FastQCFastQC Report
Sat 3 Sep 2022
EGAF00002490310

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002490310
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences498491
Sequences flagged as poor quality0
Sequence length35-151
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATTGCCGAAAGTCCAA19760.3963963241061524No Hit
GTGCTGGTGAACAGACCAATTTATGCCTACAGCCTCCTAGTACAAAGATC13870.27823972749758774No Hit
GTTGTGAGTATGCCCTGAGCCTGAGGGCTCCACCCCAACAGGCCCCCATG13050.2617900824688911No Hit
ACTCACAACTGTGCCAGCAGCGCCTCCTCCTGCCTCCACCAATCGGCAGT12780.25637373593505197No Hit
GGATAATAGGGTTTGATTCCTTTATCTAAGGGCAAGTATTTGGTAACATT12710.2549694979447974No Hit
GGCATAGACCAGGCCGTTGCCGAGCAACGGGGTAAAGGTTCAGATACTGG12700.25476889251761814No Hit
ACATGTTCCTGTGGCAAGGAGCCCCAACCCCCAATTACATATCCCATGAA12450.24975375683813753No Hit
AGTCTAGACTCTGTGGTATTGTGAGGATTTTTGTCAACAAGAAAAACCCC11790.23651379864430855No Hit
GTTATTAGACAACGAGGCAGGACCCCTAGAAGAAGAACTCCCTCACCTCG11510.23089684668329016No Hit
CCTCCTGCATATAGACCACCAAATGCCCCTATCTTATCAACACTTCCGGA11240.22548050014945106No Hit
GTAAACACACAGTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATTGC10680.21424659622741432No Hit
GTCCGGTGCTGGTGGTTGATGATCCTGGAATTAGAGGACAAACGGGCAAC9980.20020421632486846No Hit
GTGTAAAAGGGGCAGCGAACCCCAAAAGACCCACAATTCGTTGACATACT9880.1981981620530762No Hit
TCTTTTGTCTTTGGGTATACATTTAAACCCTCACAAAACAAAAAGATGGG9840.1973957403443593No Hit
ATGTAAGACTGTGATCAGGTTCCGGTGGGCGTTCACGGTGGTCTCCATGC9680.19418605350949164No Hit
ATGCAGTAGGAGTGCGAATCCACACTCCAAAAGACACCAAATATTCAAGA9530.19117697210180323No Hit
ATATAAAAGAGAGACAACACGTAGCGCCTCATTTTGCGGGTCACCATATT9490.19037455039308632No Hit
CCTACTGCATATAGACCACCAAATGCCCCTATCTTATCAACACTTCCGGA9470.18997333953872786No Hit
TCTCTTGTTCATGTCCTACTGTTCAAGCCTCCAAGCTGTGCCTTGGGTGG9470.18997333953872786No Hit
CATTTAAACCCTCACAAAACAAAAAGATGGGGATATTCCCTTAATTTCAT9430.18917091783001097No Hit
CATCATATCCATGGCTGCTAGGCTGTGCTGCCAACTGGATCCTGCGCGGG9370.18796728526693562No Hit
TAATCAATTTTAGTCTCCTGGTTTCATTTATTGTTAGGGGTCCCACAAAT9330.1871648635582187No Hit
GACATAAGAAAATTGGTAACAGCGGCATAAAGGGACTCAAGGTGTTGTAC9260.18576062556796413No Hit
GTATACATGCATACAAAGGCATTAAAGCAGGATATCCACATTGTGTAAAA9230.18515880928642645No Hit
GATACGATGTATACTTGCGGGACAGCACGACAGAATTGTCAGTCCCGATA9080.18214972787873804No Hit
CATGTATACAAGCTAAACAGGCTTTTACTTTCTCGCCAACTTACAAGGCC8970.17994306817976652No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGGCACACCCGTG8950.17954185732540806No Hit
CTTTTATATAAGATACCCGCCTTCCAAAGAGTATGTAAATAATGTCTCGT8610.17272127280131436No Hit
GGACCATGTTGTACACAAAATCAAATCATGTTTTAGGAAACTTCCTGTAA8520.17091582395670132No Hit
CCCTTATCTCCACCTCTAAGGGACACTCATCCTCAGGCCATGCAGTGGAA8460.16971219139362595No Hit
GCTCAGAATACTGCCTCTGCCATATCGTCAACCTTCTCGAAGACTGGGGA8410.16870916425772983No Hit
TCTCATGTTCATGTCCTACTGTTCAAGCCTCCAAGCTGTGCCTTGGGTGG8200.16449645028696608No Hit
TCCTCAGGCCATGCAGTGGAACTCCACAACTTTCCATCAAACTCTTCAAG8140.1632928177238907No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN8060.1616879743064569No Hit
