FastQCFastQC Report
Thu 1 Sep 2022
EGAF00002490298

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002490298
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences111245
Sequences flagged as poor quality0
Sequence length35-151
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTTTCGGGAAGCCTTAGAGTCTCCTGAGCATTGTTCACCTCATCATACTG10810.9717290664748978No Hit
CTTATATAAGACCTTGGGCAAGAATTGGTGGGCGTTCACGGTGGTCTCCA10210.9177940581599172No Hit
CCTCAAGCTTATAGACCACCAAATGCCCCTATCTTATCAACGCTTCCGGA8240.7407074475257315No Hit
GTGGACTTACTCTCATTTTTGCCTTCTGACTTCTTTCCTTCCGTACGAGA7720.6939637736527484No Hit
TTTACCGCTGTTACCAATTTTCTTTTGTCTTTGGGTATACATTTAAACCC7140.6418265989482673No Hit
TTTTACCGCTGTTACCAATTTTCTTTTGTCTTTGGGTATACATTTAAACC6920.6220504292327745No Hit
TCTACCCTCCTCCCCCAACTCCTCCCAGTCTTTAAACAAACAGTCTTTGA6670.5995775091015326No Hit
GCCAAAAAGGTTCCACGCATGCGCTGATGGCCCATGACCAAGCCCCAGCC5350.4809204908085757No Hit
GACCTGGCCGTTGCCGGGCAACGGGGTAAAGGTTCAGGTATTGTTTACAC4750.4269854824935952No Hit
GTGCTGGTGCGCAGACCAATTTATGCCTACAGCCTCCTAGTACAAAGATC4690.42159198166209716No Hit
TCCCTAAACAGAGCTGAGGCGGTATCTAGAAGATCTCGTACGGAAGGAAA4150.37305047417861475No Hit
GTGGCACACCATATTCTTGGGAACAAGAGCTACAGCATGGGGCAGAATCT3910.3514764708526226No Hit
CTACCCTCCTCCCCCAACTCCTCCCAGTCTTTAAACAAACAGTCTTTGAA3870.3478808036316239No Hit
TCCATAGAGTGTGTAAATAATGTCTGGTTTGGAAGTAATGATTAACTAGA3810.34248730280012585No Hit
CTGGTTTCTTGCAGAGTTTGGTGGAAGGTTGTGGAGTTCCACTGCATGGC3800.3415883859948762No Hit
CAGCATTAAAAAGGGCCTCAAGGTGCTGTACAGACTTGGCCCCCAATACC3800.3415883859948762No Hit
GTGCACCACCTTGAGGCCCTTTTTACCGCTGTTACCAATTTTCTTTTGTC3720.3343970515528788No Hit
GCATTAAAAAGGGCCTCAAGGTGCTGTACAGACTTGGCCCCCAATACCAC3670.3299024675266304No Hit
GAACCACCGTGTGTCTTGGCCAAAATTCGCAGTCCCCAACCTCCAATCAC3440.30922738100588787No Hit
GTTTAGGGACGCCTTAGAGTCTCCTGAGCATTGTTCACCTCATCATACTG3430.30832846420063825No Hit
GTATATAAGAGAGTAACAACACATAGCGCCTCATTCTGTGGGACACCATA3360.3020360465638905No Hit
GTCAAGAAGGCAGCCAACCCCTCTGTCTCCACCTTTGAGAGACACCCATC3150.2831587936536473No Hit
CTTCTACTGTTCCTGTCTTTAACCCTCATTGGAAAACACCCTCTTTTCCT3020.2714728751854016No Hit
GGTGCTGTACAGACTTGGCCCCCAATACCACATCATCCATATAACTGAAA3000.26967504157490224No Hit
CTTACACAAGAGGACTCTTGGACTCTCTGTGATGTCAACGACCGACCTTG2990.26877612476965257No Hit
GGCCCGGTCTGTGCCAAGTGTTTGCTGACGCAACCCCCACTGGCTGGGGC2940.2642815407434042No Hit
GAATATCAGCAGTGAGATTCCCGAGATTGAGATCTTCTGCGACGCGGCGA2910.2615847903276552No Hit
GTGCTGGTGGGAGAGTAGCAGTGAGGACGACCAGAGTCACTCTCATCGCA2780.2498988718594094No Hit
GTGGACTTACTCTCGTTTTTGCCTTCTGACTTCTTTCCTTCCGTACGAGA2720.24450537102791137No Hit
CGGTCGTTCCCCCTAGAAAATTGAGAGAAGTCCACCACGAGTCTAGACTC2720.24450537102791137No Hit
GCTAATATTCCTTGGACTCATAAGGTGGGAAACTTTACGGGGCTTTACTC2610.23461728617016495No Hit
CGGTAGTTCCCCCTAGAAAATTGAGAGAAGTCCACCACGAGTCTAGACTC2510.2256281181176682No Hit
CTTCCTGACTGGCGATTGGTAGAAGCAGGAGGCGGATTTGTTGGCAAGGT2480.22293136770191918No Hit
