FastQCFastQC Report
Sat 3 Sep 2022
EGAF00002490306

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002490306
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences480397
Sequences flagged as poor quality0
Sequence length35-151
%GC48

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTCCAGCCTATAGACCACCAAATGCCCCTATCTTATCAACACTTCCGGA30440.6336425914399966No Hit
GCATAAAACCTCGCAAAGGCATGGGGACGAATCTTTCTGTTCCCAACCCT24930.518945788587356No Hit
GTCTTTGAAGTAGGCCTCAAGGTCGGTCGTTGACATTGCTGGGAGTCCAA24500.5099948584191825No Hit
GCATGGGAGGTTGGTCATCAAAACCTCGCAAAGGCATGGGGACGAATCTT22160.461285145410983No Hit
GTACGGGACCATGCAAAACCTGCACGACTCCTGCTCAAGGCAACTCTATG21740.45254237640951134No Hit
GTTTTATGCTGTAGCTCTTGTTCCCAAGAATATGGTGACCCGCAAAATGA18610.387387931231877No Hit
GACCTTTAACCTAATCTCCTCCCCCAGCTCCTCCCAGTCTTTAAACACAC17260.3592861737271465No Hit
ATGTAAGACCTTGGGCAGGATCTGATGGGCGTTCACGGTGGTCTCCATGC16290.3390945405570809No Hit
CATTAAGATTCCCGAGATTGAGATCTTCTGCGACGCGGCGATTGAGATCT15740.327645676388487No Hit
ATATGATCCTGTGGCAATGTTCCCCAACTTCCAATTATGTAACCCATGAA15440.3214008413874358No Hit
ATTAATAGGTGTCAACAATTTGTGGGCCCTCTCACTGTAAATGAAAAGAG15220.3168212957199983No Hit
GTTTTGATGACCAACCTCCCATGCTGTAGCTCTTGTTCCCAAGAATATGG15060.31349071705277093No Hit
GTCCCGTACTGGTTGTTGTTGATCCTGGAATTAGAGGACAAACGGGCAAC13980.2910093110489866No Hit
GATCATTAACCTAATCTCCTCCCCCAGCTCCTCCCAGTCTTTAAACACAC13820.28767873238175923No Hit
CCTATTAATAATGTCCTCTTGTAAATGAATCTTAGGAAAGGAAGGAGTTT13680.28476447604793537No Hit
GTATACATGCATACAAAGGCATTAAGGCAGGATATCCACATTGGGTAAAT13390.27872780221358584No Hit
GCTCATCGGAACTGACAATTCTGTCGTCCTCTCGCGGAAATATACATCGT12810.26665445454488684No Hit
CTATATAAGAGGGAAACCACACGTAGCGCATCATTTTGCGGGTCACCATA11980.24937707770864515No Hit
CTTATATAGAATACCAGCCTTCCAAAGAGTATGTAAATAATGTCTGGTTT11860.24687914370822464No Hit
AGTCTAGACTCTGCGGTATTGTGAGGATTCTTGTCAACAAGAAAAACCCC11560.24063430870717345No Hit
GTGCTGGTGCGCAGACCAATTTATGCCTACAGCCTCCTAATACAAAGATC10760.22398141537103688No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGGATCACCCGTG10440.21732025803658225No Hit
GTACAGACTTGGCCCCCAATACCACATCATCCATATAGCTGAAAGCCAAA10430.21711209686988056No Hit
GCCTAGCAGCCATGGAAACGATGTATATTTCCGCGAGAGGACGACAGAAT10300.21440600170275834No Hit
GCTCTGTATCGAGAAGCCTTAGAGTCTCCTGAGCATTGCTCACCTCACCA10250.21336519586924982No Hit
CATGTATACAAGCTAAACAGGCTTTCACTTTCTCGCCAACTTACAAGGCC10120.2106591007021276No Hit
GGATCATATTGTACAAAAGATCAAACACTGTTTTAGAAAACTTCCTGTTA9900.20607955503469005No Hit
CTCTTGTACATGTCCCACTCTTCAAGCCTCCAAGCTGTGCCTTGGGTGGC9370.19504701319949957No Hit
