FastQCFastQC Report
Fri 2 Sep 2022
EGAF00002490302

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002490302
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences308664
Sequences flagged as poor quality0
Sequence length35-151
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGCTGGTGCGCAGACCAATTTATGCCTACAGCCTCCTAGTACAAAGATC15370.49795246611201827No Hit
TCCATAGAGTGTGTAAATAATGTCTGGTTTGGAAGTAATGATTAACTAGA15050.4875852059197056No Hit
CCTCCAGCTTATAGACCACCAAATGCCCCTATCTTATCAACACTTCCGGA12430.4027032630951455No Hit
AGTCTAGACTCTGCGGTATTGTGAGGATTCTTGTCAACAAGAAAAACCCC9270.30032656869605784No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN9100.29481896171889177No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGGAACTACCGTG8680.28121193271648137No Hit
ATGTAAGACCTTGGGCAAGAATTGGTGGGCGTTCACGGTGGTCTCCATGC8590.27829614078739345No Hit
CTCTATGGAAGGCGGGTATATTATATAAGAGAGAAACAACACATAGCGCC8530.2763522795013348No Hit
CTACAAACCTTGCCAACAAATCCGCCTCCTGCCTCTACCAATCGCCAGTC8080.2617733198558951No Hit
GTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATTACAGAGAGTCCAA7160.23196744680299614No Hit
GGCTTTCACTTTCTCGCCAACTTACAAGGCCTTTCTGTGTAAACAATACC7060.22872767799289842No Hit
GGTTTGTAGTATGCCCTGAGCCTGAGGGCTCCACCCCAAAAGGCCTCCGT7020.22743177046885937No Hit
CATTTACACCAAGACATTATCAAAAAATGTGAACAATTTGTAGGCCCACT6460.20928906513231219No Hit
TCCTCAGGCCATGCAGTGGAACTCCACAACCTTCCACCAAACTCTGCAAG6380.206697250084234No Hit
GTCCAAGAGTCCTCTTATGTAAGACCTTGGGCAAGAATTGGTGGGCGTTC6350.20572531944120465No Hit
ATATAAGAGAGAAACAACACATAGCGCCTCATTCTGTGGGTCACCATATT6220.20151361998807768No Hit
ATATATGAGAGACAGTAGTCGGAACAGGGTTTACTGTTCCTGAACTGGAG6080.19697794365394083No Hit
CTCATATATCGTCAATCTTCTCGAGGATTGGGGACCCTGCGCTGAACATG5810.18823056786667702No Hit
GGATAAGGGTATTAAACCTTATTATCCAGAACATCTAGTTAATCATTACT5760.18661068346162818No Hit
CCCTTATCCAATGGCAAATATTTGGTAACATTTGGATAAAACCTAGCAGG5540.1794831920794132No Hit
TACCTTGATAGTCCAGAAGAACCAACAAGAAGATGAGGCATAGCAGCAGG5300.1717077469351787No Hit
CACTAATATGGGCCTAAAGTTCAGGCAAATATTGTGGTTTCACATTTCTT5160.16717207060104192No Hit
CATTTAAACCCTAACAAAACAAAAAGGTGGGGTTACTCTTTACATTTTAT5100.16522820931498328No Hit
CTATGTATCCCTCCTGTTGCTGTACCAAACCTTCGGACGGAAATTGCACC5040.16328434802892466No Hit
GTGTAAATGTATATTAGGAAAAGAGGGTGTTTTCCAATGAGGGTTAAAGA5010.16231241738589533No Hit
AGCTGGAGGAGTGCGAATCCACACTCCGAAAGACACCAAATACTCTATGA5000.1619884405048856No Hit
CTGTAATGTCAACGACCGACCTTGAGGCATACTTCAAAGACTGTTTGTTT4880.1581007179327683No Hit
ACATTACAGAGAGTCCAAGAGTCCTCTTATGTAAGACCTTGGGCAAGAAT4770.15453697224166082No Hit
ACATAGAGGTTCCTTGAGCAGGAGTCGTGCAGGTCCTGCATGGTCCCGTG4760.15421299536065106No Hit
CTCTATGACCGTTTCTCTTCCAAAAGTGAGACAAGAAATGTGAAACCACA4730.15324106471762175No Hit
