FastQCFastQC Report
Sat 3 Sep 2022
EGAF00002490313

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002490313
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences546666
Sequences flagged as poor quality0
Sequence length35-151
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGCTGGTGCGCAGACCAATTTATGCCTACAGCCTCCTAATACATAAACC24060.44012248795425357No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGGAACTACCGTG22610.4135980653634943No Hit
TCCATAGAGTGTGTAAATAATGTCTGGTTTGGAAGTAATGATTAACTACA22130.4048175668506913No Hit
CTCTATGGAAGGCGGGTGTATTATATAAGAGAGAAACAACACATAGCGCC18860.3450004207322204No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN17580.3215857580314122No Hit
CCTCCAGCTTATAGACCACCAAATGCCCCTATCTTATCAACACTTCCGGA15130.27676863020564657No Hit
ATATAAGACCTTGGGCAAGAATTGGTGGGCGTTCACGGTGGTCTCCATGC14510.2654271529599427No Hit
AGTCTAGACTCTGCGGTATTGTGAGGATTCTTGTCAACAAGAAAAACCCC14010.25628080034243944No Hit
ATCAAGGTATGTTGCCCGTTTGTCCTCTAATTCCAGGATCTTCAACCACC12080.22097587923887713No Hit
TCCTCAGGCCATGCAATGGAACTCCACAACCTTCCACCAAACTCTGCAAG11310.20689049620792221No Hit
CTACAAACCTTGCCAGCAAATCCGCCTCCTGCCTCTACCAATCGACAGTC10970.20067097642802006No Hit
ATATAAGAGAGAAACAACACATAGCGCCTCATTTTGTGGGTCACCATATT10600.19390267549106766No Hit
CCCTTATCCAATGGCAAATATTTGGTAACCTTTGGATAAAACCTAGCAGG9890.18091485477421315No Hit
GCTTTAATGCCCTTGTATGCATGTATTCAATCTAAGCAGGCTTTCACTTT9650.1765246055178116No Hit
TCTGTGGAGTTACTCTCGTTTTTGCCTTCTGACTTCTTTCCTTCAGTACG9390.17176850215670994No Hit
TACCTTGATAGTCCAGAAGAACCAACAAGAAGATGAGGCATAGCAGCAGG8780.16060995196335606No Hit
GTCCCGTGCTGGTGGTTGAAGATCCTGGAATTAGAGGACAAACGGGCAAC8670.15859775438750534No Hit
GGATAAGGGTATTAAACCTTATTATCCAGAACATGTAGTTAATCATTACT8250.15091481818880267No Hit
CTCTATGACCGTTTCTCTTCCAAAAGTGAGACAAGAAATGTGAAACCACA8120.14853676650825184No Hit
ATATTAGTGTTAACATAACTGACTACTAGGTCTCTGGATGTTGGATCTTC8060.14743920419415146No Hit
GCCTAATCATCTCTTGTTCATGTCCTACTGTTCAAGCCTCCAAGCTGTGC7890.14432944430420036No Hit
GGTTTGTAGTATGCCCTGAGCCTGAGGGCTCCACCCCAAAAGGCCTCCGT7800.1426831008330498No Hit
GTCTTATATAAGAGGACTCTTGGACTCTCTGCAATGTCAACGACCGACCT7520.137561143367248No Hit
ATACCACATCATCCATATAACTGAAAGCCAAACAGTGGGGGAAAGCCCTA7510.13737821631489794No Hit
TCCTTAGAGTCTCCTGAGCATTGTTCACCTCACCATACTGCACTCAGGCA7480.13682943515784776No Hit
GTATTATATAAGAGAGAAACAACACATAGCGCCTCATTTTGTGGGTCACC7370.13481723758199704No Hit
ACATAGAGGTTCCTTGAGCAGGAGTCGTGCAGGTTCTGCATGGTCCCGTG7370.13481723758199704No Hit
GCACGGGACCATGCAGAACCTGCACGACTCCTGCTCAAGGAACCTCTATG7350.1344513834772969No Hit
GTCCAAGAGTCCTCTTATATAAGACCTTGGGCAAGAATTGGTGGGCGTTC7340.13426845642494686No Hit
CATTTACACCAAGACATTATTAAAAAATGTGAACAATTTGTAGGCCCACT7310.13371967526789666No Hit
CTCTTATATAATACACCCGCCTTCCATAGAGTGTGTAAATAATGTCTGGT7220.1320733317967461No Hit
GAAACGGGCTGAGGCCCACTCCCATAGGAATTTTCCGAAAGCCCAAGATG7130.13042698832559552No Hit
GTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATTGCAGAGAGTCCAA7100.1298782071685453No Hit
GGGTAAAGGTTCAGGTATTGTTTACACAGAAAGGCCTTGTAAGTTGGCGA7090.12969528011619527No Hit
ATGTGGTATTGGGGGCCAAGTCTGTATCGCATCTTGAGTCCCTTTTTACC7030.1285977178020949No Hit
GCCTGAGGATGAGTGTCTCTTAAAGGTGGAGACAGAGGGGTAGGCTGCCT6920.12658552022624417No Hit
