FastQCFastQC Report
Sat 3 Sep 2022
EGAF00002490276

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002490276
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences440018
Sequences flagged as poor quality0
Sequence length35-151
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATTGCTGAGAGTCCAA13600.309078264980069No Hit
ATGTAAGACCTTGGGCAAGACCTGGTGGGCGTTCACGGTGGTCTCCATGC12680.28817002940788783No Hit
GTATACATGCATATAAAGGCATCAAAGCAGGATAGCCACATTGTGTAAAA12390.28157938993404813No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGGAGCACCCACG11580.2631710520933235No Hit
CTTATATAGAATGCCAGCCTTCCACAGAGTATGTAAATAATGTCTAGTTT11490.2611256812221318No Hit
CATGTATACAAGCCAAGCAGGCTTTCACTTTCTCGCCAACTTACAAGGCC11460.2604438909317346No Hit
GTATGTAAATAATGTCTAGTTTTGAAGTAATGATTAACTGCATGTTCAGG11400.2590803103509402No Hit
GTGTAAAAGGGGCAGCAAAGCCCAAAAGCCCCACAATTCTTTGACATACT10300.2340813330363758No Hit
CTATATAAGAGAGAAACTACACGCAGCGCTTCATTTTGTGGGTCACCATA10270.23339954274597857No Hit
CCTACTCTCCTCCCCCAACTCCTCCCAGTCCTTAAACAAACAGTCTTTGA10230.23249048902544894No Hit
GTGCTGGTGAACAGACCAATTTATGCCTACAGCCTCCTATTACAAAGATC9650.21930921007776955No Hit
CTCCAAAAGACACCAAATACTCAAGAACAGTTTCTCTTCCGAAAGTAAGA9170.2084005654314142No Hit
GTATCGGGAGGCCTTAGAGTCTCCGGAACATTGTTCACCTCACCATACAG8840.20090087223704486No Hit
TCCCATGGCTGCTCGGGTGTGCTGCCAACTGGATCCTGCGAGGGACGTCC8570.19476475962346995No Hit
CCTACCGCTTACAGACCACCAAATGCCCCTATCTTATCAACACTTCCGGA8470.19249212532214593No Hit
TCTGTGGAGTTACTCTCTTTTTTGCCTTCTGATTTCTTTCCTTCTATTCG8450.1920375984618811No Hit
CCTTTACCCCGTTGCCCGGCAACGGTCTGGTCTCTGCCAAGTGTTTGCTG8450.1920375984618811No Hit
GCCATGGGAAGGAGGTGTATTTCCGAGAGAGGACAACAGAGTTGTCAGTT8260.18771959328936544No Hit
GACTTCGCCCCCAATACCACATCATCCATATAACTGAAAGCCAAACAGTG8250.18749232985923303No Hit
ATATGTTCCTGTGGTAAAGTACCCCAACTTCCAATTACATATCCCATGAA8190.18612874927843862No Hit
CTCTTACAGTTAATGAAAAAAGGAGATTAAAATTAATTATGCCTGCTAGG7640.1736292606211564No Hit
GTAGAGGCCCAAACGGCCCCGAGACGGGTCGTCCGCGGGATTCAGCGCCG7560.17181115318009718No Hit
GTCCGGCAGATGAGAAGGCACAGACGGGGAGACCGCGTAAAGAGAGGTAC7520.17090209945956758No Hit
AGTCTAGACTCTGTGGTATTGTGAGGATTCTTGTCAACAAGAAAAACCCC7430.16885672858837594No Hit
CTCCCATAGGAATCTTGCGAAAGCCCAGGACGATGGGATGGGAATACAAG7380.1677204114377139No Hit
CTTTTGGAGTGTGGATTCGCACTCCTACCGCTTACAGACCACCAAATGCC7280.16544777713638986No Hit
ATATAAGAGAGAAACTACACGCAGCGCTTCATTTTGTGGGTCACCATATT7210.16385693312546307No Hit
