FastQCFastQC Report
Fri 2 Sep 2022
EGAF00002490284

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002490284
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences379587
Sequences flagged as poor quality0
Sequence length35-151
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGCTGGTGCGCAGACCAATTTATGCCTACAGCCTCCTAGTACAAAGATC20540.5411144217267715No Hit
TCCATAGAGTGTGTAAATAATGTCTGGTTTGGAAGTAATGATTAACCAGA15320.4035965404505423No Hit
CCTCCAGCTTATAGACCACCAAATGCCCCTATCTTATCAACACTTCCGGA14550.38331133574121345No Hit
CTCTATGGAAGGCGGGTATATTATATAAGAGAGAAACAACACATAGCGCC12710.3348375998124277No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGGAACTACCGTG11930.31428895088609465No Hit
ATGTAAGACCTTGGGCAAGAATTGGTGGGCGTTCACGGTGGTCTCCATGC10540.27767020472250104No Hit
GTGTAAAAGGGGCAGCAAAACCCAAAAGACCCACAATACGTTGACAGACT10530.2774067605054968No Hit
AGTCTAGACTCTGCGGTATTGTGAGGATTCTTGTCAACAAGAAAAACCCC10500.2766164278544839No Hit
GGCTTTCACTTTCTCGCCAACTTACAAGGCCTTTCTGTGTAAACAATACC10380.2734550972504327No Hit
GTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATTACAGAGAGTCCAA9580.2523795598900911No Hit
GTACAGACTTGGCCCCCAATACCACATCATCCATATAACTGAAAGCCAAA8760.2307771340957409No Hit
CCATACTCTTGGGAACAAGAGCTACAGCATGGGGCAGAATCTTTCCACCA8310.2189221443305487No Hit
CTATGTATCCCTCCTGTTGCTGTACCAAACCTTCGGACGGAAATTGCACC8140.21444359264147614No Hit
GTCCAAGAGTCCTCTTATGTAAGACCTTGGGCAAGAATTGGTGGGCGTTC8030.21154570625442917No Hit
CATTTACACCAAGACATTATCAAAAAATGTGAACAATTTGTAGGCCCACT7380.1944218321491516No Hit
CCTCAAGCTTATAGACCACCAAATGCCCCTATCTTATCAACACTTCCGGA7370.1941583879321473No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN7340.1933680552811345No Hit
CCTACAGCTTATAGACCACCAAATGCCCCTATCTTATCAACACTTCCGGA7320.19284116684712596No Hit
ATGTCATAGGATGCTATGGGTCATTGCCACAAGATCACATCATACAGAAA7020.18493784033699784No Hit
CATTTAAACCCTAACAAAACAAAAAGGTGGGGTTACTCTTTACATTTCAT6970.18362061925197648No Hit
GCCTAATCATCTCTTGTTCATGTCCTACTGTTCAAGCCTCCAAGCTGTGC6930.18256684238395943No Hit
CCTCCAGCTTATAGACCACAAAATGCCCCTATCTTATCAACACTTCCGGA6810.17940551177990816No Hit
CTACAAACCTTGCCAACAAATCCGCCTCCTGCCTCTACCAATCGCCAGTC6760.17808829069488682No Hit
GGATAACCACATTGTGTAAAAGGGGCAGCAAAACCCAAAAGACCCACAAT6680.17598073695885263No Hit
GTATGGATCGGCAGAGGAGCCAGAAAGGTTCCACGCATGCGCTGATGGCC6630.1746635158738313No Hit
GGAACATACTGCACTCAGGCAAGCAATTCTTTGCTGGGGGAACTAATGAC6600.1738731832228185No Hit
