FastQCFastQC Report
Fri 2 Sep 2022
EGAF00002490305

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002490305
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences391164
Sequences flagged as poor quality0
Sequence length35-151
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCCATAGAGTGTGTAAATAATGTCTGGTTTGGAAGTAATGATTAACTAGA23210.5933572619157181No Hit
CTCTATGGAAGGCGGGTGTATTATATCAGAGAGAAACAACACATAGCGCC18210.4655336380648526No Hit
GTGCTGGTGCGCAGACCAATTTATGCCTACAGCCTCCTAGTACAAAGATC17470.44661574173492447No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGGAACTACCGTG16180.4136372467814012No Hit
AGTCTAGACTCTGCGGTATTGTGAGGATTCTTGTCAACAAGAAAAACCCC12070.3085662279759896No Hit
CTACAAACCTTGCCAACAAATCCGCCTCCTGCCTCGACCAATCGCCAGTC9770.2497673610045914No Hit
ATTTCCGTCCGAAGGTTTTGTACAGCAACAGGAGGGATACATAGAGGTTC9070.23187205366547023No Hit
GTGTAAAAGGGGCAGCAAAACCCAAAAGACCCACAATACGTTGACAGACT8940.22854863944534776No Hit
GTTTAATACCCTTATCCAATGGCAAATATTTGGTAACCTTTGGATAAAAC8700.2224131055005062No Hit
CATTTACACCAAGACATTATCAAAAAATGTGAACAATTTGTAGGCCCACT8370.21397674632634903No Hit
GGTTTGTAGTATGCCCTGAGCCTGAGGGCTCCACCCCAAAAGGCCTCCGT8260.21116462660163002No Hit
GCCTAATCATCTCTTGTTCATGTCCTACTGTTCAAGCCTCCAAGCTGTGC7670.19608143898722785No Hit
ATATTAGTGTTAACATAACTGACTACTAGGTCCCTGGATGTTGGATCTTG7520.1922467302717019No Hit
GTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATTACAGGGAGTCCAA7510.19199108302400017No Hit
GTACAGACTTGGCCCCCAATACCACATCATCCATATAACTGAAAGCCAAA7450.19045719953778978No Hit
CACTAATATGGGCCTAAAGTTCAGGCAACTATTGTGGTTTCACATTTCTT7430.18994590504238631No Hit
CCCTTATCCAATGGCAAATATTTGGTAACCTTTGGATAAAACCTAGCAGG7430.18994590504238631No Hit
CTCTATGACCGTTTCTCTTCCAAAAGTGAGACAAGAAATGTGAAACCACA7220.18457731284064996No Hit
CCTCCAGCTTATAGACCACCAAATGCCCCTATCTTATCAACACTTCCGGA7200.1840660183452465No Hit
ATGTAAGACCTTGGGCAAGAATTGGTGGGCGTTCACGGTGGTCTCCATGC7170.18329907660214131No Hit
CATTTAAACCCTAACAAAACAAAAAGGTGGGGTTACTCTTTACATTTTAT6900.17639660091419454No Hit
TCTGTGGAGTTACTCTCGTTTTTGCCTTCTGACTTCTTTCCTAACGTACG6900.17639660091419454No Hit
GTATTATATCAGAGAGAAACAACACATAGCGCCTCATTCTGTGGGTCACC6860.17537401192338764No Hit
GGATAAGGGTATTAAACCGTATTATCCAGAACATCTAGTTAATCATTACT6790.1735844811894755No Hit
GTGTAAACAATACCTGAACCTTTACCCCGTTGCCCGGCAACGGCCAGGTC6450.16489247476761665No Hit
GTCATAGAGTATTTGGTGTCTTTCGGAGTGTGGATTCGCACTCCTCCAGC6340.1620803550428976No Hit
TCCTCAGGCCACGCAGTGGAACTCCACAACCTTCCACCAAACTCTGCAAG6180.1579899990796699No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN5980.1528770541256353No Hit
GTAGAAGAGTAAAGCCCCGTAAAGTTTCCCACCTTATGAGTCCAAGGAAT5940.15185446513482836No Hit