CTCCTACCGCTTACAGACCACCAAATGCCCCTATCTTATCAACACTTCCG7180.16317514283506585No Hit
CTTTTACACAATGTGGCTATCCTGCTTTGATGCCTTTATATGCATGTATA7150.16249335254466862No Hit
CATATAAAGGCATCAAAGCAGGATAGCCACATTGTGTAAAAGGGGCAGCA7060.16044798167347699No Hit
GTCCAAGAGTCCTCTTATGTAAGACCTTGGGCAAGACCTGGTGGGCGTTC7040.15999345481321217No Hit
CTAGTAAACTGAGCCAGGAGAAACGGACTGAGGCCCACTCCCATAGGAAT6990.15885713766255016No Hit
GCCTTAGAGTCTCCGGAACATTGTTCACCTCACCATACAGCACTCAGGCA6940.15772082051188815No Hit
TCCCCTCGGCGCTGAATCCCGCGGACGACCCGTCTCGGGGCCGTTTGGGC6890.15658450336122615No Hit
GCACGGGACCATGCAAGACATGCACGACTCCTGCTCAAGGAACCTCTATG6880.1563572399310937No Hit
CATTTGAACCCTAATAAAACCAAGCGTTGGGGCTATTCCCTTAACTTCAT6800.1545391324900345No Hit
GGGTAAAGGTGCAGATATTGTTGACACAGAAAGGCCTTGTAAGTTGGCGA6770.1538573421996373No Hit
GTATAAAGAATTTGGAGCTTCTGTGGAGTTACTCTCTTTTTTGCCTTCTG6710.15249376161884287No Hit
GTGCAGAGGTGAAGCGAAGTGCACACGGTCCGGCAGATGAGAAGGCACAG6710.15249376161884287No Hit
GTATGGATCGGCAGAGGAGCCACAAAGGTTCCACGCATGCGCCGATGGCC6640.15090291760791605No Hit
GTACAATATGTTCCTGTGGTAAAGTACCCCAACTTCCAATTACATATCCC6480.1472667027257976No Hit
GTTGTCAATATGCCCTGAGCCTGAGGGCTCCACCCCAAAAGACCGCCGTG6370.14476680499434114No Hit
ACATTGACCCGTATAAAGAATTTGGAGCTTCTGTGGAGTTACTCTCTTTT6320.14363048784367913No Hit
GGATAGCCACATTGTGTAAAAGGGGCAGCAAAGCCCAAAAGCCCCACAAT6310.14340322441354672No Hit
GGGCCAAGTCTGTACAACATCTTGAGTCCCTTTTTACCTCTATTACCAAT6160.13999427296156067No Hit
CTGTAAGAGGGCCCACATATTGTTGACATCTATTAAGAATGTCCTCCTGT6110.13885795581089863No Hit
TTCTTAATAGATGTCAACAATATGTGGGCCCTCTTACAGTTAATGAAAAA6080.13817616552050144No Hit
CCTCTATGTTTCCCTCTTGTTGCTGTACAAAACCTTCGGATGGAAACTGC6060.13772163866023662No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN6060.13772163866023662No Hit
ATCCTAACCTTACCAAATATTTGCCCTTGGATAAAGGCATTAAACCTTAT6050.13749437523010422No Hit
GGTTAGGATAAAACCTAGCAGGCATAATTAATTTTAATCTCCTTTTTTCA6020.136812584939707No Hit
CTATTAAGAATGTCCTCCTGTAAATGAATGTTAGGAAAGGAGGGAGTTTG6010.13658532150957461No Hit
GACGGGGAGACCGCGTAAAGAGAGGTACGCCCCGTGGTCGGCCGGAACGG5990.1361307946493098No Hit
TAATAAAACCAAGCGTTGGGGCTATTCCCTTAACTTCATGGGATATGTAA5940.1349944774986478No Hit
GTCGTTGACATTGCTGAGAGTCCAAGAGTCCTCTTATGTAAGACCTTGGG5910.13431268720825057No Hit
GTTTTATTAGGGTTCAAATGTATACCCAAAGACAAAAGAAAATTGGTAAT5870.13340363348772094No Hit
ATACCACATCATCCATATAACTGAAAGCCAAACAGTGGGGGAAAGCCCTA5850.13294910662745615No Hit