CCCTTATCCAATGGCAAATATTTGGTAACATTTGGATAAAACCTAGCAGG6540.17229251792079286No Hit
GTGTAAACAATACCTGAACCTTTACCCCGTTGCCCGGCAACGGCCAGGTC6480.17071185261876723No Hit
TCCTCAGGCCATGCAGTGGAACTCCACAACCTTCCACCAAACTCTGCAAG6450.16992151996775443No Hit
GGGTAAAGGTTCAGGTATTGTTTACACAGAAAGGCCTTGTAAGTTGGCGA6430.16939463153374587No Hit
CCATATTCTTGGGAACAAGAGCTACAGCATGGGGCAGAATCTTTCCACCA6420.16913118731674162No Hit
ACATAGAGGTTCCTTGAGCAGGAGTCGTGCAGGTTCTGCATGGTCCCGTG6420.16913118731674162No Hit
GCACGGGACCATGCAGAACCTGCACGACTCCTGCTCAAGGAACCTCTATG6410.16886774309973734No Hit
AGCTTGAGGAGTGCGAATCCACACTCCGAAAGACACCAAATACTCTATGA6330.16676018936370318No Hit
GCCTAGCAGCCATGGAAACGATGTATATTTGCGGGAGAGAACAACAGAGT6310.16623330092969466No Hit
AATATGGGCCTAAAGTTCAGGCAACTATTGTGGTTTCACATTTCTTGTCT6300.16596985671269035No Hit
GCGCTGAACATGGAGAACATCACATCAGGATTCCTAGGACCCCTGCTCGT6210.16359885875965194No Hit
CTTTTACACAATGTGGTTATCCTGCTTTAATGCCTTTGTATGCATGTATT6200.16333541454264766No Hit
GTGTAAATGTATATTAGGAAAAGAGGGTGTTTTCCAATGAGGGTTAAAGA6080.16017408393859645No Hit
CCACCAAATGCCCCTATCTTATCAACACTTCCGGAGACTACTGTTGTTAG6060.1596471955045879No Hit
ACTGTGGAGTTACTCTCGTTTTTGCCTTCTGACTTCTTTCCTTCCGTACG6030.15885686285357506No Hit
ACATTACAGAGAGTCCAAGAGTCCTCTTATGTAAGACCTTGGGCAAGAAT6000.15806653020256226No Hit
CTAGTAAACTGAGCCAGGAGAAACGGGCTGAGGCCCACTCCCATAGGAAT5960.15701275333454517No Hit
GCTGTAGGAGTGCGAATCCACACTCCGAAAGACACCAAATACTCTATGAC5940.15648586490053662No Hit
GTAGAAGAGTAAAGCCCCGTAAAGTTTCCCACCTTATGAGTCCAAGGAAT5870.15464175538150673No Hit
TCCTATGACATAGCCCATGAAATGTAAAGAGTAACCCCACCTTTTTGTTT5850.1541148669474982No Hit
AGCTGGAGGAGTGCGAATCCACACTCCGAAAGACACCAAATACTCTATGA5850.1541148669474982No Hit
ATATAAGAGAGAAACAACACATAGCGCCTCATTCTGTGGGTCACCATATT5760.15174386899445977No Hit
AGTCTGTACAGCATCTTGAGTCCCTTTTTACCGCTGTTACCAATTTTCTT5620.14805564995639997No Hit
GGTTTGTAGTATGCCCTGAGCCTGAGGGCTCCACCCCAAAAGGCCTCCGT5600.14752876152239144No Hit
CTCTATGACCGTTTCTCTTCCAAAAGTGAGACAAGAAATGTGAAACCACA5500.14489431935234875No Hit
GTATGGTGAGGTGAACAATGCTCAGGAGACTCTAAGGCTTCCCGATACAG5410.14252332139931032No Hit
GACGGGGAGTCCGCGTAAAGAGAGGTGCGCCCCGTGGTCGGACGAAACGG5390.14199643296530176No Hit
GGGTTTACTGTTCCTGAACTGGAGCCACCAGCAGGGAAATACAGGCCTCT5320.14015232344627188No Hit
CCTCTATGTATCCCTCCTGTTGCTGTACCAAACCTTCGGACGGAAATTGC5300.13962543501226332No Hit
CTATGACATAGCCCATGAAATGTAAAGAGTAACCCCACCTTTTTGTTTTG5290.13936199079525904No Hit
