FastQCFastQC Report
Sat 3 Sep 2022
EGAF00002490274

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002490274
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences383669
Sequences flagged as poor quality0
Sequence length35-151
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATGTAAATAATGCCTAGTTTTGAAGTAATGATTAACTGCATGTTCAGG28550.7441310087601553No Hit
CTTATATAGAATGCCAGCCTTCCACAGAGTATGTAAATAATGCCTAGTTT21440.5588150202387996No Hit
CTCTTACAGTTAATGAAAAAAGGAGATTAAAATTAATCATGCCTGCTAGG19340.5040803400848127No Hit
TTCTTAATAGATGTCAACAATATGTGGGCCCTCTTACAGTTAATGAAAAA14990.39070135976583986No Hit
ATTCTTTGACATATTTTCCAATCAATAGGTCTATTTACAGGCAGTTTCCT14990.39070135976583986No Hit
CTCTTATATAGAATGCCAGCCTTCCACAGAGTATGTAAATAATGCCTAGT12790.33336026627118687No Hit
ATCCTAACCTTACCAAATATTTGCCCTTGGATAAAGGCATTAAACCTTAT12350.3218920475722563No Hit
GGATAATAAGGTTTAATGCCTTTATCCAAGGGCAAATATTTGGTAAGGTT11790.30729613286452645No Hit
AGTCTGTACAACATCTTGAGTCCCTTTTTACCTCTATTACCAATTTTCTT11200.2919182941545968No Hit
CAATATGTTCCTGTGGTAAAGTACCCCAACTTCCAATTACATATCCCATG10750.28018943412159963No Hit
ATTTACAGGAGGACATTCTTAATAGATGTCAACAATATGTGGGCCCTCTT9690.25256145271053954No Hit
CTCCAAAAGACACCAAATACTCAAGAACAGTTTCTCTTCCGAAAGTAAGA9370.2442209300204082No Hit
GTACAACATCTTGAGTCCCTTTTTACCTCTATTACCAATTTTCTTTTGTC9070.23640168999841008No Hit
GTTTTGAAGTAATGATTAACTGCATGTTCAGGATAATAAGGTTTAATGCC9020.23509848332807706No Hit
GGTTAGGATAAAACCTAGCAGGCATGATTAATTTTAATCTCCTTTTTTCA8540.22258769929288008No Hit
GAGTATGTAAATAATGCCTAGTTTTGAAGTAATGATTAACTGCATGTTCA8510.22180577529068024No Hit
CATTTGAACCCTAATAAAACCAAACGTTGGGGCTATTCCCTTAACTTCAT8390.218678079281881No Hit
TCCACAGAGTATGTAAATAATGCCTAGTTTTGAAGTAATGATTAACTGCA8040.20955563258954987No Hit
GTGTAAAAGGGGCAGCAAAGCCTAAAAGCCCCACAATTCTTTGACATATT7950.20720986058295043No Hit
CTTTAATCCTGAGTGGCAAACTCCCTCCTTTCCTAACATCCATTTACAGG7480.19495971788182No Hit
ATATGTTCCTGTGGTAAAGTACCCCAACTTCCAATTACATATCCCATGAA7360.19183202187302076No Hit
CTGTAAGAGGGCCCACATATTGTTGACATCTATTAAGAATGTCCTCCTGT7310.19052881520268775No Hit
GTATACATGCATATAAGGGCATCAAAGCAGGATAGCCACATTGTGTAAAA7300.19026817386862113No Hit
AGTCTAGACTCTGTGGTATTGTGAGGATTCTTGTCAACAAGAAAAACCCC7230.1884436845301549No Hit
CAGTTATATGGATGATGTGGTATTGGGGGCCAAGTCTGTACAACATCTTG6720.17515097649275807No Hit
GTTATATGGATGATGTGGTATTGGGGGCCAAGTCTGTACAACATCTTGAG6590.17176263914989223No Hit
CTATATAAGAGAGAAACTACACGCAGCGCTTCATTTTTTGGGTCACCATA6580.17150199781582562No Hit
CTACTGTACCTGTCTTTAATCCTGAGTGGCAAACTCCCTCCTTTCCTAAC6550.1707200738136258No Hit
GTTTTATCCTAACCTTACCAAATATTTGCCCTTGGATAAAGGCATTAAAC6460.16837430180702637No Hit
