FastQCFastQC Report
Fri 2 Sep 2022
EGAF00002490304

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002490304
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences391164
Sequences flagged as poor quality0
Sequence length35-151
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCCATAGAGTGTGTAAATAATGTCTGGTTTGGAAGTAATGATTAACTAGA24300.6212228119152069No Hit
GTGCTGGTGCGCAGACCAATTTATGCCTACAGCCTCCTAGTACAAAGATC18990.48547412338558765No Hit
CTCTATGGAAGGCGGGTGTATTATATCAGAGAGAAACAACACATAGCGCC18330.4686014050372733No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGGAACTACCGTG14270.3648086224703705No Hit
AGTCTAGACTCTGCGGTATTGTGAGGATTCTTGTCAACAAGAAAAACCCC13190.3371987197185835No Hit
CTACAAACCTTGCCAACAAATCCGCCTCCTGCCTCGACCAATCGCCAGTC10780.27558773302246625No Hit
ATGTAAGACCTTGGGCAAGAATTGGTGGGCGTTCACGGTGGTCTCCATGC10180.2602488981603624No Hit
ATTTCCGTCCGAAGGTTTTGTACAGCAACAGGAGGGATACATAGAGGTTC9760.2495117137568897No Hit
GTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATTACAGGGAGTCCAA9730.2487447720137845No Hit
CATTTACACCAAGACATTATCAAAAAATGTGAACAATTTGTAGGCCCACT9490.24260923806894297No Hit
GTGTAAAAGGGGCAGCAAAACCCAAAAGACCCACAATACGTTGACAGACT9090.2323833481608737No Hit
ATATTAGTGTTAACATAACTGACTACTAGGTCCCTGGATGTTGGATCTTG7820.19991614770275384No Hit
CTCTATGACCGTTTCTCTTCCAAAAGTGAGACAAGAAATGTGAAACCACA7640.1953144972441227No Hit
GTACAGACTTGGCCCCCAATACCACATCATCCATATAACTGAAAGCCAAA7550.19301367201480707No Hit
GTATTATATCAGAGAGAAACAACACATAGCGCCTCATTCTGTGGGTCACC7540.19275802476710535No Hit
CCTCCAGCTTATAGACCACCAAATGCCCCTATCTTATCAACACTTCCGGA7440.19020155229008806No Hit
GGTTTGTAGTATGCCCTGAGCCTGAGGGCTCCACCCCAAAAGGCCTCCGT7430.18994590504238631No Hit
GTTTAATACCCTTATCCAATGGCAAATATTTGGTAACCTTTGGATAAAAC7430.18994590504238631No Hit
CACTAATATGGGCCTAAAGTTCAGGCAACTATTGTGGTTTCACATTTCTT7280.18611119632686035No Hit
CCCTTATCCAATGGCAAATATTTGGTAACCTTTGGATAAAACCTAGCAGG7020.17946436788661532No Hit
GGATAAGGGTATTAAACCGTATTATCCAGAACATCTAGTTAATCATTACT6970.17818613164810668No Hit
CATTTAAACCCTAACAAAACAAAAAGGTGGGGTTACTCTTTACATTTTAT6930.17716354265729975No Hit
GCCTAATCATCTCTTGTTCATGTCCTACTGTTCAAGCCTCCAAGCTGTGC6750.1725618921986686No Hit
GTCCAAGAGTCCTCTTATGTAAGACCTTGGGCAAGAATTGGTGGGCGTTC6710.17153930320786165No Hit
GTCATAGAGTATTTGGTGTCTTTCGGAGTGTGGATTCGCACTCCTCCAGC6690.1710280087124582No Hit
TCACCATACTGCACTCAGGCAAGCAATTCTTTGCTGGGGGGAACTAATGA6500.1661707110061253No Hit
TCCTCAGGCCACGCAGTGGAACTCCACAACCTTCCACCAAACTCTGCAAG6400.163614238529108No Hit
GTAGAAGAGTAAAGCCCCGTAAAGTTTCCCACCTTATGAGTCCAAGGAAT6360.16259164953830108No Hit
