FastQCFastQC Report
Sun 4 Sep 2022
EGAF00002490315

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002490315
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences643381
Sequences flagged as poor quality0
Sequence length35-151
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCCATAGAGTGTGTAAATAATGTCTGGTTTGGAAGTAATGATTAACTAGA63500.9869735040357113No Hit
GTGCTGGTGCGCAGACCAATTTATGCCTACAGCCTCCTAGTACAAAGATC26430.4107985781364386No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN25010.38872767458162427No Hit
CTCTATGGAAGGCGGGTATATTATATAAGAGAGAAACTACACATAGCGCC21170.32904297764466156No Hit
AGTCTAGACTCTGCGGTATTGTGAGGATTCTTGTCAACAAGAAAAACCCC21030.3268669730688348No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGGAACTACCGTG20090.3122566566311408No Hit
GGATAAGGGTATTAAACCTTATTATCCAGAACATCTAGTTAATCATTACT19850.30852636307258063No Hit
CATTTACACCAAGACATTATCAAAAAATGTGAACAATTTGTAGGCCCACT19680.3058840718019339No Hit
CCCTTATCCAATGGCAAATATTTGGTAACATTTGGATAAAACCTAGCAGG16550.2572348266423783No Hit
CTCTATGACCGTTTCTCTTCCAAAAGTGAGACAAGAAATGTGAAACCACA16420.25521425096482486No Hit
CTTATATAATATACCCGCCTTCCATAGAGTGTGTAAATAATGTCTGGTTT14470.22490561580152352No Hit
GTGTAAATAATGTCTGGTTTGGAAGTAATGATTAACTAGATGTTCTGGAT14440.2244393291067035No Hit
CTCTTATATAATATACCCGCCTTCCATAGAGTGTGTAAATAATGTCTGGT14350.22304046902224345No Hit
CTCCAAAAGACACCAAATACTCTATGACCGTTTCTCTTCCAAAAGTGAGA13690.21278216173620298No Hit
GTCTATAAGCTGGAGGAGTGCGAATCCACACTCCAAAAGACACCAAATAC13380.20796386588972943No Hit
TAATAACTCTATCCACCTGGGTGGGTGGTAATTTGGAAGATCCAACATCC13140.20423357233116923No Hit
CATTTAAACCCTAACAAAACAAAAAGATGGGGTTACTCTTTACATTTTAT13040.20267928334843582No Hit
GTTTAATACCCTTATCCAATGGCAAATATTTGGTAACATTTGGATAAAAC12850.19972613428124236No Hit
GTGTAAAAGGGGCAGCAAATCCCAAAAGACCCACAATACGTTGACAGACT12700.19739470080714228No Hit
GTATTAAACCTTATTATCCAGAACATCTAGTTAATCATTACTTCCAAACC12650.19661755631577557No Hit
ATATAATATACCCGCCTTCCATAGAGTGTGTAAATAATGTCTGGTTTGGA12150.18884611140210855No Hit
CACTAATATGGGCCTAAAGTTCAGGCAACTATTGTGGTTTCACATTTCTT12080.1877581091141952No Hit
GGGTATACATTTAAACCCTAACAAAACAAAAAGATGGGGTTACTCTTTAC11440.17781065962470138No Hit
ATGTGGTATTGGGGGCCAAGTCTGTATCGCATCTTGAGTCCCTTTTTACC10460.162578627593914No Hit
TAATATACCCGCCTTCCATAGAGTGTGTAAATAATGTCTGGTTTGGAAGT10250.15931462073017388No Hit
CACCAAGACATTATCAAAAAATGTGAACAATTTGTAGGCCCACTCACAGT10140.15760490284916714No Hit
TCTGTGGAGTTACTCTCGTTTTTGCCTGCTGACTTCTTTCCTTCAGTACG10110.15713861615434713No Hit
GGATAATAAGGTTTAATACCCTTATCCAATGGCAAATATTTGGTAACATT9890.15371918039233365No Hit
TTCTTCTACTGTTCCTGTCTTTAACCCTCATTGGAAAACACCCTCTTTTC9530.14812374005449336No Hit
ATCCAAATGTTACCAAATATTTGCCATTGGATAAGGGTATTAAACCTTAT9480.14734659556312668No Hit
GCTTTAGGGCATGGACATTGATCCTTATAAAGAATTTGGAGCTTCTGTGG9390.1459477354786666No Hit
AGTCTGTATCGCATCTTGAGTCCCTTTTTACCGCTGTTACCAATTTTCTT9090.1412848685304664No Hit
CTTTTACACAATGTGGTTATCCTGCTTTAATGCCCTTGTATGCCTGTATT9000.13988600844600632No Hit
CTCAAGCTCAGGGCATACTACAAACCTTGCCAGCAAATCCGCCTCCTGTC8930.13879800615809293No Hit
