FastQCFastQC Report
Fri 2 Sep 2022
EGAF00002490308

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002490308
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences319383
Sequences flagged as poor quality0
Sequence length35-151
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTCCCGCTTACAGACCACCAAATGCCCCTATCTTATCAACACTTCCGGA21100.6606488134935172No Hit
GTGCTGGTGTACAGACCAATTTATGCCTACAGCCTCCTAGTACCTCCCCC10820.338778206729851No Hit
CCTAGTACAAAGACCTTTAACCTAATCTCCTCCCCCAACTCCTCCCAGTC10090.3159216364051938No Hit
CTATATAAGAGAGAAACTACACGCAGCGCCTCATTTTGTGGGTCACCATA9110.2852374735035991No Hit
CCCTAGCAGCCATGGAAAGGAGGTGTATTTCCGAGAGAGGACAACAGAGT9080.2842981623943666No Hit
GTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATTGCTGAGAGTCCAA8890.2783491920358942No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGGAGCACCCACG8620.2698953920528018No Hit
CTTATATAGAATGCCAGCCTTCCACAGAGTATGTAAATAATGCCTAGTTT8100.25361399949277197No Hit
ATGTAAGACCTTGGGCAAGACCTGGTGGGCGTTCACGGTGGTCTCCATGC7820.2448470958066021No Hit
GTTGTCAATATGCCCTGAGCCTGAGGGCTCCACCCCAAAAGACCGCCGTG7490.23451467360504474No Hit
ATATAAGAGAGAAACTACACGCAGCGCCTCATTTTGTGGGTCACCATATT7430.23263605138657975No Hit
GTGTAAAAGGGGCAGCAAAGCCCAAAAGACCCACAATTCTTTGACATACT7310.2288788069496498No Hit
ATTTACATACTCTGTGGAAGGCTGGCATTCTATATAAGAGAGAAACTACA7300.22856570324657227No Hit
GTATGTAAATAATGCCTAGTTTTGAAGTAATGATTAACTGCATATTCAGG6960.21792017734193742No Hit
AGTCTAGACTCTGTGGTATTGTGAGGATTCTTGTCAACAAGAAAAACCCC6850.21447603660808495No Hit
CCCTTAACTTCATGGGATATGTAATTGGAAGTTGGGGTACTTTACCACAG6620.20727465143730253No Hit
ATTGACAACAGTGCCAGCAGCACCTCCTCCTGCCTCCACCAATCGGCAGT6580.20602223662499258No Hit
CCTCTATTACCAATTTTCTTTTGTCTTTGGGTATACATTTGAATCCTAAT6340.19850774775113264No Hit
GCCTAATCATCTCATGTTCATGTCCTACTGTTCAAGCCTCCAAGCTGTGC6270.19631602182959015No Hit
CTCTTACAGTTAATGAAAAAAGGAGATTAAAATTAATAATGCCTGCTAGG6170.1931849847988152No Hit
GCATATTCAGGATAATACGGTTTAATGCCTTTATCCAAGGGCAAATACTT6170.1931849847988152No Hit
GTATACATGCATATAAAGGCATCAAGGCAGGATAGCCACATTGTGTAAAA6150.19255877739266022No Hit
CTGTAAGAGGGCCCACATATTGTTGACATCTATTAATAATGTCCTCCTGT6020.18848842925265277No Hit
ATCCTAACCTTACCAAGTATTTGCCCTTGGATAAAGGCATTAAACCGTAT5900.18473118481572282No Hit
