FastQCFastQC Report
Sat 3 Sep 2022
EGAF00002490318

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002490318
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences396873
Sequences flagged as poor quality0
Sequence length35-151
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCCATAGAGTGTGTAAATAATGTCTGGTTTGGAAGTAATGATTAACTAGA33780.8511538955786864No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN16400.4132304288777518No Hit
CTCTATGGAAGGCGGGTATATTATATAAGAGAGAAACTACACATAGCGCC11560.29127705840407386No Hit
AGTCTAGACTCTGCGGTATTGTGAGGATTCTTGTCAACAAGAAAAACCCC11460.2887573606670144No Hit
CTTATATAATATACCCGCCTTCCATAGAGTGTGTAAATAATGTCTGGTTT11340.285733723382543No Hit
AGATAAGGGTATTAAACCTTATTATCCAGAACATCTAGTTAATCATTACT11290.2844738745140133No Hit
CTCTTATATAATATACCCGCCTTCCATAGAGTGTGTAAATAATGTCTGGT11110.2799384185873063No Hit
CATTTACACCAAGACATTATCAAAAAATGTGAACAATTTGTAGGCCCACT10970.276410841755423No Hit
AGTCTGTACAGCATCTTGAGTCCCTTTTTACCGCTGTTACCAATTTTCTT9110.229544463846117No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGGAACTACCGTG9000.2267727963353516No Hit
GTGTAAAAGGGGCAGCAAAACCCAAAAGACCCACAATACGTTGACAGACT9000.2267727963353516No Hit
GTATTAAACCTTATTATCCAGAACATCTAGTTAATCATTACTTCCAAACC8980.22626885678793973No Hit
GTGCTGGTGCGCAGACCAATTTATGCCTACAGCCTCCTAGTACAAAGATC8890.22400112882458623No Hit
ATATAATATACCCGCCTTCCATAGAGTGTGTAAATAATGTCTGGTTTGGA8520.2146782471974662No Hit
GTGTAAATAATGTCTGGTTTGGAAGTAATGATTAACTAGATGTTCTGGAT8470.21341839832893644No Hit
CATTTAAACCCTAACAAAACAAAAAGATGGGGTTACTCTTTACATTTCAT8200.2066152144388759No Hit
GTTTAATACCCTTATCTAATGGCAAATATTTGGTAACCTTTGGATAAAAC8110.2043474864755224No Hit
ATCCAAAGGTTACCAAATATTTGCCATTAGATAAGGGTATTAAACCTTAT7750.19527657462210835No Hit
GGATAATAAGGTTTAATACCCTTATCTAATGGCAAATATTTGGTAACCTT6740.16982762747780775No Hit
GTGTAGTTTCTCTCTTATATAATATACCCGCCTTCCATAGAGTGTGTAAA6550.16504020177739478No Hit
GGGTATACATTTAAACCCTAACAAAACAAAAAGATGGGGTTACTCTTTAC6500.16378035290886506No Hit
CCCTTATCTAATGGCAAATATTTGGTAACCTTTGGATAAAACCTAGCAGG6400.1612606551718056No Hit
TAATATACCCGCCTTCCATAGAGTGTGTAAATAATGTCTGGTTTGGAAGT6400.1612606551718056No Hit
CTATGTATCCCTCCTGTTGCTGTACAAAACCTTCGGACGGAAATTGCACC5930.14941807580762612No Hit
CCTCCAGCTTATAGACCACCAAATGCCCCTATCTTATCAACACTTCCGGA5570.14034716395421204No Hit
TTATTATCCAGAACATCTAGTTAATCATTACTTCCAAACCAGACATTATT5560.14009519418050612No Hit
CTACAAACCTTGCCAGCAAATCCGCCTCCTGCCTCTACCAATCGCCAGTC5360.13505579870638718No Hit
ATGTGTAGTTTCTCTCTTATATAATATACCCGCCTTCCATAGAGTGTGTA5330.13429988938526935No Hit
