FastQCFastQC Report
Fri 2 Sep 2022
EGAF00002490301

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002490301
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences386978
Sequences flagged as poor quality0
Sequence length35-151
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGCTGGTGCGCAGACCAATTTATGCCTACAGCCTCCTAGTACAAAGACC25830.6674798050535172No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGGAACCACCGTG20740.5359477799771563No Hit
AGTCTAGACTCTGCGGTATTGTGAGGATTTTTGTCAACAAGAAAAACCCC13790.3563510070339916No Hit
ATATAGGAGAGAAACAACACGTAGCGCCTCATTTTGTGGGTCACCATATT12610.32585831752709454No Hit
TCCACAAAGTGTGTAAATAATGTCTGGTTTGGAAGTAATGATTAACTAAA10960.2832202347420267No Hit
TCCTCAGGCCATGCAGTGGAACTCCACAACTTTCCACCAAACACTGCAAG10800.27908563277498977No Hit
ATATAATACACCCGCCTTCCACAAAGTGTGTAAATAATGTCTGGTTTGGA10300.26616500162799955No Hit
GTGTAAAAGGGGCAGCAAAACCCAAAAGACCCACAATACGTTGACAGACT10270.2653897637591801No Hit
GTATTATATAGGAGAGAAACAACACGTAGCGCCTCATTTTGTGGGTCACC9800.2532443704810093No Hit
CTCTAAGACTACGTCTCTTCCAAAAGTGAGACAAGAAATGTGAAACCACA9740.25169389474337045No Hit
GTCTTAGAGTATTTGGTGTCTTTCGGAGTGTGGATTCGCACTCCTCCAGC9660.24962659375985197No Hit
ATACAAACCTTGCCAGCAAATCCGCCTCCTGCATCTACCAATCGCCAGTC8800.22740310818702872No Hit
ACTGTGGAGTTACTCTCATTTTTGCCTTCTGACTTCTTTCCTTCGGTACG8750.2261110450723297No Hit
CCTCCAGCTTATAGACCACCAAATGCCCCTATCTTATCAACACTTCCGGA8370.2162913654006171No Hit
GTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATTACAGAAAGTCCAA7830.2023370837618676No Hit
CATTTAAACCCTAACAAAACTAAAAGATGGGGTTACTCTTTAAATTTCAT7710.19923613228658993No Hit
GTACAGACTTGGCCCCCAATACCACATCATCCATATAACTGAAAGCCAAA7510.19406787982779383No Hit
GTCCCGCGCTGGTGGTTGAAGATCCTGGAATTAGAGGACAGACGGGCAAC7480.19329264195897441No Hit
GGTTTGTATTATGCCCTGAGCCTGAGGGCTCCACCCCAAAAGGCCTCCGT7450.192517404090155No Hit
TTGTACACCTCACCATACGGCACTCAGGCAAGCAGTACTTTGCTGGGGGG7160.18502343802490065No Hit
CTATGTATCCCTCCTGTTGCTGTACCAAACCTTCGGACGGAAATTGCACC7100.18347296228726181No Hit
CATTTACACCAAGACATTATCAAAAGATGTGAACAATTTGTAGGCCCACT7070.1826977244184424No Hit
GTATCGGGATGCCTTAGAGTCTCCTGAGCATTGTACACCTCACCATACGG7050.18218089917256278No Hit
CTCCTATATAATACACCCGCCTTCCACAAAGTGTGTAAATAATGTCTGGT6960.17985518556610453No Hit
GCCTTAGAGTCTCCTGAGCATTGTACACCTCACCATACGGCACTCAGGCA6920.1788215350743453No Hit
CCCTTATCTAATGGCAAATATTTGGTAACATTTGGATAAAACCTAGCAGG6910.17856312245140551No Hit
GCCTAGCAGCCATGGAAACGATGTATATTTGCGGGAGAGAACAACAGAGT6870.1775294719596463No Hit
GCCTAATCATCTCTTGTTCATGTCCTACTGTTCAAGCCTCCAAGCTGTGC6840.17675423409082686No Hit
CTAGTAAACTGAGCCAGGAGAAACGGGCTGAGGCCCACTCCCATAGGAAT6720.1736532826155492No Hit
GACTACGTCTCTTCCAAAAGTGAGACAAGAAATGTGAAACCACAATAGTT6690.1728780447467298No Hit
GCCTGAGGATGAGTGTTTCTCAAAGGTGGAGACAGCGGGGTAGGTTGCCT6630.17132756900909096No Hit
GTGTAAAGGGACTCAAGATGCTGTACAGACTTGGCCCCCAATACCACATC6380.16486725343559583No Hit