CTCTTATATAGAATGCCAGCCTTCCACAGAGTATGTAAATAATGTCTAGT5750.1306764723261321No Hit
GGATAATAAGGTTTAATGCCTTTATCCAAGGGCAAATATTTGGTAAGGTT5660.12863110145494047No Hit
CCCTTAACTTCATGGGATATGTAATTGGAAGTTGGGGTACTTTACCACAG5630.12794931116454328No Hit
GAATACAAGTGCAGTTTCCATCCGAAGGTTTTGTACAGCAACAAGAGGGA5610.12749478430427846No Hit
ATGTTAATATGGGCCTAAAACTCAGACAAATATTGTGGTTTCACATTTCC5600.12726752087414606No Hit
GCTCTGTATCGGGAGGCCTTAGAGTCTCCGGAACATTGTTCACCTCACCA5570.12658573058374883No Hit
CGTAAAGAGAGGTACGCCCCGTGGTCGGCCGGAACGGCAGATGAAGAAGG5560.12635846715361645No Hit
TCACCATACAGCACTCAGGCAAGCTATTCTGTGTTGGGGTGAGTTGATGA5510.12522215000295442No Hit
CCCCCAACTCCTCCCAGTCCTTAAACAAACAGTCTTTGAAGTATGCCTCA5490.12476762314268962No Hit
TATTAACATTGACATAGCTGACTACTAATTCCCTGGATGCTGGATCTTCC5470.12431309628242482No Hit
ACATAGAGGTTCCTTGAGCAGGAGTCGTGCATGTCTTGCATGGTCCCGTG5400.12272225227149798No Hit
ATTGACAACAGTGCCAGCAGCGCCTCCTCCTGCCTCCACCAATCGGCAGT5380.12226772541123318No Hit
CTTGTATTCCCATCCCATCGTCCTGGGCTTTCGCAAGATTCCTATGGGAG5350.12158593512083596No Hit
CCTTATGAGTCCAGGGGATACTAACATTGAGATTCCCGAGATTGAGATCT5350.12158593512083596No Hit
GTCTTACATAAGAGGACTCTTGGACTCTCAGCAATGTCAACGACCGACCT5340.12135867169070357No Hit
CTCTTTACGCGGTCTCCCCGTCTGTGCCTTCTCATCTGCCGGACCGTGTG5310.12067688140030636No Hit
GGCCTCTACCGTCCCCTTCTTCATCTGCCGTTCCGGCCGACCACGGGGCG5230.11885877395924713No Hit
GCCTAATCATCTCATGTTCATGTCCTACTGTTCAAGCCTCCAAGCTGTGC5180.1177224568085851No Hit
CTTTAATCCTGAGTGGCAAACTCCCTCCTTTCCTAACATTCATTTACAGG5170.1174951933784527No Hit
ATTTACATACTCTGTGGAAGGCTGGCATTCTATATAAGAGAGAAACTACA5170.1174951933784527No Hit
ATACAGCACTCAGGCAAGCTATTCTGTGTTGGGGTGAGTTGATGAATCTG5140.1168134030880555No Hit
CCTCAAGGTCGGTCGTTGACATTGCTGAGAGTCCAAGAGTCCTCTTATGT5130.11658613965792308No Hit
GCCATAGGCCATCGGCGCATGCGTGGAACCTTTGTGGCTCCTCTGCCGAT5120.11635887622779068No Hit
GACTTGGCCCCCAATACCACATCATCCATATAACTGAAAGCCAAACAGTG5120.11635887622779068No Hit
GGATTAAAGACAGGTACAGTAGAAGAATAAAGCCCAGTAAAGTTTCCCAC5110.11613161279765828No Hit
AGTCTGTACAACATCTTGAGTCCCTTTTTACCTCTATTACCAATTTTCTT5090.11567708593739348No Hit
CCCATAGGAATCTTGCGAAAGCCCAGGACGATGGGATGGGAATACAAGTG5090.11567708593739348No Hit
GTCCCGTGCTGGTAGTTGATGTTCCTGGAAGTAGAGGACAAACGGGCAAC5010.11385897849633424No Hit
GATCCATACTGCGGAACTCCTAGCAGCTTGTTTTGCTCGCAGCCGGTCTG5000.11363171506620184No Hit
GTGTTGGGGTGAGTTGATGAATCTGGCCACCTGGGTGGGAAGTAATTTGG4970.11294992477580462No Hit