ATACCACATCATCCATATAACTGAAAGCCAAACAGTGGGGGAAAGCCCTA6330.1618247077951959No Hit
ACATAGAGGTTCCTTGAGCAGGAGTCGTGCAGGTTCTGCATGGTCCCGTG6250.159779529813582No Hit
TACCTTGATAGTCCAGAAGAACCAACAAGAAGATGAGGCATAGCAGCAGG6230.15926823531817857No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN6000.15338834862103876No Hit
CACTCACAGTCAATGAAAAAAGAAGACTGAAATTGATTATGCCTGCTAGG5980.1528770541256353No Hit
TCTGTGGAGTTACTCTCGTTTTTGCCTTCTGACTTCTTTCCTAACGTACG5960.15236575963023183No Hit
GTCCGGCAGATGAGAAGGCACAGACGGGGAGTCCGCGTAAAGAGAGGTGC5890.1505762288963197No Hit
GGGTAAAGGTTCAGGTATTGTTTACACAGAAAGGCCTTGTAAGTTGGCGA5840.14929799265781105No Hit
GTGTAAATGTATATTAGGAAAAGAGGGTGTTTTCCAATGAGGGTTAAAGA5780.14776410917160065No Hit
GGGAAATACAGGTCTCTCACTCTGGGATCTTGCAGAGTTTGGTGGAAGGT5730.146485872933092No Hit
GCCTAGCAGCCATGGAAACGATGTATATTTGCGGGAGAGAACAACAGAGT5700.1457189311899868No Hit
TTGTACACCTCACCATACTGCACTCAGGCAAGCAATTCTTTGCTGGGGGG5660.14469634219917987No Hit
ATATAATACACCCGCCTTCCATAGAGTGTGTAAATAATGTCTGGTTTGGA5570.1423955169698643No Hit
GTCCTGATGTGATGTTCTCCATGTTCAGCGCAGGGTCCCCAATCCTCGAG5510.1408616334836539No Hit
GGATAATACGGTTTAATACCCTTATCCAATGGCAAATATTTGGTAACCTT5400.13804951375893487No Hit
GTATCGGGAAGCCTTAGAGTCTCCTGAGCATTGTACACCTCACCATACTG5360.13702692476812794No Hit
CACACATCGTCAATCTTCTCGAGGATTGGGGACCCTGCGCTGAACATGGA5320.13600433577732102No Hit
GTGTAAACAATACCTGAACCTTTACCCCGTTGCCCGGCAACGGCCAGGTC5250.1342148050434089No Hit
GTCCAGAAGAACCAACAAGAAGATGAGGCATAGCAGCAGGATGAAGAGGA5100.13038009632788292No Hit
CTAGTAAACTGAGCCAGGAGAAACGGGCTGAGGCCCACTCCCATAGGAAT5070.12961315458477773No Hit
GTATTATCCAGAACATCTAGTTAATCATTACTTCCAAACCAGACATTATT5050.1291018600893743No Hit
GTTTAAATGTATACCCAAAGACAAAAGAAAATTGGTAACAGCGGTAAAAA4950.12654538761235698No Hit
GTCCAAGGAATACTAACATTGAGATTCCCGAGATTGAGATCTTCTGCGAC4930.12603409311695352No Hit
GCCTTAGAGTCTCCTGAGCATTGTACACCTCACCATACTGCACTCAGGCA4910.12552279862155002No Hit
GTATTAAACCGTATTATCCAGAACATCTAGTTAATCATTACTTCCAAACC4900.1252671513738483No Hit
AGCTGGAGGAGTGCGAATCCACACTCCGAAAGACACCAAATACTCTATGA4870.12450020963074312No Hit
CAATAGGCCTGTTAACAGGAAGTTTTCTAAAACATTCTTTGATTTTCTGT4870.12450020963074312No Hit
GCACGGGACCATGCAGAACCTGCACGACTCCTGCTCAAGGAACCTCTATG4860.12424456238304138No Hit
GTTATGTTAACACTAATATGGGCCTAAAGTTCAGGCAACTATTGTGGTTT4840.12373326788763792No Hit
ATCCAGAACATCTAGTTAATCATTACTTCCAAACCAGACATTATTTACAC4840.12373326788763792No Hit
CTTTTACACAATGTGGTTATCCTGCTTTAAAGCCTTTGTATGCATGTATT4840.12373326788763792No Hit
GTGTAAATAATGTCTGGTTTGGAAGTAATGATTAACTAGATGTTCTGGAT4830.1234776206399362No Hit
GACGCAAATTGCACCTGTATTCCCATCCCATCATCCTGGGCTTTCGGAAA4810.12296632614453272No Hit