CACACATCGTCAATCTTCTCGAGGATTGGGGACCCTGCGCTGAACATGGA5620.14367375320837295No Hit
ACATAGAGGTTCCTTGAGCAGGAGTCGTGCAGGTTCTGCATGGTCCCGTG5610.14341810596067123No Hit
GGATAATACGGTTTAATACCCTTATCCAATGGCAAATATTTGGTAACCTT5600.1431624587129695No Hit
TTGTACACCTCACCATACTGCACTCAGGCAAGCAATTCTTTGCTGGGGGG5600.1431624587129695No Hit
GTGTAAATGTATATTAGGAAAAGAGGGTGTTTTCCAATGAGGGTTAAAGA5560.14213986972216255No Hit
TCACCATACTGCACTCAGGCAAGCAATTCTTTGCTGGGGGGAACTAATGA5500.14060598623595216No Hit
CTTTTACACAATGTGGTTATCCTGCTTTAAAGCCTTTGTATGCATGTATT5500.14060598623595216No Hit
GTATCGGGAAGCCTTAGAGTCTCCTGAGCATTGTACACCTCACCATACTG5480.14009469174054873No Hit
ATACCACATCATCCATATAACTGAAAGCCAAACAGTGGGGGAAAGCCCTA5380.1375382192635314No Hit
CACTCACAGTCAATGAAAAAAGAAGACTGAAATTGATTATGCCTGCTAGG5360.13702692476812794No Hit
GCCTAGCAGCCATGGAAACGATGTATATTTGCGGGAGAGAACAACAGAGT5320.13600433577732102No Hit
TACCTTGATAGTCCAGAAGAACCAACAAGAAGATGAGGCATAGCAGCAGG5310.1357486885296193No Hit
CAATAGGCCTGTTAACAGGAAGTTTTCTAAAACATTCTTTGATTTTCTGT5260.13447045229111063No Hit
GTATGGATCGGCAGAGGAGCCAGAAAGGTTCCACGCATGCGCTGATGGCC5110.13063574357558466No Hit
GTTCATGTCCTACTGTTCAAGCCTCCAAGCTGTGCCTTGGGTGGCTTTAG5080.12986880183247948No Hit
GTCCTGATGTGATGTTCTCCATGTTCAGCGCAGGGTCCCCAATCCTCGAG5080.12986880183247948No Hit
GTATTTCCCTGCTGGTGGCTCCAGTTCAGGAACAGTAAACCCTGTTCCGA5080.12986880183247948No Hit
GTATTAAACCGTATTATCCAGAACATCTAGTTAATCATTACTTCCAAACC5030.1285905655939708No Hit
GGGAAATACAGGTCTCTCACTCTGGGATCTTGCAGAGTTTGGTGGAAGGT5020.1283349183462691No Hit
GTATGCATGTATTCAATCTAAGCTGGCTTTCACTTTCTCGCCAACTTACA4950.12654538761235698No Hit
GCCTTAGAGTCTCCTGAGCATTGTACACCTCACCATACTGCACTCAGGCA4940.12628974036465523No Hit
GTCCAAGAGTCCTCTTATGTAAGACCTTGGGCAAGAATTGGTGGGCGTTC4900.1252671513738483No Hit
ATATAATACACCCGCCTTCCATAGAGTGTGTAAATAATGTCTGGTTTGGA4850.12398891513533965No Hit
GTTTACTAGTGCCATTTGTTCAGTGGTTCGTAGGGCTTTCCCCCACTGTT4830.1234776206399362No Hit
GCACGGGACCATGCAGAACCTGCACGACTCCTGCTCAAGGAACCTCTATG4820.12322197339223447No Hit
GAATACTAACATTGAGATTCCCGAGATTGAGATCTTCTGCGACGCGGCGA4800.12271067889683099No Hit
GTTTAAATGTATACCCAAAGACAAAAGAAAATTGGTAACAGCGGTAAAAA4790.12245503164912926No Hit
GCTTTAAAGCCTTTGTATGCATGTATTCAATCTAAGCTGGCTTTCACTTT4780.12219938440142754No Hit
GTGTAAATAATGTCTGGTTTGGAAGTAATGATTAACTAGATGTTCTGGAT4770.1219437371537258No Hit
GGTCTATGCCAAGTGTTTGCTGACGCAACCCCCACTGGCTGGGGCTTGGT4750.12143244265832233No Hit
AGTCTGTACAGCATCTTGAGGCCCTTTTTACCGCTGTTACCAATTTTCTT4750.12143244265832233No Hit
GTATTATCCAGAACATCTAGTTAATCATTACTTCCAAACCAGACATTATT4730.12092114816291888No Hit
CTATGTATCCCTCCTGTTGCTGTACAAAACCTTCGGACGGAAATTGCACC4720.12066550091521715No Hit