ATGTGGTATTGGGGGCCAAGTCTGTACAACATCTTGAGTCCCTTTTTACC6450.16811366047295978No Hit
TCCTAAAACATTGCTTGATTTTGAGTACAATATGTTCCTGTGGTAAAGTA6400.16681045380262674No Hit
CATATAAGGGCATCAAAGCAGGATAGCCACATTGTGTAAAAGGGGCAGCA6400.16681045380262674No Hit
GTTCAGGATAATAAGGTTTAATGCCTTTATCCAAGGGCAAATATTTGGTA6320.16472532313009391No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN6230.16237955112349448No Hit
TAATAGATGTCAACAATATGTGGGCCCTCTTACAGTTAATGAAAAAAGGA6210.16185826845536128No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGGAGCACCCACG6160.16055506178502824No Hit
GGATAAAACCTAGCAGGCATGATTAATTTTAATCTCCTTTTTTCATTAAC6010.15664544177402917No Hit
GGTCTATTTACAGGCAGTTTCCTAAAACATTGCTTGATTTTGAGTACAAT5960.15534223510369616No Hit
GATTAACTGCATGTTCAGGATAATAAGGTTTAATGCCTTTATCCAAGGGC5930.15456031110149634No Hit
TCTTAATAGATGTCAACAATATGTGGGCCCTCTTACAGTTAATGAAAAAA5920.15429966976742973No Hit
CAATAGGTCTATTTACAGGCAGTTTCCTAAAACATTGCTTGATTTTGAGT5880.15325710443116333No Hit
CTTTTTACCTCTATTACCAATTTTCTTTTGTCTTTGGGTATACATTTGAA5880.15325710443116333No Hit
CTATTAAGAATGTCCTCCTGTAAATGGATGTTAGGAAAGGAGGGAGTTTG5780.15065069109049728No Hit
CCTCCCGCTTACAGACCACCAAATGCCCCTATCTTATCAACACTTCCGGA5730.14934748442016424No Hit
GTCTATTTACAGGCAGTTTCCTAAAACATTGCTTGATTTTGAGTACAATA5630.14674107107949821No Hit
TTCTTCTACTGTACCTGTCTTTAATCCTGAGTGGCAAACTCCCTCCTTTC5630.14674107107949821No Hit
GTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATTGCTGAGAGTCCAA5620.1464804297454316No Hit
GGGTAAAGGTGCAGATATTGTTTACACAGAAAGGCCTTGTAAGTTGGCGA5600.1459591470772984No Hit
CTTACAGTTAATGAAAAAAGGAGATTAAAATTAATCATGCCTGCTAGGTT5490.14309209240256573No Hit
ATTTACAGGCAGTTTCCTAAAACATTGCTTGATTTTGAGTACAATATGTT5420.14126760306409952No Hit
CTATTACCAATTTTCTTTTGTCTTTGGGTATACATTTGAACCCTAATAAA5340.13918247239156667No Hit
CCCTTAACTTCATGGGATATGTAATTGGAAGTTGGGGTACTTTACCACAG5310.13840054838936688No Hit
ATGATTAACTGCATGTTCAGGATAATAAGGTTTAATGCCTTTATCCAAGG5010.13058130836736875No Hit
GTATACATTTGAACCCTAATAAAACCAAACGTTGGGGCTATTCCCTTAAC5010.13058130836736875No Hit
ATGTTAATATGGGCCTAAAAATCAGACAACTATTGTGGTTTCACATTTCC4850.12641104702230307No Hit
ATATAAGAGAGAAACTACACGCAGCGCTTCATTTTTTGGGTCACCATATT4840.1261504056882365No Hit
CTTTTACACAATGTGGCTATCCTGCTTTGATGCCCTTATATGCATGTATA4790.12484719901790346No Hit
ATTTACATACTCTGTGGAAGGCTGGCATTCTATATAAGAGAGAAACTACA4780.12458655768383683No Hit
GTATCGGGAGGCCTTAGAGTCTCCGGAACATTGTTCACCTCACCATACAG4770.12432591634977024No Hit
GCCTAAAAGCCCCACAATTCTTTGACATATTTTCCAATCAATAGGTCTAT4710.12276206834537062No Hit
GTACAATATGTTCCTGTGGTAAAGTACCCCAACTTCCAATTACATATCCC4700.12250142701130402No Hit
GTGGTAAAGTACCCCAACTTCCAATTACATATCCCATGAAGTTAAGGGAA4670.1217195030091042No Hit
