812No Hit
CCTATTAATAATGTCCTCCTGTAAATGAATGTTAGGAAAAGAGGGAGTTT9240.20917707835764301No Hit
GTCCAAGAGTCCTCTTATGTAAGACCTTGGGCAAGACCTGGTGGGCGTTC9070.20532858232725346No Hit
CCCTTAACTTCATGGGATATGTAATTGGAAGTTGGGGTACTCTGCCACAG8980.20329114325234135No Hit
GTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATTGCGGAGAGTCCAA8860.2005745578191252No Hit
GTGTAAATGGGGCAGCAAAGCCCAAAAGACCCACAATTCTCTGACATACT8780.1987635008636478No Hit
GTATACATGCATATAAAGGCATCAAGGCAGGATAGCCACATTGTGTAAAT8640.1955941511915623No Hit
ATGTAAGACCTTGGGCAAGACCTGGTGGGCGTTCACGGTGGTCTCCATGC8160.1847278094586977No Hit
CTCTTATATAGAATGCCAGCCTTCCACAGAGTATGTAAATAATGTCTAGT7630.17272955712865976No Hit
TTGTTTACACAGAAAGGCCTTGTAAGTTGGCGAGAAAGTGAAAGCCTGCT7540.17069211805374765No Hit
ATCCTAACCTTACCAAGTACTTGCCCTTGGATAAAGGCATTAAACCTTAT7500.16978658957600895No Hit
AGTCTAGACTCTGTGGTATTGTGAGGATTCTTGTCAACAAGAAAAACCCC7210.16322150811240324No Hit
GCACTGGACCATGCAAGACCTGCACGATTCCTGCTCAAGGAACCTCTATG7060.15982577632088307No Hit
CTATATAAGAGAGAAACTACACGCAGCGCTTCATTTTGTGGGTCACCATA6950.1573355730071016No Hit
TTATTAATAGGTGTCAACAATATGTGGGCCCTCTTACTGTTAATGAAAAA6810.15416622333501612No Hit
TCTGTGGAGTTACTCTCTTTTTTGCCTTCTGACTTCTTTCCTTCTATTCG6800.15393984121558144No Hit
CTCTTACTGTTAATGAAAAAAGGAGATTAAAATTAATTATGCCTGCTAGG6750.15280793061840803No Hit
CATATAAAGGCATCAAGGCAGGATAGCCACATTGTGTAAATGGGGCAGCA6740.15258154849897335No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGGAGCACCCACG6600.14941219882688786No Hit
CATGTATACAAGCTAAGCAGGCTTTCACTTTCTCGCCAACTTACAAGGCC6560.14850667034914913No Hit
GGTTAGGATAAAACCTAGCAGGCATAATTAATTTTAATCTCCTTTTTTCA6390.1446581743187596No Hit
GGATAATAAGGTTTAATGCCTTTATCCAAGGGCAAGTACTTGGTAAGGTT6280.14216797100497813No Hit
TCCCATGGCTGCTCGGGTGTGCTGCCAACTGGATCCTGCGCGGGACGTCC6190.14013053193006603No Hit
CATTTACACAATGTGGCTATCCTGCCTTGATGCCTTTATATGCATGTATA6180.13990414981063135No Hit
GCCATGGGAAGGAGGTGTATTTCCGAGAGAGGACAACAGAGTTGTCAGTC6150.13922500345232733No Hit
CATTTGAACCCTAATAAAACCAAACGCTGGGGCTACTCCCTTAACTTCAT6130.13877223921345797No Hit
GGAAAATATAGGCCCCTCACTCTGGGATCTAGCAGAGCTTGGTGGAATGT6130.13877223921345797No Hit
CAGTTATATGGATGATGTGGTATTGGGGGCCAAGTCTGTACAACATCTTG6090.13786671073571924No Hit
AGTCTGTACAACATCTTGAGTCCCTTTTTACCTCTATTACCAATTTTCTT6030.13650841801911118No Hit
CAATATGTGGGCCCTCTTACTGTTAATGAAAAAAGGAGATTAAAATTAAT6000.13582927166080713No Hit
GTATCGGGAGGCCCTAGAGTCTCCGGAACATTGTTCACCTCACCATACAG5970.1351501253025031No Hit
GTATAAAGAATTTGGAGCTTCTGTGGAGTTACTCTCTTTTTTGCCTTCTG5940.13447097894419907No Hit
ATATGTTCCTGTGGCAGAGTACCCCAACTTCCAATTACATATCCCATGAA5830.1319807756304176No Hit
GTCCAGTGCTGGTAGTTGATGTTCCTGGAAGTAGAGGACAAACGGGCAAC5820.13175439351098292No Hit
CTCCCATAGGAATCTTGCGAAAGCCCAGGATGATGGGATGGGAATACAAG5800.13130162927211358No Hit
GGAACATATTGTACTAAAACTCAAGCAATGTTTTCGGAAACTGCCTGTAA5800.13130162927211358No Hit
