FastQCFastQC Report
Thu 1 Sep 2022
EGAF00002490299

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002490299
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences111245
Sequences flagged as poor quality0
Sequence length35-151
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTTTCGGGAAGCCTTAGAGTCTCCTGAGCATTGTTCACCTCATCATACTG10310.926783226212414No Hit
CTTATATAAGACCTTGGGCAAGAATTGGTGGGCGTTCACGGTGGTCTCCA8280.7443031147467302No Hit
GTGGACTTACTCTCATTTTTGCCTTCTGACTTCTTTCCTTCCGTACGAGA7340.6598049350532608No Hit
CCTCAAGCTTATAGACCACCAAATGCCCCTATCTTATCAACGCTTCCGGA7280.6544114342217627No Hit
TTTTACCGCTGTTACCAATTTTCTTTTGTCTTTGGGTATACATTTAAACC5720.5141804126028136No Hit
TTTACCGCTGTTACCAATTTTCTTTTGTCTTTGGGTATACATTTAAACCC5630.5060901613555665No Hit
TCTACCCTCCTCCCCCAACTCCTCCCAGTCTTTAAACAAACAGTCTTTGA4950.4449638185985887No Hit
GCCAAAAAGGTTCCACGCATGCGCTGATGGCCCATGACCAAGCCCCAGCC4840.43507573374084224No Hit
GTGCTGGTGCGCAGACCAATTTATGCCTACAGCCTCCTAGTACAAAGATC4550.4090071463886017No Hit
GACCTGGCCGTTGCCGGGCAACGGGGTAAAGGTTCAGGTATTGTTTACAC4460.40091689514135465No Hit
GAACCACCGTGTGTCTTGGCCAAAATTCGCAGTCCCCAACCTCCAATCAC4160.3739493909838644No Hit
GTGCACCACCTTGAGGCCCTTTTTACCGCTGTTACCAATTTTCTTTTGTC3650.3281046339161311No Hit
TCCATAGAGTGTGTAAATAATGTCTGGTTTGGAAGTAATGATTAACTAGA3630.3263068003056317No Hit
CTGGTTTCTTGCAGAGTTTGGTGGAAGGTTGTGGAGTTCCACTGCATGGC3560.320014382668884No Hit
GTTTAGGGACGCCTTAGAGTCTCCTGAGCATTGTTCACCTCATCATACTG3410.30653063059013885No Hit
TCCCTAAACAGAGCTGAGGCGGTATCTAGAAGATCTCGTACGGAAGGAAA3370.3029349633691402No Hit
GTCAAGAAGGCAGCCAACCCCTCTGTCTCCACCTTTGAGAGACACCCATC3210.2885522944851454No Hit
ACCCTTCTCGGGGCCGCTTGGGACTCTCTCGTCCCCTTCTCCGTCTGCCG3150.2831587936536473No Hit
GTGGCACACCATATTCTTGGGAACAAGAGCTACAGCATGGGGCAGAATCT3140.2822598768483977No Hit
GAATATCAGCAGTGAGATTCCCGAGATTGAGATCTTCTGCGACGCGGCGA2980.2678772079644029No Hit
GTGGACTTACTCTCGTTTTTGCCTTCTGACTTCTTTCCTTCCGTACGAGA2960.26607937435390355No Hit
GTATATAAGAGAGTAACAACACATAGCGCCTCATTCTGTGGGACACCATA2950.2651804575486539No Hit
CAGCATTAAAAAGGGCCTCAAGGTGCTGTACAGACTTGGCCCCCAATACC2950.2651804575486539No Hit
GCATTAAAAAGGGCCTCAAGGTGCTGTACAGACTTGGCCCCCAATACCAC2930.26338262393815454No Hit
GGCCCGGTCTGTGCCAAGTGTTTGCTGACGCAACCCCCACTGGCTGGGGC2850.25619128949615716No Hit
CTACCCTCCTCCCCCAACTCCTCCCAGTCTTTAAACAAACAGTCTTTGAA2820.25349453908040814No Hit
GTGCTGGTGGGAGAGTAGCAGTGAGGACGACCAGAGTCACTCTCATCGCA2580.23192053575441593No Hit
CTTATATACTATACCTGCCTTCCATAGAGTGTGTAAATAATGTCTGGTTT2580.23192053575441593No Hit
CTTTAGGATAAAACCTAGCAGGCATAATCAATTGCAGTCTTCTTTTCTCA2530.22742595172816757No Hit
GCTAATATTCCTTGGACTCATAAGGTGGGAAACTTTACGGGGCTTTACTC2530.22742595172816757No Hit
