FastQCFastQC Report
Fri 2 Sep 2022
EGAF00002490297

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002490297
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences256500
Sequences flagged as poor quality0
Sequence length35-151
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCCACAGAGTGTGTAAATAATGTCTGGCTTGGAAGTAATGATTAACTAAA19210.7489278752436647No Hit
CTCTATGGAAGGCGGGTATATTATATAAGAGAGAAACAACACATAGCGCC16730.6522417153996102No Hit
CCTCCAGCTTATAGACCACCAAATGCCCCTATCTTATCAACACTTCCGGA11740.4576998050682261No Hit
CCCCAAAAGGCCTCCGTGAGGTGGGGTGAATCCCAGCCCGAATGCTCCAG10580.4124756335282651No Hit
CTCTGTGGAAGGCGGGTATATTATATAAGAGAGAAACAACACATAGCGCC9350.3645224171539961No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGGAACCACCGTG8720.3399610136452242No Hit
TCCATAGAGTGTGTAAATAATGTCTGGCTTGGAAGTAATGATTAACTAAA8630.3364522417153996No Hit
ACTACATGTTCTGGATAATAAGGTTTGATACCCTTATCTAATGGCAAATA8390.3270955165692008No Hit
GTGCTGGTGCGCAGACCAATTTATGCCTACAGCCTCCTAGTACAAAGACC7000.27290448343079926No Hit
CTTTAGGGCATGGACATTGACCCTTATAAAGAATTTGGAGCTACTGTGGA6740.2627680311890838No Hit
AGTCTAGACTCTGCGGTATTGTGAGGATTCTTGTCAACAAGAAAAACCCC6340.24717348927875243No Hit
CTACTGTACCTGTCTTTAACCCACATTGGAAAACACCCTCTTTTCCTAAT6200.24171539961013647No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN6130.23898635477582847No Hit
CCCCAGAAGGCCTCCGTGAGGTGGGGTGAATCCCAGCCCGAATGCTCCAG5720.2230019493177388No Hit
CCTTCTGGGGTGGAGCCCTCAGGCTCAGGGCATACTACAAACCTTGCCAG5670.22105263157894736No Hit
TCTCAATGTTAGTATTCCTTGGACTCATAAGGTGGGAAACTTTACGGGGC5160.20116959064327486No Hit
GAATCTTTCCACCAGCAATCCTCTGGGATTCTTTCCCGACCACCAGTTGG5120.1996101364522417No Hit
GTACAGACTTGGCCCCCAATACCACATCATCCATATAACTGAAAGCCAAA5050.19688109161793374No Hit
GTATAATGTGATCTTGTGGCAAGGACCCATAACATCCAATAACATAGCCC4960.19337231968810917No Hit
CATTTACACCAAGACATTATCAAAAAATGTGAACAATTTGTAGGCCCACT4860.1894736842105263No Hit
GTGTAAAAGGGGCAGCAAACCCCAAAAGACCCACAATACGTTGACAAACT4780.18635477582846005No Hit
CTACAAACCTTGCCAGCAAATCCGCCTCCTGCCTCTACCAATCGCCAGTC4640.18089668615984406No Hit
ATGTTATTGGATGTTATGGGTCCTTGCCACAAGATCACATTATACAGAAA4620.18011695906432748No Hit
GCCTAATCATCTCTTGTTCATGTCCTACTGTTCAAGCCTCCAAGCTGTGC4600.17933723196881093No Hit
GTCATAGAGTATTTGGTGTCTTTCGGAGTGTGGATTCGCACTCCTCCAGC4480.17465886939571149No Hit
ATTTCCGTCCGAAGGTTTGGTACAGCAACAGGAGGGATACATAGAGGTTC4440.17309941520467836No Hit
