FastQCFastQC Report
Fri 2 Sep 2022
EGAF00002490296

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002490296
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences256500
Sequences flagged as poor quality0
Sequence length35-151
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCCACAGAGTGTGTAAATAATGTCTGGCTTGGAAGTAATGATTAACTAAA20350.7933723196881092No Hit
CTCTATGGAAGGCGGGTATATTATATAAGAGAGAAACAACACATAGCGCC18710.7294346978557505No Hit
CCCCAAAAGGCCTCCGTGAGGTGGGGTGAATCCCAGCCCGAATGCTCCAG10590.41286549707602344No Hit
CTCTGTGGAAGGCGGGTATATTATATAAGAGAGAAACAACACATAGCGCC10500.40935672514619886No Hit
CCTCCAGCTTATAGACCACCAAATGCCCCTATCTTATCAACACTTCCGGA10340.4031189083820662No Hit
GTGCTGGTGCGCAGACCAATTTATGCCTACAGCCTCCTAGTACAAAGACC8240.3212475633528265No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGGAACCACCGTG8230.32085769980506823No Hit
TCCATAGAGTGTGTAAATAATGTCTGGCTTGGAAGTAATGATTAACTAAA8220.32046783625730996No Hit
ACTACATGTTCTGGATAATAAGGTTTGATACCCTTATCTAATGGCAAATA8030.31306042884990254No Hit
AGTCTAGACTCTGCGGTATTGTGAGGATTCTTGTCAACAAGAAAAACCCC6700.2612085769980507No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN6150.23976608187134502No Hit
CCTTCTGGGGTGGAGCCCTCAGGCTCAGGGCATACTACAAACCTTGCCAG5790.22573099415204678No Hit
CTTTAGGGCATGGACATTGACCCTTATAAAGAATTTGGAGCTACTGTGGA5780.22534113060428848No Hit
TCTCAATGTTAGTATTCCTTGGACTCATAAGGTGGGAAACTTTACGGGGC5720.2230019493177388No Hit
CATTTACACCAAGACATTATCAAAAAATGTGAACAATTTGTAGGCCCACT5350.20857699805068228No Hit
CTACTGTACCTGTCTTTAACCCACATTGGAAAACACCCTCTTTTCCTAAT5310.20701754385964913No Hit
GTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATTACAGAGAGTCCAA5250.20467836257309943No Hit
CCCCAGAAGGCCTCCGTGAGGTGGGGTGAATCCCAGCCCGAATGCTCCAG4930.19220272904483432No Hit
ATGTAAGACCTTGGGCAAGAATCGGTGGGCGTTCACGGTGGTCTCCATGC4930.19220272904483432No Hit
GTGTAAAAGGGGCAGCAAACCCCAAAAGACCCACAATACGTTGACAAACT4790.18674463937621832No Hit
GTATAATGTGATCTTGTGGCAAGGACCCATAACATCCAATAACATAGCCC4720.18401559454191033No Hit
TCCTCAGGCCATGCAGTGGAACTCCACAACTTTCCACCAAACTCTGCAAG4620.18011695906432748No Hit
GTACAGACTTGGCCCCCAATACCACATCATCCATATAACTGAAAGCCAAA4530.1766081871345029No Hit
GTCATAGAGTATTTGGTGTCTTTCGGAGTGTGGATTCGCACTCCTCCAGC4310.16803118908382067No Hit
GCCTAGCAGCCATGGAAACGATGTGTATTTGCGGGAGAGAACAACAGAGT4310.16803118908382067No Hit
CTCTTACTCTGGGATCTTGCAGAGTTTGGTGGAAAGTTGTGGAGTTCCAC4250.16569200779727095No Hit
CCCTTATCTAATGGCAAATATTTGGTAACATTTGGATAAAACCTAGCAGG4190.16335282651072125No Hit
GTCCAAGAGTCCTCTTATGTAAGACCTTGGGCAAGAATCGGTGGGCGTTC4190.16335282651072125No Hit