TTATATAAGAGAGAAACAACACATAGCGCCTCATTCTGTGGGTCACCATA5280.1390985465782548No Hit
CTCTTATATAATATACCCGCCTTCCATAGAGTGTGTAAATAATGTCTGGT5230.13778132549323344No Hit
TACCTTGATAGTCCAGAAGAACCAACAAGAAGATGAGGCATAGCAGCAGG5230.13778132549323344No Hit
ATTTTGTGGTCTATAAGCTGGAGGAGTGCGAATCCACACTCCGAAAGACA5190.13672754862521636No Hit
CCTTTACCCCGTTGCCCGGCAACGGCCAGGTCTGTGCCAAGTGTTTGCTG5170.1362006601912078No Hit
ACGATGCAGGTCCCCTAGAAGAAGAACTCCCTCGCCTCGCAGACGAAGGT4980.13119522006812667No Hit
GTTCCGCGCAAGGTCCCCAATCCTCGAGAAGATTGACGATATGTGAGAGA4940.1301414432001096No Hit
GTTTAAATGTATACCCAAAGACAAAAGAAAATTGGTAACAGCGGTAAAAA4940.1301414432001096No Hit
CCTGTATTCCCATCCCATCATCCTGGGCTTTCGGAAAATTCCTATGGGAG4930.12987799898310531No Hit
GCCTGAGGATGAGTGTTTCTCAAAGGTGGAGACAGAGGGGTAGGCTGCCT4910.1293511105490968No Hit
GTCCCGTGCTGGTGGTTGAAGATCCTGGAATTAGAGGACAAACGGGCAAC4910.1293511105490968No Hit
GTACCAAACCTTCGGACGGAAATTGCACCTGTATTCCCATCCCATCATCC4890.12882422211508823No Hit
GTATTTCCCTGCTGGTGGCTCCAGTTCAGGAACAGTAAACCCTGTTCCGA4870.1282973336810797No Hit
ATTTATGCCTACAGCCTCCTAGTACAAAGATCATTAATCTAATCTCCTCC4830.12724355681306262No Hit
CAGTAAACCCTGTTCCGACTACTGTCTCTCACATATCGTCAATCTTCTCG4760.12539944729403274No Hit
GTATGCATGTATTCAATCTAAGCAGGCTTTCACTTTCTCGCCAACTTACA4740.12487255886002419No Hit
ATACCACATCATCCATATAACTGAAAGCCAAACAGTGGGGGAAAGCCCTA4710.12408222620901137No Hit
GGAAGCCTTAGAGTCTCCTGAGCATTGTTCACCTCACCATACTGCACTCA4700.1238187819920071No Hit
GGGGACCTAATGACTCTAGCCACCTGGGTGGGTGGTAATTTGGAAGATCC4700.1238187819920071No Hit
ATGTATATTTGCGGGAGAGAACAACAGAGTTATCCGTCCCGATAATGTTT4680.12329189355799856No Hit
CGGTGGTCTCCATGCGACGTGCAGAGGTGAAGCGAAGTGCACACGGCCCG4650.12250156090698576No Hit
GTGCAGAGGTGAAGCGAAGTGCACACGGCCCGGCAGATGAGAAGGCACAG4640.12223811668998147No Hit
GGAATTAGAGGACAAACGGGCAACATACCTTGATAGTCCAGAAGAACCAA4630.12197467247297722No Hit
GATACATAGAGGTTCCTTGAGCAGGAGTCGTGCAGGTTCTGCATGGTCCC4630.12197467247297722No Hit
CTTCTACTGTTCCTGTCTTTAACCCTCATTGGAAAACACCCTCTTTTCCT4610.12144778403896866No Hit
GTCATAGGATGCTATGGGTCATTGCCACAAGATCACATCATACAGAAAAT4610.12144778403896866No Hit
GTCCAGAAGAACCAACAAGAAGATGAGGCATAGCAGCAGGATGAAGAGGA4500.1185498976519217No Hit
GGATAAGGGTATCAAACCTTATTATCCAGAACATCTGGTTAATCATTACT4470.1177595650009089No Hit
GAGTTATCCGTCCCGATAATGTTTGCTCCAGACCTGCTGCGAGCAAAACA4430.1167057881328918No Hit
CTGTAATGTCAACGACCGACCTTGAGGCATACTTCAAAGACTGTTTGTTT4430.1167057881328918No Hit
ATGTGGTATTGGGGGCCAAGTCTGTACAGCATCTTGAGTCCCTTTTTACC4410.11617889969888326No Hit