CCCTTATCTAATGGCAAATATTTGGTAACATTTGGATAAAACCTAGCAGG4390.17115009746588694No Hit
CTCTTACTCTGGGATCTTGCAGAGTTTGGTGGAAAGTTGTGGAGTTCCAC4270.16647173489278752No Hit
TCCTCAGGCCATGCAGTGGAACTCCACAACTTTCCACCAAACTCTGCAAG4240.16530214424951267No Hit
GAGTAAGAGGCCTGTATTTCCCTGCTGGTGGCTCCAGTTCAGGAACAGTA4150.1617933723196881No Hit
GTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATTACAGAGAGTCCAA4050.15789473684210525No Hit
ATATAAGAGAGAAACAACACATAGCGCCTCATTTTGTGGGTCACCATATT3970.15477582846003898No Hit
GCTTTAGGGCATGGACATTGACCCTTATAAAGAATTTGGAGCTACTGTGG3960.1543859649122807No Hit
TCCACAGAGTGTGTAAATAATGTCTGGCTTGGAAGTAATGATTAACTACA3880.15126705653021444No Hit
CTTCTGGGGTGGAGCCCTCAGGCTCAGGGCATACTACAAACCTTGCCAGC3860.15048732943469786No Hit
AGATAAGGGTATCAAACCTTATTATCCAGAACATTTAGTTAATCATTACT3840.1497076023391813No Hit
ACTAAATGTTCTGGATAATAAGGTTTGATACCCTTATCTAATGGCAAATA3830.14931773879142302No Hit
GCCTAGCAGCCATGGAAACGATGTGTATTTGCGGGAGAGAACAACAGAGT3820.14892787524366471No Hit
ACATCGAGATTCCCGAGATTGAGATCTTCTGCGACGCGGCGATTGAGACC3780.1473684210526316No Hit
GGCTTTCACTTTCTCGCCAACTTACAAGGCCTTTCTGTGTAAACAATACC3660.14269005847953214No Hit
GCTTTGGGGCATGGACATTGACCCTTATAAAGAATTTGGAGCTACTGTGG3640.1419103313840156No Hit
ATGTAAGACCTTGGGCAAGAATCGGTGGGCGTTCACGGTGGTCTCCATGC3610.14074074074074072No Hit
GAGAAACACTCATCCTCAGGCCATGCAGTGGAACTCCACAACTTTCCACC3610.14074074074074072No Hit
GAGTCAGAGGCCTGTATTTCCCTGCTGGTGGCTCCAGTTCAGGAACAGTA3560.1387914230019493No Hit
GTCCAAGAGTCCTCTTATGTAAGACCTTGGGCAAGAATCGGTGGGCGTTC3480.13567251461988306No Hit
GCACGGGACCATGCAGAACCTGCACGACTATTGCTCAAGGAACCTCTATG3480.13567251461988306No Hit
CATCGAGATTCCCGAGATTGAGATCTTCTGCGACGCGGCGATTGAGACCT3420.13333333333333333No Hit
GGATAACCACATTGTGTAAAAGGGGCAGCAAACCCCAAAAGACCCACAAT3410.13294346978557503No Hit
ACTGTGGAGTTACTCTCGTTTTTGCCTTCTGACTTCTTTCCTTCAGTACG3390.13216374269005848No Hit
GACCTTTAATCTAATCTCCTCCCCCAACTCCTCCCAGTCTTTAAACAAAC3380.13177387914230018No Hit
CTTTTACACAATGTGGTTATCCTGCTTTAATGCCTTTGTATGCCTGTATT3350.13060428849902536No Hit
GTGTCTCTCAAAGGTGGAGACAGCGGGGTAGGCTGCCTTCCTGACTGGCG3240.12631578947368421No Hit
ATACCACATCATCCATATAACTGAAAGCCAAACAGTGGGGGAAAGCCCTA3170.12358674463937622No Hit
CAATAACATAGCCCATGAAATTTAAAGAGTAACCCCATCTTTTTGTTTTG3120.12163742690058478No Hit
CTTTTGGGGTGGAGCCCTCAGGCTCAGGGCATACTACAAACCTTGCCAGC3120.12163742690058478No Hit
GCCTTAGAGTCTCCTGAGCATTGTTCACCTCACCATACTGCTCTCAGGCA3070.11968810916179336No Hit