CATTTAAACCCTAACAAAACAAAAAGATGGGGTTATTCCCTAAACTTCAT9160.19067562869876373No Hit
ATGCTGTACAGACTTGGCCCCCAATACCACATCATCCATATAGCTGAAAG9030.18796953353164153No Hit
ATCTCAATGTTAGTATTCCTTGGACTCATAAGGTGGGAAACTTTACTGGG8880.18484711603111592No Hit
GTGCAGAGGTGAAGCGAAGTGCACACGGACCGGCAGATGAGAAGGCACAG8870.18463895486441423No Hit
ATATAAGAGGGAAACCACACGTAGCGCATCATTTTGCGGGTCACCATATT8850.18422263253101082No Hit
CCCATAGGTATTTTGCGAAAGCCCAGGACGATGGGATGGGAATACAGGTG8370.17423089652932885No Hit
ACTGTGGAGTTACTCTCGTTTTTGCCTTCTGACTTCTTTCCTTCCGTCAG8320.17319009069582034No Hit
ACCTATGGGAGTGGGCCTCAGTCCGTTTCTCTTGGCTCAGTTTACTAGTG8300.17277376836241692No Hit
GTATATTTCCGCGAGAGGACGACAGAATTGTCAGTTCCGATGAGCTTTGC8240.17152480136220669No Hit
GTACAGCATCGTGAGTCCCTTTATACCGCTGTTACCAATTTTCTTTTGTC8090.16840238386168108No Hit
GGGTAAATGGAGCAGCAAAGCCCAAAAGACCCACAATTCTTTGACATACT7980.1661126110279623No Hit
GGTTAACGAGATGTGAGAGGCAATATTCGGAGCAGGGTTTACTGTTCCTG7840.1631983546941384No Hit
GTATAAAGGGACTCACGATGCTGTACAGACTTGGCCCCCAATACCACATC7790.16215754886062986No Hit
GTCTTTAAACACACAGTCTTTGAAGTAGGCCTCAAGGTCGGTCGTTGACA7740.1611167430271213No Hit
ATGTATATTTCCGCGAGAGGACGACAGAATTGTCAGTTCCGATGAGCTTT7680.15986777602691107No Hit
CTAGTAAACTGAGCCAAGAGAAACGGACTGAGGCCCACTCCCATAGGTAT7590.1579943255265957No Hit
GCCTAATCATCTCTTGTACATGTCCCACTCTTCAAGCCTCCAAGCTGTGC7460.15528823035947353No Hit
GGGTATACATTTAAACCCTAACAAAACAAAAAGATGGGGTTATTCCCTAA7420.1544555856926667No Hit
AGTCTGTACAGCATCGTGAGTCCCTTTATACCGCTGTTACCAATTTTCTT7410.154247424525965No Hit
CAATAGGCCTGTTAACAGGAAGTTTTCTAAAACAGTGTTTGATCTTTTGT7330.15258213519235134No Hit
ATCTTAATGTTAGTATTCCTTGGACTCATAAGGTGGGAAACTTTACTGGG7310.1521658128589479No Hit
CATTGAGATTCCCGAGATTGAGATCTTCTGCGACGCGGCGATTGAGATCT7280.1515413293588428No Hit
CTTGTACATGTCCCACTCTTCAAGCCTCCAAGCTGTGCCTTGGGTGGCTT7270.15133316819214107No Hit
GTGTTTGATCTTTTGTACAATATGATCCTGTGGCAATGTTCCCCAACTTC7170.149251556525124No Hit
CCTTTATACCGCTGTTACCAATTTTCTTTTGTCTCTGGGTATACATTTAA7100.14779442835821205No Hit
CTTTCGGAGTGTGGATTCGCACTCCTCCAGCCTATAGACCACCAAATGCC7050.14675362252470353No Hit
GGAATTAGAGGACAAACGGGCAACATACCTTGATAATCCAGAAGAACCAA7040.1465454613580018No Hit
GTACAAAACCTACGGATGGAAATTGCACCTGTATTCCCATCCCATCGTCC7020.1461291390245984No Hit
ACTCTATGTTTCCCTCATGTTGCTGTACAAAACCTACGGATGGAAATTGC6970.14508833319108988No Hit
TCCAAAGAGTATGTAAATAATGTCTGGTTTGGAAGTAATGATTAACTACC6930.14425568852428305No Hit
GTATGGATCGGCAGAGGAGCCACAAAGGTTCCACGCATGCGCTGATGGCC6910.14383936619087961No Hit
CTGGCTGGGGCTTGGCCATAGGCCATCAGCGCATGCGTGGAACCTTTGTG6900.14363120502417792No Hit
