FastQCFastQC Report
Sat 3 Sep 2022
EGAF00002490275

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002490275
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences383669
Sequences flagged as poor quality0
Sequence length35-151
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATGTAAATAATGCCTAGTTTTGAAGTAATGATTAACTGCATGTTCAGG32660.8512545970615296No Hit
CTTATATAGAATGCCAGCCTTCCACAGAGTATGTAAATAATGCCTAGTTT20070.5231071574716748No Hit
CTCTTACAGTTAATGAAAAAAGGAGATTAAAATTAATCATGCCTGCTAGG19720.5139847107793437No Hit
ATTCTTTGACATATTTTCCAATCAATAGGTCTATTTACAGGCAGTTTCCT15810.41207394915930134No Hit
TTCTTAATAGATGTCAACAATATGTGGGCCCTCTTACAGTTAATGAAAAA13690.35681798633718126No Hit
CTCTTATATAGAATGCCAGCCTTCCACAGAGTATGTAAATAATGCCTAGT13160.34300399563165124No Hit
GGATAATAAGGTTTAATGCCTTTATCCAAGGGCAAATATTTGGTAAGGTT12030.31355152488212495No Hit
ATCCTAACCTTACCAAATATTTGCCCTTGGATAAAGGCATTAAACCTTAT10850.28279584746226566No Hit
AGTCTGTACAACATCTTGAGTCCCTTTTTACCTCTATTACCAATTTTCTT10660.27784366211500017No Hit
CAATATGTTCCTGTGGTAAAGTACCCCAACTTCCAATTACATATCCCATG10170.26507223674573654No Hit
GAGTATGTAAATAATGCCTAGTTTTGAAGTAATGATTAACTGCATGTTCA9700.25282209404460615No Hit
CTCCAAAAGACACCAAATACTCAAGAACAGTTTCTCTTCCGAAAGTAAGA9560.24917311536767367No Hit
GTTTTGAAGTAATGATTAACTGCATGTTCAGGATAATAAGGTTTAATGCC9490.24734862602920749No Hit
ATTTACAGGAGGACATTCTTAATAGATGTCAACAATATGTGGGCCCTCTT9350.243699647352275No Hit
GTACAACATCTTGAGTCCCTTTTTACCTCTATTACCAATTTTCTTTTGTC9170.23900810333907613No Hit
GGTTAGGATAAAACCTAGCAGGCATGATTAATTTTAATCTCCTTTTTTCA8530.22232705795881347No Hit
CATTTGAACCCTAATAAAACCAAACGTTGGGGCTATTCCCTTAACTTCAT7980.20799178458515022No Hit
TCCACAGAGTATGTAAATAATGCCTAGTTTTGAAGTAATGATTAACTGCA7560.1970448485543528No Hit
GTGTAAAAGGGGCAGCAAAGCCTAAAAGCCCCACAATTCTTTGACATATT7490.1952203592158866No Hit
CTGTAAGAGGGCCCACATATTGTTGACATCTATTAAGAATGTCCTCCTGT7160.18661919519168868No Hit
GTATACATGCATATAAGGGCATCAAAGCAGGATAGCCACATTGTGTAAAA7100.18505534718728903No Hit
GTTTTATCCTAACCTTACCAAATATTTGCCCTTGGATAAAGGCATTAAAC7000.182448933846623No Hit
ATATGTTCCTGTGGTAAAGTACCCCAACTTCCAATTACATATCCCATGAA6670.17384776982242506No Hit
CTTTAATCCTGAGTGGCAAACTCCCTCCTTTCCTAACATCCATTTACAGG6540.1704594324795592No Hit
CAGTTATATGGATGATGTGGTATTGGGGGCCAAGTCTGTACAACATCTTG6530.1701987911454926No Hit
GGATAAAACCTAGCAGGCATGATTAATTTTAATCTCCTTTTTTCATTAAC6390.16654981246856013No Hit
GTTCAGGATAATAAGGTTTAATGCCTTTATCCAAGGGCAAATATTTGGTA6380.16628917113449354No Hit
CTTTTTACCTCTATTACCAATTTTCTTTTGTCTTTGGGTATACATTTGAA6350.1655072471322937No Hit
