FastQCFastQC Report
Fri 2 Sep 2022
EGAF00002490316

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002490316
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences368087
Sequences flagged as poor quality0
Sequence length35-151
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCCATAGAGTGTGTAAATAATGTCTGGTTTGGAAGTAATGATTAACTAGA34930.9489604359838844No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN28120.7639498270789242No Hit
CATTTACACCAAGATATTATCAAAAAATGTGAACAATTTGTAGGCCCACT17210.46755250796686654No Hit
CTCTAAGACTGTTTCTCTTCCAAAAGTGAGACAAGAAATGTGAAACCACA13300.3613276209157074No Hit
GGATAAGGGTATTAAACCTTATTATCCAGAACATCTAGTTAATCATTACT11970.3251948588241367No Hit
TCCAGAGAGTGTGTAAATAATGTCTGGTTTGGAAGTAATGATTAACTAGA11250.3056342658121586No Hit
CACTTACAGTTAATGAGAAAAGAAGACTACGATTGATTATGCCTGCTAGG10090.27411997707063823No Hit
GTTTAATACCCTTATCCAATGGCAAATATTTGGTAACATTTGGATAAAAC10080.27384830216769407No Hit
AGTCTGTACAGCATCTTGAGTCCCTTTTTACCGCTGTTACCAATTTTCTT9500.25809115779693387No Hit
ATCCAAATGTTACCAAATATTTGCCATTGGATAAGGGTATTAAACCTTAT8820.23961726439673228No Hit
GGATAATAAGGTTTAATACCCTTATCCAATGGCAAATATTTGGTAACATT8300.22549016944363698No Hit
GTGTAAATAATGTCTGGTTTGGAAGTAATGATTAACTAGATGTTCTGGAT8270.22467514473480452No Hit
CTTACAGTTAATGAGAAAAGAAGACTACGATTGATTATGCCTGCTAGGTT8070.21924164667592172No Hit
CCCTTATCCAATGGCAAATATTTGGTAACATTTGGATAAAACCTAGCAGG8060.2189699717729776No Hit
GTATTAAACCTTATTATCCAGAACATCTAGTTAATCATTACTTCCAAACC7770.21109139958759748No Hit
CTCTAGGACTGTTTCTCTTCCAAAAGTGAGACAAGAAATGTGAAACCACA7440.20212612779044087No Hit
CACTAATATGGGCCTAAAGTTCAGACAATTATTGTGGTTTCACATTTCTT7410.20131110308160843No Hit
CTCCAAAAGACACCAAATACTCTAAGACTGTTTCTCTTCCAAAAGTGAGA7330.19913770385805532No Hit
GTCTTATATAAGAGGACTCTTGGACTCTCTGTAATGTCAACGACCGACCT7130.1937042057991725No Hit
CACCAAGATATTATCAAAAAATGTGAACAATTTGTAGGCCCACTTACAGT7010.19044410696384279No Hit
CTCTTATATAATATACCCGCCTTCCATAGAGTGTGTAAATAATGTCTGGT6550.17794706142841232No Hit
CTTATATAATATACCCGCCTTCCATAGAGTGTGTAAATAATGTCTGGTTT6480.17604533710780332No Hit
GATTATGCCTGCTAGGTTTTATCCAAATGTTACCAAATATTTGCCATTGG6440.17495863749602675No Hit
GTGTAAAAGGGGCAGCAAAACCCAAAAGACCCACAATACGTTGACAGACC6260.17006848924303222No Hit
AGTCTAGACTCTGCGGTATTGTGAGGATTCTTGTCAACAAGAAAAACCCC5930.16110321744587558No Hit
CCCTCACAAAACAAAAAGATGGGGATACTCTTTACATTTTATGGGCTATG5610.15240962055166307No Hit
CTCTATGGAAGGCGGGTATATTATATAAGAGAGAAACAACACATAGCGCC5560.15105124603694234No Hit
GTTCTGGATAATAAGGTTTAATACCCTTATCCAATGGCAAATATTTGGTA5510.14969287152222166No Hit
GTTAATGAGAAAAGAAGACTACGATTGATTATGCCTGCTAGGTTTTATCC5440.14779114720161265No Hit
GTGCTGGTGCGCAGACCAATTTATGCCTACAGCCTCCTAATACATAAATC5360.14561774797805954No Hit
