FastQCFastQC Report
Sat 3 Sep 2022
EGAF00002490277

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002490277
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences440018
Sequences flagged as poor quality0
Sequence length35-151
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATACATGCATATAAAGGCATCAAAGCAGGATAGCCACATTGTGTAAAA13380.3040784695171561No Hit
GTATGTAAATAATGTCTAGTTTTGAAGTAATGATTAACTGCATGTTCAGG12580.2858973951065638No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGGAGCACCCACG12560.285442868246299No Hit
GTGTAAAAGGGGCAGCAAAGCCCAAAAGCCCCACAATTCTTTGACATACT12520.2845338145257694No Hit
GTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATTGCTGAGAGTCCAA11960.2718070624383548No Hit
TCCCATGGCTGCTCGGGTGTGCTGCCAACTGGATCCTGCGAGGGACGTCC11390.2588530469208078No Hit
CATGTATACAAGCCAAGCAGGCTTTCACTTTCTCGCCAACTTACAAGGCC11040.25089882686617365No Hit
ATGTAAGACCTTGGGCAAGACCTGGTGGGCGTTCACGGTGGTCTCCATGC10790.24521724111286358No Hit
GTGCTGGTGAACAGACCAATTTATGCCTACAGCCTCCTATTACAAAGATC10550.23976291878968586No Hit
CTATATAAGAGAGAAACTACACGCAGCGCTTCATTTTGTGGGTCACCATA10240.23271775245558135No Hit
CTTATATAGAATGCCAGCCTTCCACAGAGTATGTAAATAATGTCTAGTTT9760.22180910780922597No Hit
GTATCGGGAGGCCTTAGAGTCTCCGGAACATTGTTCACCTCACCATACAG9660.21953647350790192No Hit
CCTTTACCCCGTTGCCCGGCAACGGTCTGGTCTCTGCCAAGTGTTTGCTG8800.1999918185165152No Hit
CTCCAAAAGACACCAAATACTCAAGAACAGTTTCTCTTCCGAAAGTAAGA8750.19885550136585323No Hit
TCTGTGGAGTTACTCTCTTTTTTGCCTTCTGATTTCTTTCCTTCTATTCG8290.18840138357976266No Hit
CCTACCGCTTACAGACCACCAAATGCCCCTATCTTATCAACACTTCCGGA8260.18771959328936544No Hit
CTTTTGGAGTGTGGATTCGCACTCCTACCGCTTACAGACCACCAAATGCC8040.18271979782645256No Hit
ATATAAGAGAGAAACTACACGCAGCGCTTCATTTTGTGGGTCACCATATT7960.18090169038539333No Hit
GCCTTAGAGTCTCCGGAACATTGTTCACCTCACCATACAGCACTCAGGCA7830.17794726579367207No Hit
CTTTTACACAATGTGGCTATCCTGCTTTGATGCCTTTATATGCATGTATA7650.17385652405128882No Hit
CTCCTACCGCTTACAGACCACCAAATGCCCCTATCTTATCAACACTTCCG7490.17022030916917036No Hit
CTCTTACAGTTAATGAAAAAAGGAGATTAAAATTAATTATGCCTGCTAGG7440.16908399201850832No Hit
GACTTCGCCCCCAATACCACATCATCCATATAACTGAAAGCCAAACAGTG7400.16817493829797872No Hit
ATATGTTCCTGTGGTAAAGTACCCCAACTTCCAATTACATATCCCATGAA7390.1679476748678463No Hit
GTATAAAGAATTTGGAGCTTCTGTGGAGTTACTCTCTTTTTTGCCTTCTG7370.1674931480075815No Hit
CTCCCATAGGAATCTTGCGAAAGCCCAGGACGATGGGATGGGAATACAAG7350.16703862114731668No Hit