AGTCTAGACTCTGTGGTATTGTGAGGATTCTTGTCAACAAGAAAAACCCC6230.16237955112349448No Hit
GATTAACTGCATGTTCAGGATAATAAGGTTTAATGCCTTTATCCAAGGGC6190.16133698578722805No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN6170.16081570311909485No Hit
GTCTATTTACAGGCAGTTTCCTAAAACATTGCTTGATTTTGAGTACAATA6160.16055506178502824No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGGAGCACCCACG6120.15951249644876184No Hit
ATGTGGTATTGGGGGCCAAGTCTGTACAACATCTTGAGTCCCTTTTTACC5990.15612415910589597No Hit
CTATTAAGAATGTCCTCCTGTAAATGGATGTTAGGAAAGGAGGGAGTTTG5970.15560287643776277No Hit
CTACTGTACCTGTCTTTAATCCTGAGTGGCAAACTCCCTCCTTTCCTAAC5910.15403902843336312No Hit
GTTATATGGATGATGTGGTATTGGGGGCCAAGTCTGTACAACATCTTGAG5880.15325710443116333No Hit
CCTCCCGCTTACAGACCACCAAATGCCCCTATCTTATCAACACTTCCGGA5840.1522145390948969No Hit
CAATAGGTCTATTTACAGGCAGTTTCCTAAAACATTGCTTGATTTTGAGT5790.1509113324245639No Hit
CTATATAAGAGAGAAACTACACGCAGCGCTTCATTTTTTGGGTCACCATA5740.14960812575423088No Hit
CTATTACCAATTTTCTTTTGTCTTTGGGTATACATTTGAACCCTAATAAA5730.14934748442016424No Hit
TCCTAAAACATTGCTTGATTTTGAGTACAATATGTTCCTGTGGTAAAGTA5570.1451772230750986No Hit
ATTTACAGGCAGTTTCCTAAAACATTGCTTGATTTTGAGTACAATATGTT5540.14439529907289878No Hit
CTTACAGTTAATGAAAAAAGGAGATTAAAATTAATCATGCCTGCTAGGTT5500.14335273373663238No Hit
CCCTTAACTTCATGGGATATGTAATTGGAAGTTGGGGTACTTTACCACAG5440.14178888573223272No Hit
GGTCTATTTACAGGCAGTTTCCTAAAACATTGCTTGATTTTGAGTACAAT5400.14074632039596632No Hit
GGGTAAAGGTGCAGATATTGTTTACACAGAAAGGCCTTGTAAGTTGGCGA5370.1399643963937665No Hit
TAATAGATGTCAACAATATGTGGGCCCTCTTACAGTTAATGAAAAAAGGA5320.1386611897234335No Hit
CATATAAGGGCATCAAAGCAGGATAGCCACATTGTGTAAAAGGGGCAGCA5310.13840054838936688No Hit
GTATACATTTGAACCCTAATAAAACCAAACGTTGGGGCTATTCCCTTAAC5290.13787926572123366No Hit
TTCTTCTACTGTACCTGTCTTTAATCCTGAGTGGCAAACTCCCTCCTTTC5240.13657605905090064No Hit
ATGTTAATATGGGCCTAAAAATCAGACAACTATTGTGGTTTCACATTTCC5190.13527285238056763No Hit
GTCTTTGGGTATACATTTGAACCCTAATAAAACCAAACGTTGGGGCTATT5070.13214515637176838No Hit
GTACAATATGTTCCTGTGGTAAAGTACCCCAACTTCCAATTACATATCCC5050.13162387370363515No Hit
GTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATTGCTGAGAGTCCAA5020.13084194970143537No Hit
GTATAAAGAATTTGGAGCTTCTGTGGAGTTACTCTCTTTTTTGCCTTCTG4910.1279748950267027No Hit
CTCAAGAACAGTTTCTCTTCCGAAAGTAAGACAGGAAATGTGAAACCACA4840.1261504056882365No Hit
GGAACATATTGTACTCAAAATCAAGCAATGTTTTAGGAAACTGCCTGTAA4780.12458655768383683No Hit