ATGTGGTATTGGGGGCCAAGTCTGTACAGCATCTTGAGGCCCTTTTTACC4680.11964291192441022No Hit
ATATAACTGAAAGCCAAACAGTGGGGGAAAGCCCTACGAACCACTGAACA4580.11708643944739291No Hit
GTATTCCCCTGCTGGTGGCTCCAGTTCAGGAACAGTAAACCCTGTTCCGA4550.11631949770428772No Hit
AGCTGGAGGAGTGCGAATCCACACTCCGAAAGACACCAAATACTCTATGA4540.11606385045658599No Hit
CTGCTAGGCTGTGCTGCCAACTGGATCCTGCGCGGGACGTCCTTTGTTTA4530.11580820320888426No Hit
CTAGTAAACTGAGCCAGGAGAAACGGGCTGAGGCCCACTCCCATAGGAAT4500.11504126146577906No Hit
GACGCAAATTGCACCTGTATTCCCATCCCATCATCCTGGGCTTTCGGAAA4500.11504126146577906No Hit
GTCCAAGGAATACTAACATTGAGATTCCCGAGATTGAGATCTTCTGCGAC4500.11504126146577906No Hit
ATACTGCACTCAGGCAAGCAATTCTTTGCTGGGGGGAACTAATGAATTTA4490.11478561421807733No Hit
GGGTAAAGGTTCAGGTATTGTTTACACAGAAAGGCCTTGTAAGTTGGCGA4490.11478561421807733No Hit
CACCAAGACATTATCAAAAAATGTGAACAATTTGTAGGCCCACTCACAGT4490.11478561421807733No Hit
GTCCAGAAGAACCAACAAGAAGATGAGGCATAGCAGCAGGATGAAGAGGA4470.11427431972267388No Hit
TCCTTGGACTCATAAGGTGGGAAACTTTACGGGGCTTTACTCTTCTACTG4470.11427431972267388No Hit
CCTTTACCCCGTTGCCCGGCAACGGCCAGGTCTATGCCAAGTGTTTGCTG4450.1137630252272704No Hit
GGCCTATTGATTGGAAAGTCTGTCAACGTATTGTGGGTCTTTTGGGTTTT4430.11325173073186694No Hit
ACCCTTCTCGGGGCCGCTTGGGACTCTCTCGTCCCCTTCTCCGTCTGCCG4430.11325173073186694No Hit
TTATTTACACACTCTATGGAAGGCGGGTGTATTATATCAGAGAGAAACAA4420.11299608348416522No Hit
GTGTGAGATACAGTAGTCGGAACAGGGTTTACTGTTCCTGAACTGGAGCC4410.11274043623646349No Hit
CCTTATGAGTCCAAGGAATACTAACATTGAGATTCCCGAGATTGAGATCT4350.1112065527502531No Hit
GTTCAAGCCTCCAAGCTGTGCCTTGGGTGGCTTTAGGACATGGACATTGA4290.10967266926404272No Hit
GTTCTGGATAATACGGTTTAATACCCTTATCCAATGGCAAATATTTGGTA4270.10916137476863924No Hit
GCTTTAGGACATGGACATTGATCCTTATAAAGAATTTGGAGCTTCTGTGG4270.10916137476863924No Hit
ATCCAGAACATCTAGTTAATCATTACTTCCAAACCAGACATTATTTACAC4220.10788313853013058No Hit
AATATACATCGTTTCCATGGCTGCTAGGCTGTGCTGCCAACTGGATCCTG4200.10737184403472712No Hit
TTCCCCCACTGTTTGGCTTTCAGTTATATGGATGATGTGGTATTGGGGGC4160.10634925504392019No Hit
GTCCGGCAGATGAGAAGGCACAGACGGGGAGTCCGCGTAAAGAGAGGTGC4080.10430407706230635No Hit
GTTATGTTAACACTAATATGGGCCTAAAGTTCAGGCAACTATTGTGGTTT4050.10353713531920114No Hit
CTTTAGGACATGGACATTGATCCTTATAAAGAATTTGGAGCTTCTGTGGA4020.10277019357609596No Hit
CTGTAAGTGGGCCTACAAATTGTTCACATTTTTTGATAATGTCTTGGTGT4020.10277019357609596No Hit
CTTCTGGACTATCAAGGTATGTTGCCCGTTTGTCCTCTAATTCCAGGATC4000.10225889908069251No Hit
CCCCTATCTTATCAACACTTCCGGAGACTACTGTTGTTAGACGACGAGGC3970.1014919573375873No Hit
CTCTGGGATCTTGCAGAGTTTGGTGGAAGGTTGTGGAGTTCCACTGCGTG3960.10123631008988557No Hit
CCTGTATTCCCATCCCATCATCCTGGGCTTTCGGAAAATTCCTATGGGAG3950.10098066284218385No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)