GTTTACTAGTGCCATTTGTTCAGTGGTTCGTAGGGCTTTCCCCCACTGTT4410.11617889969888326No Hit
GCTTTAATGCCTTTGTATGCATGTATTCAATCTAAGCAGGCTTTCACTTT4370.11512512283086618No Hit
ATCAAGGTATGTTGCCCGTTTGTCCTCTAATTCCAGGATCTTCAACCACC4360.11486167861386191No Hit
GGCCTATTGATTGGAAAGTCTGTCAACGTATTGTGGGTCTTTTGGGTTTT4350.11459823439685764No Hit
GAACAAATGGCACTAGTAAACTGAGCCAGGAGAAACGGGCTGAGGCCCAC4330.11407134596284908No Hit
TAACCACATTGTGTAAAAGGGGCAGCAAAACCCAAAAGACCCACAATACG4320.11380790174584483No Hit
CAGTTATATGGATGATGTGGTATTGGGGGCCAAGTCTGTACAGCATCTTG4250.11196379222681493No Hit
TGTTTACACAGAAAGGCCTTGTAAGTTGGCGAGAAAGTGAAAGCCTGCTT4250.11196379222681493No Hit
ATCCCAGAGTGAGAGGCCTGTATTTCCCTGCTGGTGGCTCCAGTTCAGGA4250.11196379222681493No Hit
GAATACATGCATACAAAGGCATTAAAGCAGGATAACCACATTGTGTAAAA4240.11170034800981066No Hit
CTCTGGGATCTTGCAGAGTTTGGTGGAAGGTTGTGGAGTTCCACTGCATG4220.11117345957580212No Hit
CTTATATAATATACCCGCCTTCCATAGAGTGTGTAAATAATGTCTGGTTT4170.10985623849078076No Hit
CACCAAGACATTATCAAAAAATGTGAACAATTTGTAGGCCCACTTACAGT4150.10932935005677222No Hit
GAATACAGGTGCAATTTCCGTCCGAAGGTTTGGTACAGCAACAGGAGGGA4100.10801212897175087No Hit
GAATACTAACATTGAGATTCCCGAGATTGAGATCTTCTGCGACGCGGCGA4090.10774868475474661No Hit
GTCTTTAAACAAACAGTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACA4080.10748524053774233No Hit
CATGCATACAAAGGCATTAAAGCAGGATAACCACATTGTGTAAAAGGGGC4050.10669490788672953No Hit
ATATAACTGAAAGCCAAACAGTGGGGGAAAGCCCTACGAACCACTGAACA4040.10643146366972525No Hit
AGAATATGGTGACCCACAGAATGAGGCGCTATGTGTTGTTTCTCTCTTAT4030.10616801945272099No Hit
GTTCATGTCCTACTGTTCAAGCCTCCAAGCTGTGCCTTGGGTGGCTTTGG3980.10485079836769964No Hit
GTCCAAGGAATACTAACATTGAGATTCCCGAGATTGAGATCTTCTGCGAC3940.10379702149968255No Hit
CTGCTAGGCTGTGCTGCCAACTGGATCCTGCGCGGGACGTCCTTTGTTTA3940.10379702149968255No Hit
AATATACATCGTTTCCATGGCTGCTAGGCTGTGCTGCCAACTGGATCCTG3900.10274324463166547No Hit
CCAGATGTTCTGGATAATAAGGTTTGATACCCTTATCCAATGGCAAATAT3890.1024798004146612No Hit
CCTTATGAGTCCAAGGAATACTAACATTGAGATTCCCGAGATTGAGATCT3860.10168946776364837No Hit
ATGTGGTTATCCTGCTTTAATGCCTTTGTATGCATGTATTCAATCTAAGC3850.10142602354664412No Hit
GAATCTTTCCACCAGCAATCCTCTGGGATTCTTTCCCGACCACCAGTTGG3820.10063569089563132No Hit
GAGTAACCCCACCTTTTTGTTTTGTTAGGGTTTAAATGTATACCCAAAGA3820.10063569089563132No Hit
CAATAGGCCTGTTAACAGGAAGTTTTCTAAAACATTCTTTGATTTTCTGT3810.10037224667862703No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)