GTATCGGGAGGCCTTAGAGTCTCCGGAACATTGTTCACCTCACCATACAG5570.17439876261416543No Hit
CATGTATACAAGCTAAGCAGGCTTTCACTTTCTCGCCAACTTACAAGGCC5510.17252014039570046No Hit
CTATTAATAATGTCCTCCTGTAAATGAATGTGAGGAAAGGAGGGAGTTTG5430.1700153107710805No Hit
ATATGTTCCTGTGGTAAAGTACCCCAACTTCCAATTACATATCCCATGAA5300.16594496263107303No Hit
TTATTAATAGATGTCAACAATATGTGGGCCCTCTTACAGTTAATGAAAAA5250.16437944411568556No Hit
GGATTAAAGATAGGTACAGTAGAAGAATAAAGCCCAGTAAAGTTTCCCAC5200.1628139256002981No Hit
GGTTAGGATAGAACCTAGCAGGCATTATTAATTTTAATCTCCTTTTTTCA5140.1609353033818331No Hit
CTCCCATAGGAATCTTGCGAAAGCCCAGGATGATGGGATGGGAATACAAG5050.15811737005413562No Hit
ATACCACATCATCCATATAACTGAAAGCCAAACAGTGGGGGAAAGCCCTA4860.1521683996956632No Hit
CTTTAATCCTGAGTGGCAAACTCCCTCCTTTCCTCACATTCATTTACAGG4850.15185529599258568No Hit
GGATAATACGGTTTAATGCCTTTATCCAAGGGCAAATACTTGGTAAGGTT4740.14841115525873325No Hit
GTGTAAACAATATCTGAACCTTTACCCCGTTGCCCGGCAACGGTCAGGTC4710.14747184414950076No Hit
TCCTCAGGCCATGCAGTGGAACTCCACAACATTCCACCAAGCTCTGCTAG4710.14747184414950076No Hit
TTTACATACTCTGTGGAAGGCTGGCATTCTATATAAGAGAGAAACTACAC4700.14715874044642327No Hit
CATATAAAGGCATCAAGGCAGGATAGCCACATTGTGTAAAAGGGGCAGCA4560.14277528860333832No Hit
TCTGTGGAGTTACTCTCTTTTTTGCCTTCTGACTTCTTTCCTTCTATTCG4550.1424621849002608No Hit
GTATAAAGAATTTGGAGCTTCTGTGGAGTTACTCTCTTTTTTGCCTTCTG4540.1421490811971833No Hit
CCTCTATGTTTCCCTCTTGTTGCTGTACAAAACCTTCGGACGGAAACTGC4540.1421490811971833No Hit
CTAGTAAACTGAGCCAGGAGAAACGGACTGAGGCCCACTCCCATAGGAAT4450.13933114786948583No Hit
GTCCTACTGTTCAAGCCTCCAAGCTGTGCCTTGGGTGGCTTTGGGGCATG4380.13713942194794337No Hit
GCCTTAGAGTCTCCGGAACATTGTTCACCTCACCATACAGCACTCAGGCA4370.13682631824486588No Hit
GCACGGGACCATGCAAGACCTGCACGATTCCTGCTCAAGGAACCTCTATG4340.1358870071356334No Hit
CTATTGTGGTTTCACATTTCCTGTCTTACTTTTGGAAGAGAAACTGTTCT4320.1352607997294784No Hit
GTCCCGTGCTGGTAGTTGATGTTCCTGGAAGTAGAGGACAAACGGGCAAC4310.1349476960264009No Hit
CCTTTACCCCGTTGCCCGGCAACGGTCAGGTCTCTGCCAAGTGTTTGCTG4300.13463459232332342No Hit
ATATAACTGAAAGCCAAACAGTGGGGGAAAGCCCTACGAACCACTGAACA4300.13463459232332342No Hit
CTTTTGGAGTGTGGATTCGCACTCCTCCCGCTTACAGACCACCAAATGCC4280.1340083849171684No Hit
CTCTTATATAGAATGCCAGCCTTCCACAGAGTATGTAAATAATGCCTAGT4240.13275597010485843No Hit