CTACTGTACCTGTCTTTAATCCTGAGTGGCAAACTCCCTCCTTTCCTAAC4940.1122681344854074No Hit
GTTTACTAGTGCCATTTGTTCAGTGGTTCGTAGGGCTTTCCCCCACTGTT4820.10954097332381857No Hit
GTGGTAAAGTACCCCAACTTCCAATTACATATCCCATGAAGTTAAGGGAA4820.10954097332381857No Hit
CCGATGGGACGTAGACAAAGGACGTCCCTCGCAGGATCCAGTTGGCAGCA4740.10772286588275934No Hit
GTTCAAATGTATACCCAAAGACAAAAGAAAATTGGTAATAGAGGTAAAAA4730.10749560245262695No Hit
GTGCACACGGTCCGGCAGATGAGAAGGCACAGACGGGGAGACCGCGTAAA4720.10726833902249452No Hit
TCCCGATACAGAGCAGAGGCGGTGTCGAGGAGATCTCGAATAGAAGGAAA4720.10726833902249452No Hit
GGCTTTCACTTTCTCGCCAACTTACAAGGCCTTTCTGTGTCAACAATATC4700.10681381216222972No Hit
GGCTGGGGCTTGGCCATAGGCCATCGGCGCATGCGTGGAACCTTTGTGGC4700.10681381216222972No Hit
CTCATAAGGTGGGAAACTTTACTGGGCTTTATTCTTCTACTGTACCTGTC4690.10658654873209733No Hit
GTTCAAGCCTCCAAGCTGTGCCTTGGGTGGCTTTGGGGCATGGACATTGA4690.10658654873209733No Hit
GAGTATGTAAATAATGTCTAGTTTTGAAGTAATGATTAACTGCATGTTCA4680.10635928530196492No Hit
CCCCTATCTTATCAACACTTCCGGAAACTACTGTTGTTAGACGACGAGGC4660.10590475844170011No Hit
GTACAACATCTTGAGTCCCTTTTTACCTCTATTACCAATTTTCTTTTGTC4650.1056774950115677No Hit
CTCTAAGGCCTCCCGATACAGAGCAGAGGCGGTGTCGAGGAGATCTCGAA4650.1056774950115677No Hit
CTCCATGTTCGGTGCAGGGTCCCCAGTCCTCGAGAAGATTGACGATATGG4640.10545023158143531No Hit
CTCTTATGTAAGACCTTGGGCAAGACCTGGTGGGCGTTCACGGTGGTCTC4630.1052229681513029No Hit
GCCTATGGCCAAGCCCCAGCCAGTGGGGGTTGCGTCAGCAAACACTTGGC4610.10476844129103809No Hit
TTGACATACTTTCCAATCAATAGGTCTATTTACAGGCAGTTTCCGAAAAC4600.10454117786090569No Hit
ATATAACTGAAAGCCAAACAGTGGGGGAAAGCCCTACGAACCACTGAACA4590.10431391443077329No Hit
GGAACATATTGTACTAAAACTCAAGCAATGTTTTCGGAAACTGCCTGTAA4570.10385938757050849No Hit
GTCCAGGGGATACTAACATTGAGATTCCCGAGATTGAGATCTTCTGCGAC4560.10363212414037606No Hit
GCTTTGGGGCATGGACATTGACCCGTATAAAGAATTTGGAGCTTCTGTGG4530.10295033384997887No Hit
CTATTACCAATTTTCTTTTGTCTTTGGGTATACATTTGAACCCTAATAAA4530.10295033384997887No Hit
GTACAAAACCTTCGGATGGAAACTGCACTTGTATTCCCATCCCATCGTCC4500.10226854355958165No Hit
ATGCAAGACATGCACGACTCCTGCTCAAGGAACCTCTATGTTTCCCTCTT4500.10226854355958165No Hit
GCCCAGGACGATGGGATGGGAATACAAGTGCAGTTTCCATCCGAAGGTTT4480.10181401669931685No Hit
GGTGAAGCGAAGTGCACACGGTCCGGCAGATGAGAAGGCACAGACGGGGA4460.10135948983905205No Hit
ATTCTGTGTTGGGGTGAGTTGATGAATCTGGCCACCTGGGTGGGAAGTAA4460.10135948983905205No Hit
GTGGGGGAAAGCCCTACGAACCACTGAACAAATGGCACTAGTAAACTGAG4440.10090496297878723No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)