ACATCGAGATTCCCGAGATTGAGATCTTCTGCGACGCGGCGATTGAGACC4140.16140350877192983No Hit
GACCTTTAATCTAATCTCCTCCCCCAACTCCTCCCAGTCTTTAAACAAAC4100.15984405458089668No Hit
ATGTTATTGGATGTTATGGGTCCTTGCCACAAGATCACATTATACAGAAA4060.15828460038986356No Hit
ATTTCCGTCCGAAGGTTTGGTACAGCAACAGGAGGGATACATAGAGGTTC4000.15594541910331383No Hit
TCCACAGAGTGTGTAAATAATGTCTGGCTTGGAAGTAATGATTAACTACA3990.15555555555555556No Hit
ATATAAGAGAGAAACAACACATAGCGCCTCATTTTGTGGGTCACCATATT3920.15282651072124756No Hit
ACTAAATGTTCTGGATAATAAGGTTTGATACCCTTATCTAATGGCAAATA3920.15282651072124756No Hit
CTACAAACCTTGCCAGCAAATCCGCCTCCTGCCTCTACCAATCGCCAGTC3860.15048732943469786No Hit
GGCTTTCACTTTCTCGCCAACTTACAAGGCCTTTCTGTGTAAACAATACC3790.14775828460038987No Hit
GCTTTAGGGCATGGACATTGACCCTTATAAAGAATTTGGAGCTACTGTGG3710.1446393762183236No Hit
CTTCTGGGGTGGAGCCCTCAGGCTCAGGGCATACTACAAACCTTGCCAGC3690.14385964912280702No Hit
AGATAAGGGTATCAAACCTTATTATCCAGAACATTTAGTTAATCATTACT3680.14346978557504875No Hit
GCCTAATCATCTCTTGTTCATGTCCTACTGTTCAAGCCTCCAAGCTGTGC3660.14269005847953214No Hit
ACATTACAGAGAGTCCAAGAGTCCTCTTATGTAAGACCTTGGGCAAGAAT3630.14152046783625732No Hit
ATACCACATCATCCATATAACTGAAAGCCAAACAGTGGGGGAAAGCCCTA3400.13255360623781678No Hit
GAGAAACACTCATCCTCAGGCCATGCAGTGGAACTCCACAACTTTCCACC3380.13177387914230018No Hit
TACCTTGATAGTCCAGAAGAACCAACAAGAAGATGAGGCATAGCAGCAGG3370.1313840155945419No Hit
GGGTAAAGGTTCAGGTATTGTTTACACAGAAAGGCCTTGTAAGTTGGCGA3310.12904483430799218No Hit
GGATAACCACATTGTGTAAAAGGGGCAGCAAACCCCAAAAGACCCACAAT3260.1270955165692008No Hit
CATCGAGATTCCCGAGATTGAGATCTTCTGCGACGCGGCGATTGAGACCT3240.12631578947368421No Hit
GCACGGGACCATGCAGAACCTGCACGACTATTGCTCAAGGAACCTCTATG3230.1259259259259259No Hit
GGTTGGAATTAGAGGACAAACGGGCAACATACCTTGATAGTCCAGAAGAA3220.12553606237816764No Hit
GAGTAAGAGGCCTGTATTTCCCTGCTGGTGGCTCCAGTTCAGGAACAGTA3220.12553606237816764No Hit
GTGTCTCTCAAAGGTGGAGACAGCGGGGTAGGCTGCCTTCCTGACTGGCG3190.12436647173489279No Hit
CAATAACATAGCCCATGAAATTTAAAGAGTAACCCCATCTTTTTGTTTTG3110.12124756335282652No Hit
GGGTTTACTGTTCCTGAACTGGAGCCACCAGCAGGGAAATACAGGCCTCT3110.12124756335282652No Hit
GTACCAAACCTTCGGACGGAAATTGCACCTGTATTCCCATCCCATCATCC3100.12085769980506823No Hit
ACATAGAGGTTCCTTGAGCAATAGTCGTGCAGGTTCTGCATGGTCCCGTG3090.12046783625730995No Hit
ACTGTGGAGTTACTCTCGTTTTTGCCTTCTGACTTCTTTCCTTCAGTACG3060.1192982456140351No Hit
GCTTTGGGGCATGGACATTGACCCTTATAAAGAATTTGGAGCTACTGTGG3040.11851851851851852No Hit
GCCTTAGAGTCTCCTGAGCATTGTTCACCTCACCATACTGCTCTCAGGCA3040.11851851851851852No Hit
