FastQCFastQC Report
Sat 3 Sep 2022
EGAF00002490307

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002490307
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences480397
Sequences flagged as poor quality0
Sequence length35-151
%GC48

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTCCAGCCTATAGACCACCAAATGCCCCTATCTTATCAACACTTCCGGA34710.7225274096216255No Hit
GTACGGGACCATGCAAAACCTGCACGACTCCTGCTCAAGGCAACTCTATG24950.5193621109207593No Hit
GCATAAAACCTCGCAAAGGCATGGGGACGAATCTTTCTGTTCCCAACCCT22890.47648091058020764No Hit
GCATGGGAGGTTGGTCATCAAAACCTCGCAAAGGCATGGGGACGAATCTT21060.4383874170737952No Hit
GTCTTTGAAGTAGGCCTCAAGGTCGGTCGTTGACATTGCTGGGAGTCCAA20950.43609764424007647No Hit
GTTTTATGCTGTAGCTCTTGTTCCCAAGAATATGGTGACCCGCAAAATGA19180.39925311773387423No Hit
ATATGATCCTGTGGCAATGTTCCCCAACTTCCAATTATGTAACCCATGAA14810.3082866878852283No Hit
GTTTTGATGACCAACCTCCCATGCTGTAGCTCTTGTTCCCAAGAATATGG14530.30245817521758045No Hit
GTATACATGCATACAAAGGCATTAAGGCAGGATATCCACATTGGGTAAAT14320.29808679071684463No Hit
CATTAAGATTCCCGAGATTGAGATCTTCTGCGACGCGGCGATTGAGATCT14150.2945480508829156No Hit
ATTAATAGGTGTCAACAATTTGTGGGCCCTCTCACTGTAAATGAAAAGAG13660.2843481537145319No Hit
CCTATTAATAATGTCCTCTTGTAAATGAATCTTAGGAAAGGAAGGAGTTT13580.2826828643809183No Hit
GTACAGACTTGGCCCCCAATACCACATCATCCATATAGCTGAAAGCCAAA13210.2749809012129551No Hit
GACCTTTAACCTAATCTCCTCCCCCAGCTCCTCCCAGTCTTTAAACACAC12850.26748709921169367No Hit
ATGTAAGACCTTGGGCAGGATCTGATGGGCGTTCACGGTGGTCTCCATGC12710.26457284287786975No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGGATCACCCGTG12550.26124226421064245No Hit
GTCCCGTACTGGTTGTTGTTGATCCTGGAATTAGAGGACAAACGGGCAAC11910.2479199495417332No Hit
CTTATATAGAATACCAGCCTTCCAAAGAGTATGTAAATAATGTCTGGTTT11690.24334040387429565No Hit
GGTTTTGTACAGCAACATGAGGGAAACATAGAGTTGCCTTGAGCAGGAGT11190.23293234553921027No Hit
GCTCATCGGAACTGACAATTCTGTCGTCCTCTCGCGGAAATATACATCGT11100.2310588950388949No Hit
CTATATAAGAGGGAAACCACACGTAGCGCATCATTTTGCGGGTCACCATA10940.22772831637166757No Hit
CTCTTGTACATGTCCCACTCTTCAAGCCTCCAAGCTGTGCCTTGGGTGGC10660.2218998037040198No Hit
GCTCTGTATCGAGAAGCCTTAGAGTCTCCTGAGCATTGCTCACCTCACCA10620.22106715903721297No Hit
GGATCATATTGTACAAAAGATCAAACACTGTTTTAGAAAACTTCCTGTTA10120.2106591007021276No Hit
AGTCTAGACTCTGCGGTATTGTGAGGATTCTTGTCAACAAGAAAAACCCC9950.2071203608681986No Hit
GATCATTAACCTAATCTCCTCCCCCAGCTCCTCCCAGTCTTTAAACACAC9430.19629598019970984No Hit