CTATTATCCAGAGCATGTAGTTAATCATTACTTCCAGACGAGACATTATT8050.16148736887927767No Hit
TTGCTTACACCTATCAACAATGTCCTCCTGCAAATGAATGTCAGGAAAAG8040.16128676345209844No Hit
CTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATTGCCGAAAGTCCAAGA8040.16128676345209844No Hit
GGAATATGATAAAACGCCGCAGACACATCCAGCGATAACCAGGACAAATT7880.1580770766172308No Hit
GAGACACTGTTCTTGAATATTTGGTGTCTTTTGGAGTGTGGATTCGCACT7740.15526860063672163No Hit
GTCTCTGCCAAGTGTTTGCTGACGCAACCCCCACTGGTTGGGGCTTGGCC7730.1550679952095424No Hit
GGTCTCTGCCAAGTGTTTGCTGACGCAACCCCCACTGGTTGGGGCTTGGC7670.15386436264646705No Hit
TACCTTGATAGTCCAGAAGAACCAACAAGAAGATGAGGCATAGCAGCAGG7610.15266073008339168No Hit
GGTCCTAGGAGTCCTGATGCGATGTTCTCCATGTTCGGTACAGGGTCCCC7590.15225951922903322No Hit
GCTTACACCTATCAACAATGTCCTCCTGCAAATGAATGTCAGGAAAAGAA7580.152058913801854No Hit
ATTCTGAGCAGGGCTCACTGTTCCTGAACTGGAGCCACCAGCAGGAAAGT7550.15145709752031633No Hit
GTATCGGGAGGCCTTAGAGTCTCCGGAACATTGTACACCTCACCATACGG7490.15025346495724096No Hit
TCTTATGTCTTTGGGTATACATTTAAACCCTCACAAAACAAAAAGATGGG7410.14864862153980712No Hit
GGGGCACACCCGTGTGTCTTGGCCAAAATTTGCAGTCCCAAATCTCCAGT7390.14824741068544867No Hit
CCTCTAAGGGACACTCATCCTCAGGCCATGCAGTGGAACTCCACAACTTT7210.1446365129962226No Hit
GCACCGGACCATGCAGAACCTGCACGACTCCTGCTCAAGGAACCTCTATG7140.14323227500596802No Hit
GCATAGACCAGGCCGTTGCCGAGCAACGGGGTAAAGGTTCAGATACTGGT7100.1424298532972511No Hit
GTACAGACTTGGCCCCCAATACCACATCATCCATATAACTGAAAGCCAGA7080.14202864244289265No Hit
TCCAAAGAGTATGTAAATAATGTCTCGTCTGGAAGTAATGATTAACTACA7070.14182803701571342No Hit
ACATAGAGGTTCCTTGAGCAGGAGTCGTGCAGGTTCTGCATGGTCCGGTG7030.14102561530699653No Hit
CCCATAGGTATTTTGCGAAAGCCCAAGATGATGGGATGGGAATACAGGTG7030.14102561530699653No Hit
ATCAAGGTATGTTGCCCGTTTGTCCTCTAATTCCAGGATCATCAACCACC7000.14042379902545885No Hit
ATGTGGATATCCTGCTTTAATGCCTTTGTATGCATGTATACAAGCTAAAC6890.13821713932648733No Hit
AGACTGAGGAGCGGGCGGTAGAGCCCCAAGCGGCCCCGGGAGGGGTCGTC6760.13560926877315738No Hit
GACAAAAGAAAATTGGTAACAGCGGCATAAAGGGACTCAAGGTGTTGTAC6720.1348068470644405No Hit
GTCTTCGAGAAGGTTGACGATATGGCAGAGGCAGTATTCTGAGCAGGGCT6710.13460624163726126No Hit
ATACCACATCATCCATATAACTGAAAGCCAGACAGTGGGGGAAAGCCCTA6600.13239958193828977No Hit
CTTGCGAGACAGCACGACAGAATTGTCAGTCCCGATAAGTTTTGCTCCAG6590.13219897651111054No Hit
GGTCCCGCGCATGCGCTGATGGCCTATGGCCAAGCCCCAACCAGTGGGGG6570.13179776565675208No Hit
CTAGTAAACTGAGCCAAGAGAAACGGACTGATGCCCACTCCCATAGGTAT6530.13099534394803516No Hit
GTTCCTGAACTGGAGCCACCAGCAGGAAAGTACAGAGCCCTGACTCTGGG6520.13079473852085594No Hit
CTCCCATAGGTATTTTGCGAAAGCCCAAGATGATGGGATGGGAATACAGG6200.12437536485112068No Hit
GTGGATATCCTGCTTTAATGCCTTTGTATGCATGTATACAAGCTAAACAG6140.12317173228804533No Hit
CTTTTACACAATGTGGATATCCTGCTTTAATGCCTTTGTATGCATGTATA6050.12136628344343228No Hit
ACCTATGGGAGTGGGCATCAGTCCGTTTCTCTTGGCTCAGTTTACTAGTG6040.12116567801625305No Hit
GGGTCCTAGGAGTCCTGATGCGATGTTCTCCATGTTCGGTACAGGGTCCC6010.12056386173471537No Hit
