FastQCFastQC Report
Sun 4 Sep 2022
EGAF00002490278

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002490278
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences621175
Sequences flagged as poor quality0
Sequence length35-151
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN47410.763230973558176No Hit
ATCCTAACCTTACCAAATATTTGCCCTTGGATAAAGGCATTAAACCGTAT35730.5752002253793215No Hit
GTATGTAAATAATGCCTAGTTTTGAAGTAATGATTAACTGCATTTTCAGG33010.5314122429267115No Hit
CTTATATAGAATGCCAGCCTTCCACAGAGTATGTAAATAATGCCTAGTTT26000.4185615969734777No Hit
CTCTTACAGTTAATGAAAAAAGGAGATTAAAATTAATTATGCCTGCTAGG18920.3045840544130076No Hit
GGTTAGGATAGAACCTAGCAGGCATAATTAATTTTAATCTCCTTTTTTCA16340.2630498651748702No Hit
GTTCTATCCTAACCTTACCAAATATTTGCCCTTGGATAAAGGCATTAAAC15910.25612750030184733No Hit
CTCTTATATAGAATGCCAGCCTTCCACAGAGTATGTAAATAATGCCTAGT15590.25097597295448143No Hit
AGTCTAGACTCTGTGGTATTGTGAGGATTCTTGTCAACAAGAAAAACCCC13910.22393045438081055No Hit
TTATTAATAGATGTCAACAATATGTGGGCCCTCTTACAGTTAATGAAAAA13630.21942286795186544No Hit
AGTCTGTACAACATCTTGAGTCCCTTTTTACCTCTATTACCAATTTTCTT13330.21459331106370988No Hit
TCCACAGAGTATGTAAATAATGCCTAGTTTTGAAGTAATGATTAACTGCA13050.21008572463476474No Hit
TTGTTTACACAGAAAGGCCTTGTAAGTTGGCGAGAAAGTGAAAGCCTGCT12940.20831488710910773No Hit
CTGTAAGAGGGCCCACATATTGTTGACATCTATTAATAATGTCCTCCTGT12230.19688493580713973No Hit
CTATTAATAATGTCCTCCTGTAAATGAATGTGAGGAAAGGAGGGAGTTTG11360.18287922083148872No Hit
GAGTATGTAAATAATGCCTAGTTTTGAAGTAATGATTAACTGCATTTTCA11240.1809473980762265No Hit
GTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATTGCTGAGAGTCCAA11140.17933754578017466No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGGAGCACCCACG11060.1780496639433332No Hit
CCCTTAACTTCATGGGATATGTAATTGGAAGTTGGGGTACTTTACCGCAA10660.17161025475912586No Hit
GTGCTGGTGAACAGACCAATTTATGCCTACAGCCTCCTAGTACAAAGACC10620.17096631384070513No Hit
CTCCAAAAGACACCAAATACTCAAGAACAGTTTCTCTTCCAAAAGTAAGA10590.17048335815188956No Hit
GTATACATGCATATAAAGGCATCAAGGCAGGATAGCCACATTGTGTAAAA9760.15712158409465932No Hit
GTGTACACAATATCTGAACCTTTACCCCGTTGCCCGGCAACGGTCAGGTC9610.15470680565058156No Hit
CATTTGAACCCTAATAAAACCAAACGTTGGGGATACTCCCTTAACTTCAT9280.1493942930736105No Hit
GTCTATTTACAGGCAGTTTTCGAAAACATTGCTTGATTTTTTGTACAATA8900.1432768543486135No Hit
GTACAACATCTTGAGTCCCTTTTTACCTCTATTACCAATTTTCTTTTGTC8850.1424719282005876No Hit
GGATAGAACCTAGCAGGCATAATTAATTTTAATCTCCTTTTTTCATTAAC8660.1394132088380891No Hit
GTTAAGGGAGTATCCCCAACGTTTGGTTTTATTAGGGTTCAAATGTATAC8650.1392522236084839No Hit
GTGTAAAAGGGGCAGCAAAGCCCAGAAGACCCACAATTCTTTGACATACT8460.13619350424598542No Hit
