FastQCFastQC Report
Sun 4 Sep 2022
EGAF00002490279

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002490279
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences621175
Sequences flagged as poor quality0
Sequence length35-151
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN47350.7622650621805449No Hit
GTATGTAAATAATGCCTAGTTTTGAAGTAATGATTAACTGCATTTTCAGG38620.6217249567352195No Hit
ATCCTAACCTTACCAAATATTTGCCCTTGGATAAAGGCATTAAACCGTAT33070.5323781543043425No Hit
CTTATATAGAATGCCAGCCTTCCACAGAGTATGTAAATAATGCCTAGTTT25290.40713164567150967No Hit
CTCTTACAGTTAATGAAAAAAGGAGATTAAAATTAATTATGCCTGCTAGG18920.3045840544130076No Hit
CTCTTATATAGAATGCCAGCCTTCCACAGAGTATGTAAATAATGCCTAGT17570.2828510484163078No Hit
GGTTAGGATAGAACCTAGCAGGCATAATTAATTTTAATCTCCTTTTTTCA16870.27158208234394493No Hit
GTTCTATCCTAACCTTACCAAATATTTGCCCTTGGATAAAGGCATTAAAC15520.24984907634724515No Hit
TTATTAATAGATGTCAACAATATGTGGGCCCTCTTACAGTTAATGAAAAA13790.22199863162554834No Hit
AGTCTAGACTCTGTGGTATTGTGAGGATTCTTGTCAACAAGAAAAACCCC13710.22071074978870692No Hit
GAGTATGTAAATAATGCCTAGTTTTGAAGTAATGATTAACTGCATTTTCA12710.2046122268281885No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGGAGCACCCACG12700.2044512415985833No Hit
AGTCTGTACAACATCTTGAGTCCCTTTTTACCTCTATTACCAATTTTCTT12590.20268040407292628No Hit
TCCACAGAGTATGTAAATAATGCCTAGTTTTGAAGTAATGATTAACTGCA12360.19897774379200708No Hit
TTGTTTACACAGAAAGGCCTTGTAAGTTGGCGAGAAAGTGAAAGCCTGCT11630.18722582203082866No Hit
CTGTAAGAGGGCCCACATATTGTTGACATCTATTAATAATGTCCTCCTGT11580.18642089588280275No Hit
CTCCAAAAGACACCAAATACTCAAGAACAGTTTCTCTTCCAAAAGTAAGA11400.18352316174990946No Hit
CCCTTAACTTCATGGGATATGTAATTGGAAGTTGGGGTACTTTACCGCAA10900.17547390026965026No Hit
GTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATTGCTGAGAGTCCAA10860.17482995935122952No Hit
CTATTAATAATGTCCTCCTGTAAATGAATGTGAGGAAAGGAGGGAGTTTG10770.17338109228478288No Hit
GTGCTGGTGAACAGACCAATTTATGCCTACAGCCTCCTAGTACAAAGACC10680.17193222521833623No Hit
GTCTATTTACAGGCAGTTTTCGAAAACATTGCTTGATTTTTTGTACAATA10420.16774660924860144No Hit
GTGTACACAATATCTGAACCTTTACCCCGTTGCCCGGCAACGGTCAGGTC10260.16517084557491848No Hit
GTATACATGCATATAAAGGCATCAAGGCAGGATAGCCACATTGTGTAAAA10080.1622731114420252No Hit
GTGTAAAAGGGGCAGCAAAGCCCAGAAGACCCACAATTCTTTGACATACT9070.14601360325190166No Hit
GTTTTGAAGTAATGATTAACTGCATTTTCAGGATAATACGGTTTAATGCC8980.14456473618545498No Hit
CATTTGAACCCTAATAAAACCAAACGTTGGGGATACTCCCTTAACTTCAT8790.1415060168229565No Hit
GGATAGAACCTAGCAGGCATAATTAATTTTAATCTCCTTTTTTCATTAAC8640.13909123837887874No Hit