CTGTAATGTCAACGACCGACCTTGAGGCATACTTCAAAGACTGTTTGTTT3020.11773879142300193No Hit
CCCAAAAGGCCTCCGTGAGGTGGGGTGAATCCCAGCCCGAATGCTCCAGC2940.11461988304093566No Hit
GAGTCAGAGGCCTGTATTTCCCTGCTGGTGGCTCCAGTTCAGGAACAGTA2920.11384015594541912No Hit
GTATTTCCCTGCTGGTGGCTCCAGTTCAGGAACAGTAAACCCTGTTCCGA2920.11384015594541912No Hit
ATATTAGTGTTGACATAACTGACTACTAGGTCCCTAGATGCTGGATCTTC2900.11306042884990253No Hit
GCCTGAGGATGAGTGTTTCTCAAAGGTGGAGACAGCGGGGTAGGCTGCCT2900.11306042884990253No Hit
TTATATAAGAGAGAAACAACACATAGCGCCTCATTTTGTGGGTCACCATA2900.11306042884990253No Hit
GTGTAAATGTATATTAGGAAAAGAGGGTGTTTTCCAATGTGGGTTAAAGA2890.11267056530214424No Hit
CTCAACGTTAGTATTCCTTGGACTCATAAGGTGGGAAACTTTACGGGGCT2880.11228070175438595No Hit
GGTTCTGCATGGTCCCGTGCTGGTGGTTGAAGATCCTGGAATTTGAGGAC2850.1111111111111111No Hit
CACTAATATGGGCCTAAAGTTCAGACAATTATTGTGGTTTCACATTTCTT2830.11033138401559453No Hit
CTTTTACACAATGTGGTTATCCTGCTTTAATGCCTTTGTATGCCTGTATT2830.11033138401559453No Hit
CTAGTAAACTGAGCCAGGAGAAACGGGCTGAGGCCCACTCCCATAGGAAT2820.10994152046783627No Hit
CTCTAAGGCATCCCGATACAAAGCTGAGGCGGTATCTAGAAGATCTCGTA2810.10955165692007798No Hit
CTTTTGGGGTGGAGCCCTCAGGCTCAGGGCATACTACAAACCTTGCCAGC2790.1087719298245614No Hit
CTCTATGACCGTTTCTCTTCCAAAAGTGAGACAAGAAATGTGAAACCACA2780.10838206627680311No Hit
AACATCGAGATTCCCGAGATTGAGATCTTCTGCGACGCGGCGATTGAGAC2780.10838206627680311No Hit
ATTCCAGGATCTTCAACCACCAGCACGGGACCATGCAGAACCTGCACGAC2760.10760233918128655No Hit
TCCATAGAGTGTGTAAATAATGTCTGGCTTGGAAGTAATGATTAACTACA2760.10760233918128655No Hit
GTCCGGCAGATGAGAAGGCACAGACGGGGAGTCCGCGTAAAGAGAGGTGC2720.10604288499025341No Hit
CAATAGGCCTGTTAACAGGGAGTTTTCGAAAACATTCTTTGATTTTCTGT2700.10526315789473684No Hit
CAGTAAACCCTGTTCCGACTACTGCCTCTCCCATATCGTCAATCTTCTCG2700.10526315789473684No Hit
CTCCTAGTACAAAGACCTTTAATCTAATCTCCTCCCCCAACTCCTCCCAG2680.10448343079922029No Hit
CACCAAGACATTATCAAAAAATGTGAACAATTTGTAGGCCCACTCACAGT2680.10448343079922029No Hit
GTCCAGAAGAACCAACAAGAAGATGAGGCATAGCAGCAGGATGAAGAGGA2660.1037037037037037No Hit
GTATGGTGAGGTGAACAATGCTCAGGAGACTCTAAGGCATCCCGATACAA2630.10253411306042887No Hit
ACATTTAGTTAATCATTACTTCCAAGCCAGACATTATTTACACACTCTATGGA2630.10253411306042887No Hit
GGTTTGTAGTATGCCCTGAGCCTGAGGGCTCCACCCCAAAAGGCCTCCGT2610.10175438596491228No Hit
ACATTTAGTTAATCATTACTTCCAAGCCAGACATTATTTACACACTCTGT2610.10175438596491228No Hit
GTATGGATCGGCAGAGGAGCCAAAAAGGTTCCACGCATGCGCTGATGGCC2600.10136452241715399No Hit
CTCTTATATAATATACCCGCCTTCCACAGAGTGTGTAAATAATGTCTGGC2570.10019493177387914No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)