FastQCFastQC Report
Fri 2 Sep 2022
EGAF00002490294

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002490294
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences305729
Sequences flagged as poor quality0
Sequence length35-151
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGCTGGTGCGCAGACCAATTTATGCCTACAGCCTCCTAGTACAAAGACC20770.6793598252046747No Hit
TCCATAGAGTGTGTAAATAATGTCTGGTTTGGAAGTAATGATTAACTAGA17550.5740377916390005No Hit
CTCTATGGAAGGCGGGTATATTATATAAGAGAGAAACAACACATAGCGCC15210.49749941942046716No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN11770.3849814705180078No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGGAACTACCGTG11040.36110411508231144No Hit
CCCAAAGTGAGAGGCCTGTATTTCCCTGCTGGTGGCTCCAGTTCAGGGAC10780.3525998515024744No Hit
CACTCTGGGATCTTGCAGAGTTTGGTGGAAGGTTGTGGAGTTCCACTGCA9970.32610579958067437No Hit
AGTCTAGACTCTGCGGTATTGTGAGGATTCTTGTCAACAAGAAAAACCCC9570.3130223171501559No Hit
GTATCTCCCTGCTGGTGGCTCCAGTTCAGGGACAGTAAACCCTGTTCCGA7990.26134256154960767No Hit
GCCTTGTATCGGGAAGCCTTAGAGTCTCCTGAGCATTGTTCACCTCACCA7990.26134256154960767No Hit
GCTCTGTATCGGGAAGCCTTAGAGTCTCCTGAGCATTGTTCACCTCACCA7870.25741751682045205No Hit
GTCCCGTGCTGGTGGTTGAAGATCCTGGAATTAGAGGACAAACGGGCAAC7420.24269859908611874No Hit
CCTCCAGCTTATAGACCACCAAATGCCCCTATCTTATCAACACTTCCGGA6990.22863385547331133No Hit
GTATCGGGAAGCCTTAGAGTCTCCTGAGCATTGTTCACCTCACCATACTG6670.2181670695288965No Hit
CTCTTCGGTATTGTGAGGATTCTTGTCAACAAGAAAAACCCCGCCTGTAA6410.20966280594905945No Hit
ATACAGAGCTGAAGCGGTATCCAGAAGATCTCGTACAGAAGGAAAGAAGT6280.20541067415914094No Hit
GGGAAATACAGGCCTCTCACTCTGGGATCTTGCAGAGTTTGGTGGAAGGT6150.20115854236922243No Hit
GTGTAAAAGGGGCAGCAAAACCCAAAAGACCCACAATACGTTGACAGACT6110.19985019412617056No Hit
TCCTCAGGCCATGCAGTGGAACTCCACAACCTTCCACCAAACTCTGCAAG6100.1995231070654076No Hit
ACCGCAGAGTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGGAA5900.19298136585014836No Hit
GTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATTACAGAGAGTCCAA5710.18676671169565204No Hit
ATGTAAGACCTTGGGCAAGAAGTGGTGGGCGTTCACGGTGGTCTCCATGC5580.1825145799057335No Hit
ACTGTGGAGTTACTCTCGTTTTTGCCTACTGACTTCTTTCCTTCTGTACG5500.1798978834196298No Hit
AGATAAGGGTATTAAACCTTATTATCCAGAACATCTAGTTAATCATTACT5430.17760827399428905No Hit
CATTTACACCAAGACATTATCAAAAAATGTGAACAATTTGTAGGCCCACT5370.17564575162971127No Hit
GTACAGACTTGGCCCCCAATACCACATCATCCATATAACTGAAAGCCAAA5350.17499157750818536No Hit
GTATTTCCCTGCTGGTGGCTCCAGTTCAGGGACAGTAAACCCTGTTCCGA5320.17401031632589647No Hit
TTTCTGGCTCCTCTGCCGATCCATACTGCGGAACTCCTAGCCGCTTGTTT5280.1727019680828446No Hit
CTGTAAGACCGTTTCTCTTCCAAAAGTGAGACAAGAAATGTGAAACCACA5130.16779566217140016No Hit
GTCCAAGAGTCCTCTTATGTAAGACCTTGGGCAAGAAGTGGTGGGCGTTC5030.16452479156377053No Hit
ACATAGAGGTTCCTTGAGCAGTAGTCGTGCAGGTTTTGCATGGTCCCGTG4970.16256226919919275No Hit
GCCTAATCATCTCTGTTCATGTCCTACTGTTCAAGCCTCCAAGCTGTGCC4970.16256226919919275No Hit
GCACGGGACCATGCAAAACCTGCACGACTACTGCTCAAGGAACCTCTATG4930.16125392095614088No Hit
ATATTAGTGTTAACATAACTAACTACTAGGTCCCTGGATGTTGGATCTTC4930.16125392095614088No Hit
GTCTTACAGTATTTGGTGTCTTTCGGAGTGTGGATTCGCACTCCTCCAGC4850.1586372244700372No Hit
GTATTAAACCTTATTATCCAGAACATCTAGTTAATCATTACTTCCAAACC4790.15667470210545942No Hit
ATATAAGAGAGAAACAACACATAGCGCCTCATTCTGTGGGTCACCATATT4780.15634761504469644No Hit