CAATAGGCCTGTTAACAGGAAGTTTTCTAAAACATTCTTTGATTTTCTGT5280.13304004051673962No Hit
GTGCTGGTGGGAGTGTAGCAGTGAGGACGACCAGAAGTCACTCTCGTGGC5270.13278807074303367No Hit
GGGTAAAGGTTCATGTATTGTTTACACAGAAAGGCCTTGTAAGTTGGCGA5240.13203216142191584No Hit
GTACAGACTTGGCCCCCAATACCACATCATCCATATAACTGAAAGCCAAA5240.13203216142191584No Hit
CACCAAGACATTATCAAAAAATGTGAACAATTTGTAGGCCCACTCACAGT5160.13001640323226826No Hit
GAAAAGAAGACTGCAATTGATTATGCCTGCTAGGTTTTATCCAAAGGTTA5100.12850458459003258No Hit
GTTTTATCCAAAGGTTACCAAATATTTGCCATTAGATAAGGGTATTAAAC4860.12245731002108987No Hit
GTACAGCATCTTGAGTCCCTTTTTACCGCTGTTACCAATTTTCTTTTGTC4840.12195337047367799No Hit
CTCACAGTCAATGAGAAAAGAAGACTGCAATTGATTATGCCTGCTAGGTT4790.12069352160514824No Hit
GAGATACAGTAGTCGGAACAGGGTTTACTGTTCCTGAACTGGAGCCACCA4760.11993761228403041No Hit
ATATAGAGCTGAGGCGGTATCTAGAAGATCTCGTACTGAAGGAAAGAAGT4740.1194336727366185No Hit
GTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATTACAGCGAGTCCAA4710.11867776341550067No Hit
GATTATGCCTGCTAGGTTTTATCCAAAGGTTACCAAATATTTGCCATTAG4690.11817382386808879No Hit
AATATACATTTACACCAAGACATTATCAAAAAATGTGAACAATTTGTAGG4610.11615806567844122No Hit
GGATAACCACATTGTGTAAAAGGGGCAGCAAAACCCAAAAGACCCACAAT4580.11540215635732337No Hit
GTCAATGAGAAAAGAAGACTGCAATTGATTATGCCTGCTAGGTTTTATCC4530.11414230748879364No Hit
ATAATATACCCGCCTTCCATAGAGTGTGTAAATAATGTCTGGTTTGGAAG4520.1138903377150877No Hit
CTTTAGGACATGGACATTGATCCCTATAAAGAATTTGGAGCTTCTGTGGA4500.1133863981676758No Hit
TCCTTGGACTCATAAGGTGGGAAACTTTACGGGGCTTTATTCTTCTTCTG4470.11263048884655796No Hit
GGGAAATACAGGCCTTTCACTCTGGGATCTTGCAGAGTTTGATGGAAGGT4440.11187457952544014No Hit
CACTAATATGGGCCTAAAGTTCAGGCAACTATTGTGGTTTCACATTTCTT4430.11162260975173417No Hit
ATATAAGAGAGAAACTACACATAGCGCCTCATTTTGTGGATCACCATATT4430.11162260975173417No Hit
ATATTAGTGTTAACATAACTGACTACTAGGTCCCTGGAGACTGGATCTTG4320.10885094224096878No Hit
TAACCACATTGTGTAAAAGGGGCAGCAAAACCCAAAAGACCCACAATACG4200.10582730495649743No Hit
GTGTGTAAATAATGTCTGGTTTGGAAGTAATGATTAACTAGATGTTCTGG4150.10456745608796768No Hit
CTCATAAGGTGGGAAACTTTACGGGGCTTTATTCTTCTTCTGTTCCTGTC4150.10456745608796768No Hit
GTTTCCTAAAACATTCTTTGATTTTCTGTATGATGTGATCTTGTGGCAAT4120.10381154676684987No Hit
ATGTAAGACCTTGGGCAAGAATTGGTGGGCGTTCACGGTGGTCTCCATGC4110.1035595769931439No Hit
ATGTGGTATTGGGGGCCAAGTCTGTACAGCATCTTGAGTCCCTTTTTACC4060.10229972812461417No Hit
CAGTTATATGGATGATGTGGTATTGGGGGCCAAGTCTGTACAGCATCTTG4060.10229972812461417No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)