FastQCFastQC Report
Fri 2 Sep 2022
EGAF00002490293

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002490293
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences387128
Sequences flagged as poor quality0
Sequence length35-151
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGCTGGTGCGCAGACCAATTTATGCCTACAGCCTCCGAGTACAAAGACC20820.5378066169329008No Hit
CCTCCAGCTTATAGACCACCAAATGCCCCTATCTTATCAACACTTCCGGA16880.4360314934595276No Hit
TCCATAGAGTGTGTAAATAATGTCTGGTTTGGAAGTAATGATTAACTAGA16860.43551486846727694No Hit
CTCTATGGAAGGCGGGTATATTATATAAGAGAGAAACAACACATAGCGCC15970.4125250563121242No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGGAACTACCGTG13300.3435556198466657No Hit
GTGTAAAAGGGGCAGCAAAACCCAAAAGACCCACAATACGTTGACAAACT13090.3381310574280341No Hit
AGTCTAGACTCTGCGGTATTGTGAGGATTCTTGTCAACAAGAAAAACCCC11340.29292637060610444No Hit
CTACAAACCTTGCCAACAAATCCGCCTCCTGCCTCTACCAATCGCCAGTC10630.2745861833812072No Hit
GTGAAAGGCCTGTATTTCCCTGCTGGTGGCTCCAGTTCCGGAACAGTAAA9940.2567626211485607No Hit
GTACAGACTTGGCCCCCAATACCACATCATCCATATAACTGAAAGCCAAA9380.24229712136554318No Hit
ATATAAGAGAGAAACAACACATAGCGCCTCATTCTGTGGGTCACCATATT9160.2366142464507863No Hit
GGTTTGTAGTATGCCCTGAGCCTGAGGGCTCCACCCCAAAAGGCCTCCGT9000.2324812465127813No Hit
ATGTAAGACCTTGGGCAAGAATTGGTGGGCGTTCACGGTGGTCTCCATGC8530.2203405591948916No Hit
GTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATTACAGAGAGTCCAA8120.2097497468537538No Hit
TACCTTGATAGTCCAGAAGAACCAACAAGAAGATGAGGCATAGCAGCAGG8050.20794155938087663No Hit
CATTTAAACCCTAACAAAACAAAAAGGTGGGGTTACTCTTTACATTTCAT8000.20664999690025004No Hit
CTGTAATGTCAACGACCGACCTTGAGGCATACTTCAAAGACTGTTTGTTT7990.20639168440412473No Hit
GGATAACCACATTGTGTAAAAGGGGCAGCAAAACCCAAAAGACCCACAAT7780.20096712198549319No Hit
CATTTACACCAAGACATTATCAAAAAATGTGAACAATTTGTAGGCCCACT7760.20045049699324255No Hit
GTCCCGTGCTGGTGGTTGAAGATCCTGGAATCAGAGGACAAACGGGCAAC7720.19941724700874128No Hit
GTCCCTTGCTGGTGGTTGAAGATCCTGGAATCAGAGGACAAACGGGCAAC7710.19915893451261596No Hit
GGCTTTCACTTTCTCGCCAACTTACAAGGCCTTTCTGTGTAAACAATACC7360.19011799714823005No Hit
GGCCCCTCACTCTGGGATCTTGCAGAGTTTGGTGGAAGGTTGTGGAGTTC7360.19011799714823005No Hit
GGCCTTTCACTCTGGGATCTTGCAGAGTTTGGTGGAAGGTTGTGGAGTTC7290.18830980967535285No Hit
CCCTTATCCAATGGCAAATATTTGGTAACATTTGGATAAAACCTAGCAGG7200.18598499721022504No Hit
GTGAGGGGCCTGTATTTCCCTGCTGGTGGCTCCAGTTCCGGAACAGTAAA7130.18417680973734785No Hit
TTATATAAGAGAGAAACAACACATAGCGCCTCATTCTGTGGGTCACCATA7130.18417680973734785No Hit
CTATGTATCCCTCCTGTTGCTGTACCAAACCTTCGGACGGAAATTGCACC7120.18391849724122253No Hit
GTGTAAACAATACCTGAACCTTTACCCCGTTGCCCGGCAACGGCCAGGTC6910.178493934822591No Hit
GTGTAAATGTATATTAGGAAAAGAGGGTGTTTTCCAATGAGGGTTAAAGA6770.1748775598768366No Hit
GAACAAATGGCACTAGTAAACTGAGCCAGGAGAAACGGGCTGAGGCCCAC6690.1728110599078341No Hit
ATATTAGTGTTAACATAACTGACTACTAGGTCCCTGGATGTTGGATCTTG6690.1728110599078341No Hit