GCCTAGCAGCCATGGAAACGATGTATATTTCCGCGAGAGGACGACAGAAT9390.195463335532903No Hit
CATGTATACAAGCTAAACAGGCTTTCACTTTCTCGCCAACTTACAAGGCC9320.19400620736599103No Hit
GTGCTGGTGCGCAGACCAATTTATGCCTACAGCCTCCTAATACAAAGATC9270.1929654015324825No Hit
CTCTTTACGCGGTCTCCCCGTCTGTGCCTTCTCATCTGCCGGTCCGTGTG8970.1867205665314313No Hit
ACCTATGGGAGTGGGCCTCAGTCCGTTTCTCTTGGCTCAGTTTACTAGTG8970.1867205665314313No Hit
ATCTCAATGTTAGTATTCCTTGGACTCATAAGGTGGGAAACTTTACTGGG8840.1840144713643091No Hit
GTACAGCATCGTGAGTCCCTTTATACCGCTGTTACCAATTTTCTTTTGTC8690.1808920538637835No Hit
CATTTAAACCCTAACAAAACAAAAAGATGGGGTTATTCCCTAAACTTCAT8670.18047573153038007No Hit
CCCATAGGTATTTTGCGAAAGCCCAGGACGATGGGATGGGAATACAGGTG8660.18026757036367838No Hit
GTGTTTGATCTTTTGTACAATATGATCCTGTGGCAATGTTCCCCAACTTC8620.17943492569687153No Hit
ATATAAGAGGGAAACCACACGTAGCGCATCATTTTGCGGGTCACCATATT8620.17943492569687153No Hit
ACTGTGGAGTTACTCTCGTTTTTGCCTTCTGACTTCTTTCCTTCCGTCAG8520.1773533140298545No Hit
GGGTAAATGGAGCAGCAAAGCCCAAAAGACCCACAATTCTTTGACATACT8070.16798606152827766No Hit
GTATAAAGGGACTCACGATGCTGTACAGACTTGGCCCCCAATACCACATC8000.1665289333613657No Hit
ATGCTGTACAGACTTGGCCCCCAATACCACATCATCCATATAGCTGAAAG7950.16548812752785716No Hit
GGCTGGGGCTTGGCCATAGGCCATCAGCGCATGCGTGGAACCTTTGTGGC7860.16361467702754182No Hit
GGTTAACGAGATGTGAGAGGCAATATTCGGAGCAGGGTTTACTGTTCCTG7670.15965961486020938No Hit
GGGTAAAGGTTCAGGTACTGTTTACTTAGAAAGGCCTTGTAAGTTGGCGA7620.15861880902670084No Hit
GCCTAATCATCTCTTGTACATGTCCCACTCTTCAAGCCTCCAAGCTGTGC7600.15820248669329742No Hit
GGGTATACATTTAAACCCTAACAAAACAAAAAGATGGGGTTATTCCCTAA7560.1573698420264906No Hit
GTATATTTCCGCGAGAGGACGACAGAATTGTCAGTTCCGATGAGCTTTGC7540.15695351969308718No Hit
ATCTTAATGTTAGTATTCCTTGGACTCATAAGGTGGGAAACTTTACTGGG7520.15653719735968374No Hit
CCTTTATACCGCTGTTACCAATTTTCTTTTGTCTCTGGGTATACATTTAA7390.15383110219256158No Hit
CTTGTACATGTCCCACTCTTCAAGCCTCCAAGCTGTGCCTTGGGTGGCTT7150.1488352341917206No Hit
GTACAATATGATCCTGTGGCAATGTTCCCCAACTTCCAATTATGTAACCC7110.14800258952491377No Hit
TCCTCAGGCCATGCAGTGGAATTCCACTGCCTTCCACCAAGCTCTGCAGG7070.14716994485810694No Hit
GTACCTGAACCTTTACCCCGTTGCTCGGCAACGGCCTGGTCTGTGCCAAG7030.1463373001913001No Hit
GTTTACGTCCCGTCGGCGCTGAATCCCGCGGACGACCCCTCTCGGGGCCG7010.1459209778578967No Hit
TCCAAAGAGTATGTAAATAATGTCTGGTTTGGAAGTAATGATTAACTACC7010.1459209778578967No Hit
CTGGCTGGGGCTTGGCCATAGGCCATCAGCGCATGCGTGGAACCTTTGTG6970.14508833319108988No Hit