AGTCTAGACTCTGTGGTATTGTGAGGATTCTTGTCAACAAGAAAAACCCC7270.16522051370625748No Hit
CATTTGAACCCTAATAAAACCAAGCGTTGGGGCTATTCCCTTAACTTCAT7150.16249335254466862No Hit
CTGTAAGAGGGCCCACATATTGTTGACATCTATTAAGAATGTCCTCCTGT7110.16158429882413902No Hit
TCCCCTCGGCGCTGAATCCCGCGGACGACCCGTCTCGGGGCCGTTTGGGC7060.16044798167347699No Hit
GTAGAGGCCCAAACGGCCCCGAGACGGGTCGTCCGCGGGATTCAGCGCCG6910.15703903022149093No Hit
GCCATGGGAAGGAGGTGTATTTCCGAGAGAGGACAACAGAGTTGTCAGTT6810.1547663959201669No Hit
GTCCAAGAGTCCTCTTATGTAAGACCTTGGGCAAGACCTGGTGGGCGTTC6800.1545391324900345No Hit
GTCCGGCAGATGAGAAGGCACAGACGGGGAGACCGCGTAAAGAGAGGTAC6780.1540846056297697No Hit
CATATAAAGGCATCAAAGCAGGATAGCCACATTGTGTAAAAGGGGCAGCA6680.15181197132844565No Hit
CCTACTCTCCTCCCCCAACTCCTCCCAGTCCTTAAACAAACAGTCTTTGA6650.15113018103804843No Hit
GTATGGATCGGCAGAGGAGCCACAAAGGTTCCACGCATGCGCCGATGGCC6440.14635764900526796No Hit
GCACGGGACCATGCAAGACATGCACGACTCCTGCTCAAGGAACCTCTATG6420.14590312214500317No Hit
GGATAGCCACATTGTGTAAAAGGGGCAGCAAAGCCCAAAAGCCCCACAAT6290.1429486975532819No Hit
CTCTTATATAGAATGCCAGCCTTCCACAGAGTATGTAAATAATGTCTAGT6220.14135785354235508No Hit
CCTCTATGTTTCCCTCTTGTTGCTGTACAAAACCTTCGGATGGAAACTGC6200.14090332668209027No Hit
GGGCCAAGTCTGTACAACATCTTGAGTCCCTTTTTACCTCTATTACCAAT6140.13953974610129585No Hit
GGGTAAAGGTGCAGATATTGTTGACACAGAAAGGCCTTGTAAGTTGGCGA6080.13817616552050144No Hit
CTAGTAAACTGAGCCAGGAGAAACGGACTGAGGCCCACTCCCATAGGAAT6060.13772163866023662No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN6030.13703984836983943No Hit
GGTTAGGATAAAACCTAGCAGGCATAATTAATTTTAATCTCCTTTTTTCA5970.135676267789045No Hit
CTCTTTACGCGGTCTCCCCGTCTGTGCCTTCTCATCTGCCGGACCGTGTG5900.13408542377811816No Hit
GTTTTATTAGGGTTCAAATGTATACCCAAAGACAAAAGAAAATTGGTAAT5870.13340363348772094No Hit
GTACAATATGTTCCTGTGGTAAAGTACCCCAACTTCCAATTACATATCCC5820.13226731633705893No Hit
ATGTTAATATGGGCCTAAAACTCAGACAAATATTGTGGTTTCACATTTCC5820.13226731633705893No Hit
CTATTAAGAATGTCCTCCTGTAAATGAATGTTAGGAAAGGAGGGAGTTTG5770.13113099918639692No Hit
TTCTTAATAGATGTCAACAATATGTGGGCCCTCTTACAGTTAATGAAAAA5750.1306764723261321No Hit
CTTTAATCCTGAGTGGCAAACTCCCTCCTTTCCTAACATTCATTTACAGG5700.1295401551754701No Hit
ACATTGACCCGTATAAAGAATTTGGAGCTTCTGTGGAGTTACTCTCTTTT5640.12817657459467566No Hit
CCCTTAACTTCATGGGATATGTAATTGGAAGTTGGGGTACTTTACCACAG5630.12794931116454328No Hit