GTCCAAGAGTCCTCTTATGTAAGACCTTGGGCAAGAATTGGTGGGCGTTC6580.16996962245045566No Hit
GCCTAATCATCTCTTGTTCATGTCCTACTGTTCAAGCCTCCAAGCTGTGC6550.16919468496207973No Hit
CTTTTACACAATGTGGTTATCCTGCTTTAATGCCTTTGTATGCATGTATT6520.16841974747370378No Hit
CACTAATATGGGCCTAAAGTTCAGACAACTATTGTGGTTTCACATTTCTT6430.16609493500857597No Hit
GCAAGGGACCATGCAGAAACTGCACGACTCCTGCTCAAGGAACCTCTATG6430.16609493500857597No Hit
GTAGAAGAGTAAAGCCCCGTAAAGTTTCCCACCTTATGAGTCCAAGGAAT6410.16557831001632534No Hit
GTCATAGAGTATTTGGTGTCTTTCGGAGTGTGGATTCGCACTCCTCCAGC6400.16531999752020005No Hit
TCCTCAGGCCATGCAGTGGAACTCCACAACCTTCCACCAAACTCTGCAAG6370.1645450600318241No Hit
CTCTATGACCGTTTCTCTTCCAAAAGTGAGACAAGAAATGTGAAACCACA6350.16402843503957346No Hit
GCCTTAGAGTCTCCTGAGCATTGTTCGCCTCACCATACTGCACTCAGGCA6300.1627368725589469No Hit
GCCTAGCAGCCATGGAAACGATGTATATTTGCGGGAGAGAACAACAGAGT6140.15860387262094192No Hit
GGGTAAAGGTTCAGGTATTGTTTACACAGAAAGGCCTTGTAAGTTGGCGA6050.1562790601558141No Hit
ATACCACATCATCCATATAACTGAAAGCCAAACAGTGGGGGAAAGCCCTA6010.15524581017131284No Hit
TCTGTGGAGTTACTCTCGTTTTTGCCTTCTGACTTCTTTCCTTCCGTACG5980.1544708726829369No Hit
CTCTTATATAATATACCCGCCTTCCATAGAGTGTGTAAATAATGTCTGGT5950.15369593519456096No Hit
GTTTAAATGTATACCCAAAGACAAAAGAAAATTGGTAACAGCGGTAAAAA5840.15085449773718254No Hit
ATCAAGGTATGTTGCCCGTTTGTCCTCTGATTCCAGGATCTTCAACCACC5830.15059618524105722No Hit
CTAGTAAACTGAGCCAGGAGAAACGGGCTGAGGCCCACTCCCATAGGAAT5790.14956293525655598No Hit
GTCCAGAAGAACCAACAAGAAGATGAGGCATAGCAGCAGGATGAAGAGGA5560.14362174784567378No Hit
CCTTTACCCCGTTGCCCGGCAACGGCCAGGTCTGTGCCAAGTGTTTGCTG5530.14284681035729785No Hit
GCTTTAGGGCATGGACATTGATCCTTATAAAGAATTTGGAGCTTCTGTGG5510.1423301853650472No Hit
GTATGGATCGGCAGAGGAGCCAGAAAGGTTCCACGCATGCGCTGATGGCC5410.13974706040379412No Hit
GTATCGGGATGCCTTAGAGTCTCCTGAGCATTGTTCGCCTCACCATACTG5310.13716393544254096No Hit
GTATGCATGTATTCAATCTAAGCAGGCTTTCACTTTCTCGCCAACTTACA5310.13716393544254096No Hit
GTTTAATACCCTTATCCAATGGCAAATATTTGGTAACATTTGGATAAAAC5290.13664731045029035No Hit
CAATAGGCCTGTTAACAGGAAGTTTTCTAAAACATTCTTTGATTTTCTGT5230.13509743547353847No Hit
CTGCTAGGCTGTGCTGCCAACTGGATCCTGCGCGGGACGTCCTTTGTTTA5220.13483912297741316No Hit
GAATCTTTCCACCAGCAATCCTCTGGGATTCTTTCCCGACCACCAGTTGG5160.13328924800066128No Hit
ACATTACAGAGAGTCCAAGAGTCCTCTTATGTAAGACCTTGGGCAAGAAT5130.13251431051228532No Hit
AGTCTGTACAGCATCTTGAGTCCCTTTTTACCGCTGTTACCAATTTTCTT5100.1317393730239094No Hit
GGATAAGGGTATTAAACCTTATTATCCAGAACATCTAGTTAATCATTACT5080.1312227480316588No Hit
AGCTGGAGGAGTGCGAATCCACACTCCGAAAGACACCAAATACTCTATGA5050.13044781054328283No Hit
GTATTTCCCTGCTGGTGGCTCCAGTTCCGGAACAGTAAACCCTGTTCCGA5030.12993118555103222No Hit
CCCCAAAAGGCCTCCGTGCGGTGGAGTGAATCCCAGCCCGAATGCTCCAG5010.1294145605587816No Hit
CGGTGGTCTCCATGCGACGTGCAGAGGTGAAGCGAAGTGCACACGGTCCG4980.12863962307040566No Hit
ATATAACTGAAAGCCAAACAGTGGGGGAAAGCCCTACGAACCACTGAACA4960.12812299807815503No Hit