GTTTTGAAGTAATGATTAACTGCATTTTCAGGATAATACGGTTTAATGCC8190.13184690304664548No Hit
ATTGACAACAGTGCCAGCAGCACCTCCTCCTGCCTCCACCAATCGGCAGT8130.13088099166901437No Hit
CCCTAGCAGCCATGGAAAGGAGGTGTATTTCCGAGAGAGGACAACAGAGT8030.12927113937296253No Hit
ATGTGGTATTGGGGGCCAAGTCTGTACAACATCTTGAGTCCCTTTTTACC8030.12927113937296253No Hit
TTGACATACTTTCCAATCAATAGGTCTATTTACAGGCAGTTTTCGAAAAC7980.12846621322493662No Hit
TCTGTGGAGTTACTCTCTTTTTTGCCTTCTGACTTCTTTCCTTCTATTCG7660.12331468587757073No Hit
GTTTAATGCCTTTATCCAAGGGCAAATATTTGGTAAGGTTAGGATAGAAC7640.12299271541836036No Hit
GGATAATACGGTTTAATGCCTTTATCCAAGGGCAAATATTTGGTAAGGTT7630.12283173018875519No Hit
CTATATAAGAGAGAAACTACACGCAGCGCCTCATTTTGTGGGTCACCATA7560.12170483358151889No Hit
GGATAAAGGCATTAAACCGTATTATCCTGAAAATGCAGTTAATCATTACT7400.11912906990783596No Hit
CTTCCACAGAGTATGTAAATAATGCCTAGTTTTGAAGTAATGATTAACTG7380.1188070994486256No Hit
CTACTGTACCTGTCTTTAATCCTGAGTGGCAAACTCCCTCCTTTCCTCAC7270.11703626192296857No Hit
ATTTACAGGCAGTTTTCGAAAACATTGCTTGATTTTTTGTACAATATGTT7160.11526542439731155No Hit
CTTTTACACAATGTGGCTATCCTGCCTTGATGCCTTTATATGCATGTATA7120.11462148347889081No Hit
CAATAGGTCTATTTACAGGCAGTTTTCGAAAACATTGCTTGATTTTTTGT6960.11204571980520787No Hit
CTCATAAGGTGGGAAACTTTACTGGGCTTTATTCTTCTACTGTACCTGTC6930.11156276411639232No Hit
ACCAAGGTATGTTGCCCGTTTGTCCTCTACTTCCAGGAACATCAACTACC6890.1109188231979716No Hit
CCCTTGGACTCATAAGGTGGGAAACTTTACTGGGCTTTATTCTTCTACTG6720.10818207429468345No Hit
ATGTTAATATGGGCCTGAAAATCAGACAACTATTGTGGTTTCACATTTCC6660.10721616291705237No Hit
TTCTTCTACTGTACCTGTCTTTAATCCTGAGTGGCAAACTCCCTCCTTTC6660.10721616291705237No Hit
CATTTACAGGAGGACATTATTAATAGATGTCAACAATATGTGGGCCCTCT6660.10721616291705237No Hit
CTTTAATCCTGAGTGGCAAACTCCCTCCTTTCCTCACATTCATTTACAGG6610.10641123676902645No Hit
GTGTAAACAATATCTGAACCTTTACCCCGTTGCCCGGCAACGGTCAGGTC6580.10592828108021089No Hit
GGTCTATTTACAGGCAGTTTTCGAAAACATTGCTTGATTTTTTGTACAAT6560.10560631062100052No Hit
CTATTACCAATTTTCTTTTGTCTTTGGGTATACATTTGAACCCTAATAAA6540.10528434016179017No Hit
CAGTTATATGGATGATGTGGTATTGGGGGCCAAGTCTGTACAACATCTTG6490.10447941401376423No Hit
ATGTAAATAATGCCTAGTTTTGAAGTAATGATTAACTGCATTTTCAGGAT6470.10415744355455386No Hit
GTTATATGGATGATGTGGTATTGGGGGCCAAGTCTGTACAACATCTTGAG6440.10367448786573832No Hit
ATTTACATACTCTGTGGAAGGCTGGCATTCTATATAAGAGAGAAACTACA6400.10303054694731759No Hit
AGTTAAGGGAGTATCCCCAACGTTTGGTTTTATTAGGGTTCAAATGTATA6310.10158167988087094No Hit
GTTGTCAATATGCCCTGAGCCTGAGGGCTCCACCCCAAAAGACCGCCGTG6220.10013281281442427No Hit
ATATAGAGCTGAGGCGGTGTCGAGGAGATCTCGAATAGAAGGAAAGAAGT6220.10013281281442427No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)