GTGTAAACAATACCTGAACCTTTACCCCGTTGCCCGGCAACGGCCAGGTC4600.14902936526449473No Hit
GCCTAGCAGCCATGGAAACGACGTATATTTGCGGGAGAGAACAACAGAGT4560.14773345774045563No Hit
CAATACGTTGACAGACTTTCCAATCAATAGGCCTGTTAACAGGAAGTTTT4480.14514164269237745No Hit
ATCAAGGTATGTTGCCCGTTTGTCCTCTAATTCCAGGATCTTCAACCACC4350.14092994323925045No Hit
GTCCCGTGCTGGTGGTTGAAGATCCTGGAATTAGAGGACAAACGGGCAAC4340.14060596635824069No Hit
GTATTAAACCTTATTATCCAGAACATCTAGTTAATCATTACTTCCAAACC4250.13769017442915274No Hit
CTTCTACTGTTCCTGTCTTTAACCCTCATTGGAAAACACCCTCTTTTCCT4240.13736619754814294No Hit
GTCATAGAGTATTTGGTGTCTTTCGGAGTGTGGATTCGCACTCCTCCAGC4240.13736619754814294No Hit
CTCTTATATAATATACCCGCCTTCCATAGAGTGTGTAAATAATGTCTGGT4230.1370422206671332No Hit
GCCTAATCATCTCTTGTTCATGTCCTACTGTTCAAGCCTCCAAGCTGTGC4150.13445040561905502No Hit
GGGTAAAGGTTCAGGTATTGTTTACACAGAAAGGCCTTGTAAGTTGGCGA4140.13412642873804526No Hit
GAGCTACAGCATGGGGCAGAATCTTTCCACCAGCAATCCTCTGGGATTCT4120.1334784749760257No Hit
CCTTTACCCCGTTGCCCGGCAACGGCCAGGTCTATGCCAAGTGTTTGCTG4080.13218256745198662No Hit
CTTTTACACAATGTGGTTATCCTGCTTTAATGCCTTTGTATGCATGTATT4080.13218256745198662No Hit
CTAGTAAACTGAGCCAGGAGAAACGGGCTGAGGCCCACTCCCATAGGAAT4070.13185859057097687No Hit
GTATGGATCGGCAGAGGAGCCAGAAAGGTTCCACGCATGCGCTGATGGCC4050.1312106368089573No Hit
GTATCGGGAAGCCTTAGAATCTCCTGAGCATTGTTCACCTCACCATACTG4040.13088665992794754No Hit
GGGTTTACTGTTCCTGAACTGGAGCCACCAGCAGGGAAATACAGGCCTCT4010.12991472928491823No Hit
GTAGAAGAGTAAAGCCCCGTAAAGTTTCCCACCTTATGCGTCCAAGGAAT4000.12959075240390844No Hit
ATACCACATCATCCATATAACTGAAAGCCAAACAGTGGGGGAAAGCCCTA3980.12894279864188893No Hit
ACCCCTCTCGGGGCCGCTTGGGACTCTCTCGTCCCCTTCTCCGTCTGCCG3980.12894279864188893No Hit
GTCCGGCAGATGAGAAGGCACAGACGGGGAGTCCGCGTAAAGAGAGGTGC3970.12861882176087913No Hit
GGTCTATGCCAAGTGTTTGCTGACGCAACCCCCACTGGCTGGGGCTTGGT3950.12797086799885962No Hit
CCTCTATGTATCCCTCCTGTTGCTGTACCAAACCTTCGGACGGAAATTGC3940.12764689111784983No Hit
CGGTGGTCTCCATGCGACGTGCAGAGGTGAAGCGAAGTGCACACGGTCCG3840.12440712230775211No Hit
GTTTAATACCCTTATCCAATGGCAAATATTTGGTAACATTTGGATAAAAC3840.12440712230775211No Hit
CAATAGGCCTGTTAACAGGAAGTTTTCTAAAACATTCTTTGATTTTCTGT3830.12408314542674236No Hit
GCCTGAGGATGAGTGTTTCTCAATGGTGGAGACAGAGGGGTAGGCTGCCT3820.12375916854573257No Hit
GGGAAATACAGGCCTCTCACTCTGGGATCTTGCAGAGTTTGGTGGAAGGT3790.12278723790270327No Hit
GAGAAGGGTCGTCCGCAGGATTCAGCGCCGACGGGACGTAAACAAAGGAC3790.12278723790270327No Hit
GTCCAGAAGAACCAACAAGAAGATGAGGCATAGCAGCAGGATGAAGAGGA3760.12181530725967395No Hit
GTGCAGAGGTGAAGCGAAGTGCACACGGTCCGGCAGATGAGAAGGCACAG3750.12149133037866419No Hit
GTATTTCCCTGCTGGTGGCTCCAGTTCAGGAACAGTAAACCCTGTTCCGA3730.12084337661664463No Hit
GACGGGGAGTCCGCGTAAAGAGAGGTGCGCCCCGTGGTCGGTCGAAACGG3720.12051939973563486No Hit
