FastQCFastQC Report
Sat 3 Sep 2022
EGAF00002490311

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002490311
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences498491
Sequences flagged as poor quality0
Sequence length35-151
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATTGCCGAAAGTCCAA16630.33360682539905434No Hit
GTTGTGAGTATGCCCTGAGCCTGAGGGCTCCACCCCAACAGGCCCCCATG13650.2738264080996447No Hit
CCTCCTGCATATAGACCACCAAATGCCCCTATCTTATCAACACTTCCGGA12910.2589816064883819No Hit
GGATAATAGGGTTTGATTCCTTTATCTAAGGGCAAGTATTTGGTAACATT12440.24955315141095827No Hit
CCTACTGCATATAGACCACCAAATGCCCCTATCTTATCAACACTTCCGGA12420.2491519405565998No Hit
CATCATATCCATGGCTGCTAGGCTGTGCTGCCAACTGGATCCTGCGCGGG12060.24193014517814765No Hit
GTGCTGGTGAACAGACCAATTTATGCCTACAGCCTCCTAGTACAAAGATC11890.23851985291610078No Hit
GGCATAGACCAGGCCGTTGCCGAGCAACGGGGTAAAGGTTCAGATACTGG11560.2318998738191863No Hit
ACTCACAACTGTGCCAGCAGCGCCTCCTCCTGCCTCCACCAATCGGCAGT11330.2272859489940641No Hit
ACATGTTCCTGTGGCAAGGAGCCCCAACCCCCAATTACATATCCCATGAA11100.22267202416894186No Hit
AGTCTAGACTCTGTGGTATTGTGAGGATTTTTGTCAACAAGAAAAACCCC10870.21805809934381964No Hit
GTTATTAGACAACGAGGCAGGACCCCTAGAAGAAGAACTCCCTCACCTCG10110.20281208687819838No Hit
GCTCAGAATACTGCCTCTGCCATATCGTCAACCTTCTCGAAGACTGGGGA10090.20241087602383992No Hit
TCTTTTGTCTTTGGGTATACATTTAAACCCTCACAAAACAAAAAGATGGG9890.19839876748025542No Hit
GGACCATGTTGTACACAAAATCAAATCATGTTTTAGGAAACTTCCTGTAA9690.19438665893667087No Hit
CATTTAAACCCTCACAAAACAAAAAGATGGGGATATTCCCTTAATTTCAT9490.19037455039308632No Hit
ATGCAGTAGGAGTGCGAATCCACACTCCAAAAGACACCAAATATTCAAGA9300.18656304727668102No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGGCACACCCGTG9290.1863624418495018No Hit
GTGTAAAAGGGGCAGCGAACCCCAAAAGACCCACAATTCGTTGACATACT9240.18535941471360567No Hit
TCTCTTGTTCATGTCCTACTGTTCAAGCCTCCAAGCTGTGCCTTGGGTGG9130.18315275501463416No Hit
ATATAAAAGAGAGACAACACGTAGCGCCTCATTTTGCGGGTCACCATATT8940.17934125189822883No Hit
GTCTCTGCCAAGTGTTTGCTGACGCAACCCCCACTGGTTGGGGCTTGGCC8540.17131703481105978No Hit
GTATACATGCATACAAAGGCATTAAAGCAGGATATCCACATTGTGTAAAA8440.1693109805392675No Hit
GTCCGGTGCTGGTGGTTGATGATCCTGGAATTAGAGGACAAACGGGCAAC8430.16911037511208826No Hit
TAATCAATTTTAGTCTCCTGGTTTCATTTATTGTTAGGGGTCCCACAAAT8390.16830795340337137No Hit
CATGTATACAAGCTAAACAGGCTTTTACTTTCTCGCCAACTTACAAGGCC8330.167104320840296No Hit
CTATTATCCAGAGCATGTAGTTAATCATTACTTCCAGACGAGACATTATT8320.16690371541311677No Hit
GATACGATGTATACTTGCGGGACAGCACGACAGAATTGTCAGTCCCGATA8310.16670310998593757No Hit
CTTTTATATAAGATACCCGCCTTCCAAAGAGTATGTAAATAATGTCTCGT8280.16610129370439988No Hit
GTAAACACACAGTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATTGC8260.16570008285004142No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN8040.16128676345209844No Hit
TCTCATGTTCATGTCCTACTGTTCAAGCCTCCAAGCTGTGCCTTGGGTGG8010.16068494717056075No Hit
CCCTTATCTCCACCTCTAAGGGACACTCATCCTCAGGCCATGCAGTGGAA7920.1588794983259477No Hit