GTGTAAATGTATATTAGGAAAAGAGGGTGTTTTCCAATGAGGGTTAAAGA6880.12585381201684392No Hit
GTTCAGAAACAGTAAACCCTGTTCCGACTACTGTCTCTCACATATCGTCA6780.1240245414933433No Hit
CTATGTATCCCTCCTGTTGCTGTACAAAACCTTCGGACGGAAATTGCACC6780.1240245414933433No Hit
GTGTAAACAATACCTGAACCTTTACCCCGTTGCCCGGCAACGGCCAGGTC6780.1240245414933433No Hit
CATTTAAACCCTAACAAAACAAAAAGATGGGGTTACTCTTTACATTTCAT6780.1240245414933433No Hit
ATATAATACACCCGCCTTCCATAGAGTGTGTAAATAATGTCTGGTTTGGA6610.1209147816033922No Hit
GCCTAGCAGCCATGGAAACGATGTATATTTGCGGGAGAGAACAACAGAGT6560.12000014634164187No Hit
TCACCCCACCGCACGGAGGCCTTTTGGGGTGGAGCCCTCAGGCTCAGGGC6520.11926843813224162No Hit
GAATACTAACAGTGAGATTCCCGAGATTGAGATCTTCTGCGACGCGGCGA6500.1189025840275415No Hit
CACTAATATGGGCCTAAAGTTCAGACAACTATTGTGGTTTCACATTTCTT6490.11871965697519145No Hit
GTCATAGAGTATTTGGTGTCTTTCGGAGTGTGGATTCGCACTCCTCCAGC6490.11871965697519145No Hit
GTGTAAAAGGGGCAGCAAAACCCAAAAGACCCACAATACGTTGACAGACT6360.11634160529464059No Hit
TCCAACGATAACCAGGACAAGTTGGAGGACAGGAGGTTGGTGAGTGATTG6340.11597575118994048No Hit
GGGTATACATTTAAACCCTAACAAAACAAAAAGATGGGGTTACTCTTTAC6330.11579282413759041No Hit
ATGTCATGGGTCATTGCCACAAGATCACATCATACAGAAAATCAAAGAAT6290.11506111592819017No Hit
TAGGAATCCTGATGTGATGTTTTCCATGTTCAGCGCAGGGTCCCCAATCC6280.11487818887584009No Hit
TTATCAAAAAATGTGAACAATTTGTAGGCCCACTCACAGTCAATGAGAAA6230.11396355361408977No Hit
GTATGGATCGGCAGAGGAGCCTGAAAGGTTCCACGCATGCGCTGATGGCC6140.1123172101429392No Hit
CCCATAACATCCAATGACATAGCCCATGAAATGTAAAGAGTAACCCCATC6070.11103672077648874No Hit
TTCCCCCACTGTTTGGCTTTCAGTTATATGGATGATGTGGTATTGGGGGC6030.1103050125670885No Hit
CAATAGGCCTGTTAACAGGAAGTTTTCTAAAACATTCTTTGATTTTCTGT6030.1103050125670885No Hit
CCTCCTGTCCTCCAACTTGTCCTGGTTATCGCTGGATGTGTCTGCGGCGT5950.10884159614828798No Hit
GTCCAGAAGAACCAACAAGAAGATGAGGCATAGCAGCAGGATGAAGAGGA5930.10847574204358786No Hit
CTGCTAGGCTGTGCTGCCAACTGGATCCTGCGCGGGACGTCCTTTGTTTA5870.10737817972948746No Hit
TTTTAATAATGTCTTGGTGTAAATGTATATTAGGAAAAGAGGGTGTTTTC5860.1071952526771374No Hit
ATCGCTGGATGTGTCTGCGGCGTTTTATCATCTTCCTCTTCATCCTGCTG5850.10701232562478735No Hit
GTTTAATACCCTTATCCAATGGCAAATATTTGGTAACCTTTGGATAAAAC5830.10664647152008722No Hit
ACATCGATCCTTATAAAGAATTTGGAGCTTCTGTGGAGTTACTCTCGTTT5800.10609769036303704No Hit
CCTTTACCCCGTTGCCCGGCAACGGCCAGGTCTGTGCCAAGTGTTTGCTG5790.10591476331068697No Hit
CCTCTATGTATCCCTCCTGTTGCTGTACAAAACCTTCGGACGGAAATTGC5690.10408549278718632No Hit
GTCCAAGGAATACTAACAGTGAGATTCCCGAGATTGAGATCTTCTGCGAC5670.10371963868248621No Hit
CAGTAAACCCTGTTCCGACTACTGTCTCTCACATATCGTCAATCTTCTCG5610.10262207636838581No Hit
CTATCAAGGTATGTTGCCCGTTTGTCCTCTAATTCCAGGATCTTCAACCA5610.10262207636838581No Hit
GTTTACTAGTGCCATTTGTTCAGTGGTTCGTAGGGCTTTCCCCCACTGTT5600.10243914931603576No Hit
GCTTTAGGGCATGGACATTGATCCTTATAAAGAATTTGGAGCTTCTGTGG5590.10225622226368569No Hit
GTCATAGGCCATCAGCGCATGCGTGGAACCTTTCAGGCTCCTCTGCCGAT5580.10207329521133561No Hit
ATGTATCCCTCCTGTTGCTGTACAAAACCTTCGGACGGAAATTGCACCTG5490.10042695174018504No Hit
TGTATACCCAGAGACAAAAGAAAATTGGTAACAGCGGTAAAAAGGGACTC5490.10042695174018504No Hit
GTATTAAACCTTATTATCCAGAACATGTAGTTAATCATTACTTCCAAACC5480.10024402468783498No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)