FastQCFastQC Report
Sat 3 Sep 2022
EGAF00002490280

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002490280
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences444872
Sequences flagged as poor quality0
Sequence length35-151
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGCTGGTGAACAGACCAATTTATGCCTACAGCCTCCTAGTACAAAGACC23320.5241957237137874No Hit
GTATACATGCATATAAAGGCATTAAGGCAGGATAGCCACATTGTGTAAAA15380.3457174198421119No Hit
CCTCCCGCTTACAGACCACCAAATGCCCCTATCTTATCAACACTTCCGGA14560.3272851516840799No Hit
GTGTAAAAGGGGCAGCAAAGCCCAAAAGACCCACAATTCTTTGACATACT13450.30233415454332935No Hit
CCCTTAACTTCATGGGATATGTAATTGGAAGTTGGGGTACTTTACCACAG13370.3005358844791311No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN12670.2848010214173965No Hit
TCTGCGGAGTTACTCTCTTTTTTGCCTTCTGACTTCTTTCCTTCTATTCG12390.27850707619270265No Hit
GGGCCAAGTCTGTACAACATCTTGAGTCCCTTTTTACCTCTATTACCAAT11760.2643456994371415No Hit
ATCCTAACCTTACCAAATATTTGCCATTGGATAAAGGCATTAAACCTTAT11330.2546799978420759No Hit
ATATGTTCCTGTGGTAAAGTACCCCAACTTCCAATTACATATCCCATGAA11320.25445521408405114No Hit
AGTCTGTACAACATCTTGAGTCCCTTTTTACCTCTATTACCAATTTTCTT11070.24883562013343166No Hit
ATTCTTTGACATACTTTCCAATCAATAGGTCTATTTACAGGCAGTTTCCG11000.24726213382725817No Hit
CATGTATACAATCTAAGCAGGCTTTTACTTTCTCGCCAACTTACAAGGCC10770.24209210739268824No Hit
GGGTAAAGGTGCAGGTATTGTTGACACAAAAAGGCCTTGTAAGTTGGCGA10460.23512381089392004No Hit
GTATGTAAATAATGCCTAGTTTTGAAGTAATGATTAACTGCATGTTCAGG10430.23444945961984573No Hit
CTCCAAAAGACACCAAATACTCAAGAACAGTTTCTCTTCCAAAAGTAAGA10270.23085291949144923No Hit
GTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATTGCTGAAAGTCCAA10000.22478375802478015No Hit
TCCCGATACAGAGCAGAGGCGGTGTCGAGGAGATCTCGAATAGAAGGAAA9480.2130950026074916No Hit
CATTTGAACCCTAATAAAACCAAACGCTGGGGCTACTCCCTTAACTTCAT9240.20770019241489684No Hit
ACGATGCAGGTCCCCTAGAAGAAGAACTCCCTCGCCTCGCAGACGAAGGT9130.2052275710766243No Hit
TTGACATACTTTCCAATCAATAGGTCTATTTACAGGCAGTTTCCGAAAAC9060.20365408477045086No Hit
GCCATGGGAAGGAGGTGTATTTCCGAGAGAGGACAACAGAGTTGTCAGTC9030.2029797334963765No Hit
GTCTATTTACAGGCAGTTTCCGAAAACATTGCTTGATTTTTAATACAATA9030.2029797334963765No Hit
TCCTAGTACAAAGACCTTTAACCTAATCTCCTCCCCCAACTCCTCCCAGT8830.19848405833588087No Hit