CACTAATATGGGCCTAAAGTTCAGGCAACTATTGTGGTTTCACATTTCTT4770.15602052798393348No Hit
CTCTTATATAATATACCCGCCTTCCATAGAGTGTGTAAATAATGTCTGGT4770.15602052798393348No Hit
CTTATATAATATACCCGCCTTCCATAGAGTGTGTAAATAATGTCTGGTTT4590.15013296089020015No Hit
GTAGAAGCAGGAGGTGGATTTGCTGGCAAGGTTTGTAGTATGCCCTGAGC4510.14751626440409643No Hit
ACACATAGCGCCTCATTCTGTGGGTCACCATATTCTTGGGAACAAGAGCT4470.1462079161610446No Hit
GAGCTACATCATGGGGCAGAATCTTTCCACCAGCAATCCTCTGGGATTCT4430.14489956791799274No Hit
CTACAAACCTTGCCAGCAAATCCACCTCCTGCTTCTACCAATCGCCAGTC4380.14326413261417792No Hit
GGTTTGTAGTATGCCCTGAGCCTGAGGGCTCCACCCCAAAAGGCCTCCGT4270.13966617494578532No Hit
TACCTTGATAGTCCAGAAGAACCAACAAGAAGATGAGGCATAGCAGCAGG4260.13933908788502236No Hit
GCCTGAGGATGAGTGTTTCTCAAAGGTGGAGACAGGGGGGTAGGCTGCCT4030.1318160854874742No Hit
GCCTAGCAGCCATGGAAACGATGTATATTTGCGGGAGAGAACAACAGAGT4020.13148899842671125No Hit
GTTCATGTCCTACTGTTCAAGCCTCCAAGCTGTGCCTTGGGTGGCTTTGG4000.1308348243051853No Hit
ATACCACATCATCCATATAACTGAAAGCCAAACAGTGGGGGAAAGCCCTA3990.13050773724442236No Hit
ATACAAGGCTGAGGCGGTATCCAGAAGATCTCGTACAGAAGGAAAGAAGT3890.1272368666367927No Hit
GTCCAGAAGAACCAACAAGAAGATGAGGCATAGCAGCAGGATGAAGAGGA3880.12690977957602975No Hit
CTATGTATCCCTCCTGTTGCTGTACCAAACCTTCGGACGGAAATTGCACC3880.12690977957602975No Hit
GTAGAAGAGTAAAGCCCCGTAAAGCTTCCCACCTTATGAGTCCAAGGAAT3860.12625560545450382No Hit
TTATATAAGAGAGAAACAACACATAGCGCCTCATTCTGTGGGTCACCATA3850.12592851839374086No Hit
CATTTAAACCCTAACAAAACAAAAAGGTGGGGTTACTCTTTACATTTCAT3780.12363890896840012No Hit
ATCAAGGTATGTTGCCCGTTTGTCCTCTAATTCCAGGATCTTCAACCACC3730.1220034736645853No Hit
CTTTTACACAATGTGGTTATCCTGCTTTAATGCCTTTGTATGCATGTATT3730.1220034736645853No Hit
ACATTACAGAGAGTCCAAGAGTCCTCTTATGTAAGACCTTGGGCAAGAAG3660.11971386423924456No Hit
GTGTAAACAATACCTGAACCTTTACCCCGTTGCCCGGCAACGGCCAGGTC3630.11873260305695567No Hit
CCTTATGAGTCCAAGGAATACTAACATTGAGATTCCCGAGATTGAGATCT3600.11775134187466678No Hit
ATGTGGTATTGGGGGCCAAGTCTGTACAGCATCTTGAGTCCCTTTTTACC3560.11644299363161492No Hit
GTGTAAATGTATATTAGGAAAAGAGGGTGTTTTCCAATGAGGGTTAAAGA3560.11644299363161492No Hit
CTAGTAAACTGAGCCAGGAGAAACGGGCTGAGGCCCACTCCCATAGGAAT3550.11611590657085197No Hit
GGGTAAAGGTTCAGGTATTGTTTACACAGAAAGGCCTTATAAGTTGGCGA3490.11415338420627419No Hit
ATACTGCACTCAGGCAAGCAATTCTTTGCTGGGGGGAACTAATGACTCTA3480.11382629714551122No Hit
AGAGCTACATCATGGGGCAGAATCTTTCCACCAGCAATCCTCTGGGATTC3450.11284503596322233No Hit
TCCTAGTACAAAGACCTTTAATCTAATCTCCTCCCCCAACTCCTCCCAGT3420.11186377478093344No Hit
AGCCCGAAAGGTTCCACGCATGCGCTGATGGCCCATGACCAAGCCCCAGC3360.10990125241635566No Hit
GTCCGGCAGATGAGAAGGCACAGACGGGGAGTCCGCGTAAAGAGAGGTGC3330.10891999123406677No Hit
CAATAGGCCTGTTAACAGGAAGTTTTCTAAAACATTCTTTGATTTTCTGT3290.10761164299101492No Hit
AGTCTGTACAGCATCTTGAGTCCCTTTTTACCGCTGTTACCAATTTTCTT3280.10728455593025195No Hit
AGCTGGAGGAGTGCGAATCCACACTCCGAAAGACACCAAATACTGTAAGA3240.1059762076872001No Hit
CCTCTATGTATCCCTCCTGTTGCTGTACCAAACCTTCGGACGGAAATTGC3230.10564912062643714No Hit
GCCTTAGAGTCTCCTGAGCATTGTTCACCTCACCATACTGCACTCAGGCA3170.10368659826185936No Hit
TCTCTGTTCATGTCCTACTGTTCAAGCCTCCAAGCTGTGCCTTGGGTGGC3170.10368659826185936No Hit
TCCTTGGACTCATAAGGTGGGAAGCTTTACGGGGCTTTACTCTTCTACTG3070.10041572765422972No Hit
GTTTAAATGTATACCCAAAGACAAAAGAAAATTGGTAACAGCGGTAAAAA3060.10008864059346677No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)