GGGTAAAGGTTCAGATATTGTTTACACAGAAAGGCCTTGTAAGTTGGCGA4240.13275597010485843No Hit
ATTTATGCCTACAGCCTCCTAGTACCTCCCCCAACTCCTCCCAGTCTTTA4190.13119045158947096No Hit
GTCTTTAAACAAACAGTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACA4170.13056424418331597No Hit
CCCATAGGAATCTTGCGAAAGCCCAGGATGATGGGATGGGAATACAAGTG4020.12586768863715353No Hit
GCCTGAGGATGACTGTCTCTTAGAGGTGGAGAGATGGGAGTAGGCTGTCT4000.1252414812309985No Hit
ATATTTTCCTGCTGGTGGCTCCAGTTCCGGAACAGTAAACCCTGTTCCGA4000.1252414812309985No Hit
GTACAGACTTGGCCCCCAATACCACATCATCCATATAACTGAAAGCCAAA3980.12461527382484353No Hit
GCACTCAGGCAAGCTATTCTGTGTTGGGGTGAGTTGATGAATCTGGCCAC3920.12273665160637856No Hit
GTCTTACATAAGAGGACTCTTGGACTCTCAGCAATGTCAACGACCGACCT3910.12242354790330105No Hit
GTAGAGACCCAAACGGCCCCGAGACGGGTCGTCCGCGGGATTCAGCGCCG3900.12211044420022356No Hit
GGACTCGTGCAGGTCTTGCATGGTCCCGTGCTGGTAGTTGATGTTCCTGG3860.12085802938791358No Hit
ATATAGGCCCCTCACTCTGGGATCTAGCAGAGCTTGGTGGAATGTTGTGG3860.12085802938791358No Hit
CTTTTACACAATGTGGCTATCCTGCCTTGATGCCTTTATATGCATGTATA3810.11929251087252608No Hit
CTCTTTACGCGGTCTCCCCGTCTGTGCCTTCTCATCTGCCGGACCGTGTG3810.11929251087252608No Hit
CTTTGGGGCATGGACATTGACCCGTATAAAGAATTTGGAGCTTCTGTGGA3780.11835319976329359No Hit
GGAACATATTGTACAAAAGATCAAGCAATGTTTTCGGAAATTGCCTGTAA3780.11835319976329359No Hit
AGTTAAGGGAGTAGCCCCAACGTTTGGTTTTATTAGGATTCAAATGTATA3780.11835319976329359No Hit
GTCCGGCAGATGAGAAGGCACAGACGGGGAGACCGCGTAAAGAGAGGTGC3770.11804009606021611No Hit
GTGGTATTGTGAGGATTCTTGTCAACAAGAAAAACCCCGCCTGTAACACG3760.11772699235713861No Hit
ATTTATGCCTACAGCCTCCTAGTACAAAGACCTTTAACCTAATCTCCTCC3750.1174138886540611No Hit
ACATAGAGGTTCCTTGAGCAGGAATCGTGCAGGTCTTGCATGGTCCCGTG3740.11710078495098361No Hit
GTCCAAGAGTCCTCTTATGTAAGACCTTGGGCAAGACCTGGTGGGCGTTC3740.11710078495098361No Hit
GCACGATTCCTGCTCAAGGAACCTCTATGTTTCCCTCTTGTTGCTGTACA3730.11678768124790612No Hit
ACCAAGGTATGTTGCCCGTTTGTCCTCTACTTCCAGGAACATCAACTACC3720.11647457754482864No Hit
GTCTACGTCCCGTCGGCGCTGAATCCCGCGGACGACCCGTCTCGGGGCCG3710.11616147384175113No Hit
CTAACCTCTTGTCCTCCAACTTGTCCTGGCTATCGCTGGATGTGTCTGCG3680.11522216273251863No Hit
TCCTTTGTCTACGTCCCGTCGGCGCTGAATCCCGCGGACGACCCGTCTCG3650.11428285162328614No Hit
GTTCAAGCCTCCAAGCTGTGCCTTGGGTGGCTTTGGGGCATGGACATTGA3620.11334354051405367No Hit
