FastQCFastQC Report
Sat 3 Sep 2022
EGAF00002490273

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002490273
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences441731
Sequences flagged as poor quality0
Sequence length35-151
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATGTAAATAATGTCTAGTTTTGAAGTAATGATTAACTGCATGTTCAGG13400.3033520400424693No Hit
CTTATATAGAATGCCAGCCTTCCACAGAGTATGTAAATAATGTCTAGTTT12200.27618618571030784No Hit
GTGCTGGTGAACAGACCAATTTATGCCTACAGCCTCCTAGTACAAAGATC11940.2703002506050062No Hit
ATTAATAGGTGTCAACAATATGTGGGCCCTCTTACTGTTAATGAAAAAAG11840.2680364294106594No Hit
CCTCCCGCTTACAGACCACCAAATGCCCCTATCTTATCAACACTTCCGGA10790.24426630687001816No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN10200.23090976182337217No Hit
GTGTAAATGGGGCAGCAAAGCCCAAAAGACCCACAATTCTCTGACATACT9310.21076175319368573No Hit
CCCTTAACTTCATGGGATATGTAATTGGAAGTTGGGGTACTCTGCCACAG8810.19944264722195185No Hit
CCTATTAATAATGTCCTCCTGTAAATGAATGTTAGGAAAAGAGGGAGTTT8650.19582053331099694No Hit
GTATACATGCATATAAAGGCATCAAGGCAGGATAGCCACATTGTGTAAAT8590.1944622405943889No Hit
GTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATTGCGGAGAGTCCAA8390.18993459820569533No Hit
CTCTTATATAGAATGCCAGCCTTCCACAGAGTATGTAAATAATGTCTAGT8280.18744439489191386No Hit
GTCCAAGAGTCCTCTTATGTAAGACCTTGGGCAAGACCTGGTGGGCGTTC7740.1752197604424412No Hit
TCCCATGGCTGCTCGGGTGTGCTGCCAACTGGATCCTGCGCGGGACGTCC7740.1752197604424412No Hit
TTGTTTACACAGAAAGGCCTTGTAAGTTGGCGAGAAAGTGAAAGCCTGCT7560.171144882292617No Hit
TTATTAATAGGTGTCAACAATATGTGGGCCCTCTTACTGTTAATGAAAAA7440.16842829685940086No Hit
CTCTTACTGTTAATGAAAAAAGGAGATTAAAATTAATTATGCCTGCTAGG7310.16548532930675003No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGGAGCACCCACG7300.16525894718731535No Hit
AGTCTAGACTCTGTGGTATTGTGAGGATTCTTGTCAACAAGAAAAACCCC7190.1627687438735339No Hit
CTATATAAGAGAGAAACTACACGCAGCGCTTCATTTTGTGGGTCACCATA6970.15778833724597097No Hit
GTATAAAGAATTTGGAGCTTCTGTGGAGTTACTCTCTTTTTTGCCTTCTG6720.152128784260104No Hit
CAATATGTGGGCCCTCTTACTGTTAATGAAAAAAGGAGATTAAAATTAAT6500.14714837763254107No Hit
ATGTAAGACCTTGGGCAAGACCTGGTGGGCGTTCACGGTGGTCTCCATGC6410.14511093855762897No Hit
GCACTGGACCATGCAAGACCTGCACGATTCCTGCTCAAGGAACCTCTATG6400.14488455643819428No Hit
GGTTAGGATAAAACCTAGCAGGCATAATTAATTTTAATCTCCTTTTTTCA6250.14148882464667412No Hit
GTGTAATCAATATCTGAACCTTTACCCCGTTGCACGGCAACGGTCAGGTC6230.14103606040780475No Hit
CATGTATACAAGCTAAGCAGGCTTTCACTTTCTCGCCAACTTACAAGGCC6200.14035691404950074No Hit
TCTGTGGAGTTACTCTCTTTTTTGCCTTCTGACTTCTTTCCTTCTATTCG6150.13922500345232733No Hit
CATATAAAGGCATCAAGGCAGGATAGCCACATTGTGTAAATGGGGCAGCA6080.13764032861628459No Hit
ATATGTTCCTGTGGCAGAGTACCCCAACTTCCAATTACATATCCCATGAA6070.1374139464968499No Hit
ATCCTAACCTTACCAAGTACTTGCCCTTGGATAAAGGCATTAAACCTTAT6060.13718756437741522No Hit
GCCATGGGAAGGAGGTGTATTTCCGAGAGAGGACAACAGAGTTGTCAGTC6010.13605565378024181No Hit
GTATCGGGAGGCCCTAGAGTCTCCGGAACATTGTTCACCTCACCATACAG6000.13582927166080713No Hit