GTGCAGAGGTGAAGCGAAGTGCACACGGACCGGCAGATGAGAAGGCACAG6860.1427985603573711No Hit
CTTTCGGAGTGTGGATTCGCACTCCTCCAGCCTATAGACCACCAAATGCC6860.1427985603573711No Hit
AGTCTGTACAGCATCGTGAGTCCCTTTATACCGCTGTTACCAATTTTCTT6780.14113327102375745No Hit
CAATAGGCCTGTTAACAGGAAGTTTTCTAAAACAGTGTTTGATCTTTTGT6770.14092510985705572No Hit
ACTCTATGTTTCCCTCATGTTGCTGTACAAAACCTACGGATGGAAATTGC6700.13946798169014377No Hit
ATGTATATTTCCGCGAGAGGACGACAGAATTGTCAGTTCCGATGAGCTTT6660.13863533702333694No Hit
CTCTTATATAGAATACCAGCCTTCCAAAGAGTATGTAAATAATGTCTGGT6600.1373863700231267No Hit
GTATGGATCGGCAGAGGAGCCACAAAGGTTCCACGCATGCGCTGATGGCC6590.13717820885642498No Hit
GTACAAAACCTACGGATGGAAATTGCACCTGTATTCCCATCCCATCGTCC6540.13613740302291646No Hit
GTCTTACATAAGAGGACTCTTGGACTCCCAGCAATGTCAACGACCGACCT6530.13592924185621477No Hit
GACCAGGCCGTTGCCGAGCAACGGGGTAAAGGTTCAGGTACTGTTTACTT6440.1340557913558994No Hit
GTATTTTCCTGCTGGTGGCTCCAGTTCAGGAACAGTAAACCCTGCTCCGA6440.1340557913558994No Hit
CCTTTACCCCGTTGCTCGGCAACGGCCTGGTCTGTGCCAAGTGTTTGCTG6290.1309333738553738No Hit
GTCTTTAAACACACAGTCTTTGAAGTAGGCCTCAAGGTCGGTCGTTGACA6260.13030889035526866No Hit
GTGGATATCCTGCCTTAATGCCTTTGTATGCATGTATACAAGCTAAACAG6240.12989256802186525No Hit
GGATTAAAGATAGGTACTTTAGAGGAATAAAGCCCAGTAAAGTTTCCCAC6230.12968440685516355No Hit
CTAGTAAACTGAGCCAAGAGAAACGGACTGAGGCCCACTCCCATAGGTAT6210.1292680845217601No Hit
CATTGAGATTCCCGAGATTGAGATCTTCTGCGACGCGGCGATTGAGATCT6170.1284354398549533No Hit
CTCCTAGACACCGCCTCAGCTCTGTATCGAGAAGCCTTAGAGTCTCCTGA5990.12468853885432257No Hit
TCGTCAACCTCCGCGAGGACTGGGGACCCTGTGACGAACATGGAGAACAT5970.12427221652091915No Hit
TTCTAAAACAGTGTTTGATCTTTTGTACAATATGATCCTGTGGCAATGTT5890.12260692718730551No Hit
GGTTGGGGCTTGGCCATAGGCCATCAGCGCATGCGTGGAACCTTTGTGGC5870.1221906048539021No Hit
CTCTTTGGAAGGCTGGTATTCTATATAAGAGGGAAACCACACGTAGCGCA5870.1221906048539021No Hit
GTCTTGGCCAAAATTCGCAGTCCCCAACCTCCAATCACTCACCAACCTCC5840.12156612135379696No Hit
GTTTTAGAAAACTTCCTGTTAACAGGCCTATTGATTGGAAAGTATGTCAA5740.11948450968677989No Hit
GCCTGAGGATGACTGTCTCTTAGAGGTGGAGAGATGGGAGTAGGCTGCCT5710.11886002618667477No Hit
ATATTGTACAAAAGATCAAACACTGTTTTAGAAAACTTCCTGTTAACAGG5660.11781922035316625No Hit
GTGCTGGTGCGCAGACCAATTTATGCCTACAGCCTCCTAATACAAAGACC5640.11740289801976282No Hit
CTCACGATGCTGTACAGACTTGGCCCCCAATACCACATCATCCATATAGC5610.1167784145196577No Hit
CCATTTACCCAATGTGGATATCCTGCCTTAATGCCTTTGTATGCATGTAT5590.11636209218625429No Hit