ATCTGAACCTTTACCCCGTTGCCCGGCAACGGTCAGGTCTCTGCCAAGTG3570.11177802199866618No Hit
GTTCATGTCCTACTGTTCAAGCCTCCAAGCTGTGCCTTGGGTGGCTTTGG3530.11052560718635619No Hit
GGGTTTACTGTTCCGGAACTGGAGCCACCAGCAGGAAAATATAGGCCCCT3530.11052560718635619No Hit
ATGTGGTATTGGGGGCCAAGTCTGTACAACATCTTGAGTCCCTTTTTACC3490.10927319237404622No Hit
GGATACTAACATTGAGATTCCCGAGATTGAGATCTTCTGCGACGCGGCGA3490.10927319237404622No Hit
CCCCAAAAGACCGCCGTGTGGTGGGGTGAACCCTGGCCCGAATGCTCCCG3460.10833388126481372No Hit
CTCTGGGATCTAGCAGAGCTTGGTGGAATGTTGTGGAGTTCCACTGCATG3440.10770767385865872No Hit
GTACCTATCTTTAATCCTGAGTGGCAAACTCCCTCCTTTCCTCACATTCA3420.10708146645250374No Hit
GTGTTGGGGTGAGTTGATGAATCTGGCCACCTGGGTGGGAAGTAATTTGG3400.10645525904634874No Hit
GTCTATTTACAGGCAATTTCCGAAAACATTGCTTGATCTTTTGTACAATA3380.10582905164019374No Hit
GTACAATATGTTCCTGTGGTAAAGTACCCCAACTTCCAATTACATATCCC3370.10551594793711624No Hit
CCTTTATATGCATGTATACAAGCTAAGCAGGCTTTCACTTTCTCGCCAAC3360.10520284423403876No Hit
CTTGTATTCCCATCCCATCATCCTGGGCTTTCGCAAGATTCCTATGGGAG3360.10520284423403876No Hit
GTGCTGGTGTACAGACCAATTTATAGCCTACAGCCTCCTAGTACAAAGAC3340.10457663682788376No Hit
GTTTACTAGTGCCATTTGTTCAGTGGTTCGTAGGGCTTTCCCCCACTGTT3340.10457663682788376No Hit
GTAAATAGACCTATTGATTGGAAAGTATGTCAAAGAATTGTGGGTCTTTT3320.10395042942172877No Hit
TACCTTGGTAGTCCAGAAGAACCAACAAGAAGATGAGGCATAGCAGCAGG3320.10395042942172877No Hit
GTACAAAACCTTCGGACGGAAACTGCACTTGTATTCCCATCCCATCATCC3320.10395042942172877No Hit
ATACAGCACTCAGGCAAGCTATTCTGTGTTGGGGTGAGTTGATGAATCTG3310.10363732571865127No Hit
GTAGAAGAATAAAGCCCAGTAAAGTTTCCCACCTTGTGAGTCCAAGGGAT3290.10301111831249629No Hit
CTCCAAAAGACACCAAATACTCAAGAACAGTTTCTCTTCCAAAAGTAAGA3280.10269801460941878No Hit
CTGGTGTACAGACCAATTTATGCCTACAGCCTCCTAGTACCTCCCCCAAC3280.10269801460941878No Hit
GTACAGTAGAAGAATAAAGCCCAGTAAAGTTTCCCACCTTGTGAGTCCAA3260.1020718072032638No Hit
CATACTGCGGAACTCCTAGCGGCTTGTTTTGCTCGCAGCAGGTCTGGAGC3250.10175870350018629No Hit
GCTTGTATACATGCATATAAAGGCATCAAGGCAGGATAGCCACATTGTGT3230.10113249609403131No Hit
ATTTATAGCCTACAGCCTCCTAGTACAAAGACCTTTAACCTAATCTCCTC3220.10081939239095382No Hit
GTTAAGGGAGTAGCCCCAACGTTTGGTTTTATTAGGATTCAAATGTATAC3220.10081939239095382No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)