FastQCFastQC Report
Sat 3 Sep 2022
EGAF00002490317

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002490317
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences368087
Sequences flagged as poor quality0
Sequence length35-151
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCCATAGAGTGTGTAAATAATGTCTGGTTTGGAAGTAATGATTAACTAGA34230.9299431927777944No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN28080.7628631274671477No Hit
CATTTACACCAAGATATTATCAAAAAATGTGAACAATTTGTAGGCCCACT15180.4124025026692059No Hit
CTCTAAGACTGTTTCTCTTCCAAAAGTGAGACAAGAAATGTGAAACCACA13350.3626859954304282No Hit
GTTTAATACCCTTATCCAATGGCAAATATTTGGTAACATTTGGATAAAAC11640.31622958702698006No Hit
TCCAGAGAGTGTGTAAATAATGTCTGGTTTGGAAGTAATGATTAACTAGA11080.30101579246210813No Hit
GGATAAGGGTATTAAACCTTATTATCCAGAACATCTAGTTAATCATTACT10650.2893337716355101No Hit
GGATAATAAGGTTTAATACCCTTATCCAATGGCAAATATTTGGTAACATT9820.2667847546911464No Hit
CCCTTATCCAATGGCAAATATTTGGTAACATTTGGATAAAACCTAGCAGG9610.26107958172931944No Hit
AGTCTGTACAGCATCTTGAGTCCCTTTTTACCGCTGTTACCAATTTTCTT9490.25781948289398976No Hit
CACTTACAGTTAATGAGAAAAGAAGACTACGATTGATTATGCCTGCTAGG8890.2415189887173413No Hit
ATCCAAATGTTACCAAATATTTGCCATTGGATAAGGGTATTAAACCTTAT8370.22739189376424596No Hit
GTGTAAATAATGTCTGGTTTGGAAGTAATGATTAACTAGATGTTCTGGAT8360.2271202188613018No Hit
CTCTAGGACTGTTTCTCTTCCAAAAGTGAGACAAGAAATGTGAAACCACA7410.20131110308160843No Hit
CTCCAAAAGACACCAAATACTCTAAGACTGTTTCTCTTCCAAAAGTGAGA7340.19940937876099943No Hit
CTTACAGTTAATGAGAAAAGAAGACTACGATTGATTATGCCTGCTAGGTT7240.19669262973155804No Hit
CACTAATATGGGCCTAAAGTTCAGACAATTATTGTGGTTTCACATTTCTT7000.19017243206089865No Hit
GTATTAAACCTTATTATCCAGAACATCTAGTTAATCATTACTTCCAAACC6860.18636898341968067No Hit
CTTATATAATATACCCGCCTTCCATAGAGTGTGTAAATAATGTCTGGTTT6750.18338055948729512No Hit
GGTTTAATACCCTTATCCAATGGCAAATATTTGGTAACATTTGGATAAAA6740.18310888458435098No Hit
GTCTTATATAAGAGGACTCTTGGACTCTCTGTAATGTCAACGACCGACCT6630.18012046065196544No Hit
CTCTTATATAATATACCCGCCTTCCATAGAGTGTGTAAATAATGTCTGGT6530.17740371162252402No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGGAACTACCGTG6360.17278523827247363No Hit
GTGTAAAAGGGGCAGCAAAACCCAAAAGACCCACAATACGTTGACAGACC6300.17115518885480877No Hit
GTGCTGGTGCGCAGACCAATTTATGCCTACAGCCTCCTAATACATAAATC6270.17034016414597636No Hit
GTTCTGGATAATAAGGTTTAATACCCTTATCCAATGGCAAATATTTGGTA6250.1697968143400881No Hit
GATTATGCCTGCTAGGTTTTATCCAAATGTTACCAAATATTTGCCATTGG6150.16708006531064667No Hit
CACCAAGATATTATCAAAAAATGTGAACAATTTGTAGGCCCACTTACAGT6060.1646349911841494No Hit
AGTCTAGACTCTGCGGTATTGTGAGGATTCTTGTCAACAAGAAAAACCCC6060.1646349911841494No Hit
CCCTCACAAAACAAAAAGATGGGGATACTCTTTACATTTTATGGGCTATG5570.15132292093988647No Hit