CTCAAGAACAGTTTCTCTTCCGAAAGTAAGACAGGAAATGTGAAACCACA4590.11963437233657136No Hit
CTCATAAGGTGGGAAACTTTACTGGGCTTTATTCTTCTACTGTACCTGTC4580.11937373100250476No Hit
TTATTATCCTGAACATGCAGTTAATCATTACTTCAAAACTAGGCATTATT4580.11937373100250476No Hit
GATTAAAATTAATCATGCCTGCTAGGTTTTATCCTAACCTTACCAAATAT4560.11885244833437156No Hit
CAATATGTGGGCCCTCTTACAGTTAATGAAAAAAGGAGATTAAAATTAAT4540.11833116566623833No Hit
GTTTAATGCCTTTATCCAAGGGCAAATATTTGGTAAGGTTAGGATAAAAC4540.11833116566623833No Hit
GTCTTTGGGTATACATTTGAACCCTAATAAAACCAAACGTTGGGGCTATT4490.11702795899590532No Hit
CTGTACAACATCTTGAGTCCCTTTTTACCTCTATTACCAATTTTCTTTTG4390.11442154565523928No Hit
GGATAGCCACATTGTGTAAAAGGGGCAGCAAAGCCTAAAAGCCCCACAAT4320.11259705631677305No Hit
CATGTATACAAGCTAAGCAGGCTTTCACTTTCTCGCCAACTTACAAGGCC4280.11155449098050664No Hit
GGGTATACATTTGAACCCTAATAAAACCAAACGTTGGGGCTATTCCCTTA4280.11155449098050664No Hit
GTTGTCAATATGCCCTGAGCCTGAGGGCTCCACCCCAAAAGACCGCCGTG4260.11103320831237344No Hit
GTGTAGTTTCTCTCTTATATAGAATGCCAGCCTTCCACAGAGTATGTAAA4260.11103320831237344No Hit
ATCTCAATGTTAGTATCCCTTGGACTCATAAGGTGGGAAACTTTACTGGG4230.11025128431017361No Hit
GGAACATATTGTACTCAAAATCAAGCAATGTTTTAGGAAACTGCCTGTAA4200.1094693603079738No Hit
ACATTGACCCGTATAAAGAATTTGGAGCTTCTGTGGAGTTACTCTCTTTT4140.10790551230357417No Hit
CCTTTATCCAAGGGCAAATATTTGGTAAGGTTAGGATAAAACCTAGCAGG4130.10764487096950758No Hit
AACATATTGTACTCAAAATCAAGCAATGTTTTAGGAAACTGCCTGTAAAT4130.10764487096950758No Hit
AAATTAATCATGCCTGCTAGGTTTTATCCTAACCTTACCAAATATTTGCC4110.10712358830137435No Hit
GAAAAAAGGAGATTAAAATTAATCATGCCTGCTAGGTTTTATCCTAACCT4090.10660230563324115No Hit
ATGTAAATAATGCCTAGTTTTGAAGTAATGATTAACTGCATGTTCAGGAT4070.10608102296510795No Hit
GAATATGGTGACCCAAAAAATGAAGCGCTGCGTGTAGTTTCTCTCTTATA4070.10608102296510795No Hit
GTTCAAATGTATACCCAAAGACAAAAGAAAATTGGTAATAGAGGTAAAAA4040.10529909896290814No Hit
GTATTGGGGGCCAAGTCTGTACAACATCTTGAGTCCCTTTTTACCTCTAT4030.10503845762884154No Hit
ACAATATGTGGGCCCTCTTACAGTTAATGAAAAAAGGAGATTAAAATTAA4020.10477781629477494No Hit
CATTCTTAATAGATGTCAACAATATGTGGGCCCTCTTACAGTTAATGAAA4010.10451717496070831No Hit
GCCTTAGAGTCTCCGGAACATTGTTCACCTCACCATACAGCACTCAGGCA4010.10451717496070831No Hit
GTATAAAGAATTTGGAGCTTCTGTGGAGTTACTCTCTTTTTTGCCTTCTG4010.10451717496070831No Hit
GAATTAGTAGTCAGCTATGTCAATGTTAATATGGGCCTAAAAATCAGACA3880.10112883761784247No Hit
TCTGTGGAGTTACTCTCTTTTTTGCCTTCTGACTTCTTTCCTTCTATTCG3880.10112883761784247No Hit
CCACAATAGTTGTCTGATTTTTAGGCCCATATTAACATTGACATAGCTGA3860.10060755494970926No Hit
ATTGACAACAGTGCCAGCAGCGCCTCCTCCTGCCTCCACCAATCGGCAGT3850.10034691361564266No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)