GTACAACATCTTGAGTCCCTTTTTACCTCTATTACCAATTTTCTTTTGTC8460.13619350424598542No Hit
ATGTGGTATTGGGGGCCAAGTCTGTACAACATCTTGAGTCCCTTTTTACC8260.13297379965388176No Hit
ATTGACAACAGTGCCAGCAGCACCTCCTCCTGCCTCCACCAATCGGCAGT8030.12927113937296253No Hit
GTTAAGGGAGTATCCCCAACGTTTGGTTTTATTAGGGTTCAAATGTATAC7980.12846621322493662No Hit
TTCTTCTACTGTACCTGTCTTTAATCCTGAGTGGCAAACTCCCTCCTTTC7810.12572946432164847No Hit
TTGACATACTTTCCAATCAATAGGTCTATTTACAGGCAGTTTTCGAAAAC7770.12508552340322773No Hit
GTTTAATGCCTTTATCCAAGGGCAAATATTTGGTAAGGTTAGGATAGAAC7680.1236366563367811No Hit
CTACTGTACCTGTCTTTAATCCTGAGTGGCAAACTCCCTCCTTTCCTCAC7480.12041695174467741No Hit
CTTTTACACAATGTGGCTATCCTGCCTTGATGCCTTTATATGCATGTATA7410.11929005513744113No Hit
ATTTACAGGCAGTTTTCGAAAACATTGCTTGATTTTTTGTACAATATGTT7340.11816315853020486No Hit
TCTGTGGAGTTACTCTCTTTTTTGCCTTCTGACTTCTTTCCTTCTATTCG7340.11816315853020486No Hit
CCCTAGCAGCCATGGAAAGGAGGTGTATTTCCGAGAGAGGACAACAGAGT7260.1168752766933634No Hit
GGATAATACGGTTTAATGCCTTTATCCAAGGGCAAATATTTGGTAAGGTT7230.11639232100454783No Hit
GTGTAGTTTCTCTCTTATATAGAATGCCAGCCTTCCACAGAGTATGTAAA7030.11317261641244417No Hit
CTATATAAGAGAGAAACTACACGCAGCGCCTCATTTTGTGGGTCACCATA7010.1128506459532338No Hit
AGTTAAGGGAGTATCCCCAACGTTTGGTTTTATTAGGGTTCAAATGTATA7000.1126896607236286No Hit
GTGTAAACAATATCTGAACCTTTACCCCGTTGCCCGGCAACGGTCAGGTC6900.11107980842757677No Hit
ATGTAAATAATGCCTAGTTTTGAAGTAATGATTAACTGCATTTTCAGGAT6850.11027488227955086No Hit
CAATAGGTCTATTTACAGGCAGTTTTCGAAAACATTGCTTGATTTTTTGT6850.11027488227955086No Hit
GGTCTATTTACAGGCAGTTTTCGAAAACATTGCTTGATTTTTTGTACAAT6830.10995291182034049No Hit
CCCTTGGACTCATAAGGTGGGAAACTTTACTGGGCTTTATTCTTCTACTG6740.10850404475389382No Hit
ATGTTAATATGGGCCTGAAAATCAGACAACTATTGTGGTTTCACATTTCC6510.1048013844729746No Hit
CTCATAAGGTGGGAAACTTTACTGGGCTTTATTCTTCTACTGTACCTGTC6500.10464039924336943No Hit
CAGTTATATGGATGATGTGGTATTGGGGGCCAAGTCTGTACAACATCTTG6460.10399645832494869No Hit
GGATAAAGGCATTAAACCGTATTATCCTGAAAATGCAGTTAATCATTACT6410.10319153217692276No Hit
CTTCCACAGAGTATGTAAATAATGCCTAGTTTTGAAGTAATGATTAACTG6400.10303054694731759No Hit
CTTTTTACCTCTATTACCAATTTTCTTTTGTCTTTGGGTATACATTTGAA6390.10286956171771239No Hit
ATATGTTCTTGCGGTAAAGTACCCCAACTTCCAATTACATATCCCATGAA6340.10206463556968648No Hit
CATTTACAGGAGGACATTATTAATAGATGTCAACAATATGTGGGCCCTCT6270.1009377389624502No Hit
CTATTACCAATTTTCTTTTGTCTTTGGGTATACATTTGAACCCTAATAAA6260.100776753732845No Hit
CTTTAATCCTGAGTGGCAAACTCCCTCCTTTCCTCACATTCATTTACAGG6240.10045478327363463No Hit
ACCAAGGTATGTTGCCCGTTTGTCCTCTACTTCCAGGAACATCAACTACC6230.10029379804402946No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)