GACATAAGAAAATTGGTAACAGCGGCATAAAGGGACTCAAGGTGTTGTAC7920.1588794983259477No Hit
TCCTCAGGCCATGCAGTGGAACTCCACAACTTTCCATCAAACTCTTCAAG7920.1588794983259477No Hit
GGGGCACACCCGTGTGTCTTGGCCAAAATTTGCAGTCCCAAATCTCCAGT7840.1572746549085139No Hit
GGTCTCTGCCAAGTGTTTGCTGACGCAACCCCCACTGGTTGGGGCTTGGC7710.15466678435518397No Hit
TCTTATGTCTTTGGGTATACATTTAAACCCTCACAAAACAAAAAGATGGG7560.15165770294749553No Hit
GGAATATGATAAAACGCCGCAGACACATCCAGCGATAACCAGGACAAATT7490.15025346495724096No Hit
GTATCGGGAGGCCTTAGAGTCTCCGGAACATTGTACACCTCACCATACGG7450.14945104324852404No Hit
GAGACACTGTTCTTGAATATTTGGTGTCTTTTGGAGTGTGGATTCGCACT7360.147645594403911No Hit
TTGCTTACACCTATCAACAATGTCCTCCTGCAAATGAATGTCAGGAAAAG7120.14283106415160957No Hit
CCTCTAAGGGACACTCATCCTCAGGCCATGCAGTGGAACTCCACAACTTT7080.14202864244289265No Hit
ATGTAAGACTGTGATCAGGTTCCGGTGGGCGTTCACGGTGGTCTCCATGC7070.14182803701571342No Hit
GCTTACACCTATCAACAATGTCCTCCTGCAAATGAATGTCAGGAAAAGAA7070.14182803701571342No Hit
GTGGATATCCTGCTTTAATGCCTTTGTATGCATGTATACAAGCTAAACAG7010.14062440445263807No Hit
GCACCGGACCATGCAGAACCTGCACGACTCCTGCTCAAGGAACCTCTATG6930.13901956103520424No Hit
ATTCTGAGCAGGGCTCACTGTTCCTGAACTGGAGCCACCAGCAGGAAAGT6900.13841774475366656No Hit
ACATAGAGGTTCCTTGAGCAGGAGTCGTGCAGGTTCTGCATGGTCCGGTG6890.13821713932648733No Hit
CTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATTGCCGAAAGTCCAAGA6870.13781592847212887No Hit
AGACTGAGGAGCGGGCGGTAGAGCCCCAAGCGGCCCCGGGAGGGGTCGTC6800.1364116904818743No Hit
GCCTTCCGGACTGCCGATTGGTGGAGGCAGGAGGAGGCGCTGCTGGCACA6770.1358098742003366No Hit
TCCAAAGAGTATGTAAATAATGTCTCGTCTGGAAGTAATGATTAACTACA6740.13520805791879892No Hit
GTACAGACTTGGCCCCCAATACCACATCATCCATATAACTGAAAGCCAGA6730.13500745249161972No Hit
GGTCCTAGGAGTCCTGATGCGATGTTCTCCATGTTCGGTACAGGGTCCCC6580.1319983710839313No Hit
GTCCCGCAAGTATACATCGTATCCATGGCTGCTAGGCTGTGCTGCCAACT6550.13139655480239362No Hit
ATCAAGGTATGTTGCCCGTTTGTCCTCTAATTCCAGGATCATCAACCACC6530.13099534394803516No Hit
CCCATAGGTATTTTGCGAAAGCCCAAGATGATGGGATGGGAATACAGGTG6500.1303935276664975No Hit
TACCTTGATAGTCCAGAAGAACCAACAAGAAGATGAGGCATAGCAGCAGG6490.13019292223931828No Hit
GTCTTCGAGAAGGTTGACGATATGGCAGAGGCAGTATTCTGAGCAGGGCT6380.12798626254034676No Hit
CTCCTACTGCATATAGACCACCAAATGCCCCTATCTTATCAACACTTCCG6360.1275850516859883No Hit
CTTTTACACAATGTGGATATCCTGCTTTAATGCCTTTGTATGCATGTATA6300.12638141912291295No Hit
ATGTGGATATCCTGCTTTAATGCCTTTGTATGCATGTATACAAGCTAAAC6290.12618081369573372No Hit
GCATGGCCTGAGGATGAGTGTCCCTTAGAGGTGGAGATAAGGGAGTAGGC6260.12557899741419604No Hit
CCTCTATGTTTCCCTCATGTTGCTGTACAAAACCTACGGACGGAAACTGC6160.12357294314240379No Hit
GTTCCTGAACTGGAGCCACCAGCAGGAAAGTACAGAGCCCTGACTCTGGG6150.12337233771522456No Hit
GGGAATTAGTAGTCAGCTATGTCAATGTTAATATGGGCCTAAAATTCAGA6110.12256991600650764No Hit
GCATAGACCAGGCCGTTGCCGAGCAACGGGGTAAAGGTTCAGATACTGGT6090.12216870515214918No Hit
ATACCACATCATCCATATAACTGAAAGCCAGACAGTGGGGGAAAGCCCTA6010.12056386173471537No Hit