GTACAATATGATCCTGTGGCAATGTTCCCCAACTTCCAATTATGTAACCC6850.14259039919066938No Hit
GTACCTGAACCTTTACCCCGTTGCTCGGCAACGGCCTGGTCTGTGCCAAG6820.14196591569056424No Hit
GGCTGGGGCTTGGCCATAGGCCATCAGCGCATGCGTGGAACCTTTGTGGC6780.14113327102375745No Hit
GTGGATATCCTGCCTTAATGCCTTTGTATGCATGTATACAAGCTAAACAG6750.1405087875236523No Hit
GGATTAAAGATAGGTACTTTAGAGGAATAAAGCCCAGTAAAGTTTCCCAC6620.13780269235653012No Hit
CTCACGATGCTGTACAGACTTGGCCCCCAATACCACATCATCCATATAGC6580.1369700476897233No Hit
CTCTTTACGCGGTCTCCCCGTCTGTGCCTTCTCATCTGCCGGTCCGTGTG6540.13613740302291646No Hit
TCGTCAACCTCCGCGAGGACTGGGGACCCTGTGACGAACATGGAGAACAT6460.1344721136893028No Hit
GGGTAAAGGTTCAGGTACTGTTTACTTAGAAAGGCCTTGTAAGTTGGCGA6430.13384763018919768No Hit
GTGCTGGTGCGCAGACCAATTTATGCCTACAGCCTCCTAATACAAAGACC6350.13218234085558403No Hit
GACCAGGCCGTTGCCGAGCAACGGGGTAAAGGTTCAGGTACTGTTTACTT6260.13030889035526866No Hit
TCCTCAGGCCATGCAGTGGAATTCCACTGCCTTCCACCAAGCTCTGCAGG6160.1282272786882516No Hit
GTCCAAGAGTCCTCTTATGTAAGACCTTGGGCAGGATCTGATGGGCGTTC6060.12614566702123453No Hit
CTCTTATATAGAATACCAGCCTTCCAAAGAGTATGTAAATAATGTCTGGT6050.1259375058545328No Hit
ATATTGTACAAAAGATCAAACACTGTTTTAGAAAACTTCCTGTTAACAGG5930.12343957185411232No Hit
GGATATCCACATTGGGTAAATGGAGCAGCAAAGCCCAAAAGACCCACAAT5920.12323141068741061No Hit
CCATTTACCCAATGTGGATATCCTGCCTTAATGCCTTTGTATGCATGTAT5900.12281508835400722No Hit
CTCTTTGGAAGGCTGGTATTCTATATAAGAGGGAAACCACACGTAGCGCA5860.12198244368720039No Hit
CCTTTACCCCGTTGCTCGGCAACGGCCTGGTCTGTGCCAAGTGTTTGCTG5850.12177428252049868No Hit
TTCTAAAACAGTGTTTGATCTTTTGTACAATATGATCCTGTGGCAATGTT5830.12135796018709526No Hit
GATGTCGGGACCGAGGCAGGTCCCCTAGAAGAAGAACTCCCTCGCCTCGC5820.12114979902039355No Hit
TTATTAATAGGTGTCAACAATTTGTGGGCCCTCTCACTGTAAATGAAAAG5820.12114979902039355No Hit
ACATAGAGTTGCCTTGAGCAGGAGTCGTGCAGGTTTTGCATGGTCCCGTA5820.12114979902039355No Hit
CTCCTAGACACCGCCTCAGCTCTGTATCGAGAAGCCTTAGAGTCTCCTGA5800.12073347668699014No Hit
GGATAATAAGGTTTAATTCCTTTGTCTAATGGCAAATATTTAGTATGGGT5670.11802738151986795No Hit
GTTTTAGAAAACTTCCTGTTAACAGGCCTATTGATTGGAAAGTATGTCAA5610.1167784145196577No Hit
ATACCACATCATCCATATAGCTGAAAGCCAAACAATGGGGGAAAGCCCTA5580.11615393101955258No Hit
GGTTGGGGCTTGGCCATAGGCCATCAGCGCATGCGTGGAACCTTTGTGGC5580.11615393101955258No Hit
GTGGTCAATATGCCCTGAGCCTGAGGGCTCCACCCCAAAATACCGCCGTG5550.11552944751944745No Hit
GTACCGCATCTTGAGTCCCTTTATACCGCTGTTACCAATTTTCTTTTGTC5540.11532128635274574No Hit
TTATTATCCAGATCAGGTAGTTAATCATTACTTCCAAACCAGACATTATT5500.11448864168593892No Hit
GTTTACGTCCCGTCGGCGCTGAATCCCGCGGACGACCCCTCTCGGGGCCG5440.11323967468572868No Hit