CTTTTACACAATGTGGTTATCCTGCTTTAATGCCTTTGTATGCCTGTATT6350.16409201556677638No Hit
GTTTACTAGTGCCCTTTGTTCAATGGTTCGTAGGGCTTTCCCCCACTGTT6230.16099106409149874No Hit
CTTTATGAGTCCAAGGAATACTAACATTGAGGTTCCCGAGATTGAGATCT6070.15685646212446186No Hit
AGATAAGGGTATTAAACCTTATTATCCGGAACATTTAGTTAATCATTACT6020.15556439900976282No Hit
CCTCTATGTATCCCTCCTGTTGCTGTACCAAACCTTCGGACGGAAATTGC6010.15530598638682303No Hit
GTGTAAATGTATATTAGGAAAAGAGGGTGTTTTCCAATGAGGGTTAAAGA5910.15272186015742498No Hit
GTATTAAACCTTATTATCCGGAACATTTAGTTAATCATTACTTCCAAACC5850.15117138441978614No Hit
ATACCACATCATCCATATAACTGAAAGCCAAACAGTGGGGGAAAGCCCTA5830.15065455917390652No Hit
TTCCCCCACTGTTTGGCTTTCAGTTATATGGATGATGTGGTATTGGGGGC5770.1491040834362677No Hit
GTGTAAACAATACCTGAACCTTTACCCCGTTGCTAGGCAACGGCCAGGTC5600.144711068846291No Hit
GTCCAAGGAATACTAACATTGAGGTTCCCGAGATTGAGATCTTCTGCGAC5600.144711068846291No Hit
GTATTATGCCCTGAGCCTGAGGGCTCCACCCCAAAAGGCCTCCGTGCGGT5570.1439358309774716No Hit
GTATGGATCGGCAGAGGAGCCAGAAAGGTTCCACGCATGCGCTGATGGCC5500.14212694261689296No Hit
ACATAGAGGTTCCTTGAGCAGTAGTCGTGCAGGTTCTGCATGGTCCCGCG5460.14109329212513372No Hit
GCGCGGGACCATGCAGAACCTGCACGACTACTGCTCAAGGAACCTCTATG5440.14057646687925412No Hit
ATGTGGTTATCCTGCTTTAATGCCTTTGTATGCCTGTATTCAATCTAAGC5440.14057646687925412No Hit
GGGTAAAGGTTCAGGTATTGTTTACACAGAAAGGCCTTGTAAGTTGGCGA5400.13954281638749488No Hit
GTTTAATACCCTTATCTAATGGCAAATATTTGGTAACATTTGGATAAAAC5270.13618345228927745No Hit
GTTCAATGGTTCGTAGGGCTTTCCCCCACTGTTTGGCTTTCAGTTATATG5190.134116151305759No Hit
AGCTGGAGGAGTGCGAATCCACACTCCGAAAGACACCAAATACTCTAAGA5130.13256567556812016No Hit
GTCCTACTGTTCAAGCCTCCAAGCTGTGCCTTGGGTGGCTTTAGGACATG5020.12972313671578228No Hit
GTCCGGCAGATGAGAAGGCACAGACGGGGAGTCCGCGTAAAGAGAGGTGC4980.12868948622402307No Hit
CTTCTAGATACCGCCTCAGCTCTGTATCGGGATGCCTTAGAGTCTCCTGA4950.12791424835520365No Hit
TTGTATGCCTGTATTCAATCTAAGCAGGCTTTCACTTTCTCGCCAACTTA4900.12662218524050464No Hit
CTTTAGGACATGGACATTGACCCTTATAAAGAATTTGGAGCTACTGTGGA4880.12610535999462502No Hit
GTCCAAGAGTCCTCTTATGTAAGACCTTTGGCAAGAATTGGTGGGCGTTC4860.12558853474874543No Hit
CCTTTACCCCGTTGCTAGGCAACGGCCAGGTCTGTGCCAAGTGTTTGCTG4840.1250717095028658No Hit
TAACCACATTGTGTAAAAGGGGCAGCAAAACCCAAAAGACCCACAATACG4790.12377964638816677No Hit
CCCCAAAAGGCCTCCGTGCGGTGGGGTGAATCCCAGCCCGAATGCTCCAG4740.12248758327346775No Hit
GAATACAGGCATACAAAGGCATTAAAGCAGGATAACCACATTGTGTAAAA4720.12197075802758814No Hit
CCTGTATTCCCATCCCATCATCCTGGGCTTTCGGAAAATTCCTATGGGAG4710.12171234540464831No Hit
GGACATCCTTTGTTTACGTCCCGTCGGCGCTGAATCCCGCGGACGACCCT4670.1206786949128891No Hit
ATGTGGTATTGGGGGCCAAGTCTGTACAGCATCTTGAGTCCCTTTACACC4660.1204202822899493No Hit
GAATACTAACATTGAGGTTCCCGAGATTGAGATCTTCTGCGACGCGGCGA4650.1201618696670095No Hit
GACCTGGCCGTTGCCTAGCAACGGGGTAAAGGTTCAGGTATTGTTTACAC4590.11861139392937066No Hit
TCCTAGTACAAAGACCTTTAATCTAGTCTCCTCCCCCAACTCCTCCCAGT4520.11680250556879203No Hit