TTGTTAGGGTTCAAATGTATACCCAAAGACAAAAGAAAATTGGTAACAGC5270.14317267385156227No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGGAACTACCGTG5250.142629324045674No Hit
CTCTTTACATTTTATGGGCTATGTCATTGGATGTTATGGGTCATTGCCAC5190.14099927462800915No Hit
GGTTTAATACCCTTATCCAATGGCAAATATTTGGTAACATTTGGATAAAA5090.13828252559856774No Hit
AATATACATTTACACCAAGATATTATCAAAAAATGTGAACAATTTGTAGG5050.13719582598679117No Hit
GTACAGCATCTTGAGTCCCTTTTTACCGCTGTTACCAATTTTCTTTTGTC5000.13583745147207046No Hit
ATATAATATACCCGCCTTCCATAGAGTGTGTAAATAATGTCTGGTTTGGA4960.1347507518602939No Hit
CTACTGTACCTGTCTTTAACCCTCATTGGAAAACACCCTCTTTTCCTAAT4930.1339357271514615No Hit
GTTTTATCCAAATGTTACCAAATATTTGCCATTGGATAAGGGTATTAAAC4850.13176232792790835No Hit
CCAAGATATTATCAAAAAATGTGAACAATTTGTAGGCCCACTTACAGTTA4790.1301322785102435No Hit
GATATTATCAAAAAATGTGAACAATTTGTAGGCCCACTTACAGTTAATGA4750.12904557889846693No Hit
GGGTAAAGGTTCAGGTATTGTTTACACAGAAAGGTCTTATAAGCTGGCGA4720.12823055418963453No Hit
GAAAAGAAGACTACGATTGATTATGCCTGCTAGGTTTTATCCAAATGTTA4650.12632882986902552No Hit
CAGTTAATGAGAAAAGAAGACTACGATTGATTATGCCTGCTAGGTTTTAT4610.12524213025724898No Hit
GTATTAAACCTTATTATCCAGAACATCTAGTTAATCATTACTTCCAAACCAGACATTATTTACACACTCTATGGA4590.12469878045136068No Hit
ATTTGTAGGCCCACTTACAGTTAATGAGAAAAGAAGACTACGATTGATTA4520.1227970561307517No Hit
ATATTTGCCATTGGATAAGGGTATTAAACCTTATTATCCAGAACATCTAG4500.1222537063248634No Hit
TCCATAGAGTGTGTAAATAATGTCTGGTTTGGAAGTAATGATTAACTAGATGTTCTGGATAATAAGGTTTAATAC4400.119536957295422No Hit
GGCATAATCAATCGTAGTCTTCTTTTCTCATTAACTGTAAGTGGGCCTAC4380.11899360748953372No Hit
GTGTAAATGTATATTAGGAAAAGAGGGTGTTTTCCAATGAGGGTTAAAGA4220.11464680904242747No Hit
TTATTATCCAGAACATCTAGTTAATCATTACTTCCAAACCAGACATTATT4190.11383178433359506No Hit
CTAATATACATTTACACCAAGATATTATCAAAAAATGTGAACAATTTGTA4030.1094849858864888No Hit
CTTTTACACAATGTGGTTATCCTGCTTTAATGCCCTTGTATGCATGTATT4000.10866996117765637No Hit
GGGTATTAAACCTTATTATCCAGAACATCTAGTTAATCATTACTTCCAAA3980.10812661137176809No Hit
TTAATACCCTTATCCAATGGCAAATATTTGGTAACATTTGGATAAAACCT3930.10676823685704738No Hit
CATTTGGATAAAACCTAGCAGGCATAATCAATCGTAGTCTTCTTTTCTCA3930.10676823685704738No Hit
GAATACATGCATACAAGGGCATTAAAGCAGGATAACCACATTGTGTAAAA3810.1035081380217177No Hit
ATATACATTTACACCAAGATATTATCAAAAAATGTGAACAATTTGTAGGC3760.10214976350699698No Hit
GTTATGTCAACACTAATATGGGCCTAAAGTTCAGACAATTATTGTGGTTT3760.10214976350699698No Hit
GTGTGTAAATAATGTCTGGTTTGGAAGTAATGATTAACTAGATGTTCTGG3740.1016064137011087No Hit
GGCAAATATTTGGTAACATTTGGATAAAACCTAGCAGGCATAATCAATCG3720.10106306389522043No Hit
TAATATACCCGCCTTCCATAGAGTGTGTAAATAATGTCTGGTTTGGAAGT3710.10079138899227628No Hit
ATTTATGCCTACAGCCTCCTAATACATAAATCATTAATCTAATCTCCTCC3700.10051971408933215No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)