CATTTGAACCCTAATAAAACCAAACGCTGGGGCTACTCCCTTAACTTCAT5990.13560288954137248No Hit
CATTTACACAATGTGGCTATCCTGCCTTGATGCCTTTATATGCATGTATA5890.1333390683470257No Hit
GGAACATATTGTACTAAAACTCAAGCAATGTTTTCGGAAACTGCCTGTAA5840.13220715774985228No Hit
GTCTATTTACAGGCAGTTTCCGAAAACATTGCTTGAGTTTTAGTACAATA5820.13175439351098292No Hit
ATATAAGAGAGAAACTACACGCAGCGCTTCATTTTGTGGGTCACCATATT5750.13016971867494018No Hit
GTCTTACATAAGAGGACTCTTGGACTCTCCGCAATGTCAACGACCGACCT5660.12813227960002807No Hit
GGATAATAAGGTTTAATGCCTTTATCCAAGGGCAAGTACTTGGTAAGGTT5650.1279058974805934No Hit
AGTCTGTACAACATCTTGAGTCCCTTTTTACCTCTATTACCAATTTTCTT5620.12722675112228934No Hit
ATGTGGTATTGGGGGCCAAGTCTGTACAACATCTTGAGTCCCTTTTTACC5580.12632122264455065No Hit
GGAAAATATAGGCCCCTCACTCTGGGATCTAGCAGAGCTTGGTGGAATGT5460.12360463721133451No Hit
CTCCCATAGGAATCTTGCGAAAGCCCAGGATGATGGGATGGGAATACAAG5420.12269910873359578No Hit
GTCCAGTGCTGGTAGTTGATGTTCCTGGAAGTAGAGGACAAACGGGCAAC5400.12224634449472643No Hit
CTCTTTACGCGGTCTCCCCGTCTGTGCCTTCTCATCTGCCGGACCGTGTG5350.12111443389755303No Hit
CAGTTATATGGATGATGTGGTATTGGGGGCCAAGTCTGTACAACATCTTG5300.11998252330037965No Hit
GCCCTAGAGTCTCCGGAACATTGTTCACCTCACCATACAGCACTCAGGCA5290.11975614118094496No Hit
GCCTAATCATCTCATGTTCATGTCCTACTGTTCAAGCCTCCAAGCTGTGC5280.1195297590615103No Hit
ATATCTTCCTGCTGGTGGCTCCAGTTCCGGAACAGTACACCCTGTTCCGA5020.11364382395620863No Hit
CTCTAGGGCCTCCCGATACAGAGCAGAGGCGGTGTCGAGGAGATCTCGAA4970.11251191335903525No Hit
GTTATATGGATGATGTGGTATTGGGGGCCAAGTCTGTACAACATCTTGAG4970.11251191335903525No Hit
GTCCTACTGTTCAAGCCTCCAAGCTGTGCCTTGGGTGGCTTTGGGGCATG4930.11160638488129652No Hit
ATACCACATCATCCATATAACTGAAAGCCAAACAGTGGGGGAAAGCCCTA4890.11070085640355783No Hit
ATATTTTCCTGCTGGTGGCTCCAGTTCCGGAACAGTACACCCTGTTCCGA4890.11070085640355783No Hit
ATATTGTACTAAAACTCAAGCAATGTTTTCGGAAACTGCCTGTAAATAGA4840.10956894580638442No Hit
GTTCAAATGTATACCCAAAGACAAAAGAAAATTGGTAATAGAGGTAAAAA4820.10911618156751507No Hit
GATTAACTGCATGTTCAGGATAATAAGGTTTAATGCCTTTATCCAAGGGC4800.10866341732864572No Hit
GTACTAAAACTCAAGCAATGTTTTCGGAAACTGCCTGTAAATAGACCTAT4770.10798427097034169No Hit
ACATTGACCCGTATAAAGAATTTGGAGCTTCTGTGGAGTTACTCTCTTTT4740.10730512461203764No Hit
GAGTATGTAAATAATGTCTAGTTTTGAAGTAATGATTAACTGCATGTTCA4720.1068523603731683No Hit
TCCCGATACAGAGCAGAGGCGGTGTCGAGGAGATCTCGAATAGAAGGAAA4700.10639959613429892No Hit
CCTTTACCCCGTTGCACGGCAACGGTCAGGTCTCTGCCAAGTGTTTGCTG4690.10617321401486426No Hit
CCTCTATGTTTCCCTCTTGTTGCTGTACAAAACCTTCGGACGGAAACTGC4680.10594683189542957No Hit
GTTCAAGCCTCCAAGCTGTGCCTTGGGTGGCTTTGGGGCATGGACATTGA4660.10549406765656022No Hit
ATTTATGCCTACAGCCTCCTAGTACAAAGATCATTAACCTAATCTCCTCC4650.10526768553712554No Hit
ATTGATAGGTGTCAACAATATGTGGGCCCTCTTACTGTTAATGAAAAAAG4610.10436215705938681No Hit
GGATGGGGCTTGGCCATAGGCCATCGGCGCATGCGTGGAACCTTTGTGGC4540.10277748222334408No Hit
GTCTACGTCCCGTCGGCGCTGAATCCCGCGGACGACCCGTCTCGGGGCCG4490.10164557162617067No Hit
GTACAATATGTTCCTGTGGCAGAGTACCCCAACTTCCAATTACATATCCC4480.10141918950673601No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)