CAGTAAACCCTGTTCCGACTACTGCCTCTCCCATATCGTCAATCTTCTCG3070.11968810916179336No Hit
GTATTTCCCTGCTGGTGGCTCCAGTTCAGGAACAGTAAACCCTGTTCCGA3070.11968810916179336No Hit
AGTCTGTACAGCATCTTGAGTCCCTTTTTACCGCTGTTACCAATTTTCTT3030.11812865497076022No Hit
ATATTAGTGTTGACATAACTGACTACTAGGTCCCTAGATGCTGGATCTTC2990.1165692007797271No Hit
GGGTTTACTGTTCCTGAACTGGAGCCACCAGCAGGGAAATACAGGCCTCT2970.11578947368421054No Hit
TCCATAGAGTGTGTAAATAATGTCTGGCTTGGAAGTAATGATTAACTACA2950.11500974658869396No Hit
GGATAATAAGGTTTGATACCCTTATCTAATGGCAAATATTTGGTAACATT2920.11384015594541912No Hit
TACCTTGATAGTCCAGAAGAACCAACAAGAAGATGAGGCATAGCAGCAGG2920.11384015594541912No Hit
GGGTAAAGGTTCAGGTATTGTTTACACAGAAAGGCCTTGTAAGTTGGCGA2880.11228070175438595No Hit
CTCTATGACCGTTTCTCTTCCAAAAGTGAGACAAGAAATGTGAAACCACA2870.1118908382066277No Hit
ACATTACAGAGAGTCCAAGAGTCCTCTTATGTAAGACCTTGGGCAAGAAT2840.11072124756335282No Hit
GTACCAAACCTTCGGACGGAAATTGCACCTGTATTCCCATCCCATCATCC2810.10955165692007798No Hit
TTATATAAGAGAGAAACAACACATAGCGCCTCATTTTGTGGGTCACCATA2800.10916179337231968No Hit
CTCTTATATAATATACCCGCCTTCCACAGAGTGTGTAAATAATGTCTGGC2760.10760233918128655No Hit
CCCAAAAGGCCTCCGTGAGGTGGGGTGAATCCCAGCCCGAATGCTCCAGC2750.10721247563352826No Hit
ATTCCAGGATCTTCAACCACCAGCACGGGACCATGCAGAACCTGCACGAC2750.10721247563352826No Hit
TCTCTTGTTCATGTCCTACTGTTCAAGCCTCCAAGCTGTGCCTTGGGTGG2740.10682261208576997No Hit
ACATAGAGGTTCCTTGAGCAATAGTCGTGCAGGTTCTGCATGGTCCCGTG2730.10643274853801168No Hit
CACTAATATGGGCCTAAAGTTCAGACAATTATTGTGGTTTCACATTTCTT2730.10643274853801168No Hit
CTCAACGTTAGTATTCCTTGGACTCATAAGGTGGGAAACTTTACGGGGCT2720.10604288499025341No Hit
CAATAGGCCTGTTAACAGGGAGTTTTCGAAAACATTCTTTGATTTTCTGT2720.10604288499025341No Hit
GTGTAAATGTATATTAGGAAAAGAGGGTGTTTTCCAATGTGGGTTAAAGA2720.10604288499025341No Hit
GAACAAATGGCACTAGTAAACTGAGCCAGGAGAAACGGGCTGAGGCCCAC2680.10448343079922029No Hit
CTATGTATCCCTCCTGTTGCTGTACCAAACCTTCGGACGGAAATTGCACC2660.1037037037037037No Hit
TCCATAGAGTGTGTAAATAATGTCTGGCTTGGAAGTAATGATTAACTAAATGT2630.10253411306042887No Hit
CTGTAATGTCAACGACCGACCTTGAGGCATACTTCAAAGACTGTTTGTTT2600.10136452241715399No Hit
GACGCAAATTGCACCTGTATTCCCATCCCATCATCCTGGGCTTTCGGAAA2590.1009746588693957No Hit
CACCAAGACATTATCAAAAAATGTGAACAATTTGTAGGCCCACTCACAGT2570.10019493177387914No Hit
ACATTTAGTTAATCATTACTTCCAAGCCAGACATTATTTACACACTCTGT2570.10019493177387914No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)