CCTTATGAGTCCAAGGAATACTAACATTGAGATTCCCGAGATTGAGATCT5940.11915962374446079No Hit
GACAAAAGAAAATTGGTAACAGCGGCATAAAGGGACTCAAGGTGTTGTAC5920.11875841289010233No Hit
GTATGTAAATAATGTCTCGTCTGGAAGTAATGATTAACTACATGCTCTGG5920.11875841289010233No Hit
GTTTACTAGTGCCATTTGTTCAGTGGTTCGTAGGGCTTTCCCCCACTGTC5840.1171535694726685No Hit
GCCTAATCATCTCTTGTTCATGTCCTACTGTTCAAGCCTCCAAGCTGTGC5820.11675235861831004No Hit
CATCGTATCCATGGCTGCTAGGCTGTGCTGCCAACTGGATCCTGCGCGGG5810.11655175319113084No Hit
ACCTATGGGAGTGGGCATCAGTCCGTTTCTCTTGGCTCAGTTTACTAGTG5760.1155487260552347No Hit
TCTGTGGAGTTACTCTCTTTTTTGCCTTCTGACTTCTTTCCTTCTGTTCG5690.1141444880649801No Hit
CTCTTTGGAAGGCGGGTATCTTATATAAAAGAGAGACAACACGTAGCGCC5670.11374327721062165No Hit
CTTGCGAGACAGCACGACAGAATTGTCAGTCCCGATAAGTTTTGCTCCAG5670.11374327721062165No Hit
GGTCCCGCGCATGCGCTGATGGCCTATGGCCAAGCCCCAACCAGTGGGGG5670.11374327721062165No Hit
CCTCCGTCTGCCGTACCGACCGACCACGGGGCGCACCTCTCTTTACGCGG5600.11233903922036707No Hit
TTCCCCCACTGTCTGGCTTTCAGTTATATGGATGATGTGGTATTGGGGGC5600.11233903922036707No Hit
GTATGAACCCTGGCCCGAATGCTCCCACTCCCACCTTGTTGGCGTCCGGC5600.11233903922036707No Hit
CCTATCAACAATGTCCTCCTGCAAATGAATGTCAGGAAAAGAAGGAGTTT5550.11133601208447093No Hit
GAATACTGCCTCTGCCATATCGTCAACCTTCTCGAAGACTGGGGACCCTG5510.11053359037575403No Hit
GAATACTAACATTGAGATTCCCGAGATTGAGATCTTCTGCGACGCGGCGA5470.10973116866703711No Hit
GGTGTAAGCAATTTGTGGGACCCCTAACAATAAATGAAACCAGGAGACTA5440.10912935238549945No Hit
GGATATCCACATTGTGTAAAAGGGGCAGCGAACCCCAAAAGACCCACAAT5440.10912935238549945No Hit
CCTGTAAACAGGCCTATTGATTGGAAAGTATGTCAACGAATTGTGGGTCT5390.1081263252496033No Hit
CTAGTAAACTGAGCCAAGAGAAACGGACTGATGCCCACTCCCATAGGTAT5370.10772511439524485No Hit
CTCCCATAGGTATTTTGCGAAAGCCCAAGATGATGGGATGGGAATACAGG5360.10752450896806562No Hit
CAATACCACAGAGTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGG5300.10632087640499024No Hit
GGATTAAAGTTAGGTACCGTAGAAGAATAAAGCCCCGTAAAGTTTCCCAC5280.10591966555063181No Hit
GAATAAAGCCCCGTAAAGTTTCCCACCTTATGAGTCCAAGGAATACTAAC5280.10591966555063181No Hit
CCACCACTTTCCATCAAACTCTTCAAGATCCCAGAGTCAGGGCTCTGTAC5280.10591966555063181No Hit
GTATTTGGTAACATTGGGATAAAATCTAGCGGGCATAATCAATTTTAGTC5150.10331179499730185No Hit
GTATGCATGTATACAAGCTAAACAGGCTTTTACTTTCTCGCCAACTTACA5150.10331179499730185No Hit
ACCTAACTTTAATCCTGATTGGCAAACTCCTTCTTTTCCTGACATTCATT5150.10331179499730185No Hit
CAAATCTTTCTGTCCCCAATCCACTGGGATTCTTCCCCGATCATCAGTTG5110.10250937328858496No Hit
GCCTAGTCATCTCTTGTTCATGTCCTACTGTTCAAGCCTCCAAGCTGTGC5100.10230876786140573No Hit
TCCTTGGACTCATAAGGTGGGAAACTTTACGGGGCTTTATTCTTCTACGG5030.10090452987115113No Hit
GTCTAATAACAGTAGTCTCCGGAAGTGTTGATAAGATAGGGGCATTTGGT5010.10050331901679267No Hit
GAATACAGGTGCAGTTTCCGTCCGTAGGTTTTGTACAGCAACATGAGGGA5010.10050331901679267No Hit
GTCCAAGGAATACTAACATTGAGATTCCCGAGATTGAGATCTTCTGCGAC4990.10010210816243423No Hit
TATCCACATTGTGTAAAAGGGGCAGCGAACCCCAAAAGACCCACAATTCG4990.10010210816243423No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)