ATATAAGAGAGAAACTACACGCAGCGCTTCATTTTGTGGGTCACCATATT5670.12835866171946275No Hit
ATATCTTCCTGCTGGTGGCTCCAGTTCCGGAACAGTACACCCTGTTCCGA5510.1247365478085079No Hit
CACATATTGTTGACACCTATTAATAATGTCCTCCTGTAAATGAATGTTAG5460.12360463721133451No Hit
TCCCGATACAGAGCAGAGGCGGTGTCGAGGAGATCTCGAATAGAAGGAAA5190.11749231998659819No Hit
CTAGTAAACTGAGCCAGGAGAAACGGACTGAGGCCCACTCCCATAGGAAT5190.11749231998659819No Hit
ATACCACATCATCCATATAACTGAAAGCCAAACAGTGGGGGAAAGCCCTA5160.11681317362829415No Hit
GTTATATGGATGATGTGGTATTGGGGGCCAAGTCTGTACAACATCTTGAG5110.11568126303112074No Hit
GTGTAATCAATATCTGAACCTTTACCCCGTTGCACGGCAACGGTCAGGTC5070.11477573455338204No Hit
GTCTATTTACAGGCAGTTTCCGAAAACATTGCTTGAGTTTTAGTACAATA5060.11454935243394736No Hit
GTCCGGCAGATGAGAAGGCACAGACGGGGAGACCGCGTAAAGAGAGGTGC5020.11364382395620863No Hit
CTCTAGGGCCTCCCGATACAGAGCAGAGGCGGTGTCGAGGAGATCTCGAA4970.11251191335903525No Hit
AGTTAAGGGAGTAGCCCCAGCGTTTGGTTTTATTAGGGTTCAAATGTATA4970.11251191335903525No Hit
ATATTTTCCTGCTGGTGGCTCCAGTTCCGGAACAGTACACCCTGTTCCGA4900.1109272385229925No Hit
GATTAACTGCATGTTCAGGATAATAAGGTTTAATGCCTTTATCCAAGGGC4800.10866341732864572No Hit
GTCTTACATAAGAGGACTCTTGGACTCTCCGCAATGTCAACGACCGACCT4790.10843703520921104No Hit
GCCTAATCATCTCATGTTCATGTCCTACTGTTCAAGCCTCCAAGCTGTGC4750.10753150673147233No Hit
ACTGACAACAGTGCCAGCAGCGCCTCCTCCTGCCTCCACCAATCGGCAGT4690.10617321401486426No Hit
GCCCTAGAGTCTCCGGAACATTGTTCACCTCACCATACAGCACTCAGGCA4680.10594683189542957No Hit
ATTTATGCCTACAGCCTCCTAGTACAAAGATCATTAACCTAATCTCCTCC4660.10549406765656022No Hit
GTTCAAGCCTCCAAGCTGTGCCTTGGGTGGCTTTGGGGCATGGACATTGA4600.10413577493995216No Hit
ATGTGGTATTGGGGGCCAAGTCTGTACAACATCTTGAGTCCCTTTTTACC4590.10390939282051746No Hit
CTCATAAGGTGGGAAACTTTACTGGGCTTTATTCTTCTACCGTACCTGTC4580.10368301070108278No Hit
CTGGTGAACAGACCAATTTATGCCTACAGCCTCCTAGTACAAAGATCATT4580.10368301070108278No Hit
GTATGGATCGGCAGAGGAGCCACAAAGGTTCCACGCATGCGCCGATGGCC4580.10368301070108278No Hit
ACATTGACCCGTATAAAGAATTTGGAGCTTCTGTGGAGTTACTCTCTTTT4570.10345662858164811No Hit
GTACAACATCTTGAGTCCCTTTTTACCTCTATTACCAATTTTCTTTTGTC4540.10277748222334408No Hit
ATCCAAGGGCAAGTACTTGGTAAGGTTAGGATAAAACCTAGCAGGCATAA4540.10277748222334408No Hit
ATCTCAATGTTAGTATCCCTTGGACTCATAAGGTGGGAAACTTTACTGGG4540.10277748222334408No Hit
GTACTAAAACTCAAGCAATGTTTTCGGAAACTGCCTGTAAATAGACCTAT4530.10255110010390939No Hit
ATTGATAGGTGTCAACAATATGTGGGCCCTCTTACTGTTAATGAAAAAAG4510.10209833586504004No Hit
CCTCTATGTTTCCCTCTTGTTGCTGTACAAAACCTTCGGACGGAAACTGC4490.10164557162617067No Hit
GTACAATATGTTCCTGTGGCAGAGTACCCCAACTTCCAATTACATATCCC4480.10141918950673601No Hit
GCACGATTCCTGCTCAAGGAACCTCTATGTTTCCCTCTTGTTGCTGTACA4450.10074004314843198No Hit
GTCGTTGACATTGCGGAGAGTCCAAGAGTCCTCTTATGTAAGACCTTGGG4420.10006089679012793No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)