CACCAAGACATTATCAAAAAATGTGAACAATTTGTAGGCCCACTCACAGT3670.11889951533058601No Hit
CAGTAAACCCTGTTCCGACTACTGTCTCTCATATATCGTCAATCTTCTCG3660.11857553844957625No Hit
ATATGAGAGACAGTAGTCGGAACAGGGTTTACTGTTCCTGAACTGGAGCC3570.1156597465204883No Hit
GTTTACTAGTGCCATTTGTTCAGTGGTTCGTAGGGCTTTCCCCCACTGTT3530.11436383899644921No Hit
GTATGCATGTATTCAATCTAAGCAGGCTTTCACTTTCTCGCCAACTTACA3520.11403986211543943No Hit
CATTCTCGGGACGGATAACTCTGTTGTTCTCTCCCGCAAATATACGTCGT3470.1124199777103906No Hit
CAAGAGCTACAGCATGGGGCAGAATCTTTCCACCAGCAATCCTCTGGGAT3460.11209600082938082No Hit
GTACAGACTTGGCCCCCAATACCACATCATCCATATAACTGAAAGCCAAA3460.11209600082938082No Hit
ATATTAGTGTTAACATAACTGACTACTAGGTCCCTGGATGTTTGATCTTC3430.11112407018635151No Hit
GTGTAAATAATGTCTGGTTTGGAAGTAATGATTAACTAGATGTTCTGGAT3420.11080009330534174No Hit
GCCTTAGAATCTCCTGAGCATTGTTCACCTCACCATACTGCACTCAGGCA3420.11080009330534174No Hit
ATTTATGCCTACAGCCTCCTAGTACAAAGATCATTAATCTAGTCTCCTCC3400.11015213954332218No Hit
GGATAACCACATTGTGTAAAAGGGGCAGCAAAACCCAAAAGACCCACAAT3380.10950418578130265No Hit
CTCTCATATATCGTCAATCTTCTCGAGGATTGGGGACCCTGCGCTGAACA3380.10950418578130265No Hit
ATATAACTGAAAGCCAAACAGTGGGGGAAAGCCCTACGAACCACTGAACA3360.1088562320192831No Hit
GTGTAAAAGGGGCAGCAAAACCCAAAAGACCCACAATACGTTGACAGACT3350.10853225513827335No Hit
GTTTAAATGTATACCCAAAGACAAAAGAAAATTGGTAACAGCGGTAAAAA3350.10853225513827335No Hit
CCCCAAAAGGCCTCCGTGCGGTGGGGTGAATCCCAGCCCGAATGCTCCAG3340.10820827825726356No Hit
ATACTGCACTCAGGCAAGCAATTCTTTGCTGGGGGGAACTAATGAATCTA3330.1078843013762538No Hit
GCTTTAGGGCATGGACCTTGATCCTTATAAAGAATTTGGAGCTACTGTGG3310.10723634761423426No Hit
CTTCTGGACTATCAAGGTATGTTGCCCGTTTGTCCTCTAATTCCAGGATC3290.1065883938522147No Hit
GCACGGGACCATGCAGGACCTGCACGACTCCTGCTCAAGGAACCTCTATG3280.10626441697120492No Hit
CCTGTATTCCCATCCCATCATCCTGGGCTTTCGGAAAATTCCTATGGGAG3270.10594044009019517No Hit
CATTACAGAGAGTCCAAGAGTCCTCTTATGTAAGACCTTGGGCAAGAATT3260.1056164632091854No Hit
TCTAGCTACCGCCTCAGCTCTGTATCGGGAAGCCTTAGAATCTCCTGAGC3220.10432055568514631No Hit
GGTGGAGACAGAGGGGTAGGCTGCCTTCCTGACTGGCGATTGGTAGAGGC3200.10367260192312676No Hit
ACCCTTCTCGGGGCCGCTTGGGACTCTCTCGTCCCCTTCTCCGTCTGCCG3150.10205271751807792No Hit
CATTTAGATTCCCGAGATTGAGATCTTCTGCGACGCGGCGATTGAGACCT3140.10172874063706815No Hit
GTATCTCTTGTTCCCAAGAATATGGTGACCCACAGAATGAGGCGCTATGT3130.10140476375605836No Hit
CCGATAATGTTTGCTCCAGACCTGCTGCGAGCAAAACAAGCGGCTAGGAG3130.10140476375605836No Hit
GTCTTGGTGTAAATGTATATTAGGAAAAGAGGGTGTTTTCCAATGAGGGT3130.10140476375605836No Hit
GCTTTAATGCCTTTGTATGCATGTATTCAATCTAAGCAGGCTTTCACTTT3130.10140476375605836No Hit
GCATAGACCTGGCCGTTGCCGGGCAACGGGGTAAAGGTTCAGGTATTGTT3110.10075680999403884No Hit
CTTATATAATATACCCGCCTTCCATAGAGTGTGTAAATAATGTCTGGTTT3090.10010885623201929No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)