GTTATTAGACGACGAGGCAGGTCCCCTAGAAGAAGAACTCCCTCGCCTCG2460.22113353409141984No Hit
GGCTTTCACTTTCTCGCCAACTTACAAGGCCTTTCTGTGTAAACAATACC2460.22113353409141984No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN2460.22113353409141984No Hit
CTTATATACTATACCTGCCTTCCATAGAGTGTGTAAATAATGTCTGGTTT2460.22113353409141984No Hit
CTCTCTTATATACTATACCCGCCTTCCATAGAGTGTGTAAATAATGTCTG2420.21753786687042112No Hit
CTCTATCTCCACCTTTGAGAGACACCCATCCTCAGGCCATGCAGTGGAAC2380.21394219964942246No Hit
ACACCAATATGGGCCTAAAGTTCAGGCAACTATTGTGGTTTCACATTTCT2370.2130432828441728No Hit
TCCCGAAACAGAGCTGAGGCGGTATCTAGAAGATCTCGTACGGAAGGAAA2370.2130432828441728No Hit
CTTTAGGATAAAACCTAGCAGGCATAATCAATTGCAGTCTTCTTTTCTCA2250.2022562811811767No Hit
ATACCCGCCTTCCATAGAGTGTGTAAATAATGTCTGGTTTGGAAGTAATG2240.20135736437592702No Hit
CCTCAAGCTTATAGACCACCAAATGCCCCTATCTTATCGACGCTTCCGGA2180.19596386354442896No Hit
GATTTCAATCCCAACAAGGACACCTGGCCAGACGCCAACAAGGTAGGAGC2110.18967144590768126No Hit
CCCTAGATGGTTGATCTTCCAAATTACCACCCACCCAGGTGGATAGATTT2030.18248011146568385No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGGAACCACCGTG1990.17888444424468514No Hit
ATATTCTACTCTCCTCCCCCAACTCCTCCCAGTCTTTAAACAAACAGTCT1960.17618769382893612No Hit
AGCTTGAGGAGTGCGAATCCACACTCCGAAAGACACCAAATACTCTATGA1930.1734909434131871No Hit
AGTCTAGACTCTGCGGTATTGTGAGGATTCTTGTCAACAAGAAAAACCCC1880.16899635938693874No Hit
ACCCTTCTCGGGGCCGCTTGGGACTCTCTCGTCCCCTTCTCCGTCTGCCG1820.16360285855544068No Hit
TCTAACAACAGTAGTCTCCGGAAGCGTTGATAAGATAGGGGCATTTGGTG1800.16180502494494134No Hit
ATAAAAGAGTAACAACACATAGCGCCTCATTCTGTGGGACACCATATTCT1780.160007191334442No Hit
CTTTTGGATAAAACCTAGCAGGCATAATCAATTGCAGTCTTCTTTTCTCA1740.1564115241134433No Hit
CTTACATAAGAGGACTCTTGGACTCTCTGTGATGTCAACGACCGACCTTG1740.1564115241134433No Hit
ATCCTAAAGTTACCAAATATTTGCCATTGGATAAGGGTATTAAACCTTAT1620.1456245224504472No Hit
GAAGATGAGGCATAGCAGCAGGATGAAGAGGAAGATGATAAAACGCCGCA1610.14472560564519754No Hit
CTCCCATAGGAATTTTCCGAAAGCCCAGGATGATGGGATGGGAATACAGG1580.14202885522944853No Hit
TTAGAGGACAAACGGGCAACATACCCTGATAGTCCAGAAGAACCAACAAG1570.14112993842419885No Hit
GTGTAAAAGGGGCAGCAAAACCCAAAAGACCCACAATACGTTGACAGACT1560.14023102161894915No Hit
CTCCCGCTCCTACCTTGTTGGCGTCTGGCCAGGTGTCCTTGTTGGGATTG1520.13663535439795046No Hit
AGATCCCAGAGTGAGCCATGCTGGTGGCTCCAGTTCAGGAACAGTAAACC1510.1357364375927008No Hit
GAATCTCTCCACCAGCAATCCTCTGGGATTCTTTCCCGACCACCAGTTGG1500.13483752078745112No Hit
CTCTCTTATATACTATACCTGCCTTCCATAGAGTGTGTAAATAATGTCTG1490.13393860398220145No Hit
ATATAAGACCTTGGGCAAGAATTGGTGGGCGTTCACGGTGGTCTCCATTC1490.13393860398220145No Hit
CTCTTATATACTATACCTGCCTTCCATAGAGTGTGTAAATAATGTCTGGT1490.13393860398220145No Hit
GGTTTGTAGTATGCCCTGAGCCTGAGGGCTCCACCCCAAAAGGCCTCCGT1490.13393860398220145No Hit
CTCCTCAAGCTTATAGACCACCAAATGCCCCTATCTTATCAACGCTTCCG1480.13303968717695178No Hit
GTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATCACAGAGAGTCCAA1460.13124185356645243No Hit
TATATAAGACCTTGGGCAAGAATTGGTGGGCGTTCACGGTGGTCTCCATT1410.12674726954020407No Hit