GGGTAAAGGTTCAGGTATTGTTTACACAGAAAGGCCTTGTAAGTTGGCGA8860.13771000387017956No Hit
GCCTAGCAGCCATGGAAACGACGTATATTTGCGGGAGAGAACAACAGAGT8770.1363111437857195No Hit
CAATAGGCCTGTTAACAGGAAGTTTTCTAAAACATTCTTTGATTTTCTGT8660.13460142590471277No Hit
GTGTAAATGTATATTAGGAAAAGAGGGTGTTTTCCAATGAGGGTTAAAGA8500.13211456353233933No Hit
ATATTAGTGTTAACATAACTGACTACTAGGTCCCTGGATGTTGGATCTTC8430.13102656124442594No Hit
GTGTAGTTTCTCTCTTATATAATATACCCGCCTTCCATAGAGTGTGTAAA8150.1266745520927724No Hit
CTGCTAGGCTGTGCTGCCAACTGGATCCTGCGCGGGACGTCCTTTGTTTA8030.12480940531349233No Hit
TCCTTGGACTCATAAGGTGGGAAACTTTACGGGGCTTTATTCTTCTACTG7970.12387683192385227No Hit
TTATTATCCAGAACATCTAGTTAATCATTACTTCCAAACCAGACATTATT7950.1235659741273056No Hit
GTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATTACAGAGAGTCCAA7900.1227888296359389No Hit
GTTCTGGATAATAAGGTTTAATACCCTTATCCAATGGCAAATATTTGGTA7760.12061282506011213No Hit
GTCAATGAGAAACGAAGACTGCAATTGATTATGCCTGCTAGGTTTTATCC7730.12014653836529211No Hit
CTACAAACCTTGCCAGCAAATCCGCCTCCTGTCTCTACCAATCGCCAGTC7630.1185922493825587No Hit
GTTATGTTAACACTAATATGGGCCTAAAGTTCAGGCAACTATTGTGGTTT7580.117815104891192No Hit
TCTCCATCTCGGGGATCTCAATGTTAGTATTCCTTGGACTCATAAGGTGG7540.11719338929809865No Hit
GATTATGCCTGCTAGGTTTTATCCAAATGTTACCAAATATTTGCCATTGG7500.11657167370500528No Hit
GTATTCCCCTGCTGGTGGCTCCAGTTCAGGAACAGTAAACCCTGTTCCGA7490.11641624480673195No Hit
ATGTAAGACCTTGGGCAAGAATTGGTGGGCGTTCACGGTGGTCTCCATGC7330.11392938243435849No Hit
ATAATATACCCGCCTTCCATAGAGTGTGTAAATAATGTCTGGTTTGGAAG7210.11206423565507842No Hit
CTATAAGCTGGAGGAGTGCGAATCCACACTCCAAAAGACACCAAATACTC7200.11190880675680506No Hit
ATATAAGAGAGAAACTACACATAGCGCCTCATTTTGTGGATCACCATATT7140.11097623336716503No Hit
AGCTGGAGGAGTGCGAATCCACACTCCAAAAGACACCAAATACTCTATGA7120.11066537557061834No Hit
TAACCACATTGTGTAAAAGGGGCAGCAAATCCCAAAAGACCCACAATACG7100.11035451777407167No Hit
CTCACAGTCAATGAGAAACGAAGACTGCAATTGATTATGCCTGCTAGGTT7070.10988823107925164No Hit
GTGTGTAAATAATGTCTGGTTTGGAAGTAATGATTAACTAGATGTTCTGG7060.1097328021809783No Hit
CTTCCATAGAGTGTGTAAATAATGTCTGGTTTGGAAGTAATGATTAACTA7050.10957737328270496No Hit
GTATTAAACCTTATTATCCAGAACATCTAGTTAATCATTACTTCCAAACCAGACATTATTTACACACTCTATGGA7010.10895565768961159No Hit
GTCCCGTGCTGGTGGTTGAAGATCCTGGAATTAGAGGACAAACGGGCAAC7000.10880022879133826No Hit
GTCCAAGAGTCCTCTTATGTAAGACCTTGGGCAAGAATTGGTGGGCGTTC6960.10817851319824488No Hit
TCCTCAGGCCATGCAGTGGAACTCCACAACCTTCCACCAAACTCTGCAAG6930.10771222650342487No Hit
TCACCATACTGCACTCAGGCAAGCAATTCTTTGCTGGGGGGACTTAATAA6860.1066242242155115No Hit
GTATCTCCCTGCTGGTGGCTCCAGTTCAGGAACAGTAAACCCTGTTCCGA6800.10569165082587147No Hit
ATATAGAGCTGAGGCGGTATCTAGAAGATCTCGTACTGAAGGAAAGAAGT6740.1047590774362314No Hit
CTCTTTACATTTTATGGGCTATGTCATTGGTAGCTATGGGTCATTGCCAC6610.10273850175867799No Hit
ATGTGTAGTTTCTCTCTTATATAATATACCCGCCTTCCATAGAGTGTGTA6600.10258307286040463No Hit
GTCTTACATAAGAGGACTCTTGGACTCTCTGTAATGTCAACGACCGACCT6540.1016504994707646No Hit
GTGTAAACAATACCTGAACCTTTACCCCGTTGCCCGGCAACGGCCAGGTC6460.10040706828457788No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)