GTGGTAAAGTACCCCAACTTCCAATTACATATCCCATGAAGTTAAGGGAG8540.19196532935316224No Hit
GTATCGGGAGGCCTTAGAGTCTCCGGAACATTGTTCACCTCACCATACAG8490.19084141056303836No Hit
CTCTAAGGCCTCCCGATACAGAGCAGAGGCGGTGTCGAGGAGATCTCGAA8420.1892679242568649No Hit
CTTTAGGGCATGGACATTGACCCGTATAAAGAATTTGGAGCTTCTGCGGA8260.18567138412846843No Hit
GGAACATATTGTATTAAAAATCAAGCAATGTTTTCGGAAACTGCCTGTAA7950.17870308762970022No Hit
GGGCAAAGTCTGTACAACATCTTGAGTCCCTTTTTACCTCTATTACCAAT7810.1755561150173533No Hit
GTACAACATCTTGAGTCCCTTTTTACCTCTATTACCAATTTTCTTTTGTC7720.1735330611951303No Hit
CTGGTGAACAGACCAATTTATGCCTACAGCCTCCTAGTACAAAGACCTTT7580.17038608858278337No Hit
GACTTTGCCCCCAATACCACATCATCCATATAACTGAAAGCCAAACAGTG7420.16678954845438687No Hit
CTCTTACAGTTAATGAAAAAAGGAGATTAAAATTAATTATGCCTGCTAGG7170.16116995450376737No Hit
ATTTACAGGCAGTTTCCGAAAACATTGCTTGATTTTTAATACAATATGTT7150.16072038698771782No Hit
GCTTTAGGGCATGGACATTGACCCGTATAAAGAATTTGGAGCTTCTGCGG6960.15644949558524698No Hit
GGTCTATTTACAGGCAGTTTCCGAAAACATTGCTTGATTTTTAATACAAT6820.15330252297290006No Hit
GATTGTATACATGCATATAAAGGCATTAAGGCAGGATAGCCACATTGTGT6730.15127946915067705No Hit
CCTCAAGGTCGGTCGTTGACATTGCTGAAAGTCCAAGAGTCCTCTTATGT6670.14993076660252835No Hit
CATATAAAGGCATTAAGGCAGGATAGCCACATTGTGTAAAAGGGGCAGCA6650.1494811990864788No Hit
AGTTAAGGGAGTAGCCCCAGCGTTTGGTTTTATTAGGGTTCAAATGTATA6650.1494811990864788No Hit
ATTTATGCCTACAGCCTCCTAGTACAAAGACCTTTAACCTAATCTCCTCC6590.14813249653833013No Hit
CAATAGGTCTATTTACAGGCAGTTTCCGAAAACATTGCTTGATTTTTAAT6470.14543509144203276No Hit
TAGCCACATTGTGTAAAAGGGGCAGCAAAGCCCAAAAGACCCACAATTCT6460.14521030768400797No Hit
TTATTAATAGATGTCAACAATATGTGGGCCCTCTTACAGTTAATGAAAAA6440.14476074016795842No Hit
TTCCCCCACTGTTTGGCTTTCAGTTATATGGATGATGTGGTATTGGGGGC6420.14431117265190885No Hit
GGTTAGGATAGAACCTAGCAGGCATAATTAATTTTAATCTCCTTTTTTCA6390.1436368213778345No Hit
ATGTAATTGGAAGTTGGGGTACTTTACCACAGGAACATATTGTATTAAAA6370.14318725386178496No Hit
ATGTTAATATGGGCCTAAAAATCAGACAACTATTGTGGTTTCACATTTCC6330.14228811882968584No Hit
ATACAGAGCAGAGGCGGTGTCGAGGAGATCTCGAATAGAAGGAAAGAAGT6320.14206333507166105No Hit
CTTTTTACCTCTATTACCAATTTTCTTTTGTCTTTGGGTATACATTTGAA6150.13824201118523982No Hit
ATGTGGTATTGGGGGCAAAGTCTGTACAACATCTTGAGTCCCTTTTTACC6090.13689330863709112No Hit
CTTTTACACAATGTGGCTATCCTGCCTTAATGCCTTTATATGCATGTATA6050.135994173604992No Hit