CTCTTTACATTTTATGGGCTATGTCATTGGATGTTATGGGTCATTGCCAC5560.15105124603694234No Hit
CTACTGTACCTGTCTTTAACCCTCATTGGAAAACACCCTCTTTTCCTAAT5430.14751947229866852No Hit
ATATAATATACCCGCCTTCCATAGAGTGTGTAAATAATGTCTGGTTTGGA5250.142629324045674No Hit
GTTAATGAGAAAAGAAGACTACGATTGATTATGCCTGCTAGGTTTTATCC5170.14045592482212085No Hit
TTGTTAGGGTTCAAATGTATACCCAAAGACAAAAGAAAATTGGTAACAGC5160.14018424991917672No Hit
CTCTATGGAAGGCGGGTATATTATATAAGAGAGAAACAACACATAGCGCC5150.13991257501623255No Hit
GTTTTATCCAAATGTTACCAAATATTTGCCATTGGATAAGGGTATTAAAC5040.13692415108384703No Hit
CTTTTACACAATGTGGTTATCCTGCTTTAATGCCCTTGTATGCATGTATT4470.121438681616031No Hit
GTATTAAACCTTATTATCCAGAACATCTAGTTAATCATTACTTCCAAACCAGACATTATTTACACACTCTATGGA4440.12062365690719858No Hit
GGCATAATCAATCGTAGTCTTCTTTTCTCATTAACTGTAAGTGGGCCTAC4430.12035198200425441No Hit
GGGTAAAGGTTCAGGTATTGTTTACACAGAAAGGTCTTATAAGCTGGCGA4400.119536957295422No Hit
TCCATAGAGTGTGTAAATAATGTCTGGTTTGGAAGTAATGATTAACTAGATGTTCTGGATAATAAGGTTTAATAC4380.11899360748953372No Hit
GGCAAATATTTGGTAACATTTGGATAAAACCTAGCAGGCATAATCAATCG4350.11817858278070129No Hit
GTACAGCATCTTGAGTCCCTTTTTACCGCTGTTACCAATTTTCTTTTGTC4350.11817858278070129No Hit
ATTTGTAGGCCCACTTACAGTTAATGAGAAAAGAAGACTACGATTGATTA4300.1168202082659806No Hit
GTGTGTAAATAATGTCTGGTTTGGAAGTAATGATTAACTAGATGTTCTGG4250.11546183375125989No Hit
GATATTATCAAAAAATGTGAACAATTTGTAGGCCCACTTACAGTTAATGA4240.11519015884831575No Hit
AATATACATTTACACCAAGATATTATCAAAAAATGTGAACAATTTGTAGG4200.1141034592365392No Hit
GGGTATTAAACCTTATTATCCAGAACATCTAGTTAATCATTACTTCCAAA4160.11301675962476262No Hit
CCAAGATATTATCAAAAAATGTGAACAATTTGTAGGCCCACTTACAGTTA4140.11247340981887434No Hit
ATTTATGCCTACAGCCTCCTAATACATAAATCATTAATCTAATCTCCTCC4130.1122017349159302No Hit
ATATTTGCCATTGGATAAGGGTATTAAACCTTATTATCCAGAACATCTAG4120.11193006001298607No Hit
GAAAAGAAGACTACGATTGATTATGCCTGCTAGGTTTTATCCAAATGTTA4080.1108433604012095No Hit
CATTTGGATAAAACCTAGCAGGCATAATCAATCGTAGTCTTCTTTTCTCA4070.11057168549826535No Hit
TTAATACCCTTATCCAATGGCAAATATTTGGTAACATTTGGATAAAACCT3990.10839828627471222No Hit
TTATTATCCAGAACATCTAGTTAATCATTACTTCCAAACCAGACATTATT3880.10540986234232669No Hit
CAGTTAATGAGAAAAGAAGACTACGATTGATTATGCCTGCTAGGTTTTAT3850.10459483763349425No Hit
GTGTAAATGTATATTAGGAAAAGAGGGTGTTTTCCAATGAGGGTTAAAGA3830.10405148782760597No Hit
GTTATGTCAACACTAATATGGGCCTAAAGTTCAGACAATTATTGTGGTTT3820.10377981292466183No Hit
CTGCTAGGCTGTGCTGCCAACTGGATCCTGCGCGGGACGTCCTTTGTTTA3780.10269311331288526No Hit
CCTTCCAATCAATAGGCCTGTTAACAGGAAGTTTTCTAAAACATTCTTTG3770.10242143840994113No Hit
TAATATACCCGCCTTCCATAGAGTGTGTAAATAATGTCTGGTTTGGAAGT3740.1016064137011087No Hit
ATATTAGTGTTGACATAACTGACTACTAGGTCTCTGGATGTTGGGTCTTC3710.10079138899227628No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)