CTATGTATCCCTCCTGTTGCTGTACAAAACCTTCGGACGGAAATTGCACC4790.12245503164912926No Hit
GTATGGATCGGCAGAGGAGCCAGAAAGGTTCCACGCATGCGCTGATGGCC4750.12143244265832233No Hit
ATATAACTGAAAGCCAAACAGTGGGGGAAAGCCCTACGAACCACTGAACA4740.1211767954106206No Hit
TAACATAACTGACTACTAGGTCCCTGGATGTTGGATCTTGCAAATTACCA4730.12092114816291888No Hit
GAATACTAACATTGAGATTCCCGAGATTGAGATCTTCTGCGACGCGGCGA4630.11836467568590156No Hit
GTATTTCCCTGCTGGTGGCTCCAGTTCAGGAACAGTAAACCCTGTTCCGA4630.11836467568590156No Hit
CACCAAGACATTATCAAAAAATGTGAACAATTTGTAGGCCCACTCACAGT4620.11810902843819983No Hit
TTATTTACACACTCTATGGAAGGCGGGTGTATTATATCAGAGAGAAACAA4570.11683079219969117No Hit
TCCTTGGACTCATAAGGTGGGAAACTTTACGGGGCTTTACTCTTCTACTG4540.11606385045658599No Hit
GCTTTAAAGCCTTTGTATGCATGTATTCAATCTAAGCTGGCTTTCACTTT4530.11580820320888426No Hit
GTGCAGAGGTGAAGCGAAGTGCACACGGTCCGGCAGATGAGAAGGCACAG4500.11504126146577906No Hit
AGTCTGTACAGCATCTTGAGGCCCTTTTTACCGCTGTTACCAATTTTCTT4490.11478561421807733No Hit
ATACTGCACTCAGGCAAGCAATTCTTTGCTGGGGGGAACTAATGAATTTA4480.1145299669703756No Hit
CCTGTATTCCCATCCCATCATCCTGGGCTTTCGGAAAATTCCTATGGGAG4450.1137630252272704No Hit
CCTTATGAGTCCAAGGAATACTAACATTGAGATTCCCGAGATTGAGATCT4420.11299608348416522No Hit
GACGGGGAGTCCGCGTAAAGAGAGGTGCGCCCCGTGGTCGGTCGAAACGG4390.11222914174106001No Hit
TTCCCCCACTGTTTGGCTTTCAGTTATATGGATGATGTGGTATTGGGGGC4380.11197349449335828No Hit
GTTTACTAGTGCCATTTGTTCAGTGGTTCGTAGGGCTTTCCCCCACTGTT4370.11171784724565656No Hit
GGCCTATTGATTGGAAAGTCTGTCAACGTATTGTGGGTCTTTTGGGTTTT4350.1112065527502531No Hit
ATGTGGTATTGGGGGCCAAGTCTGTACAGCATCTTGAGGCCCTTTTTACC4290.10967266926404272No Hit
CCTTTACCCCGTTGCCCGGCAACGGCCAGGTCTATGCCAAGTGTTTGCTG4250.10865008027323578No Hit
GGTCTATGCCAAGTGTTTGCTGACGCAACCCCCACTGGCTGGGGCTTGGT4250.10865008027323578No Hit
GTATGCATGTATTCAATCTAAGCTGGCTTTCACTTTCTCGCCAACTTACA4230.10813878577783233No Hit
TCCCGATACAGAGCTGAGGCGGTATCCAGAAGATCTCGTACGTTAGGAAA4220.10788313853013058No Hit
GTGTGAGATACAGTAGTCGGAACAGGGTTTACTGTTCCTGAACTGGAGCC4200.10737184403472712No Hit
GTATTCCCCTGCTGGTGGCTCCAGTTCAGGAACAGTAAACCCTGTTCCGA4170.10660490229162194No Hit
CTTCTGGACTATCAAGGTATGTTGCCCGTTTGTCCTCTAATTCCAGGATC4120.10532666605311328No Hit
CTGTAAGTGGGCCTACAAATTGTTCACATTTTTTGATAATGTCTTGGTGT4120.10532666605311328No Hit
GTTCTGGATAATACGGTTTAATACCCTTATCCAATGGCAAATATTTGGTA4100.1048153715577098No Hit
CTCTGGGATCTTGCAGAGTTTGGTGGAAGGTTGTGGAGTTCCACTGCGTG4060.10379278256690289No Hit
GATCTACAGCATGGGGCAGAATCTTTCCACCAGCAATCCTCTGGGATTCT3950.10098066284218385No Hit
GCATAGACCTGGCCGTTGCCGGGCAACGGGGTAAAGGTTCAGGTATTGTT3940.10072501559448212No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)