GTGGTCAATATGCCCTGAGCCTGAGGGCTCCACCCCAAAATACCGCCGTG5580.11615393101955258No Hit
TTATTAATAGGTGTCAACAATTTGTGGGCCCTCTCACTGTAAATGAAAAG5500.11448864168593892No Hit
CTCCGAAAGAGACCAAATATTCAAGTACAGTCTCTCTTCCAAAAGTAAGG5490.11428048051923721No Hit
GACGGGGAGACCGCGTAAAGAGAGGTGCGCCCCGTGGTCGGCTGGAACGG5480.11407231935253549No Hit
CCTGTATTCCCATCCCATCGTCCTGGGCTTTCGCAAAATACCTATGGGAG5470.11386415818583379No Hit
GATGTCGGGACCGAGGCAGGTCCCCTAGAAGAAGAACTCCCTCGCCTCGC5460.11365599701913208No Hit
GTATGTAAATAATGTCTGGTTTGGAAGTAATGATTAACTACCTGATCTGG5430.11303151351902697No Hit
GTTTACTAGTGCCATTTGTTCAGTGGTTCGTAGGGCTTTCCCCCATTGTT5420.11282335235232527No Hit
GTCCAAGAGTCCTCTTATGTAAGACCTTGGGCAGGATCTGATGGGCGTTC5410.11261519118562356No Hit
GTCCTGGGCTTTCGCAAAATACCTATGGGAGTGGGCCTCAGTCCGTTTCT5380.11199070768551844No Hit
GGGTAGGATAGAATCTAGCAGGCATAATTAATTTCAATCTTCTCTTTTCA5370.11178254651881674No Hit
GGAATTAGAGGACAAACGGGCAACATACCTTGATAATCCAGAAGAACCAA5360.11157438535211503No Hit
GTTTAAATGTATACCCAGAGACAAAAGAAAATTGGTAACAGCGGTATAAA5320.11074174068530819No Hit
GTACCGCATCTTGAGTCCCTTTATACCGCTGTTACCAATTTTCTTTTGTC5320.11074174068530819No Hit
GTACTTGAATATTTGGTCTCTTTCGGAGTGTGGATTCGCACTCCTCCAGC5300.11032541835190478No Hit
TTATTATCCAGATCAGGTAGTTAATCATTACTTCCAAACCAGACATTATT5280.10990909601850135No Hit
GGCCTATTGATTGGAAAGTATGTCAAAGAATTGTGGGTCTTTTGGGCTTT5260.10949277368509794No Hit
GCCTTAATGCCTTTGTATGCATGTATACAAGCTAAACAGGCTTTCACTTT5250.10928461251839623No Hit
ACATAGAGTTGCCTTGAGCAGGAGTCGTGCAGGTTTTGCATGGTCCCGTA5240.10907645135169453No Hit
TGTATACCCAGAGACAAAAGAAAATTGGTAACAGCGGTATAAAGGGACTC5240.10907645135169453No Hit
GCTTTGTATCGAGAAGCCTTAGAGTCTCCTGAGCATTGCTCACCTCACCA5230.10886829018499283No Hit
ATCCTACCCATACTAAATATTTGCCATTAGACAAAGGAATTAAACCTTAT5220.10866012901829113No Hit
CCTCTGCACGTTGCATGGAGACCACCGTGAACGCCCATCAGATCCTGCCC5200.10824380668488771No Hit
GGGTTTACTGTTCCTGAACTGGAGCCACCAGCAGGAAAATACAGACCCCT5180.1078274843514843No Hit
GGATATCCACATTGGGTAAATGGAGCAGCAAAGCCCAAAAGACCCACAAT5120.10657851735127405No Hit
CATATATCTTGCCTTACTTTTGGAAGAGAGACTGTACTTGAATATTTGGT4970.10345609985074845No Hit
GTATGCATGTATACAAGCTAAACAGGCTTTCACTTTCTCGCCAACTTACA4930.10262345518394161No Hit
ATTGACCACAGTGTCAACAATTCCTCCTCCTGCCTCCACCAATCGGCAGT4890.10179081051713479No Hit
GGATAATAAGGTTTAATTCCTTTGTCTAATGGCAAATATTTAGTATGGGT4850.10095816585032795No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)