CTCCACGCGACGTGCAGAGGTGAAGCGAAGTGCACACGGTCCGGCAGATG6000.12036325630753614No Hit
TTCCCCCACTGTCTGGCTTTCAGTTATATGGATGATGTGGTATTGGGGGC5990.12016265088035691No Hit
CTCTTTGGAAGGCGGGTATCTTATATAAAAGAGAGACAACACGTAGCGCC5980.11996204545317771No Hit
CCTCTATGTTTCCCTCATGTTGCTGTACAAAACCTACGGACGGAAACTGC5960.11956083459881925No Hit
GTTTACTAGTGCCATTTGTTCAGTGGTTCGTAGGGCTTTCCCCCACTGTC5960.11956083459881925No Hit
TCTGTGGAGTTACTCTCTTTTTTGCCTTCTGACTTCTTTCCTTCTGTTCG5930.11895901831728156No Hit
CCTATCAACAATGTCCTCCTGCAAATGAATGTCAGGAAAAGAAGGAGTTT5920.11875841289010233No Hit
CTCCTACTGCATATAGACCACCAAATGCCCCTATCTTATCAACACTTCCG5910.1185578074629231No Hit
GAATACTAACATTGAGATTCCCGAGATTGAGATCTTCTGCGACGCGGCGA5880.11795599118138542No Hit
GTCCAGAAGAACCAACAAGAAGATGAGGCATAGCAGCAGGATGCAGAGGA5840.1171535694726685No Hit
GCCTTCCGGACTGCCGATTGGTGGAGGCAGGAGGAGGCGCTGCTGGCACA5820.11675235861831004No Hit
GCATGGCCTGAGGATGAGTGTCCCTTAGAGGTGGAGATAAGGGAGTAGGC5790.11615054233677238No Hit
CCTTATGAGTCCAAGGAATACTAACATTGAGATTCCCGAGATTGAGATCT5700.11434509349215934No Hit
GTGGTATTGTGAGGATTTTTGTCAACAAGAAAAACCCCGCCTGTAACACG5700.11434509349215934No Hit
GGGAATTAGTAGTCAGCTATGTCAATGTTAATATGGGCCTAAAATTCAGA5680.11394388263780088No Hit
GGTGTCCCCCCTAGAAAATTGAGAGAAGTCCACCACGAGTCTAGACTCTG5670.11374327721062165No Hit
GTATGTAAATAATGTCTCGTCTGGAAGTAATGATTAACTACATGCTCTGG5650.11334206635626319No Hit
GCCTAATCATCTCTTGTTCATGTCCTACTGTTCAAGCCTCCAAGCTGTGC5600.11233903922036707No Hit
GTGGGGGAAAGCCCTACGAACCACTGAACAAATGGCACTAGTAAACTGAG5590.11213843379318784No Hit
GAGTCCTCTTATGTAAGACTGTGATCAGGTTCCGGTGGGCGTTCACGGTG5580.11193782836600862No Hit
GAATACAGGTGCAGTTTCCGTCCGTAGGTTTTGTACAGCAACATGAGGGA5490.11013237952139557No Hit
GGATATCCACATTGTGTAAAAGGGGCAGCGAACCCCAAAAGACCCACAAT5410.10852753610396176No Hit
GTATGAACCCTGGCCCGAATGCTCCCACTCCCACCTTGTTGGCGTCCGGC5360.10752450896806562No Hit
GGATTAAAGTTAGGTACCGTAGAAGAATAAAGCCCCGTAAAGTTTCCCAC5310.10652148183216947No Hit
GTTTTGCTCCAGACCGGCTGCGAGCAAAACAAGCGGCTAGGAGTTCCGCA5280.10591966555063181No Hit
TATCCACATTGTGTAAAAGGGGCAGCGAACCCCAAAAGACCCACAATTCG5260.10551845469627336No Hit
GTCCCGCAAGTATACATCGTATCCATGGCTGCTAGGCTGTGCTGCCAACT5250.10531784926909413No Hit
ACCAAATACTTGCCCTTAGATAAAGGAATCAAACCCTATTATCCAGAGCA5160.10351240042448108No Hit
CCACCACTTTCCATCAAACTCTTCAAGATCCCAGAGTCAGGGCTCTGTAC5150.10331179499730185No Hit
CTTCTGGACTATCAAGGTATGTTGCCCGTTTGTCCTCTAATTCCAGGATC5140.10311118957012262No Hit
ACCTAACTTTAATCCTGATTGGCAAACTCCTTCTTTTCCTGACATTCATT5140.10311118957012262No Hit
CCTGTATTCCCATCCCATCATCTTGGGCTTTCGCAAAATACCTATGGGAG5090.1021081624342265No Hit
ATTTACATACTCTTTGGAAGGCGGGTATCTTATATAAAAGAGAGACAACA5080.10190755700704728No Hit
ATTCTGTGTTGGGGTGAGTTGATGAATCTAGCCACCTGGGTGGGAAGTAA5080.10190755700704728No Hit
ATTTATGCCTACAGCCTCCTAGTACAAAGATCATTAACCTAATCTCCTCC5060.10150634615268882No Hit
GTCCAAGGAATACTAACATTGAGATTCCCGAGATTGAGATCTTCTGCGAC5020.1007039244439719No Hit
GGGTAAAGGTTCAGATACTGGTTACGTAAAAAGGCCTTGTAAGTTGGCGA4990.10010210816243423No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)