GGGTTTACTGTTCCTGAACTGGAGCCACCAGCAGGGCTCACTCTGGGATC1410.12674726954020407No Hit
ATAAAAGAGTAACAACACATAGCGCCTCATTCTGTGGCACACCATATTCT1400.12584835273495437No Hit
GAGATTCCCGAGATTGAGATCTTCTGCGACGCGGCGATTGAGACCTTCGT1390.1249494359297047No Hit
CCTTTCTGGCTCCTCTACCGATTCATACTGCGGAACTCCTAGCCGCTTGT1380.12405051912445504No Hit
CTCTATGGAAGGCAGGTATAGTATATAAGAGAGTAACAACACATAGCGCC1360.12225268551395568No Hit
CTTTCTGGCTCCTCTACCGATTCATACTGCGGAACTCCTAGCCGCTTGTT1360.12225268551395568No Hit
GTGTCCCACAGAATGAGGCGCTATGTGTTGTTACTCTCTTATATACTATA1360.12225268551395568No Hit
GTGAATTTACTCTCGTTTTTGCCTTCTGACTTCTTTCCTTCCGTACGAGA1350.12135376870870603No Hit
AGTCTAGACTCTGTGGTATTGTGAGGATTCTTGTCAACAAGAAAAACCCC1350.12135376870870603No Hit
CAGTAAACCCTGTTCCGACTACTGTCTCTCACATATCGTCAATCTTCTCG1340.12045485190345634No Hit
GTGCTGTACAGACTTGGCCCCCAATACCACATCATCCATATAACTGAAAG1340.12045485190345634No Hit
CTTATATACTATACCCGCCTTCCATAGAGTGTGTAAATAATGTCTGGTTT1340.12045485190345634No Hit
GCCTTAGAGTCTCCTGAGCATTGTTCACCTCATCATACTGCACTCAGGCA1320.11865701829295698No Hit
GGAATATGATAAAACGCCGCAGACACATCCAGCGATAACCAGGACAAGTT1310.11775810148770731No Hit
AGATCCCAGAGTGAGCCCTGCTGGTGGCTCCAGTTCAGGAACAGTAAACC1310.11775810148770731No Hit
CGGTCATAGAGTATTTGGTGTCTTTCGGAGTGTGGATTCGCACTCCTCAA1300.11685918468245765No Hit
GTCTTTCGGAGTGTGGATTCGCACTCCTCAAGCTTATAGACCACCAAATG1270.11416243426670862No Hit
CACTAATATGGGCCTAAAGTTCAGGCAACTATTGTGGTTTCACATTTCTT1260.11326351746145893No Hit
CTCTTATATACTATACCCGCCTTCCATAGAGTGTGTAAATAATGTCTGGT1230.11056676704570992No Hit
CGGTGGTTCCCCCTAGAAAATTGAGAGAAGTCCACCACGAGTCTAGACTC1230.11056676704570992No Hit
CTTGTGTAAGACCTTGGGCAAGAATTGGTGGGCGTTCACGGTGGTCTCCA1220.10966785024046025No Hit
GACCGGGCCGTTGCCGGGCAACGGGGTAAAGGTTCAGGTATTGTTTACAC1210.10876893343521056No Hit
ACATAGAGGTTCCTTGAGCAGGAGTCGTGCAGGTTCTGCATGGTCCCGTG1210.10876893343521056No Hit
CTGCGGTATTGTGAGGATTCTTGTCAACAAGAAAAACCCCGCCTGTAACA1200.1078700166299609No Hit
GAGACGGGTCGTCCGCAGGATTCAGCGCCGACGGGACGTAAACAAAGGAC1200.1078700166299609No Hit
GAGCTGGAGCATTCGGGCTGGGATTCACCCCACCGCACGGAGGCCTTTTG1190.10697109982471123No Hit
TCCTCAGGCCATGCAGTGGAACTCCACAACCTTCCACCAAACTCTGCAAG1180.10607218301946154No Hit
CTACAAACCTTGCCAACAAATCCGCCTCCTGCTTCTACCAATCGCCAGTC1180.10607218301946154No Hit
CTTTCGGAGTGTGGATTCGCACTCCTCAAGCTTATAGACCACCAAATGCC1180.10607218301946154No Hit
GCCTAGCAGCCATGGAAACGATGTATATTTACGGGAGAGAACAACAGAGT1180.10607218301946154No Hit
GCTGATATTCCTTGGACTCATAAGGTGGGAAACTTTACGGGGCTTTACTC1170.10517326621421189No Hit
CATCCAGGGACCTAGTAGTCAGTTATGTTAACACTAATATGGGCCTAAAG1150.10337543260371253No Hit
GTCTTACATAAGAGGACTCTTGGACTCTCTGTGATGTCAACGACCGACCT1140.10247651579846286No Hit
CTTTTACACAATGTGGTTATCCTGCTTTAATGCCTTTGTATGCATGTATT1130.10157759899321317No Hit
ATGTTTGATCTTCCAAATTACCACCCACCCAGGTGGATAGATTTAGTAGG1130.10157759899321317No Hit
ATATAAGAGAGTAACAACACATAGCGCCTCATTCTGTGGGACACCATATT1120.10067868218796351No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)