ATTTACATACTCTGTGGAAGGCTGGCATTCTATATAAGAGAGAAACTACA4710.12276206834537062No Hit
GTATCGGGAGGCCTTAGAGTCTCCGGAACATTGTTCACCTCACCATACAG4700.12250142701130402No Hit
ATATAAGAGAGAAACTACACGCAGCGCTTCATTTTTTGGGTCACCATATT4690.12224078567723742No Hit
ATGATTAACTGCATGTTCAGGATAATAAGGTTTAATGCCTTTATCCAAGG4690.12224078567723742No Hit
CTTTTACACAATGTGGCTATCCTGCTTTGATGCCCTTATATGCATGTATA4690.12224078567723742No Hit
TCTTAATAGATGTCAACAATATGTGGGCCCTCTTACAGTTAATGAAAAAA4610.12015565500470458No Hit
ATCTCAATGTTAGTATCCCTTGGACTCATAAGGTGGGAAACTTTACTGGG4600.11989501367063798No Hit
GGGTATACATTTGAACCCTAATAAAACCAAACGTTGGGGCTATTCCCTTA4570.11911308966843816No Hit
GCCTAAAAGCCCCACAATTCTTTGACATATTTTCCAATCAATAGGTCTAT4560.11885244833437156No Hit
CTGTACAACATCTTGAGTCCCTTTTTACCTCTATTACCAATTTTCTTTTG4540.11833116566623833No Hit
CCTTTATCCAAGGGCAAATATTTGGTAAGGTTAGGATAAAACCTAGCAGG4510.11754924166403853No Hit
ATGTAAATAATGCCTAGTTTTGAAGTAATGATTAACTGCATGTTCAGGAT4500.11728860032997193No Hit
GTGTAGTTTCTCTCTTATATAGAATGCCAGCCTTCCACAGAGTATGTAAA4390.11442154565523928No Hit
GTTTAATGCCTTTATCCAAGGGCAAATATTTGGTAAGGTTAGGATAAAAC4320.11259705631677305No Hit
CAATATGTGGGCCCTCTTACAGTTAATGAAAAAAGGAGATTAAAATTAAT4290.11181513231457324No Hit
TTATTATCCTGAACATGCAGTTAATCATTACTTCAAAACTAGGCATTATT4250.11077256697830681No Hit
GTCTTACATAAGAGGACTCTTGGACTCTCAGCAATGTCAACGACCGACCT4220.10999064297610701No Hit
GCCTTAGAGTCTCCGGAACATTGTTCACCTCACCATACAGCACTCAGGCA4180.1089480776398406No Hit
GTATTGGGGGCCAAGTCTGTACAACATCTTGAGTCCCTTTTTACCTCTAT4160.10842679497170737No Hit
GTGGTAAAGTACCCCAACTTCCAATTACATATCCCATGAAGTTAAGGGAA4070.10608102296510795No Hit
GAATATGGTGACCCAAAAAATGAAGCGCTGCGTGTAGTTTCTCTCTTATA4010.10451717496070831No Hit
ATAATAAGGTTTAATGCCTTTATCCAAGGGCAAATATTTGGTAAGGTTAG4000.10425653362664171No Hit
GCTTGTATACATGCATATAAGGGCATCAAAGCAGGATAGCCACATTGTGT3980.10373525095850851No Hit
AACATATTGTACTCAAAATCAAGCAATGTTTTAGGAAACTGCCTGTAAAT3970.1034746096244419No Hit
GATTAAAATTAATCATGCCTGCTAGGTTTTATCCTAACCTTACCAAATAT3960.1032139682903753No Hit
GAATTAGTAGTCAGCTATGTCAATGTTAATATGGGCCTAAAAATCAGACA3920.10217140295410887No Hit
ATATGTGGGCCCTCTTACAGTTAATGAAAAAAGGAGATTAAAATTAATCA3910.10191076162004227No Hit
CTCATAAGGTGGGAAACTTTACTGGGCTTTATTCTTCTACTGTACCTGTC3890.10138947895190907No Hit
CTTTTGGAGTGTGGATTCGCACTCCTCCCGCTTACAGACCACCAAATGCC3890.10138947895190907No Hit
CCTTTACCCCGTTGCCCGGCAACGGTCAGGTCTCTGCCAAGTGTTTGCTG3860.10060755494970926No Hit
ACATTGACCCGTATAAAGAATTTGGAGCTTCTGTGGAGTTACTCTCTTTT3860.10060755494970926No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)