GATTTCAATCCCAACAAGGACACCTGGCCAGACGCCAACAAGGTAGGAGC2520.22652703492291787No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN2460.22113353409141984No Hit
CTTACACAAGAGGACTCTTGGACTCTCTGTGATGTCAACGACCGACCTTG2430.21843678367567082No Hit
CTTCCTGACTGGCGATTGGTAGAAGCAGGAGGCGGATTTGTTGGCAAGGT2370.2130432828441728No Hit
GGTGCTGTACAGACTTGGCCCCCAATACCACATCATCCATATAACTGAAA2270.20405411479167604No Hit
CGGTCGTTCCCCCTAGAAAATTGAGAGAAGTCCACCACGAGTCTAGACTC2250.2022562811811767No Hit
CGGTAGTTCCCCCTAGAAAATTGAGAGAAGTCCACCACGAGTCTAGACTC2240.20135736437592702No Hit
GAATCTCTCCACCAGCAATCCTCTGGGATTCTTTCCCGACCACCAGTTGG2230.20045844757067732No Hit
CTTCTACTGTTCCTGTCTTTAACCCTCATTGGAAAACACCCTCTTTTCCT2200.1977616971549283No Hit
GGCTTTCACTTTCTCGCCAACTTACAAGGCCTTTCTGTGTAAACAATACC2140.19236819632343027No Hit
GTTATTAGACGACGAGGCAGGTCCCCTAGAAGAAGAACTCCCTCGCCTCG2110.18967144590768126No Hit
TCCCGAAACAGAGCTGAGGCGGTATCTAGAAGATCTCGTACGGAAGGAAA2070.18607577868668254No Hit
CTCTATCTCCACCTTTGAGAGACACCCATCCTCAGGCCATGCAGTGGAAC2020.18158119466043418No Hit
ATATTCTACTCTCCTCCCCCAACTCCTCCCAGTCTTTAAACAAACAGTCT2000.17978336104993484No Hit
CTCTCTTATATACTATACCCGCCTTCCATAGAGTGTGTAAATAATGTCTG1960.17618769382893612No Hit
AGCTTGAGGAGTGCGAATCCACACTCCGAAAGACACCAAATACTCTATGA1940.1743898602184368No Hit
ATACCCGCCTTCCATAGAGTGTGTAAATAATGTCTGGTTTGGAAGTAATG1830.16450177536069036No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGGAACCACCGTG1820.16360285855544068No Hit
ACACCAATATGGGCCTAAAGTTCAGGCAACTATTGTGGTTTCACATTTCT1800.16180502494494134No Hit
CCCTAGATGGTTGATCTTCCAAATTACCACCCACCCAGGTGGATAGATTT1730.15551260730819363No Hit
ATCCTAAAGTTACCAAATATTTGCCATTGGATAAGGGTATTAAACCTTAT1690.15191694008719492No Hit
CCTCAAGCTTATAGACCACCAAATGCCCCTATCTTATCGACGCTTCCGGA1660.1492201896714459No Hit
GAACAAATGGCACTAGTAAACTGAGCCAGGAGAAACGGGCTGAGGCCCAC1640.14742235606094656No Hit
GGTTTGTAGTATGCCCTGAGCCTGAGGGCTCCACCCCAAAAGGCCTCCGT1610.14472560564519754No Hit
GTGTAAAAGGGGCAGCAAAACCCAAAAGACCCACAATACGTTGACAGACT1590.14292777203469817No Hit
CTTTTGGATAAAACCTAGCAGGCATAATCAATTGCAGTCTTCTTTTCTCA1590.14292777203469817No Hit
GTGAATTTACTCTCGTTTTTGCCTTCTGACTTCTTTCCTTCCGTACGAGA1540.13843318800844984No Hit
CTTATATACTATACCCGCCTTCCATAGAGTGTGTAAATAATGTCTGGTTT1540.13843318800844984No Hit
CCTTTCTGGCTCCTCTACCGATTCATACTGCGGAACTCCTAGCCGCTTGT1540.13843318800844984No Hit
ATAAAAGAGTAACAACACATAGCGCCTCATTCTGTGGGACACCATATTCT1530.13753427120320014No Hit
GAGACGGGTCGTCCGCAGGATTCAGCGCCGACGGGACGTAAACAAAGGAC1500.13483752078745112No Hit
TCTAACAACAGTAGTCTCCGGAAGCGTTGATAAGATAGGGGCATTTGGTG1490.13393860398220145No Hit
CACTAATATGGGCCTAAAGTTCAGGCAACTATTGTGGTTTCACATTTCTT1480.13303968717695178No Hit
AGTCTAGACTCTGCGGTATTGTGAGGATTCTTGTCAACAAGAAAAACCCC1480.13303968717695178No Hit
GGGTTTACTGTTCCTGAACTGGAGCCACCAGCAGGGCTCACTCTGGGATC1450.13034293676120273No Hit
