FastQCFastQC Report
Fri 2 Sep 2022
EGAF00002490289

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002490289
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences359327
Sequences flagged as poor quality0
Sequence length35-151
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGCTGGTGCGCAGACCAATTTATGCCTACAGCCTCCTAGTACAAAGACC20870.5808080105307979No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN15600.43414494318545505No Hit
GTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACAGTACAGAGAGTCCAA11960.3328444564421822No Hit
GTCCTACTGTTCAAGCCTCCAAGCTGTGCCTTGGGTGGCTTTAGGACATG8910.24796355408861567No Hit
TCCATAGAGTGTGTAAATAATGTCTGGTTTGGAAGTAATGATTAACTAAA7760.21595927943071352No Hit
CCTCAAGCTTATAGACCACCAAATGCCCCTATCTTATCCACACTTCCGGA7750.21568098139021002No Hit
ATCATCTTCCTCTTCATCCTGCTGCTATGCCTCATCTTCTTGTTGGTTCT6970.19397373423093728No Hit
CCTTTAATCTAAGCTCCTCCCCCAACTCCTCCCAGTCTTTAAACAAACAG6820.1897992636233848No Hit
GCCCTGGAGTCTCCTGATCATTGTACACCTCACCATACTGCGCTCAGGCA6710.18673798517784637No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGGAACCACCGTG6610.1839550047728114No Hit
CTCTAATTCCAGGATCTTCAACCACCAGCGCGGGACCATGCAGAACATGC6570.1828418126107974No Hit
TATCCACACTTCCGGAAACTACTGTTGTTAGACGACGAGGCAGGTCCCCT6490.18061542828676944No Hit
GTACAGAGAGTCCAAGAGTCCTCTTATGTAAGACCTTTGGCAAGAATTGG6390.17783244788173447No Hit
GTCCAAGAGTCCTCTTATGTAAGACCTTTGGCAAGAATTGGTGGGCGTTC6080.16920520862612606No Hit
GTGTAAAAGGGGCAGCAAACCCCAAAAGACCCACAATACGTTGACAGACT6080.16920520862612606No Hit
AGTCTAGACTCTGCGGTATTGTGAGGATTCTTGTCAACAAGAAAAACCCC5980.1664222282210911No Hit
GAATCAGAGGACATACGGGCAACATACCCTGATAGTCCAGAAGAACCAAC5830.16224775761353863No Hit
CTCTTATATAATACACCCGCCTTCCATAGAGTGTGTAAATAATGTCTGGT5740.15974307524900716No Hit
CCCTCGATGTTGGATCTTGCAAATTAGTACCCACCCAGGTAGCTAGATTC5730.1594647772085037No Hit
GTTCAGTGCAGGGTCCCCAATCCTCGATAAGATTGACGATATGGGAGAGG5520.15362051835793025No Hit
GTCATAGAGTATTTGGTGTCTTTCGGAGTGTGGATTCGCACTCCTCAAGC5360.1491677497098743No Hit
CTCTATGACTGTTTCTCTTCCAAAAGTGAGACAAGAAAGGTGAAACCACA5310.1477762595073568No Hit
CTCCAAGGCATCCCGATACAGAGCTGAGACGGTATCTAGAAGATCTCGTA5290.14721966342634982No Hit
ATTTATGCCTACAGCCTCCTAGTACAAAGACCTTTAATCTAAGCTCCTCC5110.14221029869728685No Hit
TCACCATACTGCGCTCAGGCAAGCCGTACTTTGCTGGGGTGACCTAATGA4970.1383141261302379No Hit
GCCTTGGAGTCTCCTGATCATTGTACACCTCACCATACTGCGCTCAGGCA4950.13775753004923091No Hit
TCCTAGTACAAAGACCTTTAATCTAAGCTCCTCCCCCAACTCCTCCCAGT4900.13636603984671344No Hit
ATATAAGAGAGAGACAACACGTAGCGCCTCATTTTGTGGGTCACCATATT4880.13580944376570644No Hit
TCATCTTCCTCTTCATCCTGCTGCTATGCCTCATCTTCTTGTTGGTTCTT4870.13553114572520295No Hit
GTCACTTATGTCAACACTACTGTGGGCCTAAAGTTCAGGCAACTATTGTG4860.13525284768469947No Hit
CATTTAAACCCTAACAAAACTAAAAGATGGGGTTACTCTTTAAATTTCAT4850.13497454964419597No Hit
CTATAAGCTTGAGGAGTGCGAATCCACACTCCGAAAGACACCAAATACTC4650.129408588834126No Hit
GTCTTTAAACAAACAGTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACA4620.12857369471261554No Hit
GGAATATGATAAAACGCCGCAGACACATCCAACGATAACCAGGACAAGTT4540.12634731038858757No Hit
CTTTAGGACATGGACATTGACCCTTATAAAGAATTTGGAGCTTCTGTGGA4500.12523411822657357No Hit
CATCTAGGGACCTAGTAGTCAGGTATGTTAACGAGACTGTGGGCCTAAAG4490.12495582018607007No Hit
GACCAATTTATGCCTACAGCCTCCTAGTACAAAGACCTTTAATCTAAGCT4410.1227294358620421No Hit
CTTTAGGACATGGACATTGACCCTTATAAAGAATTTGGAGCTTCTGTCGA4400.1224511378215386No Hit
GTGTCGATAAGATAGGGGCATTTGGTGGTCTATAAGCTTGAGGAGTGCGA4390.12217283978103512No Hit
GTACAAAGACCTTTAATCTAAGCTCCTCCCCCAACTCCTCCCAGTCTTTA4370.1216162437000281No Hit
ACACTACTGTGGGCCTAAAGTTCAGGCAACTATTGTGGTTTCACCTTTCT4110.11438049464693718No Hit
ATGTAAGACCTTTGGCAAGAATTGGTGGGCGTTCACGGTGGTCTCCATTC4080.1135456005254267No Hit
GCCTAATCATCTCTTGTTCATGTCCTACTGTTCAAGCCTCCAAGCTGTGC3970.11048432207988823No Hit
TTATATGCCTGTATTCAATCTAAGCAGGCTTTCACTTTCTCGCCAACTTA3930.10937112991787426No Hit
CTCTATGGAAGGCGGGTGTATTATATAAGAGAGAGACAACACGTAGCGCC3890.10825793775586026No Hit
GGGTAAAGGTTCAGGTATTGTTTACACAGAAAGGCCTTGTAAGTTGGCGA3890.10825793775586026No Hit
TCCAACGATAACCAGGACAAGTTGGAGGACAGGAGGTTGGTGAGTGATTG3820.10630985147233579No Hit
CCCCCAACTCCTCCCAGTCTTTAAACAAACAGTCTTTGAAGTATGCCTCA3810.10603155343183229No Hit
CTTTTACACAATGTGGTTATCCTGCTTTAATGCCTTTATATGCCTGTATT3740.10408346714830781No Hit
CCTCAAGCTTATAGAGAAGACGTCGAATGGAGACCACCGTGAACGCCCAC3740.10408346714830781No Hit
TTGTACACCTCACCATACTGCGCTCAGGCAAGCCGTACTTTGCTGGGGTG3740.10408346714830781No Hit
GTCGTTGACAGTACAGAGAGTCCAAGAGTCCTCTTATGTAAGACCTTTGG3650.10157878478377633No Hit
GCCTAGTCATCTCTTGTTCATGTCCTACTGTTCAAGCCTCCAAGCTGTGC3600.10018729458125886No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)