GTTCAAATGTATACCCAAAGACAAAAGAAAATTGGTAATAGAGGTAAAAA6010.13509503857289287No Hit
TCCCATGGCTGCTCGGATGTGCTGCCAACTGGATCCTGCGCGGGACGTCC5980.13442068729881854No Hit
TCTGTGGAGTTACTCTCTTTTTTGCCTTCTGACTTCTTTCCTTCTATTCG5960.13397111978276896No Hit
CTATTACCAATTTTCTTTTGTCTTTGGGTATACATTTGAACCCTAATAAA5890.1323976334765955No Hit
GCTTAGATTGTATACATGCATATAAAGGCATTAAGGCAGGATAGCCACAT5870.13194806596054595No Hit
CGGTGGTCTCCATGCGACGTGCAGAGGTGAAGCGAAGTGCACACGGTCCG5860.1317232822025212No Hit
GGATAGCCACATTGTGTAAAAGGGGCAGCAAAGCCCAAAAGACCCACAAT5840.13127371468647162No Hit
CTTTACCACAGGAACATATTGTATTAAAAATCAAGCAATGTTTTCGGAAA5660.12722760704202557No Hit
GGGTATGTAAATAATGCCTAGTTTTGAAGTAATGATTAACTGCATGTTCA5640.126778039525976No Hit
CCAAAAGACACCAAATACTCAAGAACAGTTTCTCTTCCAAAAGTAAGACA5630.12655325576795123No Hit
ATACCACATCATCCATATAACTGAAAGCCAAACAGTGGGGGAAAGCCCTG5580.12542933697782735No Hit
GTCGTTGACATTGCTGAAAGTCCAAGAGTCCTCTTATGTAAGAGCTTGGG5580.12542933697782735No Hit
GTATACATTTGAACCCTAATAAAACCAAACGCTGGGGCTACTCCCTTAAC5540.12453020194572821No Hit
GTCCGGCAGATGAGAAGGCACAGACGGGGAGACCGCGTAAAGAGAGGTGC5500.12363106691362909No Hit
CCTTTACCCCGTTGCCCGGCAACGGTCAGGCCTCTGCCAAGTGTTTGCTG5470.12295671563955475No Hit
CTCCCATAGGAATCTTGCGAAAGCCCAGGATGATGGGATGGGAATACAAG5450.12250714812350519No Hit
GTTTTATTAGGGTTCAAATGTATACCCAAAGACAAAAGAAAATTGGTAAT5420.12183279684943085No Hit
CCTTTAACCTAATCTCCTCCCCCAACTCCTCCCAGTCTTTAAACAAACAG5420.12183279684943085No Hit
GTTCTATCCTAACCTTACCAAATATTTGCCATTGGATAAAGGCATTAAAC5400.12138322933338129No Hit
GTTTACTAGTGCCATTTGTTCAGTGGTTCGCAGGGCTTTCCCCCACTGTT5300.11913539175313348No Hit
CTTCAAAGACTGTTTGTTTAAAGACTGGGAGGAGTTGGGGGAGGAGATTA5300.11913539175313348No Hit
GCCCAAAAGACCCACAATTCTTTGACATACTTTCCAATCAATAGGTCTAT5290.1189106079951087No Hit
GTCCAAGAGTCCTCTTATGTAAGAGCTTGGGCAAGACCTGGTGGGCGTTC5200.11688755417288568No Hit
GTACAAAGACCTTTAACCTAATCTCCTCCCCCAACTCCTCCCAGTCTTTA5200.11688755417288568No Hit
CTAGTAAACTGAGCCAGGAGAAACGGACTGAGGCCCACTCCCATAGGAAT5200.11688755417288568No Hit
GCTTTGGGGCATGGACATTGACCCGTATAAAGAATTTGGAGCTTCTGCGG5180.11643798665683612No Hit
CTTTTATATAGAATGCCAGCCTTCCACAGGGTATGTAAATAATGCCTAGT5170.11621320289881135No Hit
CTCAAGAACAGTTTCTCTTCCAAAAGTAAGACAGGAAATGTGAAACCACA5170.11621320289881135No Hit
GTCTTTAAACAAACAGTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACA5160.11598841914078656No Hit
CAATATGTTCCTGTGGTAAAGTACCCCAACTTCCAATTACATATCCCATG5150.11576363538276178No Hit
AGTCTAGACTCTGTGGTATTGTGAGGATTCTTGTCAACAAGAAAAACCCC5130.11531406786671222No Hit
GAGCAGAGGCGGTGTCGAGGAGATCTCGAATAGAAGGAAAGAAGTCAGAA5120.11508928410868743No Hit
ACCTTGAGGCATACTTCAAAGACTGTTTGTTTAAAGACTGGGAGGAGTTG5120.11508928410868743No Hit
GACCAATTTATGCCTACAGCCTCCTAGTACAAAGACCTTTAACCTAATCT5070.11396536531856354No Hit
CAGTTATATGGATGATGTGGTATTGGGGGCAAAGTCTGTACAACATCTTG5060.11374058156053876No Hit
GGGTATACATTTGAACCCTAATAAAACCAAACGCTGGGGCTACTCCCTTA5030.11306623028646441No Hit
GTTATATGGATGATGTGGTATTGGGGGCAAAGTCTGTACAACATCTTGAG5000.11239187901239008No Hit
GCACGACTCCTGCTCAAGGAACCTCTATGTTTCCCTCTTGTTGCTGTACA4980.11194231149634051No Hit
GCCTTAGAGTCTCCGGAACATTGTTCACCTCACCATACAGCACTCAGGCA4970.11171752773831574No Hit
CCTCTATGTTTCCCTCTTGTTGCTGTACAAAACCTTCGGACGGAAACTGC4940.11104317646424142No Hit
GTCTTTGGGTATACATTTGAACCCTAATAAAACCAAACGCTGGGGCTACT4830.10857055512596883No Hit
CCTCTGCACGTCGCATGGAGACCACCGTGAACGCCCACCAGGTCTTGCCC4800.10789620385189447No Hit
GGATATGTAATTGGAAGTTGGGGTACTTTACCACAGGAACATATTGTATT4760.10699706881979537No Hit
CTATTAATAATGTCCTCCTGTAAATGAATGTTAGGAAAGGAGGGAGTTTG4750.10677228506177057No Hit
ATACAATATGTTCCTGTGGTAAAGTACCCCAACTTCCAATTACATATCCC4730.10632271754572101No Hit
GCCTAATCATCTCATGTTCATGTCCTACTGTTCAAGCCTCCAAGCTGTGC4650.10452444748152279No Hit
GTAGAGGCCCAAACGGCCCCGAGACGGGTCGTCCGCGGGATTCAGCGCCG4650.10452444748152279No Hit
GGGTTGAGTTGATGAATCTGGCCACCTGGGTGGGAAGTAATTTGGAAGAC4650.10452444748152279No Hit
GGATAATAAGGTTTAATGCCTTTATCCAATGGCAAATATTTGGTAAGGTT4570.10272617741732452No Hit
TCCACAGGGTATGTAAATAATGCCTAGTTTTGAAGTAATGATTAACTGCA4560.10250139365929975No Hit
CTGTACAACATCTTGAGTCCCTTTTTACCTCTATTACCAATTTTCTTTTG4540.10205182614325019No Hit
GTGCAGAGGTGAAGCGAAGTGCACACGGTCCGGCAGATGAGAAGGCACAG4530.10182704238522543No Hit
GTATGGATCGGCAGAGGAGCCACAAAGGTTCCACGCATGCGCCGATGGCC4530.10182704238522543No Hit
CTTGTATTCCCATCCCATCATCCTGGGCTTTCGCAAGATTCCTATGGGAG4520.10160225862720063No Hit
CTGTAAGAGGGCCCACATATTGTTGACATCTATTAATAATGTCCTCCTGT4470.10047833983707674No Hit
GTATAAAGAATTTGGAGCTTCTGCGGAGTTACTCTCTTTTTTGCCTTCTG4470.10047833983707674No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)