CTTACATAAGAGGACTCTTGGACTCTCTGTGATGTCAACGACCGACCTTG1450.13034293676120273No Hit
GTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATCACAGAGAGTCCAA1440.1294440199559531No Hit
CTCTCTTATATACTATACCTGCCTTCCATAGAGTGTGTAAATAATGTCTG1400.12584835273495437No Hit
CTCTTATATACTATACCTGCCTTCCATAGAGTGTGTAAATAATGTCTGGT1370.12315160231920536No Hit
AGATCCCAGAGTGAGCCATGCTGGTGGCTCCAGTTCAGGAACAGTAAACC1360.12225268551395568No Hit
CTCCCATAGGAATTTTCCGAAAGCCCAGGATGATGGGATGGGAATACAGG1330.11955593509820667No Hit
GCACGGGACCATGCAGAACCTGCACGACTCCTGCTCAAGGAACCTCTATG1330.11955593509820667No Hit
CTCTATGGAAGGCAGGTATAGTATATAAGAGAGTAACAACACATAGCGCC1330.11955593509820667No Hit
CTCCCGCTCCTACCTTGTTGGCGTCTGGCCAGGTGTCCTTGTTGGGATTG1310.11775810148770731No Hit
CTCCTCAAGCTTATAGACCACCAAATGCCCCTATCTTATCAACGCTTCCG1310.11775810148770731No Hit
GGAATATGATAAAACGCCGCAGACACATCCAGCGATAACCAGGACAAGTT1290.11596026787720796No Hit
GAGATTCCCGAGATTGAGATCTTCTGCGACGCGGCGATTGAGACCTTCGT1290.11596026787720796No Hit
ATAAAAGAGTAACAACACATAGCGCCTCATTCTGTGGCACACCATATTCT1280.11506135107195829No Hit
CTTTCTGGCTCCTCTACCGATTCATACTGCGGAACTCCTAGCCGCTTGTT1260.11326351746145893No Hit
GTGTCCCACAGAATGAGGCGCTATGTGTTGTTACTCTCTTATATACTATA1260.11326351746145893No Hit
GCCTTAGAGTCTCCTGAGCATTGTTCACCTCATCATACTGCACTCAGGCA1260.11326351746145893No Hit
GACCGGGCCGTTGCCGGGCAACGGGGTAAAGGTTCAGGTATTGTTTACAC1250.11236460065620928No Hit
TTAGAGGACAAACGGGCAACATACCCTGATAGTCCAGAAGAACCAACAAG1240.11146568385095959No Hit
AGTCTAGACTCTGTGGTATTGTGAGGATTCTTGTCAACAAGAAAAACCCC1240.11146568385095959No Hit
GAGTCAGAGGGGTTGGCTGCCTTCCTGACTGGCGATTGGTAGAAGCAGGA1240.11146568385095959No Hit
CAGTAAACCCTGTTCCGACTACTGTCTCTCACATATCGTCAATCTTCTCG1230.11056676704570992No Hit
GCCTAGCAGCCATGGAAACGATGTATATTTACGGGAGAGAACAACAGAGT1230.11056676704570992No Hit
GGATAACCACATTGTGTAAAAGGGGCAGCAAAACCCAAAAGACCCACAAT1220.10966785024046025No Hit
CTTTTACACAATGTGGTTATCCTGCTTTAATGCCTTTGTATGCATGTATT1210.10876893343521056No Hit
GTGCTGTACAGACTTGGCCCCCAATACCACATCATCCATATAACTGAAAG1180.10607218301946154No Hit
ATGTGATCTTGTGGCAATGACCCATAACTTCCAATTACATAGCCCATAAA1170.10517326621421189No Hit
GTCTTACATAAGAGGACTCTTGGACTCTCTGTGATGTCAACGACCGACCT1170.10517326621421189No Hit
GCCTAATCATCTCTTGTTCATGTCCTACTGTTCAAGCCTCCAAGCTGTGC1150.10337543260371253No Hit
CGGTCATAGAGTATTTGGTGTCTTTCGGAGTGTGGATTCGCACTCCTCAA1130.10157759899321317No Hit
CTACAAACCTTGCCAACAAATCCGCCTCCTGCTTCTACCAATCGCCAGTC1130.10157759899321317No Hit
ACATAGAGGTTCCTTGAGCAGGAGTCGTGCAGGTTCTGCATGGTCCCGTG1130.10157759899321317No Hit
TATATAAGACCTTGGGCAAGAATTGGTGGGCGTTCACGGTGGTCTCCATT1120.10067868218796351No Hit
ATATAAGACCTTGGGCAAGAATTGGTGGGCGTTCACGGTGGTCTCCATTC1120.10067868218796351No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)