GTTTACTAGTGCCATTTGTTCAGTGGTTCGTAGGGCTTTCCCCCATTGTT5430.11303151351902697No Hit
GGGTAGGATAGAATCTAGCAGGCATAATTAATTTCAATCTTCTCTTTTCA5420.11282335235232527No Hit
TGTATACCCAGAGACAAAAGAAAATTGGTAACAGCGGTATAAAGGGACTC5420.11282335235232527No Hit
GTATTTTCCTGCTGGTGGCTCCAGTTCAGGAACAGTAAACCCTGCTCCGA5410.11261519118562356No Hit
GAATTAGAGGACAAACGGGCAACATACCTTGATAATCCAGAAGAACCAAT5380.11199070768551844No Hit
GACGGGGAGACCGCGTAAAGAGAGGTGCGCCCCGTGGTCGGCTGGAACGG5370.11178254651881674No Hit
CTCTTATGTAAGACCTTGGGCAGGATCTGATGGGCGTTCACGGTGGTCTC5340.1111580630187116No Hit
ATTGACCACAGTGTCAACAATTCCTCCTCCTGCCTCCACCAATCGGCAGT5340.1111580630187116No Hit
GTATTAACATAATTGACTACTAGATCCCTGGATGCTGGATCTTCCAAATT5300.11032541835190478No Hit
CATATATCTTGCCTTACTTTTGGAAGAGAGACTGTACTTGAATATTTGGT5160.10741116201808089No Hit
GGGTTTACTGTTCCTGAACTGGAGCCACCAGCAGGAAAATACAGACCCCT5150.10720300085137917No Hit
CCTGTATTCCCATCCCATCGTCCTGGGCTTTCGCAAAATACCTATGGGAG5140.10699483968467746No Hit
GGTTTAAAGATCAGGCAACTATTGTGGTTTCATATATCTTGCCTTACTTT5130.10678667851797576No Hit
GTTTAAATGTATACCCAGAGACAAAAGAAAATTGGTAACAGCGGTATAAA5120.10657851735127405No Hit
GCAGCACAGCCTAGCAGCCATGGAAACGATGTATATTTCCGCGAGAGGAC5120.10657851735127405No Hit
CATGCATACAAAGGCATTAAGGCAGGATATCCACATTGGGTAAATGGAGC5110.10637035618457234No Hit
GAATACAGGTGCAATTTCCATCCGTAGGTTTTGTACAGCAACATGAGGGA5110.10637035618457234No Hit
CTTTGAAGTAGGCCTCAAGGTCGGTCGTTGACATTGCTGGGAGTCCAAGA5100.10616219501787064No Hit
GTACTTGAATATTTGGTCTCTTTCGGAGTGTGGATTCGCACTCCTCCAGC5100.10616219501787064No Hit
GTCTTGGCCAAAATTCGCAGTCCCCAACCTCCAATCACTCACCAACCTCC5070.1055377115177655No Hit
GGATAGAATCTAGCAGGCATAATTAATTTCAATCTTCTCTTTTCATTTAC5000.10408058335085357No Hit
GGTTTTGTACAGCAACATGAGGGAAACATAGAGTTGCCTTGAGCAGGAGT4970.10345609985074845No Hit
GCCTTAATGCCTTTGTATGCATGTATACAAGCTAAACAGGCTTTCACTTT4920.10241529401723991No Hit
ATCATATTCCTCTTCATCCTGCTGCTATGCCTCATCTTCTTATTGGTTCT4920.10241529401723991No Hit
ATTCTATCCTACCCATACTAAATATTTGCCATTAGACAAAGGAATTAAAC4890.10179081051713479No Hit
CAATGGGGGAAAGCCCTACGAACCACTGAACAAATGGCACTAGTAAACTG4880.10158264935043308No Hit
GTCCTGGGCTTTCGCAAAATACCTATGGGAGTGGGCCTCAGTCCGTTTCT4870.10137448818373138No Hit
GTCTTACATAAGAGGACTCTTGGACTCCCAGCAATGTCAACGACCGACCT4870.10137448818373138No Hit
GCTTTGTATCGAGAAGCCTTAGAGTCTCCTGAGCATTGCTCACCTCACCA4860.10116632701702966No Hit
ACACACGGGTGATCCCCCTAGAAAATTGAGAGAAGTCCACCACGAGTCTA4830.10054184351692454No Hit
GTATGCATGTATACAAGCTAAACAGGCTTTCACTTTCTCGCCAACTTACA4820.10033368235022283No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)