GTTTAAATGTATGCCCAAAGACAAAAGAAAATTGGTAACAGCGGTGTAAA4510.1165440929458522No Hit
GTTATGTCAACACTCATATGGGCCTAAAGTTCAGGCAACTATTGTGGTTT4510.1165440929458522No Hit
AGTCTGTACAGCATCTTGAGTCCCTTTACACCGCTGTTACCAATTTTCTT4500.11628568032291242No Hit
GGATAACCACATTGTGTAAAAGGGGCAGCAAAACCCAAAAGACCCACAAT4440.11473520458527359No Hit
CCTTTACACCGCTGTTACCAATTTTCTTTTGTCTTTGGGCATACATTTAA4420.11421837933939397No Hit
GTATTTCCCTGCTGGTGGCTCCAGTTCAGGAACAGTAAACCCTGTTCCGA4420.11421837933939397No Hit
CTTTACACCGCTGTTACCAATTTTCTTTTGTCTTTGGGCATACATTTAAA4390.11344314147057456No Hit
GACATAGTCTTAGAGTATTTGGTGTCTTTCGGAGTGTGGATTCGCACTCC4380.11318472884763475No Hit
GGCATACAAAGGCATTAAAGCAGGATAACCACATTGTGTAAAAGGGGCAG4350.11240949097881534No Hit
ATGTAAGACCTTTGGCAAGAATTGGTGGGCGTTCACGGTGGTCTCCATGC4350.11240949097881534No Hit
CTTCTGGACTATCAGGGTATGTTGCCCGTCTGTCCTCTAATTCCAGGATC4310.11137584048705612No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN4300.1111174278641163No Hit
ACCCTGTTCCGACCACTGCCTCTCCCATATCGTCAATCTTCTCGAGGATT4270.1103421899952969No Hit
TCTAGGGACCTAGTGGTCAGTTATGTCAACACTCATATGGGCCTAAAGTT4270.1103421899952969No Hit
ATATGGGAGAGGCAGTGGTCGGAACAGGGTTTACTGTTCCTGAACTGGAG4250.10982536474941729No Hit
GTCTTGGTGTAAATGTATATTAGGAAAAGAGGGTGTTTTCCAATGAGGGT4230.10930853950353768No Hit
CTGCTAGGCTGTGCTGCCAACTGGATCCTACGCGGGACATCCTTTGTTTA4210.10879171425765806No Hit
GTTCATGTCCTACTGTTCAAGCCTCCAAGCTGTGCCTTGGGTGGCTTTAG4210.10879171425765806No Hit
CAGTAAACCCTGTTCCGACCACTGCCTCTCCCATATCGTCAATCTTCTCG4160.10749965114295902No Hit
GACATAGCCCATGAAATTTAAAGAGTAACCCCATCTTTTAGTTTTGTTAG4160.10749965114295902No Hit
CCCCTATCTTATCAACACTTCCGGAAACTACTGTTGTTAGACGACGAGGC4150.10724123852001922No Hit
CATCATACAGAAAATCAAAGAATGTTTTAGGAAACTTCCTGTTAACAGAC4120.10646600065119981No Hit
TCCTTGGACTCATAAAGTGGGTAACTTTACGGGGCTTTATTCTTCTACTT4120.10646600065119981No Hit
TCCATAAAGTGTGTAAATAATGTCTGGTTTGGAAGTAATGATTAACTAAA4110.10620758802826001No Hit
ATTATGCCCTGAGCCTGAGGGCTCCACCCCAAAAGGCCTCCGTGCGGTGG4110.10620758802826001No Hit
TCTCGGGGCCGCTTGGGGATCTTTCGTCCCCTTCTCCGTCTGCCGTTTCG4050.10465711229062118No Hit
CCTCAAGGTCGGTCGTTGACATTACAGAAAGTCCAAGAGTCCTCTTATGT4030.10414028704474156No Hit
CCCTGGGATCTTGCAGTGTTTGGTGGAAAGTTGTGGAGTTCCACTGCATG4030.10414028704474156No Hit
GGGTTTACTGTTCCTGAACTGGAGCCACCAGCAGGGAAATACAGGCCTCT4030.10414028704474156No Hit
CTCCCATATCGTCAATCTTCTCGAGGATTGGGGACCCTGCGCTGAACATG4010.10362346179886195No Hit
ATATCAGAGAGAAACAACACGTAGCGCCTCATTTTGTGGGTCACCATATT4010.10362346179886195No Hit
CAACAGATACAGCATGGGGCAGAATCTTTCCACCAGCAATCCTCTGGGAT4000.10336504917592215No Hit
ATGTATATTTGCGGGAGAGAACAACAGAGTTGTCCGTCCCGAGAATGTTT3980.10284822393004255No Hit
CTATAAGCTGGAGGAGTGCGAATCCACACTCCGAAAGACACCAAATACTC3960.10233139868416294No Hit
GTCCCCAATCCTCGAGAAGATTGACGATATGGGAGAGGCAGTGGTCGGAA3940.10181457343828332No Hit
CCATTGAACAAAGGGCACTAGTAAACTGAGCCAGGAGAAACGGGCTGAGG3910.1010393355694639No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)