GGCCTCTACCGTCCCCTTCTTCATCTGCCGTTCCGGCCGACCACGGGGCG5620.12772204773441087No Hit
ATCCTAACCTTACCAAATATTTGCCCTTGGATAAAGGCATTAAACCTTAT5610.12749478430427846No Hit
GTTGTCAATATGCCCTGAGCCTGAGGGCTCCACCCCAAAAGACCGCCGTG5480.1245403597125572No Hit
TATTAACATTGACATAGCTGACTACTAATTCCCTGGATGCTGGATCTTCC5450.12385856942216No Hit
GCCTAATCATCTCATGTTCATGTCCTACTGTTCAAGCCTCCAAGCTGTGC5440.1236313059920276No Hit
CTACTGTACCTGTCTTTAATCCTGAGTGGCAAACTCCCTCCTTTCCTAAC5400.12272225227149798No Hit
TAATAAAACCAAGCGTTGGGGCTATTCCCTTAACTTCATGGGATATGTAA5390.12249498884136557No Hit
GTCTTACATAAGAGGACTCTTGGACTCTCAGCAATGTCAACGACCGACCT5350.12158593512083596No Hit
GTCCCGTGCTGGTAGTTGATGTTCCTGGAAGTAGAGGACAAACGGGCAAC5350.12158593512083596No Hit
GGATAATAAGGTTTAATGCCTTTATCCAAGGGCAAATATTTGGTAAGGTT5320.12090414483043876No Hit
ATACCACATCATCCATATAACTGAAAGCCAAACAGTGGGGGAAAGCCCTA5310.12067688140030636No Hit
ATACAGCACTCAGGCAAGCTATTCTGTGTTGGGGTGAGTTGATGAATCTG5280.11999509110990914No Hit
GTTTACTAGTGCCATTTGTTCAGTGGTTCGTAGGGCTTTCCCCCACTGTT5260.11954056424964432No Hit
CCTTATGAGTCCAGGGGATACTAACATTGAGATTCCCGAGATTGAGATCT5210.1184042470989823No Hit
CCCCTATCTTATCAACACTTCCGGAAACTACTGTTGTTAGACGACGAGGC5130.11658613965792308No Hit
CCTCAAGGTCGGTCGTTGACATTGCTGAGAGTCCAAGAGTCCTCTTATGT5060.11499529564699626No Hit
GACGGGGAGACCGCGTAAAGAGAGGTACGCCCCGTGGTCGGCCGGAACGG5040.11454076878673145No Hit
ATTTACATACTCTGTGGAAGGCTGGCATTCTATATAAGAGAGAAACTACA5030.11431350535659904No Hit
GGCTGGGGCTTGGCCATAGGCCATCGGCGCATGCGTGGAACCTTTGTGGC5030.11431350535659904No Hit
GTCTACGTCCCATCGGCGCTGAATCCCGCGGACGACCCGTCTCGGGGCCG4990.11340445163606942No Hit
GGATTAAAGACAGGTACAGTAGAAGAATAAAGCCCAGTAAAGTTTCCCAC4960.11272266134567223No Hit
CTCCCCGTCTGTGCCTTCTCATCTGCCGGACCGTGTGCACTTCGCTTCAC4960.11272266134567223No Hit
GGCTTTCACTTTCTCGCCAACTTACAAGGCCTTTCTGTGTCAACAATATC4890.1111318173347454No Hit
GCTCTGTATCGGGAGGCCTTAGAGTCTCCGGAACATTGTTCACCTCACCA4890.1111318173347454No Hit
CCCCCAACTCCTCCCAGTCCTTAAACAAACAGTCTTTGAAGTATGCCTCA4850.11022276361421578No Hit
GATCCATACTGCGGAACTCCTAGCAGCTTGTTTTGCTCGCAGCCGGTCTG4840.10999550018408338No Hit
GTGTTGGGGTGAGTTGATGAATCTGGCCACCTGGGTGGGAAGTAATTTGG4840.10999550018408338No Hit
GAGTATGTAAATAATGTCTAGTTTTGAAGTAATGATTAACTGCATGTTCA4830.10976823675395096No Hit
GACTTGGCCCCCAATACCACATCATCCATATAACTGAAAGCCAAACAGTG4830.10976823675395096No Hit
CTTGTATTCCCATCCCATCGTCCTGGGCTTTCGCAAGATTCCTATGGGAG4810.10931370989368616No Hit