CAGTAAACCCTGTTCCGACTACTGTCTCTCACATATCGTCAATCTTCTCG4940.12760637308590442No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN4900.12657312310140315No Hit
CTTCTAGATACCGCCTCAGCTCTGTATCGGGATGCCTTAGAGTCTCCTGA4840.12502324812465127No Hit
GTTTACTAGTGCCATTTGTTCAGTGGTTCGTAGGGCTTTCCCCCACTGTT4830.12476493562852597No Hit
CCTTATGAGTCCAAGGAATACTAACATTGAGATTCCCGAGATTTAGATCT4800.12398999814015002No Hit
GTTCATGTCCTACTGTTCAAGCCTCCAAGCTGTGCCTTGGGTGGCTTTAG4680.12089024818664627No Hit
GTCCGGCAGATGAGAAGGCACAGACGGGGAGTCCGCGTAAAGAGAGGTGC4650.12011531069827035No Hit
ATGTATATTTGCGGGAGAGAACAACAGAGTTATCCGTCCCGAGAATGTTT4640.11985699820214503No Hit
AATATACATCGTTTCCATGGCTGCTAGGCTGTGCTGCCAACTGGATCCTG4610.11908206071376908No Hit
CTTATATAATATACCCGCCTTCCATAGAGTGTGTAAATAATGTCTGGTTT4610.11908206071376908No Hit
ACCGTGTGTCTTGGCCAAAATTCGCAGTCCCCAACCTCCAATCACTCACC4570.11804881072926783No Hit
GGTTTAAATGTATACCCAAAGACAAAAGAAAATTGGTAACAGCGGTAAAA4540.1172738732408919No Hit
GGCCTATTGATTGGAAAGTTTGTCAACGTATTGTGGGTCTTTTGGGTTTT4530.1170155607447666No Hit
CGGTAGTTCCCCCTAGAAAATTGAGAGAAGTCCACCACGAGTCTAGACTC4490.11598231076026533No Hit
CTTCTACTGTTCCTGTCTTTAACCCTCATTGGAAAACACCCTCTTTTCCT4470.11546568576801472No Hit
ATTTATGCCTACAGCCTCCGAGTACAAAGACCTTTAATCTAAGCTCCTCC4440.11469074827963877No Hit
GAATACTAACATTGAGATTCCCGAGATTTAGATCTTCTGCGACGCGGCGA4420.11417412328738816No Hit
CCTGTATTCCCATCCCATCATCCTGGGCTTTCGGAAAATTCCTATGGGAG4350.11236593581451096No Hit
CCTCTATGTATCCCTCCTGTTGCTGTACCAAACCTTCGGACGGAAATTGC4340.11210762331838565No Hit
GTACCAAACCTTCGGACGGAAATTGCACCTGTATTCCCATCCCATCATCC4330.11184931082226034No Hit
CCATATAACTGAAAGCCAAACAGTGGGGGAAAGCCCTACGAACCACTGAA4330.11184931082226034No Hit
GTCCAAGGAATACTAACATTGAGATTCCCGAGATTTAGATCTTCTGCGAC4330.11184931082226034No Hit
GTTTACGTCCCGTCGGCGCTGAATCCTGCGGACGACCCTTCTCGGGGCCG4270.11029943584550846No Hit
GCTTTAATGCCTTTGTATGCATGTATTCAATCTAAGCAGGCTTTCACTTT4210.10874956086875658No Hit
GACGGGGAGTCCGCGTAAAGAGAGGTGCGCCCCGTGGTCGGACGAAACGG4180.10797462338038065No Hit
GTCTTGGTGTAAATGTATATTAGGAAAAGAGGGTGTTTTCCAATGAGGGT4160.10745799838813003No Hit
GAATACATGCATACAAAGGCATTAAAGCAGGATAACCACATTGTGTAAAA4140.1069413733958794No Hit
ATGTGGTATTGGGGGCCAAGTCTGTACAGCATCTTGAGTCCCTTTTTACC4080.10539149841912754No Hit
GTATTAAACCTTATTATCCAGAACATCTAGTTAATCATTACTTCCAAACC4040.10435824843462627No Hit
CTCTAAGGCATCCCGATACAGAGCTGAGGCGGTATCTAGAAGATCTCGTA4030.10409993593850095No Hit
GGTGGAGACAGAGGGGTAGGCTGCCTTCCTGACTGGCGATTGGTAGAGGC3990.1030666859539997No Hit
TTCCCCCACTGTTTGGCTTTCAGTTATATGGATGATGTGGTATTGGGGGC3980.10280837345787439No Hit
CATTACAGAGAGTCCAAGAGTCCTCTTATGTAAGACCTTGGGCAAGAATT3970.10255006096174908No Hit
GTGTAAATAATGTCTGGTTTGGAAGTAATGATTAACTAGATGTTCTGGAT3900.10074187348887191No Hit
CCATATTCTTGGGAACAAGAGCTACAGCATGGGGCAGAATCTTTCCACCA3890.10048356099274659No Hit
GCACGGGACCATGCAGAACCTGCACGACTCCTGCTCAAGGAACCTCTATG3880.10022524849662127No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)