GCCATAGGCCATCGGCGCATGCGTGGAACCTTTGTGGCTCCTCTGCCGAT4800.10908644646355377No Hit
GTCGTTGACATTGCTGAGAGTCCAAGAGTCCTCTTATGTAAGACCTTGGG4800.10908644646355377No Hit
GCTTTGGGGCATGGACATTGACCCGTATAAAGAATTTGGAGCTTCTGTGG4790.10885918303342136No Hit
ACATAGAGGTTCCTTGAGCAGGAGTCGTGCATGTCTTGCATGGTCCCGTG4770.10840465617315655No Hit
ATTGACAACAGTGCCAGCAGCGCCTCCTCCTGCCTCCACCAATCGGCAGT4770.10840465617315655No Hit
CTCCATGTTCGGTGCAGGGTCCCCAGTCCTCGAGAAGATTGACGATATGG4750.10795012931289175No Hit
GTCCTGGGCTTTCGCAAGATTCCTATGGGAGTGGGCCTCAGTCCGTTTCT4720.10726833902249452No Hit
GGAACATATTGTACTAAAACTCAAGCAATGTTTTCGGAAACTGCCTGTAA4720.10726833902249452No Hit
CCCATAGGAATCTTGCGAAAGCCCAGGACGATGGGATGGGAATACAAGTG4710.10704107559236214No Hit
GTTCAAGCCTCCAAGCTGTGCCTTGGGTGGCTTTGGGGCATGGACATTGA4710.10704107559236214No Hit
GAATACAAGTGCAGTTTCCATCCGAAGGTTTTGTACAGCAACAAGAGGGA4690.10658654873209733No Hit
GTCCTACTGTTCAAGCCTCCAAGCTGTGCCTTGGGTGGCTTTGGGGCATG4680.10635928530196492No Hit
GTTCAAATGTATACCCAAAGACAAAAGAAAATTGGTAATAGAGGTAAAAA4680.10635928530196492No Hit
CCTCCAAGCTGTGCCTTGGGTGGCTTTGGGGCATGGACATTGACCCGTAT4660.10590475844170011No Hit
CTCTAAGGCCTCCCGATACAGAGCAGAGGCGGTGTCGAGGAGATCTCGAA4620.10499570472117051No Hit
GTCCAGGGGATACTAACATTGAGATTCCCGAGATTGAGATCTTCTGCGAC4620.10499570472117051No Hit
GTGGTAAAGTACCCCAACTTCCAATTACATATCCCATGAAGTTAAGGGAA4610.10476844129103809No Hit
GTGCAGAGGTGAAGCGAAGTGCACACGGTCCGGCAGATGAGAAGGCACAG4610.10476844129103809No Hit
TTGACATACTTTCCAATCAATAGGTCTATTTACAGGCAGTTTCCGAAAAC4530.10295033384997887No Hit
TCACCATACAGCACTCAGGCAAGCTATTCTGTGTTGGGGTGAGTTGATGA4520.10272307041984646No Hit
AGTCTGTACAACATCTTGAGTCCCTTTTTACCTCTATTACCAATTTTCTT4520.10272307041984646No Hit
TTTCTGTTCCCAATCCTCTGGGATTCTTTCCCGATCACCAGTTGGACCCT4520.10272307041984646No Hit
TTCCCCCACTGTTTGGCTTTCAGTTATATGGATGATGTGGTATTGGGGGC4510.10249580698971407No Hit
CTATTACCAATTTTCTTTTGTCTTTGGGTATACATTTGAACCCTAATAAA4500.10226854355958165No Hit
GCCTATGGCCAAGCCCCAGCCAGTGGGGGTTGCGTCAGCAAACACTTGGC4490.10204128012944924No Hit
CGTAAAGAGAGGTACGCCCCGTGGTCGGCCGGAACGGCAGATGAAGAAGG4460.10135948983905205No Hit
GCTTAGCTTGTATACATGCATATAAAGGCATCAAAGCAGGATAGCCACAT4440.10090496297878723No Hit
CTTTATATGCATGTATACAAGCCAAGCAGGCTTTCACTTTCTCGCCAACT4430.10067769954865483No Hit
GTACAACATCTTGAGTCCCTTTTTACCTCTATTACCAATTTTCTTTTGTC4410.10022317268839002No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)